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Protein

Pancreatic alpha-amylase

Gene
N/A
Organism
Struthio camelus (Common ostrich)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.1 Publication

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi100CalciumBy similarity1
Metal bindingi158Calcium; via carbonyl oxygenBy similarity1
Metal bindingi167CalciumBy similarity1
Binding sitei195ChlorideBy similarity1
Active sitei197NucleophileBy similarity1
Metal bindingi201Calcium; via carbonyl oxygenBy similarity1
Active sitei233Proton donorBy similarity1
Binding sitei298ChlorideBy similarity1
Sitei300Transition state stabilizerBy similarity1
Binding sitei337ChlorideBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCarbohydrate metabolism
LigandCalcium, Chloride, Metal-binding

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Names & Taxonomyi

Protein namesi
Recommended name:
Pancreatic alpha-amylase (EC:3.2.1.11 Publication)
Short name:
PA
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolase
OrganismiStruthio camelus (Common ostrich)Curated
Taxonomic identifieri8801 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesPalaeognathaeStruthioniformesStruthionidaeStruthio

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000542911 – 497Pancreatic alpha-amylaseAdd BLAST497

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1Pyrrolidone carboxylic acid1 Publication1
Disulfide bondi28 ↔ 86By similarity
Disulfide bondi70 ↔ 115By similarity
Disulfide bondi141 ↔ 160By similarity
Disulfide bondi379 ↔ 385By similarity
Disulfide bondi451 ↔ 463By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

PRIDEiP83053

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP83053
SMRiP83053
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Phylogenomic databases

HOVERGENiHBG000061

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequencei

Sequence statusi: Complete.

P83053-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
QYNPNTQPGR TSIVHLFEWR WADIALECER YLAPYGFGGV QVSPPNENVI
60 70 80 90 100
ITNPYRPWWE RYQPVSYKLC TRSGNENEFR DMVTRCNNVG VRIYVDAVKN
110 120 130 140 150
HMCGSGAGSG THSTCGAYFN AGNRDSPAVP YSGWDFNDGK CRTGSGEIEN
160 170 180 190 200
YGDASQVRDC RLVGLLDLAL EKDYVRSTVA GYMNHLIDIG VAGFRLDAAK
210 220 230 240 250
HMWPGDIKAF LDKLHNLNTN WFSSGSRPFI YQEVIDLGGE PITSSQYFGN
260 270 280 290 300
HRVTEFKYGA KLGTVIRKWN GEKMAYLKNW GEGWGFVPSD RALVFVDNHD
310 320 330 340 350
NQRGHGAGGA SILTFWDARL YKMAVGFMLA HPYGFTRVMS SFRWPRHFEN
360 370 380 390 400
GKDVNDWYGP PSNSDGSTKE VTINADSTCG NDWVCEHRWR QIRNMVIFRN
410 420 430 440 450
VVDGEPFSNW WDNNSNQVAF GRGSKGFIVF NNDDWHMNVD LYTGLPAGTY
460 470 480 490
CDVISGQKEG SRCTGIQVYV SGNGKANFQI SNNAEDPFIA IHVGAKL
Length:497
Mass (Da):55,898
Last modified:October 1, 2001 - v1
Checksum:i62DB18FCD80BCA8B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti35Y → K AA sequence (Ref. 2) Curated1
Sequence conflicti50 – 52IIT → VFN AA sequence (Ref. 2) Curated3

Similar proteinsi

Cross-referencesi

3D structure databases

ProteinModelPortaliP83053
SMRiP83053
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH13 Glycoside Hydrolase Family 13

Proteomic databases

PRIDEiP83053

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG000061

Family and domain databases

Gene3Di2.60.40.1180, 1 hit
InterProiView protein in InterPro
IPR006048 A-amylase/branching_C
IPR031319 A-amylase_C
IPR006046 Alpha_amylase
IPR006047 Glyco_hydro_13_cat_dom
IPR013780 Glyco_hydro_b
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF00128 Alpha-amylase, 1 hit
PF02806 Alpha-amylase_C, 1 hit
PRINTSiPR00110 ALPHAAMYLASE
SMARTiView protein in SMART
SM00642 Aamy, 1 hit
SM00632 Aamy_C, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAMYP_STRCA
AccessioniPrimary (citable) accession number: P83053
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: October 1, 2001
Last modified: February 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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Main funding by: National Institutes of Health

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