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Protein

Glucose-6-phosphate isomerase

Gene

pgiA

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glucose 6-phosphate = D-fructose 6-phosphate.

Cofactori

Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity

Activity regulationi

Inhibited by mannose 6-phosphate, fructose 1-phosphate and fructose 1,6-bisphosphate.

pH dependencei

Optimum pH is 7.0.

Temperature dependencei

Optimum temperature is 90-96 degrees Celsius. Highly thermostable.

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgiA)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi88IronBy similarity1
Metal bindingi90IronBy similarity1
Metal bindingi97IronBy similarity1
Metal bindingi136IronBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase
Biological processGluconeogenesis, Glycolysis
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-11807
BRENDAi5.3.1.9 5243
SABIO-RKiP83194
UniPathwayi
UPA00109;UER00181

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate isomerase (EC:5.3.1.9)
Short name:
GPI
Alternative name(s):
Phosphoglucose isomerase
Short name:
PGI
Phosphohexose isomerase
Short name:
PHI
Gene namesi
Name:pgiA
Ordered Locus Names:PF0196
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001853571 – 189Glucose-6-phosphate isomeraseAdd BLAST189

Proteomic databases

PRIDEiP83194

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi186497.PF0196

Structurei

Secondary structure

1189
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP83194
SMRiP83194
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP83194

Family & Domainsi

Sequence similaritiesi

Belongs to the archaeal-type GPI family.Curated

Phylogenomic databases

eggNOGiarCOG02602 Archaea
COG2140 LUCA
HOGENOMiHOG000243903
KOiK06859
OMAiYPADAGH
OrthoDBiPOG093Z0BUP

Family and domain databases

Gene3Di2.60.120.10, 1 hit
HAMAPiMF_01410 G6P_isomerase_arch, 1 hit
InterProiView protein in InterPro
IPR016758 G6P_isomerase_archaea/bacteria
IPR010551 G6P_isomerase_prok
IPR014710 RmlC-like_jellyroll
IPR011051 RmlC_Cupin_sf
PfamiView protein in Pfam
PF06560 GPI, 1 hit
PIRSFiPIRSF019325 Glucose-6-phosphate_isomerase, 1 hit
SUPFAMiSSF51182 SSF51182, 1 hit

Sequencei

Sequence statusi: Complete.

P83194-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MYKEPFGVKV DFETGIIEGA KKSVRRLSDM EGYFVDERAW KELVEKEDPV
60 70 80 90 100
VYEVYAVEQE EKEGDLNFAT TVLYPGKVGK EFFFTKGHFH AKLDRAEVYV
110 120 130 140 150
ALKGKGGMLL QTPEGDAKWI SMEPGTVVYV PPYWAHRTVN IGDEPFIFLA
160 170 180
IYPADAGHDY GTIAEKGFSK IVIEENGEVK VVDNPRWKK
Length:189
Mass (Da):21,476
Last modified:March 1, 2002 - v1
Checksum:iC5D8380C59F2A785
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11D → N AA sequence (PubMed:11533028).Curated1
Sequence conflicti28S → P AA sequence (PubMed:11344151).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF381250 Genomic DNA Translation: AAL27992.1
AE009950 Genomic DNA Translation: AAL80320.1
RefSeqiWP_011011309.1, NC_003413.1

Genome annotation databases

EnsemblBacteriaiAAL80320; AAL80320; PF0196
GeneIDi1468028
KEGGipfu:PF0196
PATRICifig|186497.12.peg.203

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF381250 Genomic DNA Translation: AAL27992.1
AE009950 Genomic DNA Translation: AAL80320.1
RefSeqiWP_011011309.1, NC_003413.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QXJX-ray1.80A/B1-189[»]
1QXRX-ray1.70A/B1-189[»]
1QY4X-ray1.80A/B1-189[»]
1X7NX-ray1.89A1-189[»]
1X82X-ray1.50A1-189[»]
1X8EX-ray2.80A/B1-189[»]
2GC0X-ray2.00A/B1-187[»]
2GC1X-ray1.95A/B1-187[»]
2GC2X-ray2.10A/B1-187[»]
2GC3X-ray2.10A/B1-187[»]
3SXWX-ray1.80A1-189[»]
4LTAX-ray2.04A/B1-189[»]
4LUKX-ray1.41A1-189[»]
4LULX-ray1.89A/B1-189[»]
4LUMX-ray1.79A/B1-189[»]
ProteinModelPortaliP83194
SMRiP83194
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF0196

Proteomic databases

PRIDEiP83194

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL80320; AAL80320; PF0196
GeneIDi1468028
KEGGipfu:PF0196
PATRICifig|186497.12.peg.203

Phylogenomic databases

eggNOGiarCOG02602 Archaea
COG2140 LUCA
HOGENOMiHOG000243903
KOiK06859
OMAiYPADAGH
OrthoDBiPOG093Z0BUP

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00181

BioCyciMetaCyc:MONOMER-11807
BRENDAi5.3.1.9 5243
SABIO-RKiP83194

Miscellaneous databases

EvolutionaryTraceiP83194

Family and domain databases

Gene3Di2.60.120.10, 1 hit
HAMAPiMF_01410 G6P_isomerase_arch, 1 hit
InterProiView protein in InterPro
IPR016758 G6P_isomerase_archaea/bacteria
IPR010551 G6P_isomerase_prok
IPR014710 RmlC-like_jellyroll
IPR011051 RmlC_Cupin_sf
PfamiView protein in Pfam
PF06560 GPI, 1 hit
PIRSFiPIRSF019325 Glucose-6-phosphate_isomerase, 1 hit
SUPFAMiSSF51182 SSF51182, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiG6PI_PYRFU
AccessioniPrimary (citable) accession number: P83194
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: March 1, 2002
Last modified: October 10, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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