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Protein

Mitochondrial import receptor subunit TOM20-3

Gene

TOM20-3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the translocation pore.1 Publication

Miscellaneous

There are four genes (TOM20-1, TOM20-2, TOM20-3 and TOM20-4) which encode mitochondrial import receptor subunits TOM20.
In mammals and fungi, the transmembrane domain is located at the N-terminus while it is located at the C-terminus in plants. The overall orientation of the protein in the membrane is therefore inverted.

GO - Molecular functioni

GO - Biological processi

  • protein import into mitochondrial outer membrane Source: InterPro
  • protein targeting to mitochondrion Source: TAIR

Keywordsi

Biological processProtein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import receptor subunit TOM20-3
Alternative name(s):
Translocase of outer membrane 20 kDa subunit 3
Gene namesi
Name:TOM20-3
Ordered Locus Names:At3g27080
ORF Names:MOJ10.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G27080
TAIRilocus:2092045 AT3G27080

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 174CytoplasmicSequence analysisAdd BLAST174
Transmembranei175 – 192HelicalSequence analysisAdd BLAST18
Topological domaini193 – 202Mitochondrial intermembraneSequence analysis10

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Triple mutants tom20-2-tom20-3-tom20-4 are vible but display a slightly delayed flowering time.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000515451 – 202Mitochondrial import receptor subunit TOM20-3Add BLAST202

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP82874

PTM databases

iPTMnetiP82874
SwissPalmiP82874

Expressioni

Tissue specificityi

Expressed in roots, flowers, young cotyledons and leaves.1 Publication

Gene expression databases

ExpressionAtlasiP82874 baseline and differential
GenevisibleiP82874 AT

Interactioni

Subunit structurei

Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 6 different proteins (TOM5, TOM6, TOM7, TOM20, TOM22/TOM9 and TOM40).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NAC089Q94F582EBI-2352074,EBI-2319707

Protein-protein interaction databases

BioGridi7656, 16 interactors
IntActiP82874, 15 interactors
STRINGi3702.AT3G27080.1

Structurei

Secondary structure

1202
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP82874
SMRiP82874
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP82874

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati38 – 74TPR 1Add BLAST37
Repeati86 – 119TPR 2Add BLAST34

Sequence similaritiesi

Belongs to the Tom20 family.Curated

Keywords - Domaini

Repeat, TPR repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IKS7 Eukaryota
ENOG4111JA8 LUCA
HOGENOMiHOG000005870
InParanoidiP82874
OMAiWCIGNAY
OrthoDBiEOG09360NVP
PhylomeDBiP82874

Family and domain databases

Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR010547 TOM20_imprt_rcpt
IPR011990 TPR-like_helical_dom_sf
PANTHERiPTHR32409 PTHR32409, 1 hit
SUPFAMiSSF48452 SSF48452, 1 hit

Sequencei

Sequence statusi: Complete.

P82874-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDTETEFDRI LLFEQIRQDA ENTYKSNPLD ADNLTRWGGV LLELSQFHSI
60 70 80 90 100
SDAKQMIQEA ITKFEEALLI DPKKDEAVWC IGNAYTSFAF LTPDETEAKH
110 120 130 140 150
NFDLATQFFQ QAVDEQPDNT HYLKSLEMTA KAPQLHAEAY KQGLGSQPMG
160 170 180 190 200
RVEAPAPPSS KAVKNKKSSD AKYDAMGWVI LAIGVVAWIS FAKANVPVSP

PR
Length:202
Mass (Da):22,565
Last modified:March 1, 2001 - v1
Checksum:iF86A54F827F3A6D1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti12L → F in AAM64598 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ296025 mRNA Translation: CAC14430.1
AB026649 Genomic DNA Translation: BAB01089.1
CP002686 Genomic DNA Translation: AEE77264.1
AY040027 mRNA Translation: AAK64184.1
AY079411 mRNA Translation: AAL85142.1
AY087037 mRNA Translation: AAM64598.1
RefSeqiNP_189344.1, NM_113622.4
UniGeneiAt.6114

Genome annotation databases

EnsemblPlantsiAT3G27080.1; AT3G27080.1; AT3G27080
GeneIDi822326
GrameneiAT3G27080.1; AT3G27080.1; AT3G27080
KEGGiath:AT3G27080

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ296025 mRNA Translation: CAC14430.1
AB026649 Genomic DNA Translation: BAB01089.1
CP002686 Genomic DNA Translation: AEE77264.1
AY040027 mRNA Translation: AAK64184.1
AY079411 mRNA Translation: AAL85142.1
AY087037 mRNA Translation: AAM64598.1
RefSeqiNP_189344.1, NM_113622.4
UniGeneiAt.6114

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZU2NMR-A1-145[»]
ProteinModelPortaliP82874
SMRiP82874
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi7656, 16 interactors
IntActiP82874, 15 interactors
STRINGi3702.AT3G27080.1

PTM databases

iPTMnetiP82874
SwissPalmiP82874

Proteomic databases

PaxDbiP82874

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G27080.1; AT3G27080.1; AT3G27080
GeneIDi822326
GrameneiAT3G27080.1; AT3G27080.1; AT3G27080
KEGGiath:AT3G27080

Organism-specific databases

AraportiAT3G27080
TAIRilocus:2092045 AT3G27080

Phylogenomic databases

eggNOGiENOG410IKS7 Eukaryota
ENOG4111JA8 LUCA
HOGENOMiHOG000005870
InParanoidiP82874
OMAiWCIGNAY
OrthoDBiEOG09360NVP
PhylomeDBiP82874

Miscellaneous databases

EvolutionaryTraceiP82874
PROiPR:P82874

Gene expression databases

ExpressionAtlasiP82874 baseline and differential
GenevisibleiP82874 AT

Family and domain databases

Gene3Di1.25.40.10, 1 hit
InterProiView protein in InterPro
IPR010547 TOM20_imprt_rcpt
IPR011990 TPR-like_helical_dom_sf
PANTHERiPTHR32409 PTHR32409, 1 hit
SUPFAMiSSF48452 SSF48452, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTO203_ARATH
AccessioniPrimary (citable) accession number: P82874
Secondary accession number(s): Q8LBR8, Q9LSC8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: March 1, 2001
Last modified: April 25, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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