Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pyruvate kinase

Gene

pyk

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • K+By similarity

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 1 (gapA)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)
  4. Enolase (eno)
  5. Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei32SubstrateBy similarity1
Metal bindingi34PotassiumBy similarity1
Metal bindingi36PotassiumBy similarity1
Metal bindingi66PotassiumBy similarity1
Metal bindingi67Potassium; via carbonyl oxygenBy similarity1
Sitei220Transition state stabilizerBy similarity1
Metal bindingi222MagnesiumBy similarity1
Binding sitei245Substrate; via amide nitrogenBy similarity1
Metal bindingi246MagnesiumBy similarity1
Binding sitei246Substrate; via amide nitrogenBy similarity1
Binding sitei278SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

BioCyciBSUB:BSU29180-MONOMER
UniPathwayiUPA00109; UER00188

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Alternative name(s):
Vegetative protein 17
Short name:
VEG17
Gene namesi
Name:pyk
Synonyms:pykA
Ordered Locus Names:BSU29180
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001120561 – 585Pyruvate kinaseAdd BLAST585

Proteomic databases

PaxDbiP80885
PRIDEiP80885

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

IntActiP80885, 1 interactor
MINTiP80885
STRINGi224308.Bsubs1_010100015921

Structurei

3D structure databases

ProteinModelPortaliP80885
SMRiP80885
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated
In the C-terminal section; belongs to the PEP-utilizing enzyme family.Curated

Phylogenomic databases

eggNOGiENOG4105CA9 Bacteria
COG0469 LUCA
COG3848 LUCA
HOGENOMiHOG000021559
InParanoidiP80885
KOiK00873
OMAiKHEAIEQ
PhylomeDBiP80885

Family and domain databases

CDDicd00288 Pyruvate_Kinase, 1 hit
Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 1 hit
InterProiView protein in InterPro
IPR008279 PEP-util_enz_mobile_dom
IPR036637 Phosphohistidine_dom_sf
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR015795 Pyrv_Knase_C
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00391 PEP-utilizers, 1 hit
PF00224 PK, 1 hit
PF02887 PK_C, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52009 SSF52009, 1 hit
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

P80885-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKTKIVCTI GPASESIEML TKLMESGMNV ARLNFSHGDF EEHGARIKNI
60 70 80 90 100
REASKKLGKN VGILLDTKGP EIRTHTMENG GIELETGKEL IISMDEVVGT
110 120 130 140 150
TDKISVTYEG LVHDVEQGST ILLDDGLIGL EVLDVDAAKR EIKTKVLNNG
160 170 180 190 200
TLKNKKGVNV PGVSVNLPGI TEKDARDIVF GIEQGVDFIA PSFIRRSTDV
210 220 230 240 250
LEIRELLEEH NAQDIQIIPK IENQEGVDNI DAILEVSDGL MVARGDLGVE
260 270 280 290 300
IPAEEVPLVQ KELIKKCNAL GKPVITATQM LDSMQRNPRP TRAEASDVAN
310 320 330 340 350
AIFDGTDAIM LSGETAAGSY PVEAVQTMHN IASRSEEALN YKEILSKRRD
360 370 380 390 400
QVGMTITDAI GQSVAHTAIN LNAAAIVTPT ESGHTARMIA KYRPQAPIVA
410 420 430 440 450
VTVNDSISRK LALVSGVFAE SGQNASSTDE MLEDAVQKSL NSGIVKHGDL
460 470 480 490 500
IVITAGTVGE SGTTNLMKVH TVGDIIAKGQ GIGRKSAYGP VVVAQNAKEA
510 520 530 540 550
EQKMTDGAVL VTKSTDRDMI ASLEKASALI TEEGGLTSHA AVVGLSLGIP
560 570 580
VIVGLENATS ILTDGQDITV DASRGAVYQG RASVL
Length:585
Mass (Da):62,174
Last modified:July 15, 1998 - v2
Checksum:i3FA56DA5B52BE62F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF008220 Genomic DNA Translation: AAC00343.1
AL009126 Genomic DNA Translation: CAB14878.1
PIRiG69685
RefSeqiNP_390796.1, NC_000964.3
WP_004398560.1, NZ_JNCM01000036.1

Genome annotation databases

EnsemblBacteriaiCAB14878; CAB14878; BSU29180
GeneIDi936596
KEGGibsu:BSU29180
PATRICifig|224308.179.peg.3168

Similar proteinsi

Entry informationi

Entry nameiKPYK_BACSU
AccessioniPrimary (citable) accession number: P80885
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 15, 1998
Last modified: February 28, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health