UniProtKB - P80725 (DPS_LISIN)
Protein
DNA protection during starvation protein
Gene
dps
Organism
Listeria innocua serovar 6a (strain ATCC BAA-680 / CLIP 11262)
Status
Functioni
Protects DNA from oxidative damage by sequestering intracellular Fe2+ ion and storing it in the form of Fe3+ oxyhydroxide mineral. One hydrogen peroxide oxidizes two Fe2+ ions, which prevents hydroxyl radical production by the Fenton reaction. Does not bind DNA.3 Publications
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 31 | Iron 1; shared with dodecameric partner1 Publication | 1 | |
Metal bindingi | 58 | Iron 11 Publication | 1 | |
Metal bindingi | 62 | Iron 11 Publication | 1 | |
Metal bindingi | 62 | Iron 21 Publication | 1 |
GO - Molecular functioni
- ferric iron binding Source: InterPro
- oxidoreductase activity, oxidizing metal ions Source: InterPro
GO - Biological processi
- cellular iron ion homeostasis Source: UniProtKB-KW
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Iron storage |
Ligand | Iron, Metal-binding |
Enzyme and pathway databases
BRENDAi | 1.16.3.1, 3044 |
Names & Taxonomyi
Protein namesi | Recommended name: DNA protection during starvation protein (EC:1.16.-.-)Alternative name(s): Ferritin-like protein Non-heme iron-containing ferritin |
Gene namesi | Name:dps Synonyms:flp, fri Ordered Locus Names:lin0942 |
Organismi | Listeria innocua serovar 6a (strain ATCC BAA-680 / CLIP 11262) |
Taxonomic identifieri | 272626 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Bacillales › Listeriaceae › Listeria › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 31 | H → G: Slight decrease in DNA protection and significant decrease in iron affinity. Retains only one third of wild-type DNA protection and loses iron binding ability; when associated with G-43. 1 Publication | 1 | |
Mutagenesisi | 43 | H → G: Slight decrease in DNA protection and significant decrease iron affinity. Retains only one third of wild-type DNA protection and loses iron-binding ability; when associated with G-31. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000201657 | 1 – 156 | DNA protection during starvation proteinAdd BLAST | 156 |
Expressioni
Inductioni
By iron limitation and stationary growth phase.1 Publication
Interactioni
Subunit structurei
Homododecamer. The 12 subunits form a hollow sphere into which the mineral iron core of up to 500 Fe3+ can be deposited.
1 PublicationProtein-protein interaction databases
STRINGi | 272626.lin0942 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P80725 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P80725 |
Family & Domainsi
Domaini
12 di-nuclear ferroxidase centers are located at the interfaces between subunits related by 2-fold symmetry axes.
Sequence similaritiesi
Belongs to the Dps family.Curated
Phylogenomic databases
eggNOGi | COG0783, Bacteria |
HOGENOMi | CLU_098183_4_0_9 |
OMAi | DDYSIGR |
OrthoDBi | 1742631at2 |
Family and domain databases
CDDi | cd01043, DPS, 1 hit |
Gene3Di | 1.20.1260.10, 1 hit |
InterProi | View protein in InterPro IPR002177, DPS_DNA-bd IPR023188, DPS_DNA-bd_CS IPR012347, Ferritin-like IPR009078, Ferritin-like_SF IPR008331, Ferritin_DPS_dom |
PANTHERi | PTHR42932, PTHR42932, 1 hit |
Pfami | View protein in Pfam PF00210, Ferritin, 1 hit |
PIRSFi | PIRSF005900, Dps, 1 hit |
PRINTSi | PR01346, HELNAPAPROT |
SUPFAMi | SSF47240, SSF47240, 1 hit |
PROSITEi | View protein in PROSITE PS00818, DPS_1, 1 hit PS00819, DPS_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P80725-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKTINSVDTK EFLNHQVANL NVFTVKIHQI HWYMRGHNFF TLHEKMDDLY
60 70 80 90 100
SEFGEQMDEV AERLLAIGGS PFSTLKEFLE NASVEEAPYT KPKTMDQLME
110 120 130 140 150
DLVGTLELLR DEYKQGIELT DKEGDDVTND MLIAFKASID KHIWMFKAFL
GKAPLE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 63 | R → L (PubMed:12383509).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ244014 Genomic DNA Translation: CAB65175.2 AL596167 Genomic DNA Translation: CAC96173.1 |
PIRi | AE1550 |
RefSeqi | WP_003761404.1, NC_003212.1 |
Genome annotation databases
EnsemblBacteriai | CAC96173; CAC96173; CAC96173 |
GeneIDi | 57122566 |
KEGGi | lin:fri |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ244014 Genomic DNA Translation: CAB65175.2 AL596167 Genomic DNA Translation: CAC96173.1 |
PIRi | AE1550 |
RefSeqi | WP_003761404.1, NC_003212.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1QGH | X-ray | 2.35 | A/B/C/D/E/F/G/H/I/J/K/L | 1-156 | [»] | |
2BJY | X-ray | 2.60 | A/B/C/D/E/F/G/H/I/J/K/L | 1-156 | [»] | |
2BK6 | X-ray | 2.19 | A/B/C/D/E/F | 1-156 | [»] | |
2BKC | X-ray | 2.30 | A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/X/Y | 1-156 | [»] | |
6HUI | X-ray | 3.00 | A/B/C/D/E/F | 1-156 | [»] | |
6HV1 | X-ray | 2.55 | A/B/C/D/E/F | 1-156 | [»] | |
6HVQ | X-ray | 1.90 | A/B/C/D/E/F | 1-156 | [»] | |
6HX2 | X-ray | 1.60 | A/B/C/D/E/F | 1-156 | [»] | |
6SEV | X-ray | 2.00 | A/B/C/D/E/F | 7-156 | [»] | |
SMRi | P80725 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 272626.lin0942 |
Genome annotation databases
EnsemblBacteriai | CAC96173; CAC96173; CAC96173 |
GeneIDi | 57122566 |
KEGGi | lin:fri |
Phylogenomic databases
eggNOGi | COG0783, Bacteria |
HOGENOMi | CLU_098183_4_0_9 |
OMAi | DDYSIGR |
OrthoDBi | 1742631at2 |
Enzyme and pathway databases
BRENDAi | 1.16.3.1, 3044 |
Miscellaneous databases
EvolutionaryTracei | P80725 |
Family and domain databases
CDDi | cd01043, DPS, 1 hit |
Gene3Di | 1.20.1260.10, 1 hit |
InterProi | View protein in InterPro IPR002177, DPS_DNA-bd IPR023188, DPS_DNA-bd_CS IPR012347, Ferritin-like IPR009078, Ferritin-like_SF IPR008331, Ferritin_DPS_dom |
PANTHERi | PTHR42932, PTHR42932, 1 hit |
Pfami | View protein in Pfam PF00210, Ferritin, 1 hit |
PIRSFi | PIRSF005900, Dps, 1 hit |
PRINTSi | PR01346, HELNAPAPROT |
SUPFAMi | SSF47240, SSF47240, 1 hit |
PROSITEi | View protein in PROSITE PS00818, DPS_1, 1 hit PS00819, DPS_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DPS_LISIN | |
Accessioni | P80725Primary (citable) accession number: P80725 Secondary accession number(s): Q9RE06 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | November 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families