UniProtKB - P82471 (GNAQ_RAT)
Guanine nucleotide-binding protein G(q) subunit alpha
Gnaq
Functioni
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. Regulates B-cell selection and survival and is required to prevent B-cell-dependent autoimmunity. Regulates chemotaxis of BM-derived neutrophils and dendritic cells (in vitro). Transduces FFAR4 signaling in response to long-chain fatty acids (LCFAs).
By similaritySites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 53 | MagnesiumBy similarity | 1 | |
Metal bindingi | 186 | MagnesiumBy similarity | 1 | |
Binding sitei | 331 | GTP; via amide nitrogenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 46 – 53 | GTPBy similarity | 8 | |
Nucleotide bindingi | 180 – 186 | GTPBy similarity | 7 | |
Nucleotide bindingi | 205 – 209 | GTPBy similarity | 5 | |
Nucleotide bindingi | 274 – 277 | GTPBy similarity | 4 |
GO - Molecular functioni
- alkylglycerophosphoethanolamine phosphodiesterase activity Source: MGI
- G-protein beta/gamma-subunit complex binding Source: GO_Central
- G protein-coupled receptor binding Source: GO_Central
- GTPase activator activity Source: RGD
- GTPase activity Source: GO_Central
- GTP binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- protein-containing complex binding Source: RGD
GO - Biological processi
- action potential Source: RGD
- activation of phospholipase C activity Source: RGD
- adenylate cyclase-activating G protein-coupled receptor signaling pathway Source: RGD
- adenylate cyclase-modulating G protein-coupled receptor signaling pathway Source: GO_Central
- cranial skeletal system development Source: RGD
- developmental pigmentation Source: RGD
- embryonic digit morphogenesis Source: RGD
- endothelin receptor signaling pathway Source: RGD
- forebrain neuron development Source: RGD
- glutamate receptor signaling pathway Source: RGD
- G protein-coupled receptor signaling pathway Source: RGD
- heart development Source: RGD
- maternal behavior Source: RGD
- multicellular organism aging Source: RGD
- negative regulation of apoptotic process Source: RGD
- negative regulation of potassium ion transport Source: RGD
- negative regulation of protein kinase activity Source: RGD
- neuron remodeling Source: RGD
- phospholipase C-activating dopamine receptor signaling pathway Source: RGD
- phospholipase C-activating G protein-coupled receptor signaling pathway Source: RGD
- positive regulation of smooth muscle cell proliferation Source: RGD
- post-embryonic development Source: RGD
- protein stabilization Source: RGD
- regulation of blood pressure Source: RGD
- regulation of canonical Wnt signaling pathway Source: RGD
- regulation of melanocyte differentiation Source: RGD
- skeletal system development Source: RGD
Keywordsi
Molecular function | Transducer |
Ligand | GTP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-RNO-112043, PLC beta mediated events R-RNO-202040, G-protein activation R-RNO-399997, Acetylcholine regulates insulin secretion R-RNO-416476, G alpha (q) signalling events R-RNO-418592, ADP signalling through P2Y purinoceptor 1 R-RNO-428930, Thromboxane signalling through TP receptor R-RNO-434316, Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion R-RNO-456926, Thrombin signalling through proteinase activated receptors (PARs) |
Names & Taxonomyi
Protein namesi | Recommended name: Guanine nucleotide-binding protein G(q) subunit alphaAlternative name(s): Guanine nucleotide-binding protein alpha-q |
Gene namesi | Name:Gnaq |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620770, Gnaq |
Subcellular locationi
Golgi apparatus
- Golgi apparatus By similarity
Nucleus
- Nucleus By similarity
- Nucleus membrane By similarity
Plasma membrane
- Cell membrane By similarity; Lipid-anchor By similarity
Note: Colocalizes with the adrenergic receptors, ADREN1A and ADREN1B, at the nuclear membrane of cardiac myocytes.By similarity
Cytosol
- cytosol Source: RGD
Golgi apparatus
- Golgi apparatus Source: UniProtKB-SubCell
Nucleus
- nuclear membrane Source: UniProtKB-SubCell
Plasma Membrane
- caveola Source: RGD
- heterotrimeric G-protein complex Source: MGI
- plasma membrane Source: RGD
Other locations
Keywords - Cellular componenti
Cell membrane, Golgi apparatus, Membrane, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000203762 | 1 – 359 | Guanine nucleotide-binding protein G(q) subunit alphaAdd BLAST | 359 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 9 | S-palmitoyl cysteineBy similarity | 1 | |
Lipidationi | 10 | S-palmitoyl cysteineBy similarity | 1 | |
Modified residuei | 209 | 5-glutamyl histamineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, PalmitateProteomic databases
jPOSTi | P82471 |
PaxDbi | P82471 |
PRIDEi | P82471 |
2D gel databases
World-2DPAGEi | 0004:P82471 |
PTM databases
iPTMneti | P82471 |
PhosphoSitePlusi | P82471 |
SwissPalmi | P82471 |
Interactioni
Subunit structurei
G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Binds SLC9A3R1.
Forms a complex with PECAM1 and BDKRB2.
Interacts with PECAM1 (By similarity).
Interacts with GAS2L2 (By similarity).
By similarityGO - Molecular functioni
- G protein-coupled receptor binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 249565, 4 interactors |
CORUMi | P82471 |
IntActi | P82471, 1 interactor |
MINTi | P82471 |
STRINGi | 10116.ENSRNOP00000019174 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 38 – 359 | G-alphaPROSITE-ProRule annotationAdd BLAST | 322 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 41 – 54 | G1 motifPROSITE-ProRule annotationAdd BLAST | 14 | |
Regioni | 178 – 186 | G2 motifPROSITE-ProRule annotation | 9 | |
Regioni | 201 – 210 | G3 motifPROSITE-ProRule annotation | 10 | |
Regioni | 270 – 277 | G4 motifPROSITE-ProRule annotation | 8 | |
Regioni | 329 – 334 | G5 motifPROSITE-ProRule annotation | 6 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0085, Eukaryota |
InParanoidi | P82471 |
OrthoDBi | 754573at2759 |
PhylomeDBi | P82471 |
Family and domain databases
CDDi | cd00066, G-alpha, 1 hit |
Gene3Di | 1.10.400.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR000654, Gprotein_alpha_Q IPR001019, Gprotein_alpha_su IPR011025, GproteinA_insert IPR027417, P-loop_NTPase |
PANTHERi | PTHR10218, PTHR10218, 1 hit |
Pfami | View protein in Pfam PF00503, G-alpha, 1 hit |
PRINTSi | PR00318, GPROTEINA PR00442, GPROTEINAQ |
SMARTi | View protein in SMART SM00275, G_alpha, 1 hit |
SUPFAMi | SSF47895, SSF47895, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51882, G_ALPHA, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MTLESIMACC LSEEAKEARR INDEIERQLR RDKRDARREL KLLLLGTGES
60 70 80 90 100
GKSTFIKQMR IIHGSGYSDE DKRGFTKLVY QNIFTAMQAM VRAMDTLKIP
110 120 130 140 150
YKYEHNKAHA QLVREVDVEK VSAFENPYVD AIKSLWNDPG IQECYDRRRE
160 170 180 190 200
YQLSDSTKYY LNDLDRVADP SYLPTQQDVL RVRVPTTGII EYPFDLQSVI
210 220 230 240 250
FRMVDVGGQR SERRKWIHCF ENVTSIMFLV ALSEYDQVLV ESDNENRMEE
260 270 280 290 300
SKALFRTIIT YPWFQNSSVI LFLNKKDLLE EKIMYSHLVD YFPEYDGPQR
310 320 330 340 350
DAQAAREFIL KMFVDLNPDS DKIIYSHFTC ATDTENIRFV FAAVKDTILQ
LNLKEYNLV
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD4AE68 | D4AE68_RAT | Guanine nucleotide-binding protein ... | Gnaq | 356 | Annotation score: | ||
A0A8I6AJ23 | A0A8I6AJ23_RAT | Guanine nucleotide binding protein,... | Gnaq rCG_47814 | 359 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 91 | V → I (PubMed:1333286).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF234260 mRNA Translation: AAF59930.1 L37294 mRNA Translation: AAB02848.1 |
RefSeqi | NP_112298.1, NM_031036.1 |
Genome annotation databases
GeneIDi | 81666 |
KEGGi | rno:81666 |
UCSCi | RGD:620770, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF234260 mRNA Translation: AAF59930.1 L37294 mRNA Translation: AAB02848.1 |
RefSeqi | NP_112298.1, NM_031036.1 |
3D structure databases
AlphaFoldDBi | P82471 |
SMRi | P82471 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 249565, 4 interactors |
CORUMi | P82471 |
IntActi | P82471, 1 interactor |
MINTi | P82471 |
STRINGi | 10116.ENSRNOP00000019174 |
PTM databases
iPTMneti | P82471 |
PhosphoSitePlusi | P82471 |
SwissPalmi | P82471 |
2D gel databases
World-2DPAGEi | 0004:P82471 |
Proteomic databases
jPOSTi | P82471 |
PaxDbi | P82471 |
PRIDEi | P82471 |
Genome annotation databases
GeneIDi | 81666 |
KEGGi | rno:81666 |
UCSCi | RGD:620770, rat |
Organism-specific databases
CTDi | 2776 |
RGDi | 620770, Gnaq |
Phylogenomic databases
eggNOGi | KOG0085, Eukaryota |
InParanoidi | P82471 |
OrthoDBi | 754573at2759 |
PhylomeDBi | P82471 |
Enzyme and pathway databases
Reactomei | R-RNO-112043, PLC beta mediated events R-RNO-202040, G-protein activation R-RNO-399997, Acetylcholine regulates insulin secretion R-RNO-416476, G alpha (q) signalling events R-RNO-418592, ADP signalling through P2Y purinoceptor 1 R-RNO-428930, Thromboxane signalling through TP receptor R-RNO-434316, Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion R-RNO-456926, Thrombin signalling through proteinase activated receptors (PARs) |
Miscellaneous databases
PROi | PR:P82471 |
Family and domain databases
CDDi | cd00066, G-alpha, 1 hit |
Gene3Di | 1.10.400.10, 1 hit 3.40.50.300, 1 hit |
InterProi | View protein in InterPro IPR000654, Gprotein_alpha_Q IPR001019, Gprotein_alpha_su IPR011025, GproteinA_insert IPR027417, P-loop_NTPase |
PANTHERi | PTHR10218, PTHR10218, 1 hit |
Pfami | View protein in Pfam PF00503, G-alpha, 1 hit |
PRINTSi | PR00318, GPROTEINA PR00442, GPROTEINAQ |
SMARTi | View protein in SMART SM00275, G_alpha, 1 hit |
SUPFAMi | SSF47895, SSF47895, 1 hit SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS51882, G_ALPHA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GNAQ_RAT | |
Accessioni | P82471Primary (citable) accession number: P82471 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | July 7, 2009 | |
Last modified: | May 25, 2022 | |
This is version 149 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families