UniProtKB - P83571 (RUVB2_DANRE)
RuvB-like 2
ruvbl2
Functioni
Has double-stranded DNA-stimulated ATPase activity (PubMed:12464178).
Has ATP-dependent DNA helicase (5' to 3') activity suggesting a role in nuclear processes such as recombination and transcription (By similarity).
Represses gene activation mediated by beta-catenin (PubMed:12464178).
Proposed core component of the chromatin remodeling Ino80 complex which exhibits DNA- and nucleosome-activated ATPase activity and catalyzes ATP-dependent nucleosome sliding (By similarity).
Involved in the endoplasmic reticulum (ER)-associated degradation (ERAD) pathway where it negatively regulates expression of ER stress response genes (By similarity).
May act as a regulator of embryonic heart growth (PubMed:12464178).
By similarity1 PublicationCatalytic activityi
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 77 – 84 | ATPBy similarity | 8 |
GO - Molecular functioni
- 5'-3' DNA helicase activity Source: InterPro
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: UniProtKB
- DNA helicase activity Source: UniProtKB
GO - Biological processi
- axonemal dynein complex assembly Source: ZFIN
- box C/D snoRNP assembly Source: GO_Central
- chromatin remodeling Source: GO_Central
- digestive tract development Source: ZFIN
- DNA recombination Source: UniProtKB-KW
- DNA repair Source: UniProtKB-KW
- epithelial cilium movement involved in extracellular fluid movement Source: ZFIN
- heart development Source: ZFIN
- histone acetylation Source: GO_Central
- motile cilium assembly Source: ZFIN
- negative regulation of transcription, DNA-templated Source: UniProtKB
- regulation of heart growth Source: ZFIN
- regulation of transcription by RNA polymerase II Source: GO_Central
Keywordsi
Molecular function | Helicase, Hydrolase |
Biological process | DNA damage, DNA recombination, DNA repair, Transcription, Transcription regulation |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ruvbl2 |
Organismi | Danio rerio (Zebrafish) (Brachydanio rerio)Imported |
Taxonomic identifieri | 7955 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Danionidae › Danioninae › Danio |
Proteomesi |
|
Organism-specific databases
ZFINi | ZDB-GENE-030109-1, ruvbl2 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Other locations
- Dynein axonemal particle By similarity
Extracellular region or secreted
- extracellular region Source: GOC
Nucleus
- Ino80 complex Source: GO_Central
- MLL1 complex Source: UniProtKB
- NuA4 histone acetyltransferase complex Source: GO_Central
- nucleus Source: UniProtKB
- Swr1 complex Source: UniProtKB
Other locations
- dynein axonemal particle Source: UniProtKB
- R2TP complex Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 190 | G → GFCR in LIK mutant; causes embryonic cardiac hyperplasia. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000165646 | 1 – 463 | RuvB-like 2Add BLAST | 463 |
Proteomic databases
PaxDbi | P83571 |
PRIDEi | P83571 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | ENSDARG00000055639, Expressed in testis and 39 other tissues |
Interactioni
Subunit structurei
Forms homohexameric rings (Probable). Can form a dodecamer with ruvbl1 made of two stacked hexameric rings (By similarity).
Component of the chromatin-remodeling Ino80 complex (By similarity).
Component of some MLL1/MLL complex (By similarity).
By similarityCuratedProtein-protein interaction databases
STRINGi | 7955.ENSDARP00000072482 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2680, Eukaryota |
GeneTreei | ENSGT00940000153556 |
HOGENOMi | CLU_028311_4_0_1 |
InParanoidi | P83571 |
OMAi | YDMGAKM |
OrthoDBi | 752343at2759 |
PhylomeDBi | P83571 |
TreeFami | TF300469 |
Family and domain databases
Gene3Di | 2.40.50.360, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR027417, P-loop_NTPase IPR027238, RuvB-like IPR041048, RuvB-like_C IPR042487, RuvBL1/2_DNA/RNA_bd_dom IPR037942, RUVBL2 IPR010339, TIP49_P-loop |
PANTHERi | PTHR11093, PTHR11093, 1 hit PTHR11093:SF2, PTHR11093:SF2, 1 hit |
Pfami | View protein in Pfam PF06068, TIP49, 1 hit PF17856, TIP49_C, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MAAQVATTKV PEVRDITRIE RIGAHSHIRG LGLDDALEPR QVSQGMVGQL
60 70 80 90 100
ASRRAAGLIL EMIKDGQIAG RAVLIAGQPG TGKTAIAMGI AQSLGPDTPF
110 120 130 140 150
TALAGSEIFS LEMSKTEALS QAFRKAIGVR IKEETEIIEG EVVEIQIDRP
160 170 180 190 200
ATGTGAKVGK LTLKTTEMET IYDLGTKMIE SLSKERVQAG DVITIDKATG
210 220 230 240 250
KISKLGRSFT RARDYDAMGA QTQFVQCPEG ELQKRKEVVH TVSLHEIDVI
260 270 280 290 300
NSRTQGFLAL FSGDTGEIKS EVREQINAKV SEWREEGKAE IIPGVLFIDE
310 320 330 340 350
VHMLDIECFS FLNRALESDL SPVLIMATNR GITRIRGTNY QSPHGIPIDM
360 370 380 390 400
LDRLLIIATT PYTEKETRQI LKIRCEEEDV ELSEEAHTVL TRIGQETSLR
410 420 430 440 450
YAIQLISTAG LVCRKRRGTE VQVEDIKRVY SLFLDEARSS QYMKEYQDSF
460
LFNETQTSQM DTS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A2R8QIK4 | A0A2R8QIK4_DANRE | RuvB-like helicase | ruvbl2 | 454 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY057075 mRNA Translation: AAL18005.1 BC058871 mRNA Translation: AAH58871.1 |
RefSeqi | NP_777285.1, NM_174860.2 |
Genome annotation databases
Ensembli | ENSDART00000078018; ENSDARP00000072482; ENSDARG00000055639 |
GeneIDi | 317678 |
KEGGi | dre:317678 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY057075 mRNA Translation: AAL18005.1 BC058871 mRNA Translation: AAH58871.1 |
RefSeqi | NP_777285.1, NM_174860.2 |
3D structure databases
SMRi | P83571 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 7955.ENSDARP00000072482 |
Proteomic databases
PaxDbi | P83571 |
PRIDEi | P83571 |
Genome annotation databases
Ensembli | ENSDART00000078018; ENSDARP00000072482; ENSDARG00000055639 |
GeneIDi | 317678 |
KEGGi | dre:317678 |
Organism-specific databases
CTDi | 10856 |
ZFINi | ZDB-GENE-030109-1, ruvbl2 |
Phylogenomic databases
eggNOGi | KOG2680, Eukaryota |
GeneTreei | ENSGT00940000153556 |
HOGENOMi | CLU_028311_4_0_1 |
InParanoidi | P83571 |
OMAi | YDMGAKM |
OrthoDBi | 752343at2759 |
PhylomeDBi | P83571 |
TreeFami | TF300469 |
Miscellaneous databases
PROi | PR:P83571 |
Gene expression databases
Bgeei | ENSDARG00000055639, Expressed in testis and 39 other tissues |
Family and domain databases
Gene3Di | 2.40.50.360, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR027417, P-loop_NTPase IPR027238, RuvB-like IPR041048, RuvB-like_C IPR042487, RuvBL1/2_DNA/RNA_bd_dom IPR037942, RUVBL2 IPR010339, TIP49_P-loop |
PANTHERi | PTHR11093, PTHR11093, 1 hit PTHR11093:SF2, PTHR11093:SF2, 1 hit |
Pfami | View protein in Pfam PF06068, TIP49, 1 hit PF17856, TIP49_C, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RUVB2_DANRE | |
Accessioni | P83571Primary (citable) accession number: P83571 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 19, 2003 |
Last sequence update: | June 1, 2003 | |
Last modified: | February 23, 2022 | |
This is version 123 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families