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UniProtKB - P80966 (PA2A1_OPHHA)
Protein
Acidic phospholipase A2 1
Gene
N/A
Organism
Ophiophagus hannah (King cobra) (Naja hannah)
Status
Functioni
Snake venom phospholipase A2 (PLA2) that may exhibit cardiotoxicity, myotoxicity, antiplatelet activity, and edema-inducing activity. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
1 PublicationCatalytic activityi
- a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1-acyl-sn-glycero-3-phosphocholine + a fatty acid + H+PROSITE-ProRule annotationEC:3.1.1.4PROSITE-ProRule annotation
Cofactori
Ca2+1 PublicationNote: Binds 1 Ca2+ ion.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 55 | Calcium; via carbonyl oxygenCombined sources1 Publication | 1 | |
Metal bindingi | 57 | Calcium; via carbonyl oxygenCombined sources1 Publication | 1 | |
Metal bindingi | 59 | Calcium; via carbonyl oxygenCombined sources1 Publication | 1 | |
Active sitei | 75 | By similarity | 1 | |
Metal bindingi | 76 | CalciumCombined sources1 Publication | 1 | |
Active sitei | 126 | By similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- phospholipase A2 activity Source: UniProtKB-EC
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- arachidonic acid secretion Source: InterPro
- lipid catabolic process Source: UniProtKB-KW
- phospholipid metabolic process Source: InterPro
Keywordsi
Molecular function | Cardiotoxin, Hemostasis impairing toxin, Hydrolase, Myotoxin, Platelet aggregation inhibiting toxin, Toxin |
Biological process | Lipid degradation, Lipid metabolism |
Ligand | Calcium, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Acidic phospholipase A2 1 (EC:3.1.1.4)Short name: svPLA2 Alternative name(s): APLA2-1 OHV A-PLA2 Short name: OHV-APLA2 Phosphatidylcholine 2-acylhydrolase |
Organismi | Ophiophagus hannah (King cobra) (Naja hannah) |
Taxonomic identifieri | 8665 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Bifurcata › Unidentata › Episquamata › Toxicofera › Serpentes › Colubroidea › Elapidae › Elapinae › Ophiophagus |
Subcellular locationi
Extracellular region or secreted
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 21 | Sequence analysisAdd BLAST | 21 | |
PropeptideiPRO_0000022942 | 22 – 27 | 1 Publication | 6 | |
ChainiPRO_0000022943 | 28 – 151 | Acidic phospholipase A2 1Add BLAST | 124 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 38 ↔ 104 | Combined sources1 Publication | ||
Disulfide bondi | 54 ↔ 151 | Combined sources1 Publication | ||
Disulfide bondi | 56 ↔ 72 | Combined sources1 Publication | ||
Disulfide bondi | 71 ↔ 132 | Combined sources1 Publication | ||
Disulfide bondi | 78 ↔ 125 | Combined sources1 Publication | ||
Disulfide bondi | 88 ↔ 118 | Combined sources1 Publication | ||
Disulfide bondi | 111 ↔ 123 | Combined sources1 Publication |
Keywords - PTMi
Disulfide bondExpressioni
Tissue specificityi
Expressed by the venom gland.
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P80966 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P80966 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
SignalFamily and domain databases
CDDi | cd00125, PLA2c, 1 hit |
Gene3Di | 1.20.90.10, 1 hit |
InterProi | View protein in InterPro IPR001211, PLipase_A2 IPR033112, PLipase_A2_Asp_AS IPR016090, PLipase_A2_dom IPR036444, PLipase_A2_dom_sf IPR033113, PLipase_A2_His_AS |
PANTHERi | PTHR11716, PTHR11716, 1 hit |
Pfami | View protein in Pfam PF00068, Phospholip_A2_1, 1 hit |
PRINTSi | PR00389, PHPHLIPASEA2 |
SMARTi | View protein in SMART SM00085, PA2c, 1 hit |
SUPFAMi | SSF48619, SSF48619, 1 hit |
PROSITEi | View protein in PROSITE PS00119, PA2_ASP, 1 hit PS00118, PA2_HIS, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P80966-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNPAHLLVLS AVCVSLLGAS SIPPQPLHLI QFGNMIQCTV PGFLSWIKYA
60 70 80 90 100
DYGCYCGAGG SGTPVDKLDR CCQVHDNCYT QAQKLPACSS IMDSPYVKIY
110 120 130 140 150
SYDCSERTVT CKADNDECAA FICNCDRVAA HCFAASPYNN NNYNIDTTTR
C
Mass spectrometryi
Molecular mass is 13720.47 Da. Determined by ESI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF302908 mRNA Translation: AAG23964.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF302908 mRNA Translation: AAG23964.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1GP7 | X-ray | 2.60 | A/B/C | 1-151 | [»] | |
SMRi | P80966 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P80966 |
Family and domain databases
CDDi | cd00125, PLA2c, 1 hit |
Gene3Di | 1.20.90.10, 1 hit |
InterProi | View protein in InterPro IPR001211, PLipase_A2 IPR033112, PLipase_A2_Asp_AS IPR016090, PLipase_A2_dom IPR036444, PLipase_A2_dom_sf IPR033113, PLipase_A2_His_AS |
PANTHERi | PTHR11716, PTHR11716, 1 hit |
Pfami | View protein in Pfam PF00068, Phospholip_A2_1, 1 hit |
PRINTSi | PR00389, PHPHLIPASEA2 |
SMARTi | View protein in SMART SM00085, PA2c, 1 hit |
SUPFAMi | SSF48619, SSF48619, 1 hit |
PROSITEi | View protein in PROSITE PS00119, PA2_ASP, 1 hit PS00118, PA2_HIS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PA2A1_OPHHA | |
Accessioni | P80966Primary (citable) accession number: P80966 Secondary accession number(s): Q9DF32 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | August 30, 2002 | |
Last modified: | February 23, 2022 | |
This is version 114 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Animal Toxin Annotation Program | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families