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Protein

Succinate--CoA ligase [ADP-forming] subunit alpha

Gene

sucD

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.UniRule annotation

Catalytic activityi

ATP + succinate + CoA = ADP + phosphate + succinyl-CoA.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes succinate from succinyl-CoA (ligase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Succinate--CoA ligase [ADP-forming] subunit beta (sucC), Succinate--CoA ligase [ADP-forming] subunit alpha (sucD)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes succinate from succinyl-CoA (ligase route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43Coenzyme AUniRule annotation1
Binding sitei159Substrate; shared with subunit betaUniRule annotation1
Active sitei247Tele-phosphohistidine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processTricarboxylic acid cycle
LigandNucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU16100-MONOMER
UniPathwayi
UPA00223;UER00999

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate--CoA ligase [ADP-forming] subunit alphaUniRule annotation (EC:6.2.1.5UniRule annotation)
Alternative name(s):
Succinyl-CoA synthetase subunit alphaUniRule annotation
Short name:
SCS-alphaUniRule annotation
Gene namesi
Name:sucDUniRule annotation
Ordered Locus Names:BSU16100
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: GO_Central

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001027892 – 300Succinate--CoA ligase [ADP-forming] subunit alphaAdd BLAST299

Proteomic databases

PaxDbiP80865
PRIDEiP80865

Interactioni

Subunit structurei

Heterotetramer of two alpha and two beta subunits.UniRule annotation

Protein-protein interaction databases

IntActiP80865, 1 interactor
MINTiP80865
STRINGi224308.Bsubs1_010100008871

Structurei

3D structure databases

ProteinModelPortaliP80865
SMRiP80865
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni17 – 20Coenzyme A bindingUniRule annotation4
Regioni96 – 98Coenzyme A bindingUniRule annotation3

Sequence similaritiesi

Belongs to the succinate/malate CoA ligase alpha subunit family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CH8 Bacteria
COG0074 LUCA
HOGENOMiHOG000239685
InParanoidiP80865
KOiK01902
OMAiIIFVPPA
PhylomeDBiP80865

Family and domain databases

Gene3Di3.40.50.261, 1 hit
HAMAPiMF_01988 Succ_CoA_alpha, 1 hit
InterProiView protein in InterPro
IPR017440 Cit_synth/succinyl-CoA_lig_AS
IPR033847 Citrt_syn/SCS-alpha_CS
IPR003781 CoA-bd
IPR005810 CoA_lig_alpha
IPR005811 CoA_ligase
IPR036291 NAD(P)-bd_dom_sf
IPR016102 Succinyl-CoA_synth-like
PfamiView protein in Pfam
PF02629 CoA_binding, 1 hit
PF00549 Ligase_CoA, 1 hit
PIRSFiPIRSF001553 SucCS_alpha, 1 hit
SMARTiView protein in SMART
SM00881 CoA_binding, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF52210 SSF52210, 1 hit
TIGRFAMsiTIGR01019 sucCoAalpha, 1 hit
PROSITEiView protein in PROSITE
PS01216 SUCCINYL_COA_LIG_1, 1 hit
PS00399 SUCCINYL_COA_LIG_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80865-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSVFINKDTR VIVQGITGST ALFHTKQMLE YGTNIVGGVT PGKGGTEAEG
60 70 80 90 100
VPVFNTVAEA VQTTGANASV IYVPAPFAAD AIMEAVDAEL DLVICITEHI
110 120 130 140 150
PVLDMVKVKR FMEGKKTRLI GPNCPGVITP EECKIGIMPG YIHKKGHVGV
160 170 180 190 200
VSRSGTLTYE AVHQLSEAGV GQSTAVGIGG DPVNGTNFID VLKAFNEDPD
210 220 230 240 250
THAVIMIGEI GGTAEEEAAE WVKANMTKPV VGFIGGKTAP PGKRMGHAGA
260 270 280 290 300
IISGGKGTAD EKIKTLNACG IEVAETPSVM GETLIKVLKE KNLFETCKTH
Length:300
Mass (Da):31,382
Last modified:January 23, 2007 - v3
Checksum:iEE0462D35F2B9978
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000975 Genomic DNA Translation: CAA04420.1
AL009126 Genomic DNA Translation: CAB13483.1
PIRiF69719
RefSeqiNP_389492.1, NC_000964.3
WP_003238566.1, NZ_JNCM01000035.1

Genome annotation databases

EnsemblBacteriaiCAB13483; CAB13483; BSU16100
GeneIDi937797
KEGGibsu:BSU16100
PATRICifig|224308.179.peg.1750

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000975 Genomic DNA Translation: CAA04420.1
AL009126 Genomic DNA Translation: CAB13483.1
PIRiF69719
RefSeqiNP_389492.1, NC_000964.3
WP_003238566.1, NZ_JNCM01000035.1

3D structure databases

ProteinModelPortaliP80865
SMRiP80865
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP80865, 1 interactor
MINTiP80865
STRINGi224308.Bsubs1_010100008871

Proteomic databases

PaxDbiP80865
PRIDEiP80865

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13483; CAB13483; BSU16100
GeneIDi937797
KEGGibsu:BSU16100
PATRICifig|224308.179.peg.1750

Phylogenomic databases

eggNOGiENOG4105CH8 Bacteria
COG0074 LUCA
HOGENOMiHOG000239685
InParanoidiP80865
KOiK01902
OMAiIIFVPPA
PhylomeDBiP80865

Enzyme and pathway databases

UniPathwayi
UPA00223;UER00999

BioCyciBSUB:BSU16100-MONOMER

Miscellaneous databases

PROiPR:P80865

Family and domain databases

Gene3Di3.40.50.261, 1 hit
HAMAPiMF_01988 Succ_CoA_alpha, 1 hit
InterProiView protein in InterPro
IPR017440 Cit_synth/succinyl-CoA_lig_AS
IPR033847 Citrt_syn/SCS-alpha_CS
IPR003781 CoA-bd
IPR005810 CoA_lig_alpha
IPR005811 CoA_ligase
IPR036291 NAD(P)-bd_dom_sf
IPR016102 Succinyl-CoA_synth-like
PfamiView protein in Pfam
PF02629 CoA_binding, 1 hit
PF00549 Ligase_CoA, 1 hit
PIRSFiPIRSF001553 SucCS_alpha, 1 hit
SMARTiView protein in SMART
SM00881 CoA_binding, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF52210 SSF52210, 1 hit
TIGRFAMsiTIGR01019 sucCoAalpha, 1 hit
PROSITEiView protein in PROSITE
PS01216 SUCCINYL_COA_LIG_1, 1 hit
PS00399 SUCCINYL_COA_LIG_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSUCD_BACSU
AccessioniPrimary (citable) accession number: P80865
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 134 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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