UniProtKB - P81187 (CFAB_BOVIN)
Complement factor B
CFB
Functioni
Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase.
Catalytic activityi
- Cleavage of Arg-|-Ser bond in complement component C3 alpha-chain to yield C3a and C3b, and Arg-|-Xaa bond in complement component C5 alpha-chain to yield C5a and C5b. EC:3.4.21.47
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 526 | Charge relay systemBy similarity | 1 | |
Active sitei | 576 | Charge relay systemBy similarity | 1 | |
Active sitei | 699 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- serine-type endopeptidase activity Source: UniProtKB-EC
GO - Biological processi
- complement activation, alternative pathway Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Protease, Serine protease |
Biological process | Complement alternate pathway, Immunity, Innate immunity |
Protein family/group databases
MEROPSi | S01.196 |
Names & Taxonomyi
Protein namesi | Recommended name: Complement factor B (EC:3.4.21.47)Alternative name(s): C3/C5 convertase EC-VMFB Cleaved into the following 2 chains: |
Gene namesi | Name:CFB Synonyms:BF |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000046158 |
VGNCi | VGNC:107285, CFB |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 25 | By similarityAdd BLAST | 25 | |
ChainiPRO_0000285857 | 26 – 761 | Complement factor BAdd BLAST | 736 | |
ChainiPRO_0000285858 | 26 – 259 | Complement factor B Ba fragmentAdd BLAST | 234 | |
ChainiPRO_0000027541 | 260 – 761 | Complement factor B Bb fragmentAdd BLAST | 502 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 37 ↔ 76 | By similarity | ||
Disulfide bondi | 62 ↔ 98 | By similarity | ||
Disulfide bondi | 103 ↔ 145 | By similarity | ||
Glycosylationi | 122 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 131 ↔ 158 | By similarity | ||
Glycosylationi | 142 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 165 ↔ 205 | By similarity | ||
Disulfide bondi | 191 ↔ 218 | By similarity | ||
Glycosylationi | 285 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 378 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 511 ↔ 527 | By similarity | ||
Disulfide bondi | 695 ↔ 725 | By similarity |
Keywords - PTMi
Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, ZymogenProteomic databases
PaxDbi | P81187 |
PeptideAtlasi | P81187 |
PRIDEi | P81187 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000046158, Expressed in liver and 94 other tissues |
ExpressionAtlasi | P81187, baseline and differential |
Interactioni
Subunit structurei
Monomer (By similarity). Part of the C3-convertase enzyme complex comprised of Complement C3 beta chain (C3b) and Complement factor B Bb fragment (Bb) and CFP (By similarity). Interacts to C3b; this interaction is dependent on the presence of Mg2+ (By similarity). Interacts to CFP; this interaction contributes to the stabilization of the active C3-convertase enzyme complex (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 9913.ENSBTAP00000009800 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 35 – 100 | Sushi 1PROSITE-ProRule annotationAdd BLAST | 66 | |
Domaini | 101 – 160 | Sushi 2PROSITE-ProRule annotationAdd BLAST | 60 | |
Domaini | 163 – 220 | Sushi 3PROSITE-ProRule annotationAdd BLAST | 58 | |
Domaini | 270 – 469 | VWFAPROSITE-ProRule annotationAdd BLAST | 200 | |
Domaini | 477 – 754 | Peptidase S1PROSITE-ProRule annotationAdd BLAST | 278 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Repeat, Signal, SushiPhylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00940000158605 |
HOGENOMi | CLU_022004_1_0_1 |
InParanoidi | P81187 |
OMAi | QKGHENC |
OrthoDBi | 172139at2759 |
TreeFami | TF330194 |
Family and domain databases
CDDi | cd00033, CCP, 3 hits cd00190, Tryp_SPc, 1 hit |
Gene3Di | 3.40.50.410, 1 hit |
InterProi | View protein in InterPro IPR011360, Compl_C2_B IPR028341, Complement_B IPR009003, Peptidase_S1_PA IPR001314, Peptidase_S1A IPR035976, Sushi/SCR/CCP_sf IPR000436, Sushi_SCR_CCP_dom IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER IPR002035, VWF_A IPR036465, vWFA_dom_sf |
PANTHERi | PTHR46393, PTHR46393, 1 hit PTHR46393:SF1, PTHR46393:SF1, 1 hit |
Pfami | View protein in Pfam PF00084, Sushi, 2 hits PF00089, Trypsin, 1 hit PF00092, VWA, 1 hit |
PIRSFi | PIRSF001154, Compl_C2_B, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00032, CCP, 3 hits SM00020, Tryp_SPc, 1 hit SM00327, VWA, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF53300, SSF53300, 1 hit SSF57535, SSF57535, 3 hits |
PROSITEi | View protein in PROSITE PS50923, SUSHI, 3 hits PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit PS50234, VWFA, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MGIGHNPRLC LVPLILGLLC GGVGMTPLPE AGPQSPCSLE GVEIKGGSFR
60 70 80 90 100
LLKAGQVLEY LCPSGFYPYP TQIRTCRSTG SWSTLQTQDR KIVKRAECKA
110 120 130 140 150
IRCPRPQDFE NGEYWPRAAY YNLSDEISFR CYDGYTLRGS ANRTCQGNGR
160 170 180 190 200
WDGETAICDD GATYCPNPGI PLGTRKVGSQ YRLEDRVTYY CNRGLTLRGS
210 220 230 240 250
EQRTCLEGGS WSGTEPSCQD SFMYDTPAEV AEAFLSSLTE TIEGVDAEDG
260 270 280 290 300
HSPGEQQKRK IVLDPSGSMN IYLVLDGSDS VGAHNFTGAK NCLRDFIEKV
310 320 330 340 350
ASYGVKPKYG LVTYATEPKV LIRVSDPKSS EADWVTDQLN QINYADHKLK
360 370 380 390 400
AGTNTKRALL EVYNMMSREV NQFKETWNRT RHVIIIMTDG LHNMGGDPVT
410 420 430 440 450
VIHDIRYLLD IGRNRKNPRE DYLDIYVFGV GPLVNQENIN ALASKKDKEK
460 470 480 490 500
HVFKLQGMEN LEDVFVQMLD ESRTLGLCGM VWEHKDGTAY HKQPWQAKIS
510 520 530 540 550
VTRPSKGHES CMGAIVSEYF VLTAAHCFTV DDEKHSIKVS LGGQRKEWEV
560 570 580 590 600
KEILFHPKYD LNAKKAKGIP EFYDYDVALV RLKEKLKYET TIRPICLPCT
610 620 630 640 650
EGSIQALRLP RSTTCQQQMQ ELLPAKDIEA LFVSESKKTL TRKAVYIKNG
660 670 680 690 700
DKKASCERDA LRAPGYEKVK DVSEVVTPRF LCTGGVDPYA DPNTCKGDSG
710 720 730 740 750
GPLIIHKRSR FIQVGVISWG VVDVCKRPQQ VPGYARDFHI NLYQVLPWLK
760
EKLQNEDLGF L
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A3Q1LRP5 | A0A3Q1LRP5_BOVIN | Complement factor B | CFB | 725 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC112504 mRNA Translation: AAI12505.1 |
RefSeqi | NP_001035616.1, NM_001040526.1 |
Genome annotation databases
Ensembli | ENSBTAT00000009800; ENSBTAP00000009800; ENSBTAG00000046158 |
GeneIDi | 514076 |
KEGGi | bta:514076 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC112504 mRNA Translation: AAI12505.1 |
RefSeqi | NP_001035616.1, NM_001040526.1 |
3D structure databases
AlphaFoldDBi | P81187 |
SMRi | P81187 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9913.ENSBTAP00000009800 |
Protein family/group databases
MEROPSi | S01.196 |
Proteomic databases
PaxDbi | P81187 |
PeptideAtlasi | P81187 |
PRIDEi | P81187 |
Genome annotation databases
Ensembli | ENSBTAT00000009800; ENSBTAP00000009800; ENSBTAG00000046158 |
GeneIDi | 514076 |
KEGGi | bta:514076 |
Organism-specific databases
CTDi | 629 |
VEuPathDBi | HostDB:ENSBTAG00000046158 |
VGNCi | VGNC:107285, CFB |
Phylogenomic databases
eggNOGi | KOG3627, Eukaryota |
GeneTreei | ENSGT00940000158605 |
HOGENOMi | CLU_022004_1_0_1 |
InParanoidi | P81187 |
OMAi | QKGHENC |
OrthoDBi | 172139at2759 |
TreeFami | TF330194 |
Gene expression databases
Bgeei | ENSBTAG00000046158, Expressed in liver and 94 other tissues |
ExpressionAtlasi | P81187, baseline and differential |
Family and domain databases
CDDi | cd00033, CCP, 3 hits cd00190, Tryp_SPc, 1 hit |
Gene3Di | 3.40.50.410, 1 hit |
InterProi | View protein in InterPro IPR011360, Compl_C2_B IPR028341, Complement_B IPR009003, Peptidase_S1_PA IPR001314, Peptidase_S1A IPR035976, Sushi/SCR/CCP_sf IPR000436, Sushi_SCR_CCP_dom IPR001254, Trypsin_dom IPR018114, TRYPSIN_HIS IPR033116, TRYPSIN_SER IPR002035, VWF_A IPR036465, vWFA_dom_sf |
PANTHERi | PTHR46393, PTHR46393, 1 hit PTHR46393:SF1, PTHR46393:SF1, 1 hit |
Pfami | View protein in Pfam PF00084, Sushi, 2 hits PF00089, Trypsin, 1 hit PF00092, VWA, 1 hit |
PIRSFi | PIRSF001154, Compl_C2_B, 1 hit |
PRINTSi | PR00722, CHYMOTRYPSIN |
SMARTi | View protein in SMART SM00032, CCP, 3 hits SM00020, Tryp_SPc, 1 hit SM00327, VWA, 1 hit |
SUPFAMi | SSF50494, SSF50494, 1 hit SSF53300, SSF53300, 1 hit SSF57535, SSF57535, 3 hits |
PROSITEi | View protein in PROSITE PS50923, SUSHI, 3 hits PS50240, TRYPSIN_DOM, 1 hit PS00134, TRYPSIN_HIS, 1 hit PS00135, TRYPSIN_SER, 1 hit PS50234, VWFA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CFAB_BOVIN | |
Accessioni | P81187Primary (citable) accession number: P81187 Secondary accession number(s): Q2KIU6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | May 1, 2007 | |
Last modified: | May 25, 2022 | |
This is version 128 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families