UniProtKB - P83917 (CBX1_MOUSE)
Chromobox protein homolog 1
Cbx1
Functioni
Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane.
2 PublicationsSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 21 | Histone H3K9me2 binding | 1 | |
Sitei | 23 | Histone H3A7 binding | 1 | |
Sitei | 40 | Histone H3A7 binding | 1 | |
Sitei | 42 | Histone H3A7 binding | 1 | |
Sitei | 42 | Histone H3K9me2 binding | 1 | |
Sitei | 45 | Histone H3K9me2 binding | 1 | |
Sitei | 58 | Histone H3A7 binding | 1 | |
Sitei | 60 | Histone H3A7 binding | 1 | |
Sitei | 125 | Interacts with the PxVxL motif of TRIM28/TIF1B | 1 | |
Sitei | 126 | Interacts with the PxVxL motif of TRIM28/TIF1B | 1 | |
Sitei | 135 | Interacts with the PxVxL motif of TRIM28/TIF1B | 1 | |
Sitei | 146 | Interacts with the PxVxL motif of TRIM28/TIF1B | 1 | |
Sitei | 163 | Interacts with the PxVxL motif of TRIM28/TIF1B | 1 | |
Sitei | 167 | Interacts with the PxVxL motif of TRIM28/TIF1B | 1 | |
Sitei | 168 | Interacts with the PxVxL motif of TRIM28/TIF1B | 1 | |
Sitei | 170 | Interacts with the PxVxL motif of TRIM28/TIF1B | 1 |
GO - Molecular functioni
- chromatin binding Source: GO_Central
- enzyme binding Source: MGI
- histone methyltransferase binding Source: MGI
- identical protein binding Source: IntAct
- methylated histone binding Source: GO_Central
GO - Biological processi
- cellular response to DNA damage stimulus Source: UniProtKB
- chromatin organization Source: GO_Central
- negative regulation of transcription, DNA-templated Source: MGI
- negative regulation of transcription by RNA polymerase II Source: GO_Central
Names & Taxonomyi
Protein namesi | Recommended name: Chromobox protein homolog 1Alternative name(s): Heterochromatin protein 1 homolog beta Short name: HP1 beta Heterochromatin protein p25 M31 Modifier 1 protein |
Gene namesi | Name:Cbx1 Synonyms:Cbx |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:105369, Cbx1 |
VEuPathDBi | HostDB:ENSMUSG00000018666 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Note: Unassociated with chromosomes during mitosis.By similarity
Cytoskeleton
- spindle Source: MGI
Nucleus
- female pronucleus Source: MGI
- male pronucleus Source: MGI
- nucleus Source: MGI
Other locations
- chromatin Source: MGI
- chromocenter Source: MGI
- chromosome, centromeric region Source: MGI
- chromosome, telomeric region Source: Ensembl
- pericentric heterochromatin Source: MGI
- site of DNA damage Source: UniProtKB
Keywords - Cellular componenti
NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 23 | V → M: Abolishes interaction with SUV39H1. 1 Publication | 1 | |
Mutagenesisi | 26 | V → R: Abolishes interaction with SUV39H1. 1 Publication | 1 | |
Mutagenesisi | 161 | I → E: Abolishes homodimer formation. 1 Publication | 1 | |
Mutagenesisi | 164 | Y → E: Abolishes homodimer formation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080200 | 1 – 185 | Chromobox protein homolog 1Add BLAST | 185 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 9 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 33 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 89 | PhosphoserineBy similarity | 1 | |
Modified residuei | 91 | PhosphoserineBy similarity | 1 | |
Cross-linki | 99 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 150 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 175 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P83917 |
jPOSTi | P83917 |
MaxQBi | P83917 |
PaxDbi | P83917 |
PeptideAtlasi | P83917 |
PRIDEi | P83917 |
ProteomicsDBi | 281232 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00129466 |
PTM databases
iPTMneti | P83917 |
PhosphoSitePlusi | P83917 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000018666, Expressed in vestibular organ and 345 other tissues |
ExpressionAtlasi | P83917, baseline and differential |
Genevisiblei | P83917, MM |
Interactioni
Subunit structurei
Homodimer (PubMed:10747027).
Interacts directly with CHAF1A, EMSY, LBR, TIF1/TIF1A and TRIM28/TIF1B PXVXL motif via the chromoshadow domain (PubMed:8978696, PubMed:10562550, PubMed:10747027).
Interacts directly with histone H3 methylated at 'Lys-9' via the chromo domain (PubMed:11571267).
Interacts with SUV39H1, SETDB1, KMT5B and KMT5C (PubMed:10202156, PubMed:15145825).
Interacts with PRDM6 (PubMed:16537907).
Interacts with POGZ (By similarity).
Interacts with CHAMP1 (By similarity).
Interacts with INCENP (By similarity).
Interacts with SGO1; the CBX1 homodimer binds to one molecule of SGO1 (By similarity).
Interacts with LRIF1 (via PxVxL motif) (By similarity).
Interacts with HDGFL2 (By similarity).
By similarity7 PublicationsBinary interactionsi
P83917
GO - Molecular functioni
- enzyme binding Source: MGI
- histone methyltransferase binding Source: MGI
- identical protein binding Source: IntAct
- methylated histone binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 198534, 211 interactors |
DIPi | DIP-30892N |
IntActi | P83917, 156 interactors |
MINTi | P83917 |
STRINGi | 10090.ENSMUSP00000091475 |
Miscellaneous databases
RNActi | P83917, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P83917 |
SMRi | P83917 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P83917 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 21 – 79 | Chromo 1PROSITE-ProRule annotationAdd BLAST | 59 | |
Domaini | 117 – 175 | Chromo 2; shadow subtypePROSITE-ProRule annotationAdd BLAST | 59 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 63 – 124 | DisorderedSequence analysisAdd BLAST | 62 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 72 – 116 | Basic and acidic residuesSequence analysisAdd BLAST | 45 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG1911, Eukaryota |
GeneTreei | ENSGT00940000154152 |
HOGENOMi | CLU_045874_1_0_1 |
InParanoidi | P83917 |
OMAi | SNEENTW |
OrthoDBi | 1628171at2759 |
PhylomeDBi | P83917 |
TreeFami | TF350503 |
Family and domain databases
DisProti | DP02864 |
InterProi | View protein in InterPro IPR016197, Chromo-like_dom_sf IPR000953, Chromo/chromo_shadow_dom IPR017984, Chromo_dom_subgr IPR023780, Chromo_domain IPR008251, Chromo_shadow_dom IPR023779, Chromodomain_CS |
Pfami | View protein in Pfam PF00385, Chromo, 1 hit PF01393, Chromo_shadow, 1 hit |
PRINTSi | PR00504, CHROMODOMAIN |
SMARTi | View protein in SMART SM00298, CHROMO, 2 hits SM00300, ChSh, 1 hit |
SUPFAMi | SSF54160, SSF54160, 2 hits |
PROSITEi | View protein in PROSITE PS00598, CHROMO_1, 1 hit PS50013, CHROMO_2, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MGKKQNKKKV EEVLEEEEEE YVVEKVLDRR VVKGKVEYLL KWKGFSDEDN
60 70 80 90 100
TWEPEENLDC PDLIAEFLQS QKTAHETDKS EGGKRKADSD SEDKGEESKP
110 120 130 140 150
KKKKEESEKP RGFARGLEPE RIIGATDSSG ELMFLMKWKN SDEADLVPAK
160 170 180
EANVKCPQVV ISFYEERLTW HSYPSEDDDK KDDKN
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ7TPM0 | Q7TPM0_MOUSE | Cbx1 protein | Cbx1 | 138 | Annotation score: | ||
Q9CYJ8 | Q9CYJ8_MOUSE | Chromobox protein homolog 1 | Cbx1 | 150 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X56690 mRNA Translation: CAA40018.1 AK014215 mRNA Translation: BAB29211.1 |
CCDSi | CCDS25303.1 |
PIRi | S26847 |
RefSeqi | NP_031648.1, NM_007622.3 XP_006532162.1, XM_006532099.2 XP_006532163.1, XM_006532100.2 XP_011247006.1, XM_011248704.2 XP_017169731.1, XM_017314242.1 |
Genome annotation databases
Ensembli | ENSMUST00000093943; ENSMUSP00000091475; ENSMUSG00000018666 |
GeneIDi | 12412 |
KEGGi | mmu:12412 |
UCSCi | uc007lck.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X56690 mRNA Translation: CAA40018.1 AK014215 mRNA Translation: BAB29211.1 |
CCDSi | CCDS25303.1 |
PIRi | S26847 |
RefSeqi | NP_031648.1, NM_007622.3 XP_006532162.1, XM_006532099.2 XP_006532163.1, XM_006532100.2 XP_011247006.1, XM_011248704.2 XP_017169731.1, XM_017314242.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1AP0 | NMR | - | A | 10-80 | [»] | |
1DZ1 | NMR | - | A/B | 104-171 | [»] | |
1GUW | NMR | - | A | 10-80 | [»] | |
1S4Z | NMR | - | A/B | 104-176 | [»] | |
AlphaFoldDBi | P83917 | |||||
SMRi | P83917 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 198534, 211 interactors |
DIPi | DIP-30892N |
IntActi | P83917, 156 interactors |
MINTi | P83917 |
STRINGi | 10090.ENSMUSP00000091475 |
PTM databases
iPTMneti | P83917 |
PhosphoSitePlusi | P83917 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00129466 |
Proteomic databases
EPDi | P83917 |
jPOSTi | P83917 |
MaxQBi | P83917 |
PaxDbi | P83917 |
PeptideAtlasi | P83917 |
PRIDEi | P83917 |
ProteomicsDBi | 281232 |
Protocols and materials databases
Antibodypediai | 3221, 350 antibodies from 39 providers |
DNASUi | 12412 |
Genome annotation databases
Ensembli | ENSMUST00000093943; ENSMUSP00000091475; ENSMUSG00000018666 |
GeneIDi | 12412 |
KEGGi | mmu:12412 |
UCSCi | uc007lck.1, mouse |
Organism-specific databases
CTDi | 10951 |
MGIi | MGI:105369, Cbx1 |
VEuPathDBi | HostDB:ENSMUSG00000018666 |
Phylogenomic databases
eggNOGi | KOG1911, Eukaryota |
GeneTreei | ENSGT00940000154152 |
HOGENOMi | CLU_045874_1_0_1 |
InParanoidi | P83917 |
OMAi | SNEENTW |
OrthoDBi | 1628171at2759 |
PhylomeDBi | P83917 |
TreeFami | TF350503 |
Miscellaneous databases
BioGRID-ORCSi | 12412, 5 hits in 75 CRISPR screens |
ChiTaRSi | Cbx1, mouse |
EvolutionaryTracei | P83917 |
PROi | PR:P83917 |
RNActi | P83917, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000018666, Expressed in vestibular organ and 345 other tissues |
ExpressionAtlasi | P83917, baseline and differential |
Genevisiblei | P83917, MM |
Family and domain databases
DisProti | DP02864 |
InterProi | View protein in InterPro IPR016197, Chromo-like_dom_sf IPR000953, Chromo/chromo_shadow_dom IPR017984, Chromo_dom_subgr IPR023780, Chromo_domain IPR008251, Chromo_shadow_dom IPR023779, Chromodomain_CS |
Pfami | View protein in Pfam PF00385, Chromo, 1 hit PF01393, Chromo_shadow, 1 hit |
PRINTSi | PR00504, CHROMODOMAIN |
SMARTi | View protein in SMART SM00298, CHROMO, 2 hits SM00300, ChSh, 1 hit |
SUPFAMi | SSF54160, SSF54160, 2 hits |
PROSITEi | View protein in PROSITE PS00598, CHROMO_1, 1 hit PS50013, CHROMO_2, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | CBX1_MOUSE | |
Accessioni | P83917Primary (citable) accession number: P83917 Secondary accession number(s): P23197 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2004 |
Last sequence update: | June 7, 2004 | |
Last modified: | May 25, 2022 | |
This is version 170 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references