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Protein

Chymotrypsin B

Gene
N/A
Organism
Gadus morhua (Atlantic cod)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei57Charge relay systemBy similarity1
Active sitei101Charge relay systemBy similarity1
Active sitei195Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processDigestion

Protein family/group databases

MEROPSiS01.437

Names & Taxonomyi

Protein namesi
Recommended name:
Chymotrypsin B (EC:3.4.21.1)
Cleaved into the following 2 chains:
OrganismiGadus morhua (Atlantic cod)
Taxonomic identifieri8049 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataZeiogadariaGadariaeGadiformesGadoideiGadidaeGadus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000276471 – 13Chymotrypsin B chain AAdd BLAST13
PropeptideiPRO_000002764814 – 151 Publication2
ChainiPRO_000002764916 – 245Chymotrypsin B chain BAdd BLAST230

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi1 ↔ 121PROSITE-ProRule annotation
Disulfide bondi42 ↔ 58PROSITE-ProRule annotation
Disulfide bondi135 ↔ 201PROSITE-ProRule annotation
Disulfide bondi167 ↔ 182PROSITE-ProRule annotation
Disulfide bondi191 ↔ 220PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Structurei

3D structure databases

ProteinModelPortaliP80646
SMRiP80646
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 243Peptidase S1PROSITE-ProRule annotationAdd BLAST228

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
HOVERGENiHBG013304
TreeFamiTF330455

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P80646-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
CGSPAIQPQV TGYARIVNGE EAVPHSWPWQ VSLQQSNGFH FCGGSLINEN
60 70 80 90 100
WVVTAAHCNV RTYHRVIVGE HDKASDENIQ ILKPSMVFTH PKWDSRTINN
110 120 130 140 150
DISLIKLASP AVLGTNVSPV CLGESSDVFA PGMKCVTSGW GLTRYNAPGT
160 170 180 190 200
PNKLQQAALP LMSNEECSQT WGNNMISDVM ICAGAAGATS CMGDSGGPLV
210 220 230 240
CQKDNVWTLV GIVSWGSSRC SVTTPAVYAR VTELRGWVDQ ILAAN
Length:245
Mass (Da):26,260
Last modified:October 1, 1996 - v1
Checksum:i74FE0D425517AB02
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti9 – 11QVT → VIS AA sequence (PubMed:1764912).Curated3
Sequence conflicti26S → T AA sequence (PubMed:1764912).Curated1
Sequence conflicti28 – 29PW → Y AA sequence (PubMed:1764912).Curated2

Similar proteinsi

Cross-referencesi

3D structure databases

ProteinModelPortaliP80646
SMRiP80646
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.437

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
HOVERGENiHBG013304
TreeFamiTF330455

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCTRB_GADMO
AccessioniPrimary (citable) accession number: P80646
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 5, 2017
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
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Main funding by: National Institutes of Health

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