UniProtKB - P81299 (CDC14_CAEEL)
Protein
Tyrosine-protein phosphatase cdc-14
Gene
cdc-14
Organism
Caenorhabditis elegans
Status
Functioni
Protein phosphatase that negatively regulates the G1-to-S phase transition to inhibit the cell cycle and establish quiescence in cells of multiple lineages including vulval, hypodermal and intestinal (PubMed:15247923, PubMed:21723944). Promotes nuclear accumulation and activity of the cyclin-dependent kinase inhibitor cki-1 which leads to inhibition of G1 progression during vulval tissue development (PubMed:15247923). Has been shown to not be required for cytokinesis (PubMed:15247923). However, in the embryo, in a contrasting study, has been shown to act as a regulator of central spindle formation and cytokinesis, and may be required for localization of the spindle component zen-4, and its interacting partner air-2 at the spindle during late cell divisions (PubMed:12213836).3 Publications
Main regulator of cell cycle arrest in vulval precursor cells.1 Publication
Caution
Has been shown in one report to not be localized to centrosomes or nuclei, and based on RNAi-mediated knockdown studies, has been shown to play a role in cytokinesis (PubMed:12213836). However, has also been reported to localize to centrosomes and nuclei and to have no role in cytokinesis, based on results obtained using the cdc-14 he118 mutant and RNAi-mediated knockdown (PubMed:15247923).2 Publications
Catalytic activityi
- H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] + phosphatePROSITE-ProRule annotation1 PublicationEC:3.1.3.48PROSITE-ProRule annotation1 Publication
Activity regulationi
Inhibited by sodium orthovanadate. Weakly inhibited by sodium fluoride and okadaic acid.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 295 | Phosphocysteine intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- phosphatase activity Source: WormBase
- phosphoprotein phosphatase activity Source: WormBase
- protein serine/threonine phosphatase activity Source: GO_Central
- protein tyrosine/serine/threonine phosphatase activity Source: InterPro
- protein tyrosine phosphatase activity Source: GO_Central
GO - Biological processi
- cilium assembly Source: GO_Central
- dephosphorylation Source: WormBase
- microtubule cytoskeleton organization Source: UniProtKB
- mitotic cell cycle Source: GO_Central
- mitotic cell cycle arrest Source: WormBase
- mitotic cytokinesis Source: WormBase
- mitotic spindle midzone assembly Source: WormBase
- negative regulation of cell cycle Source: UniProtKB
- negative regulation of cell cycle G1/S phase transition Source: UniProtKB
- polar body extrusion after meiotic divisions Source: WormBase
- positive regulation of cytokinesis Source: GO_Central
- protein dephosphorylation Source: WormBase
- regulation of cell development Source: UniProtKB
- regulation of exit from mitosis Source: GO_Central
- regulation of vulval development Source: UniProtKB
Keywordsi
Molecular function | Hydrolase, Protein phosphatase |
Biological process | Cell cycle, Cell division |
Enzyme and pathway databases
Reactomei | R-CEL-176407, Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase R-CEL-5687128, MAPK6/MAPK4 signaling |
Names & Taxonomyi
Protein namesi | Recommended name: Tyrosine-protein phosphatase cdc-14 (EC:3.1.3.48PROSITE-ProRule annotation1 Publication)Alternative name(s): Cell division cycle-related protein 14 |
Gene namesi | ORF Names:C17G10.4Imported |
Organismi | Caenorhabditis elegans |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | C17G10.4a ; CE30868 ; WBGene00000383 ; cdc-14 C17G10.4c ; CE08288 ; WBGene00000383 ; cdc-14 C17G10.4d ; CE06845 ; WBGene00000383 ; cdc-14 C17G10.4e ; CE36916 ; WBGene00000383 ; cdc-14 C17G10.4g ; CE50122 ; WBGene00000383 ; cdc-14 C17G10.4h ; CE01789 ; WBGene00000383 ; cdc-14 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 2 Publications
Nucleus
- Nucleus 1 Publication
Cytoskeleton
- centrosome 1 Publication
- spindle 2 Publications
Other locations
- Midbody 1 Publication
Note: Localizes to the cytoplasm throughout interphase (PubMed:15247923). As cells enter prophase, accumulates at the centrosomes (PubMed:15247923). During metaphase and anaphase, localizes to the spindle, in particular spindle asters and the spindle mid zone (PubMed:15247923, PubMed:12213836). During late telophase, localizes to cytoplasmic aggregates (PubMed:15247923). In some studies, localizes to the midbody during late telophase (PubMed:12213836). Localizes to the cytoplasm in G1-arrested cells (PubMed:15247923). Localizes to the nucleus or nucleolus in postmitotic cells (PubMed:15247923). Some studies report no localization to centrosomes or nuclei (PubMed:12213836). Co-localizes with zen-4 at the spindle, and localization may be dependent on each other (PubMed:12213836).2 Publications
Cytoskeleton
- astral microtubule Source: WormBase
- centrosome Source: WormBase
- kinociliary basal body Source: GO_Central
- mitotic spindle Source: GO_Central
- mitotic spindle midzone Source: WormBase
- spindle Source: UniProtKB
- spindle midzone Source: WormBase
- spindle pole Source: GO_Central
Nucleus
Other locations
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, Microtubule, NucleusPathology & Biotechi
Disruption phenotypei
RNAi-mediated knockdown results in extra cell divisions in the vulval lineage, but does not result in defects in cytokinesis (PubMed:15247923, PubMed:21723944). RNAi-mediated knockdown decreases cki-1 expression in the nucleus of vulval precursor cells (PubMed:15247923). In contrasting studies RNAi-mediated knockdown results in embryonic lethality with the production of multinucleated embryos that exhibit cytokinesis failure during telophase, no central spindle formation, and failed localization of the spindle component zen-4 and its interacting partner air-2 at the spindle in either anaphase or telophase (PubMed:12213836).3 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 295 | C → S: Abolishes phosphatase activity. 1 Publication | 1 | |
Mutagenesisi | 366 – 371 | KRNVRR → AANVAA: Disrupts nuclear localization. Localizes to the cytoplasm; when associated with 372-A--A-381. 1 Publication | 6 | |
Mutagenesisi | 372 – 381 | LVNQVDDINL → AVNQVDDANA: Impairs nuclear export signal. Disrupts cytoplasmic localization, conferring nuclear localization during interphase. Localizes to the cytoplasm; when associated with 366-A--A-371. 1 Publication | 10 | |
Mutagenesisi | 446 – 709 | Missing in he118; no effect on viability, embryogenesis or fertility. Disrupts cell cycle arrest of vulval precursor, intestinal, and hypodermal cells, and of precursor cells that give rise to male specific structures during postembryonic development. Vulval precursor cells undergo an extra round of cell division during the L2 larval stage of development, but the additional precursor cells do not disrupt formation of the vulval structure and animals undergo normal vulval morphogenesis during the L4 larval stage. Does not result in defects in cytokinesis. Overexpression of the cyclin dependent kinase inhibitor cki-1, or knockout with the G1/S-specific cyclin-E cye-1 (eh10), rescues the cell division phenotype. 1 PublicationAdd BLAST | 264 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000094880 | 1 – 709 | Tyrosine-protein phosphatase cdc-14Add BLAST | 709 |
Proteomic databases
EPDi | P81299 |
PaxDbi | P81299 |
PeptideAtlasi | P81299 |
PTM databases
iPTMneti | P81299 |
Expressioni
Developmental stagei
Expressed in the soma during early embryogenesis, and widely expressed after hatching. Isoform C: Expressed in the soma during early embryogenesis, but after hatching, expression in larvae is restricted to V and P lineages, the postembryonic lineages that contribute to the seam cells and vulval precursor cells, respectively.1 Publication
Gene expression databases
Bgeei | WBGene00000383, Expressed in multi-cellular organism and 5 other tissues |
Interactioni
Protein-protein interaction databases
BioGRIDi | 39287, 7 interactors |
DIPi | DIP-25903N |
STRINGi | 6239.C17G10.4b.2 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 196 – 354 | Tyrosine-protein phosphatasePROSITE-ProRule annotationAdd BLAST | 159 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 366 – 371 | Nuclear localization signal1 Publication | 6 | |
Motifi | 372 – 381 | Nuclear export signal1 Publication | 10 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1720, Eukaryota |
GeneTreei | ENSGT00940000170847 |
HOGENOMi | CLU_288890_0_0_1 |
InParanoidi | P81299 |
OMAi | CANGKSY |
OrthoDBi | 1357618at2759 |
Family and domain databases
CDDi | cd14499, CDC14_C, 1 hit cd17657, CDC14_N, 1 hit |
Gene3Di | 3.90.190.10, 2 hits |
InterProi | View protein in InterPro IPR026070, CDC14 IPR044506, CDC14_C IPR029260, DSPn IPR029021, Prot-tyrosine_phosphatase-like IPR016130, Tyr_Pase_AS IPR000387, TYR_PHOSPHATASE_dom IPR020422, TYR_PHOSPHATASE_DUAL_dom |
PANTHERi | PTHR23339:SF27, PTHR23339:SF27, 1 hit |
Pfami | View protein in Pfam PF14671, DSPn, 1 hit |
SMARTi | View protein in SMART SM00195, DSPc, 1 hit |
SUPFAMi | SSF52799, SSF52799, 2 hits |
PROSITEi | View protein in PROSITE PS00383, TYR_PHOSPHATASE_1, 1 hit PS50056, TYR_PHOSPHATASE_2, 1 hit PS50054, TYR_PHOSPHATASE_DUAL, 1 hit |
s (6)i Sequence
Sequence statusi: Complete.
This entry describes 6 produced by isoformsialternative splicing. AlignAdd to basketIsoform hImported (identifier: P81299-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MREDHSPRRN NTIENTLTEL LPNRLYFGCF PNPDAIDKSD KSVKKTCFIN
60 70 80 90 100
INNKFHYEPF YEDFGPWNLS VLYRLCVQVG KLLEVEEKRS RRVVLFCQDD
110 120 130 140 150
GTGQYDKIRV NTAYVLGAYL IIYQGFSADD AYLKVSSGET VKFVGFRDAS
160 170 180 190 200
MGSPQYLLHL HDVLRGIEKA LKFGWLDFSD FDYEEYEFYE RVENGDFNWI
210 220 230 240 250
IPGKILSFCG PHNESREENG YPYHAPDVYF DYFRENKVST IVRLNAKNYD
260 270 280 290 300
ASKFTKAGFD HVDLFFIDGS TPSDEIMLKF IKVVDNTKGG VAVHCKAGLG
310 320 330 340 350
RTGTLIACWM MKEYGLTAGE CMGWLRVCRP GSVIGPQQPY LIEKQKFCWS
360 370 380 390 400
LSQSNGVHLT QNKEEKRNVR RLVNQVDDIN LGEERISPKS RENTRPNILR
410 420 430 440 450
RRVQVQNGRS TAPVTIAPAG TSESRRSTKP SRVVDETALD DQGRSQGDRL
460 470 480 490 500
LQLKAKHQHE SETTSPNSSS SRRFVKSSTP QMTVPSQAYL NRNREPIIVT
510 520 530 540 550
PSKNGTSSGT SSRQLKTTPN GNVAYRTRNS SGNTTSTLTR TGNESYRFHN
560 570 580 590 600
SLFYEPASAV FPSMASRRSE TTRYLSPTTP IKPMSPSYTD GTSPRYKARL
610 620 630 640 650
RSENPIGSTT STPFSLQPQI TKCSLTAESK PPKRILSMPG TSKSTSSLKK
660 670 680 690 700
IQVSRPRPYP STGVRVELCA NGKSYDIRPR KEAHVIPGAG LAANTEALLG
VCKLVNTLS
Sequence cautioni
The sequence AAT74543 differs from that shown.Curated
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_059807 | 542 – 555 | Missing in isoform a, isoform c and isoform d. CuratedAdd BLAST | 14 | |
Alternative sequenceiVSP_059808 | 620 – 709 | ITKCS…VNTLS → FGLVRVPPDSPHSIMAHRPP PTTSSRAPLSPHNYSTTQGY STSSRGLYGDKKPLARGSVS TSTLPSMYMTRSCERK in isoform c. CuratedAdd BLAST | 90 | |
Alternative sequenceiVSP_059809 | 620 – 695 | ITKCS…LAANT → FGLVRVPPDSPHSIMAHRPP PTTSSRAPLSPHNYSTTQGY STSSRGLYGDKKPLARGSVS TSTLPSMYMTRSCERK in isoform g. CuratedAdd BLAST | 76 | |
Alternative sequenceiVSP_059810 | 696 – 709 | Missing in isoform g. CuratedAdd BLAST | 14 | |
Alternative sequenceiVSP_059811 | 701 – 708 | VCKLVNTL → KRRKTA in isoform a and isoform e. Curated | 8 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY661743 mRNA Translation: AAT74542.1 AY661744 mRNA Translation: AAT74543.1 Sequence problems. AY661745 mRNA Translation: AAT74544.1 AY661746 mRNA Translation: AAT74545.1 AY661747 mRNA Translation: AAT74546.1 AF000363 mRNA Translation: AAB94407.1 BX284602 Genomic DNA Translation: CCD64951.1 BX284602 Genomic DNA Translation: CCD64953.1 BX284602 Genomic DNA Translation: CCD64954.1 BX284602 Genomic DNA Translation: CCD64955.1 BX284602 Genomic DNA Translation: CTQ86448.1 BX284602 Genomic DNA Translation: CTQ86449.1 |
PIRi | E88158 T34097 |
RefSeqi | NP_001021969.1, NM_001026798.5 [P81299-5] NP_001300509.1, NM_001313580.1 NP_001300510.1, NM_001313581.1 [P81299-1] NP_495085.2, NM_062684.5 NP_495086.1, NM_062685.3 NP_740991.1, NM_170993.4 |
Genome annotation databases
EnsemblMetazoai | C17G10.4a.1; C17G10.4a.1; WBGene00000383 [P81299-2] C17G10.4a.2; C17G10.4a.2; WBGene00000383 [P81299-2] C17G10.4c.1; C17G10.4c.1; WBGene00000383 [P81299-3] C17G10.4c.2; C17G10.4c.2; WBGene00000383 [P81299-3] C17G10.4d.1; C17G10.4d.1; WBGene00000383 [P81299-4] C17G10.4e.1; C17G10.4e.1; WBGene00000383 [P81299-5] C17G10.4e.2; C17G10.4e.2; WBGene00000383 [P81299-5] C17G10.4g.1; C17G10.4g.1; WBGene00000383 [P81299-7] C17G10.4h.1; C17G10.4h.1; WBGene00000383 [P81299-1] |
GeneIDi | 173945 |
UCSCi | C17G10.4b, c. elegans [P81299-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY661743 mRNA Translation: AAT74542.1 AY661744 mRNA Translation: AAT74543.1 Sequence problems. AY661745 mRNA Translation: AAT74544.1 AY661746 mRNA Translation: AAT74545.1 AY661747 mRNA Translation: AAT74546.1 AF000363 mRNA Translation: AAB94407.1 BX284602 Genomic DNA Translation: CCD64951.1 BX284602 Genomic DNA Translation: CCD64953.1 BX284602 Genomic DNA Translation: CCD64954.1 BX284602 Genomic DNA Translation: CCD64955.1 BX284602 Genomic DNA Translation: CTQ86448.1 BX284602 Genomic DNA Translation: CTQ86449.1 |
PIRi | E88158 T34097 |
RefSeqi | NP_001021969.1, NM_001026798.5 [P81299-5] NP_001300509.1, NM_001313580.1 NP_001300510.1, NM_001313581.1 [P81299-1] NP_495085.2, NM_062684.5 NP_495086.1, NM_062685.3 NP_740991.1, NM_170993.4 |
3D structure databases
SMRi | P81299 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 39287, 7 interactors |
DIPi | DIP-25903N |
STRINGi | 6239.C17G10.4b.2 |
PTM databases
iPTMneti | P81299 |
Proteomic databases
EPDi | P81299 |
PaxDbi | P81299 |
PeptideAtlasi | P81299 |
Genome annotation databases
EnsemblMetazoai | C17G10.4a.1; C17G10.4a.1; WBGene00000383 [P81299-2] C17G10.4a.2; C17G10.4a.2; WBGene00000383 [P81299-2] C17G10.4c.1; C17G10.4c.1; WBGene00000383 [P81299-3] C17G10.4c.2; C17G10.4c.2; WBGene00000383 [P81299-3] C17G10.4d.1; C17G10.4d.1; WBGene00000383 [P81299-4] C17G10.4e.1; C17G10.4e.1; WBGene00000383 [P81299-5] C17G10.4e.2; C17G10.4e.2; WBGene00000383 [P81299-5] C17G10.4g.1; C17G10.4g.1; WBGene00000383 [P81299-7] C17G10.4h.1; C17G10.4h.1; WBGene00000383 [P81299-1] |
GeneIDi | 173945 |
UCSCi | C17G10.4b, c. elegans [P81299-1] |
Organism-specific databases
CTDi | 173945 |
WormBasei | C17G10.4a ; CE30868 ; WBGene00000383 ; cdc-14 C17G10.4c ; CE08288 ; WBGene00000383 ; cdc-14 C17G10.4d ; CE06845 ; WBGene00000383 ; cdc-14 C17G10.4e ; CE36916 ; WBGene00000383 ; cdc-14 C17G10.4g ; CE50122 ; WBGene00000383 ; cdc-14 C17G10.4h ; CE01789 ; WBGene00000383 ; cdc-14 |
Phylogenomic databases
eggNOGi | KOG1720, Eukaryota |
GeneTreei | ENSGT00940000170847 |
HOGENOMi | CLU_288890_0_0_1 |
InParanoidi | P81299 |
OMAi | CANGKSY |
OrthoDBi | 1357618at2759 |
Enzyme and pathway databases
Reactomei | R-CEL-176407, Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase R-CEL-5687128, MAPK6/MAPK4 signaling |
Miscellaneous databases
PROi | PR:P81299 |
Gene expression databases
Bgeei | WBGene00000383, Expressed in multi-cellular organism and 5 other tissues |
Family and domain databases
CDDi | cd14499, CDC14_C, 1 hit cd17657, CDC14_N, 1 hit |
Gene3Di | 3.90.190.10, 2 hits |
InterProi | View protein in InterPro IPR026070, CDC14 IPR044506, CDC14_C IPR029260, DSPn IPR029021, Prot-tyrosine_phosphatase-like IPR016130, Tyr_Pase_AS IPR000387, TYR_PHOSPHATASE_dom IPR020422, TYR_PHOSPHATASE_DUAL_dom |
PANTHERi | PTHR23339:SF27, PTHR23339:SF27, 1 hit |
Pfami | View protein in Pfam PF14671, DSPn, 1 hit |
SMARTi | View protein in SMART SM00195, DSPc, 1 hit |
SUPFAMi | SSF52799, SSF52799, 2 hits |
PROSITEi | View protein in PROSITE PS00383, TYR_PHOSPHATASE_1, 1 hit PS50056, TYR_PHOSPHATASE_2, 1 hit PS50054, TYR_PHOSPHATASE_DUAL, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CDC14_CAEEL | |
Accessioni | P81299Primary (citable) accession number: P81299 Secondary accession number(s): A0A0K3AQY3 Q8MQD6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 30, 2003 |
Last sequence update: | September 12, 2018 | |
Last modified: | April 7, 2021 | |
This is version 161 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase - SIMILARITY comments
Index of protein domains and families