UniProtKB - P81274 (GPSM2_HUMAN)
G-protein-signaling modulator 2
GPSM2
Functioni
Miscellaneous
Caution
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 608 | GDP; shared with dimeric partnerBy similarity | 1 | |
Binding sitei | 613 | GDP; shared with dimeric partnerBy similarity | 1 | |
Binding sitei | 642 | GDP; shared with dimeric partnerBy similarity | 1 | |
Binding sitei | 647 | GDP; shared with dimeric partnerBy similarity | 1 |
GO - Molecular functioni
- dynein complex binding Source: UniProtKB
- GDP-dissociation inhibitor activity Source: UniProtKB
- identical protein binding Source: IntAct
- nucleotide binding Source: UniProtKB-KW
- protein C-terminus binding Source: UniProtKB
- protein domain specific binding Source: UniProtKB
- protein self-association Source: UniProtKB
GO - Biological processi
- cell division Source: UniProtKB-KW
- establishment of mitotic spindle orientation Source: UniProtKB
- G protein-coupled receptor signaling pathway Source: ProtInc
- maintenance of centrosome location Source: UniProtKB
- mitotic spindle organization Source: UniProtKB
- positive regulation of protein localization to cell cortex Source: UniProtKB
- positive regulation of spindle assembly Source: UniProtKB
- Ran protein signal transduction Source: UniProtKB
- regulation of mitotic spindle organization Source: UniProtKB
Keywordsi
Biological process | Cell cycle, Cell division, Mitosis, Transport |
Ligand | Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | P81274 |
Reactomei | R-HSA-418594, G alpha (i) signalling events |
SIGNORi | P81274 |
Names & Taxonomyi
Protein namesi | Recommended name: G-protein-signaling modulator 2Alternative name(s): Mosaic protein LGN1 Publication |
Gene namesi | Name:GPSM2 Synonyms:LGN |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:29501, GPSM2 |
MIMi | 609245, gene |
neXtProti | NX_P81274 |
VEuPathDBi | HostDB:ENSG00000121957.12 |
Subcellular locationi
Plasma membrane
- Lateral cell membrane 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 3 Publications
Cytoskeleton
- spindle pole 2 Publications
Other locations
- cell cortex 4 Publications
Note: Localizes in the cytoplasm during interphase and at cell cortex during metaphase (PubMed:11781568, PubMed:15632202, PubMed:22074847). Colocalizes with NUMA1 to mitotic spindle poles (PubMed:11781568, PubMed:21816348). Localized at the central and lateral cell cortex regions in a RanGTP-dependent manner (PubMed:22327364). In horizontally retinal progenitor dividing cells, localized to the lateral cortical region. In vertically retinal progenitor dividing cells, localized at the polar cortical region (By similarity).By similarity5 Publications
Cytoskeleton
- centrosome Source: HPA
- mitotic spindle pole Source: UniProtKB
Cytosol
- cytosol Source: HPA
Plasma Membrane
- lateral plasma membrane Source: UniProtKB-SubCell
Other locations
- cell cortex Source: UniProtKB
- cell cortex region Source: UniProtKB
- cytoplasm Source: UniProtKB
- lateral cell cortex Source: UniProtKB
- protein-containing complex Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Cytoskeleton, MembranePathology & Biotechi
Involvement in diseasei
Chudley-McCullough syndrome (CMCS)2 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 228 | R → A: Abolishes location at mitotic spindle poles; when associated with A-243. 1 Publication | 1 | |
Mutagenesisi | 228 | R → E: Strongly reduces interaction with INSC. Abolishes interaction with INSC; when associated with R-290. 1 Publication | 1 | |
Mutagenesisi | 243 | R → A: Abolishes location at mitotic spindle poles; when associated with A-228. 1 Publication | 1 | |
Mutagenesisi | 290 | N → R: Abolishes interaction with INSC; when associated with E-228. 1 Publication | 1 |
Keywords - Diseasei
DeafnessOrganism-specific databases
DisGeNETi | 29899 |
MalaCardsi | GPSM2 |
MIMi | 604213, phenotype |
OpenTargetsi | ENSG00000121957 |
Orphaneti | 90636, Autosomal recessive non-syndromic sensorineural deafness type DFNB 314597, Chudley-McCullough syndrome |
PharmGKBi | PA134993615 |
Miscellaneous databases
Pharosi | P81274, Tbio |
Genetic variation databases
BioMutai | GPSM2 |
DMDMi | 294862507 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000106358 | 1 – 684 | G-protein-signaling modulator 2Add BLAST | 684 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 132 | Phosphoserine; by PKGSequence analysis | 1 | |
Modified residuei | 352 | Phosphoserine; by PKGSequence analysis | 1 | |
Modified residuei | 408 | PhosphoserineCombined sources | 1 | |
Modified residuei | 483 | PhosphoserineCombined sources | 1 | |
Modified residuei | 486 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 501 | Phosphoserine; by PKCSequence analysis | 1 | |
Modified residuei | 541 | PhosphoserineCombined sources | 1 | |
Modified residuei | 565 | PhosphoserineCombined sources | 1 | |
Modified residuei | 607 | Phosphoserine; by PKGSequence analysis | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P81274 |
jPOSTi | P81274 |
MassIVEi | P81274 |
MaxQBi | P81274 |
PaxDbi | P81274 |
PeptideAtlasi | P81274 |
PRIDEi | P81274 |
ProteomicsDBi | 57693 |
PTM databases
iPTMneti | P81274 |
PhosphoSitePlusi | P81274 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000121957, Expressed in tibia and 236 other tissues |
ExpressionAtlasi | P81274, baseline and differential |
Genevisiblei | P81274, HS |
Organism-specific databases
HPAi | ENSG00000121957, Tissue enhanced (skin) |
Interactioni
Subunit structurei
Interacts with the dynein-dynactin complex; this interaction is inhibited in a PLK1-dependent manner (PubMed:22327364).
Part of a spindle orientation complex at least composed of GNAI1, GPSM2 and NUMA1 (PubMed:26766442).
Interacts with LLGL2 (PubMed:15632202).
Interacts (via TPR repeat region) with INSC/inscuteable (PubMed:16458856, PubMed:22074847).
Interacts (via TPR repeat region) with NUMA1 (via C-terminus); this interaction is direct, inhibited in a PLK1-dependent manner, prevents the binding of NUMA1 with SPAG5 and promotes spindle pole organization (PubMed:11781568, PubMed:22327364, PubMed:27462074). INSC and NUMA1 compete for the same binding site, but INSC has higher affinity and can displace NUMA1 (in vitro) (PubMed:22074847).
Interacts with GNAI2 (PubMed:8973305).
Interacts (via GoLoco domains) with the GDP-bound form of GNAI1 and GNAI3; has much lower affinity for the GTP-bound form. Interaction with GDP-bound GNAI3 strongly enhances the affinity for NUMA1 (By similarity).
Interacts (via TPR repeat region) with FRMPD1 (PubMed:22074847). INSC and FRMPD1 compete for the same binding site, but INSC has higher affinity and can displace FRMPD1 (in vitro) (By similarity).
Interacts (via TPR repeat region) with FRMPD4 (PubMed:22074847, PubMed:25664792).
Identified in a complex with INSC and F2RL2/Par3 (PubMed:16458856).
Interacts with TASOR (By similarity).
By similarity9 PublicationsBinary interactionsi
Hide detailsP81274
GO - Molecular functioni
- identical protein binding Source: IntAct
- protein C-terminus binding Source: UniProtKB
- protein domain specific binding Source: UniProtKB
- protein self-association Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 118949, 21 interactors |
CORUMi | P81274 |
DIPi | DIP-399N |
IntActi | P81274, 21 interactors |
MINTi | P81274 |
STRINGi | 9606.ENSP00000385510 |
Miscellaneous databases
RNActi | P81274, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P81274 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 24 – 57 | TPR 1Add BLAST | 34 | |
Repeati | 62 – 95 | TPR 2Add BLAST | 34 | |
Repeati | 102 – 135 | TPR 3Add BLAST | 34 | |
Repeati | 142 – 184 | TPR 4Add BLAST | 43 | |
Repeati | 202 – 235 | TPR 5Add BLAST | 34 | |
Repeati | 242 – 275 | TPR 6Add BLAST | 34 | |
Repeati | 282 – 315 | TPR 7Add BLAST | 34 | |
Repeati | 322 – 355 | TPR 8Add BLAST | 34 | |
Domaini | 489 – 511 | GoLoco 1PROSITE-ProRule annotationAdd BLAST | 23 | |
Domaini | 544 – 566 | GoLoco 2PROSITE-ProRule annotationAdd BLAST | 23 | |
Domaini | 594 – 616 | GoLoco 3PROSITE-ProRule annotationAdd BLAST | 23 | |
Domaini | 628 – 650 | GoLoco 4PROSITE-ProRule annotationAdd BLAST | 23 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 22 – 357 | Important for interaction with NUMA1; INSC and FRMPD1By similarityAdd BLAST | 336 |
Sequence similaritiesi
Keywords - Domaini
Repeat, TPR repeatPhylogenomic databases
eggNOGi | KOG1130, Eukaryota |
GeneTreei | ENSGT00940000161257 |
HOGENOMi | CLU_012445_1_0_1 |
InParanoidi | P81274 |
OMAi | AKLHFVN |
OrthoDBi | 733786at2759 |
PhylomeDBi | P81274 |
TreeFami | TF328344 |
Family and domain databases
Gene3Di | 1.25.40.10, 3 hits |
IDEALi | IID00313 |
InterProi | View protein in InterPro IPR003109, GoLoco_motif IPR013026, TPR-contain_dom IPR011990, TPR-like_helical_dom_sf IPR019734, TPR_repeat |
Pfami | View protein in Pfam PF02188, GoLoco, 4 hits PF13176, TPR_7, 2 hits PF13181, TPR_8, 1 hit |
SMARTi | View protein in SMART SM00390, GoLoco, 4 hits SM00028, TPR, 7 hits |
SUPFAMi | SSF48452, SSF48452, 2 hits |
PROSITEi | View protein in PROSITE PS50877, GOLOCO, 4 hits PS50005, TPR, 6 hits PS50293, TPR_REGION, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 13 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MEENLISMRE DHSFHVRYRM EASCLELALE GERLCKSGDC RAGVSFFEAA
60 70 80 90 100
VQVGTEDLKT LSAIYSQLGN AYFYLHDYAK ALEYHHHDLT LARTIGDQLG
110 120 130 140 150
EAKASGNLGN TLKVLGNFDE AIVCCQRHLD ISRELNDKVG EARALYNLGN
160 170 180 190 200
VYHAKGKSFG CPGPQDVGEF PEEVRDALQA AVDFYEENLS LVTALGDRAA
210 220 230 240 250
QGRAFGNLGN THYLLGNFRD AVIAHEQRLL IAKEFGDKAA ERRAYSNLGN
260 270 280 290 300
AYIFLGEFET ASEYYKKTLL LARQLKDRAV EAQSCYSLGN TYTLLQDYEK
310 320 330 340 350
AIDYHLKHLA IAQELNDRIG EGRACWSLGN AYTALGNHDQ AMHFAEKHLE
360 370 380 390 400
ISREVGDKSG ELTARLNLSD LQMVLGLSYS TNNSIMSENT EIDSSLNGVR
410 420 430 440 450
PKLGRRHSME NMELMKLTPE KVQNWNSEIL AKQKPLIAKP SAKLLFVNRL
460 470 480 490 500
KGKKYKTNSS TKVLQDASNS IDHRIPNSQR KISADTIGDE GFFDLLSRFQ
510 520 530 540 550
SNRMDDQRCC LQEKNCHTAS TTTSSTPPKM MLKTSSVPVV SPNTDEFLDL
560 570 580 590 600
LASSQSRRLD DQRASFSNLP GLRLTQNSQS VLSHLMTNDN KEADEDFFDI
610 620 630 640 650
LVKCQGSRLD DQRCAPPPAT TKGPTVPDED FFSLILRSQG KRMDEQRVLL
660 670 680
QRDQNRDTDF GLKDFLQNNA LLEFKNSGKK SADH
Computationally mapped potential isoform sequencesi
There are 13 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH0Y4A4 | H0Y4A4_HUMAN | G-protein-signaling modulator 2 | GPSM2 | 627 | Annotation score: | ||
A0A6Q8PF02 | A0A6Q8PF02_HUMAN | G-protein-signaling modulator 2 | GPSM2 | 701 | Annotation score: | ||
A0A6Q8PFD2 | A0A6Q8PFD2_HUMAN | G-protein-signaling modulator 2 | GPSM2 | 519 | Annotation score: | ||
A0A6Q8PGW7 | A0A6Q8PGW7_HUMAN | G-protein-signaling modulator 2 | GPSM2 | 625 | Annotation score: | ||
Q5T1N9 | Q5T1N9_HUMAN | G-protein-signaling modulator 2 | GPSM2 | 213 | Annotation score: | ||
A0A6Q8PGS6 | A0A6Q8PGS6_HUMAN | G-protein-signaling modulator 2 | GPSM2 | 384 | Annotation score: | ||
A0A6Q8PGU2 | A0A6Q8PGU2_HUMAN | G-protein-signaling modulator 2 | GPSM2 | 465 | Annotation score: | ||
A0A2R8Y6E3 | A0A2R8Y6E3_HUMAN | G-protein-signaling modulator 2 | GPSM2 | 311 | Annotation score: | ||
B0QZD0 | B0QZD0_HUMAN | G-protein-signaling modulator 2 | GPSM2 | 75 | Annotation score: | ||
B0QZC9 | B0QZC9_HUMAN | G-protein-signaling modulator 2 | GPSM2 | 52 | Annotation score: | ||
There are more potential isoformsShow all |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 400 | R → L in AAB40385 (PubMed:8973305).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U54999 mRNA Translation: AAB40385.1 Different initiation. AB445462 mRNA Translation: BAH84760.1 AL449266 Genomic DNA No translation available. CH471122 Genomic DNA Translation: EAW56340.1 BC027732 mRNA Translation: AAH27732.1 Different initiation. AY136740 mRNA Translation: AAN01266.1 CR456786 mRNA Translation: CAG33067.1 |
CCDSi | CCDS792.2 |
PIRi | JC5334, G02540 |
RefSeqi | NP_001307967.1, NM_001321038.1 NP_001307968.1, NM_001321039.1 NP_037428.3, NM_013296.4 XP_011539603.1, XM_011541301.2 XP_011539604.1, XM_011541302.2 XP_016856586.1, XM_017001097.1 XP_016856587.1, XM_017001098.1 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U54999 mRNA Translation: AAB40385.1 Different initiation. AB445462 mRNA Translation: BAH84760.1 AL449266 Genomic DNA No translation available. CH471122 Genomic DNA Translation: EAW56340.1 BC027732 mRNA Translation: AAH27732.1 Different initiation. AY136740 mRNA Translation: AAN01266.1 CR456786 mRNA Translation: CAG33067.1 |
CCDSi | CCDS792.2 |
PIRi | JC5334, G02540 |
RefSeqi | NP_001307967.1, NM_001321038.1 NP_001307968.1, NM_001321039.1 NP_037428.3, NM_013296.4 XP_011539603.1, XM_011541301.2 XP_011539604.1, XM_011541302.2 XP_016856586.1, XM_017001097.1 XP_016856587.1, XM_017001098.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3SF4 | X-ray | 2.60 | A/B/C | 20-421 | [»] | |
4WND | X-ray | 1.50 | A | 20-421 | [»] | |
4WNE | X-ray | 2.00 | A | 20-421 | [»] | |
4WNF | X-ray | 2.90 | A | 20-421 | [»] | |
4WNG | X-ray | 2.11 | A | 20-421 | [»] | |
5A6C | X-ray | 2.90 | A/B | 22-357 | [»] | |
6HC2 | X-ray | 4.31 | A/C/E/G/I/K/M/O/Q/S/U/W | 14-374 | [»] | |
SMRi | P81274 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 118949, 21 interactors |
CORUMi | P81274 |
DIPi | DIP-399N |
IntActi | P81274, 21 interactors |
MINTi | P81274 |
STRINGi | 9606.ENSP00000385510 |
PTM databases
iPTMneti | P81274 |
PhosphoSitePlusi | P81274 |
Genetic variation databases
BioMutai | GPSM2 |
DMDMi | 294862507 |
Proteomic databases
EPDi | P81274 |
jPOSTi | P81274 |
MassIVEi | P81274 |
MaxQBi | P81274 |
PaxDbi | P81274 |
PeptideAtlasi | P81274 |
PRIDEi | P81274 |
ProteomicsDBi | 57693 |
Protocols and materials databases
Antibodypediai | 1983, 196 antibodies |
DNASUi | 29899 |
Genome annotation databases
Organism-specific databases
CTDi | 29899 |
DisGeNETi | 29899 |
GeneCardsi | GPSM2 |
HGNCi | HGNC:29501, GPSM2 |
HPAi | ENSG00000121957, Tissue enhanced (skin) |
MalaCardsi | GPSM2 |
MIMi | 604213, phenotype 609245, gene |
neXtProti | NX_P81274 |
OpenTargetsi | ENSG00000121957 |
Orphaneti | 90636, Autosomal recessive non-syndromic sensorineural deafness type DFNB 314597, Chudley-McCullough syndrome |
PharmGKBi | PA134993615 |
VEuPathDBi | HostDB:ENSG00000121957.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1130, Eukaryota |
GeneTreei | ENSGT00940000161257 |
HOGENOMi | CLU_012445_1_0_1 |
InParanoidi | P81274 |
OMAi | AKLHFVN |
OrthoDBi | 733786at2759 |
PhylomeDBi | P81274 |
TreeFami | TF328344 |
Enzyme and pathway databases
PathwayCommonsi | P81274 |
Reactomei | R-HSA-418594, G alpha (i) signalling events |
SIGNORi | P81274 |
Miscellaneous databases
BioGRID-ORCSi | 29899, 7 hits in 1002 CRISPR screens |
ChiTaRSi | GPSM2, human |
GeneWikii | GPSM2 |
GenomeRNAii | 29899 |
Pharosi | P81274, Tbio |
PROi | PR:P81274 |
RNActi | P81274, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000121957, Expressed in tibia and 236 other tissues |
ExpressionAtlasi | P81274, baseline and differential |
Genevisiblei | P81274, HS |
Family and domain databases
Gene3Di | 1.25.40.10, 3 hits |
IDEALi | IID00313 |
InterProi | View protein in InterPro IPR003109, GoLoco_motif IPR013026, TPR-contain_dom IPR011990, TPR-like_helical_dom_sf IPR019734, TPR_repeat |
Pfami | View protein in Pfam PF02188, GoLoco, 4 hits PF13176, TPR_7, 2 hits PF13181, TPR_8, 1 hit |
SMARTi | View protein in SMART SM00390, GoLoco, 4 hits SM00028, TPR, 7 hits |
SUPFAMi | SSF48452, SSF48452, 2 hits |
PROSITEi | View protein in PROSITE PS50877, GOLOCO, 4 hits PS50005, TPR, 6 hits PS50293, TPR_REGION, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GPSM2_HUMAN | |
Accessioni | P81274Primary (citable) accession number: P81274 Secondary accession number(s): Q5T1N8, Q6IBL7, Q8N0Z5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | April 20, 2010 | |
Last modified: | April 7, 2021 | |
This is version 209 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families