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Protein

Alcohol dehydrogenase class-3

Gene
N/A
Organism
Pisum sativum (Garden pea)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione.1 Publication

Catalytic activityi

A primary alcohol + NAD+ = an aldehyde + NADH.1 Publication
A secondary alcohol + NAD+ = a ketone + NADH.1 Publication
S-(hydroxymethyl)glutathione + NAD(P)+ = S-formylglutathione + NAD(P)H.1 Publication

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Kineticsi

kcat is 190 min(-1) with octanol as substrate (at pH 10). kcat is 110 min(-1) with 12-OH-dodecanoic acid as substrate (at pH 10). kcat is 380 min(-1) with S-hydroxymethylglutathione as substrate (at pH 8).1 Publication
  1. KM=840 µM for octanol (at pH 10)1 Publication
  2. KM=180 µM for 12-OH-dodecanoic acid (at pH 10)1 Publication
  3. KM=6.5 µM for NAD+ (at pH 10)1 Publication
  4. KM=2 µM for S-(hydroxymethyl)glutathione (at pH 8)1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi46Zinc 1; catalyticBy similarity1
    Binding sitei47NADBy similarity1
    Binding sitei48SubstrateBy similarity1
    Metal bindingi68Zinc 1; catalyticBy similarity1
    Binding sitei68SubstrateBy similarity1
    Metal bindingi69Zinc 1By similarity1
    Metal bindingi98Zinc 2By similarity1
    Metal bindingi101Zinc 2By similarity1
    Metal bindingi104Zinc 2By similarity1
    Metal bindingi112Zinc 2By similarity1
    Metal bindingi176Zinc 1; catalyticBy similarity1
    Binding sitei225NADBy similarity1
    Binding sitei230NADBy similarity1
    Binding sitei371NADBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi201 – 206NADBy similarity6
    Nucleotide bindingi294 – 296NADBy similarity3
    Nucleotide bindingi319 – 321NADBy similarity3

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionOxidoreductase
    LigandMetal-binding, NAD, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alcohol dehydrogenase class-3 (EC:1.1.1.11 Publication)
    Alternative name(s):
    Alcohol dehydrogenase class-III
    Glutathione-dependent formaldehyde dehydrogenase (EC:1.1.1.-)
    Short name:
    FALDH
    Short name:
    FDH
    Short name:
    GSH-FDH
    S-(hydroxymethyl)glutathione dehydrogenase (EC:1.1.1.2841 Publication)
    OrganismiPisum sativum (Garden pea)
    Taxonomic identifieri3888 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideae50 kb inversion cladeNPAAA cladeHologaleginaIRL cladeFabeaePisum

    Subcellular locationi

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001607741 – 378Alcohol dehydrogenase class-3Add BLAST378

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei1N-acetylalanine2 Publications1

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PRIDEiP80572

    PTM databases

    iPTMnetiP80572

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP80572
    SMRiP80572
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    CDDicd08300 alcohol_DH_class_III, 1 hit
    InterProiView protein in InterPro
    IPR014183 ADH_3
    IPR013149 ADH_C
    IPR013154 ADH_N
    IPR002328 ADH_Zn_CS
    IPR011032 GroES-like_sf
    IPR036291 NAD(P)-bd_dom_sf
    PfamiView protein in Pfam
    PF08240 ADH_N, 1 hit
    PF00107 ADH_zinc_N, 1 hit
    SUPFAMiSSF50129 SSF50129, 2 hits
    SSF51735 SSF51735, 1 hit
    TIGRFAMsiTIGR02818 adh_III_F_hyde, 1 hit
    PROSITEiView protein in PROSITE
    PS00059 ADH_ZINC, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P80572-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    ATQGQVITCK AAVAWEPNKP LTIEDVEVAP PQANEVRIQI LFTALCHTDA
    60 70 80 90 100
    YTLGGKDPEG LFPCILGHEA AGIVESVGEG VTDVKPGDHV IPSYQAECGE
    110 120 130 140 150
    CKFCKSPKTN LCGKVRAATG VGVMMADRKS RFSVKGKPIY HFMGTSTFSQ
    160 170 180 190 200
    YTVVHDVSVA KIHPDAPLDK VCLLGCGVPT GLGAVWNTAK VEPGSIVAIF
    210 220 230 240 250
    GLGTVGLAVA EGAKSAGASR IIGIDIDSNK YDTAKNFGVT EFINPKDHEK
    260 270 280 290 300
    PIQQVIIDLT DGGVDYSFEC LGNVSVMRSA LECCHKGWGT SVIVGVAASG
    310 320 330 340 350
    QEISTRPFQL VTGRVWKGTA FGGFKSRSQV PWLVEKYLKK EIKVDEYITH
    360 370
    NLTLLEINKA FDLLHEGQCL RCVLAVHD
    Length:378
    Mass (Da):40,489
    Last modified:July 15, 1998 - v1
    Checksum:i9ED1C511219FF45B
    GO

    Sequence databases

    PIRiS66198

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    PIRiS66198

    3D structure databases

    ProteinModelPortaliP80572
    SMRiP80572
    ModBaseiSearch...
    MobiDBiSearch...

    PTM databases

    iPTMnetiP80572

    Proteomic databases

    PRIDEiP80572

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Family and domain databases

    CDDicd08300 alcohol_DH_class_III, 1 hit
    InterProiView protein in InterPro
    IPR014183 ADH_3
    IPR013149 ADH_C
    IPR013154 ADH_N
    IPR002328 ADH_Zn_CS
    IPR011032 GroES-like_sf
    IPR036291 NAD(P)-bd_dom_sf
    PfamiView protein in Pfam
    PF08240 ADH_N, 1 hit
    PF00107 ADH_zinc_N, 1 hit
    SUPFAMiSSF50129 SSF50129, 2 hits
    SSF51735 SSF51735, 1 hit
    TIGRFAMsiTIGR02818 adh_III_F_hyde, 1 hit
    PROSITEiView protein in PROSITE
    PS00059 ADH_ZINC, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiADHX_PEA
    AccessioniPrimary (citable) accession number: P80572
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 15, 1998
    Last modified: October 10, 2018
    This is version 98 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

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