UniProtKB - P80316 (TCPE_MOUSE)
Protein
T-complex protein 1 subunit epsilon
Gene
Cct5
Organism
Mus musculus (Mouse)
Status
Functioni
Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. The TRiC complex plays a role in the folding of actin and tubulin.By similarity
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- beta-tubulin binding Source: MGI
- G-protein beta-subunit binding Source: UniProtKB
- mRNA 3'-UTR binding Source: MGI
- mRNA 5'-UTR binding Source: MGI
- unfolded protein binding Source: GO_Central
GO - Biological processi
- binding of sperm to zona pellucida Source: MGI
- positive regulation of establishment of protein localization to telomere Source: MGI
- positive regulation of telomere maintenance via telomerase Source: MGI
- protein folding Source: GO_Central
- protein stabilization Source: MGI
- response to virus Source: Ensembl
- toxin transport Source: MGI
Keywordsi
Molecular function | Chaperone |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-390471, Association of TriC/CCT with target proteins during biosynthesis R-MMU-5620922, BBSome-mediated cargo-targeting to cilium R-MMU-6814122, Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding |
Names & Taxonomyi
Protein namesi | Recommended name: T-complex protein 1 subunit epsilonShort name: TCP-1-epsilon Alternative name(s): CCT-epsilon |
Gene namesi | Name:Cct5 Synonyms:Ccte, Kiaa0098 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:107185, Cct5 |
Subcellular locationi
Cytoskeleton
- centrosome By similarity
Other locations
- Cytoplasm By similarity
Cytoskeleton
- centrosome Source: MGI
- microtubule Source: MGI
Cytosol
- chaperonin-containing T-complex Source: MGI
Other locations
- cell body Source: MGI
- myelin sheath Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, CytoskeletonPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000128347 | 2 – 541 | T-complex protein 1 subunit epsilonAdd BLAST | 540 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Cross-linki | 20 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 26 | PhosphoserineBy similarity | 1 | |
Cross-linki | 210 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 214 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 265 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 275 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 279 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 346 | PhosphoserineBy similarity | 1 | |
Cross-linki | 392 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 539 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
The N-terminus is blocked.
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | P80316 |
jPOSTi | P80316 |
MaxQBi | P80316 |
PaxDbi | P80316 |
PeptideAtlasi | P80316 |
PRIDEi | P80316 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00116279 P80316 |
SWISS-2DPAGEi | P80316 |
PTM databases
iPTMneti | P80316 |
PhosphoSitePlusi | P80316 |
SwissPalmi | P80316 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000022234, Expressed in mandibular prominence and 309 other tissues |
ExpressionAtlasi | P80316, baseline and differential |
Genevisiblei | P80316, MM |
Interactioni
Subunit structurei
Component of the chaperonin-containing T-complex (TRiC), a heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.
Interacts with PACRG (By similarity).
Interacts with DNAAF4 (PubMed:23872636).
By similarity1 PublicationBinary interactionsi
P80316
With | #Exp. | IntAct |
---|---|---|
Theg [Q9JMB1] | 2 | EBI-772379,EBI-1390549 |
GO - Molecular functioni
- beta-tubulin binding Source: MGI
- G-protein beta-subunit binding Source: UniProtKB
- unfolded protein binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 198568, 37 interactors |
CORUMi | P80316 |
IntActi | P80316, 13 interactors |
MINTi | P80316 |
STRINGi | 10090.ENSMUSP00000022842 |
Miscellaneous databases
RNActi | P80316, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the TCP-1 chaperonin family.Curated
Phylogenomic databases
eggNOGi | KOG0357, Eukaryota |
GeneTreei | ENSGT00550000074988 |
HOGENOMi | CLU_008891_7_2_1 |
InParanoidi | P80316 |
OMAi | AHDQFAN |
OrthoDBi | 511484at2759 |
PhylomeDBi | P80316 |
TreeFami | TF105638 |
Family and domain databases
Gene3Di | 1.10.560.10, 1 hit 3.30.260.10, 1 hit 3.50.7.10, 1 hit |
InterProi | View protein in InterPro IPR017998, Chaperone_TCP-1 IPR002194, Chaperonin_TCP-1_CS IPR002423, Cpn60/TCP-1 IPR027409, GroEL-like_apical_dom_sf IPR027413, GROEL-like_equatorial_sf IPR027410, TCP-1-like_intermed_sf |
Pfami | View protein in Pfam PF00118, Cpn60_TCP1, 1 hit |
PRINTSi | PR00304, TCOMPLEXTCP1 |
SUPFAMi | SSF48592, SSF48592, 1 hit SSF52029, SSF52029, 1 hit SSF54849, SSF54849, 1 hit |
PROSITEi | View protein in PROSITE PS00750, TCP1_1, 1 hit PS00751, TCP1_2, 1 hit PS00995, TCP1_3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P80316-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASVGTLAFD EYGRPFLIIK DQDRKSRLMG LEALKSHIMA AKAVANTMRT
60 70 80 90 100
SLGPNGLDKM MVDKDGDVTI TNDGATILSM MDVDHQIAKL MVELSKSQDD
110 120 130 140 150
EIGDGTTGVV VLAGALLEEA EQLLDRGIHP IRIADGYEQA ARIAIQHLDK
160 170 180 190 200
ISDKVLVDIN NPEPLIQTAK TTLGSKVINS CHRQMAEIAV NAVLTVADME
210 220 230 240 250
RRDVDFELIK VEGKVGGRLE DTKLIKGVIV DKDFSHPQMP KKVVDAKIAI
260 270 280 290 300
LTCPFEPPKP KTKHKLDVMS VEDYKALQKY EKEKFEEMIK QIKETGANLA
310 320 330 340 350
ICQWGFDDEA NHLLLQNGLP AVRWVGGPEI ELIAIATGGR IVPRFSELTS
360 370 380 390 400
EKLGFAGVVQ EISFGTTKDK MLVIEKCKNS RAVTIFIRGG NKMIIEEAKR
410 420 430 440 450
SLHDALCVIR NLIRDNRVVY GGGAAEISCA LAVSQEADKC PTLEQYAMRA
460 470 480 490 500
FADALEVIPM ALSENSGMNP IQTMTEVRAR QVKESNPALG IDCLHKGSND
510 520 530 540
MQYQHVIETL IGKKQQISLA TQMVRMILKI DDIRKPGESE E
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE0CZA1 | E0CZA1_MOUSE | T-complex protein 1 subunit epsilon | Cct5 | 199 | Annotation score: |
Sequence cautioni
The sequence BAC97866 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 27 | R → H in BAE23007 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 48 | M → I in BAE27146 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 74 | G → D in BAE32250 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 125 | D → E in BAE27146 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 191 | N → D in BAE39906 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 493 | C → Y in BAE29518 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z31555 mRNA Translation: CAA83430.1 AB022158 Genomic DNA Translation: BAA81876.1 AK129056 mRNA Translation: BAC97866.1 Different initiation. AK088184 mRNA Translation: BAC40194.1 AK133857 mRNA Translation: BAE21891.1 AK136493 mRNA Translation: BAE23007.1 AK145445 mRNA Translation: BAE26441.1 AK146405 mRNA Translation: BAE27146.1 AK146425 mRNA Translation: BAE27159.1 AK150390 mRNA Translation: BAE29518.1 AK153911 mRNA Translation: BAE32250.1 AK167895 mRNA Translation: BAE39906.1 BC094427 mRNA Translation: AAH94427.1 |
CCDSi | CCDS27411.1 |
PIRi | S43061 |
RefSeqi | NP_001334964.1, NM_001348035.1 NP_031663.1, NM_007637.3 |
Genome annotation databases
Ensembli | ENSMUST00000022842; ENSMUSP00000022842; ENSMUSG00000022234 |
GeneIDi | 12465 |
KEGGi | mmu:12465 |
UCSCi | uc007vkj.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z31555 mRNA Translation: CAA83430.1 AB022158 Genomic DNA Translation: BAA81876.1 AK129056 mRNA Translation: BAC97866.1 Different initiation. AK088184 mRNA Translation: BAC40194.1 AK133857 mRNA Translation: BAE21891.1 AK136493 mRNA Translation: BAE23007.1 AK145445 mRNA Translation: BAE26441.1 AK146405 mRNA Translation: BAE27146.1 AK146425 mRNA Translation: BAE27159.1 AK150390 mRNA Translation: BAE29518.1 AK153911 mRNA Translation: BAE32250.1 AK167895 mRNA Translation: BAE39906.1 BC094427 mRNA Translation: AAH94427.1 |
CCDSi | CCDS27411.1 |
PIRi | S43061 |
RefSeqi | NP_001334964.1, NM_001348035.1 NP_031663.1, NM_007637.3 |
3D structure databases
SMRi | P80316 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 198568, 37 interactors |
CORUMi | P80316 |
IntActi | P80316, 13 interactors |
MINTi | P80316 |
STRINGi | 10090.ENSMUSP00000022842 |
PTM databases
iPTMneti | P80316 |
PhosphoSitePlusi | P80316 |
SwissPalmi | P80316 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00116279 P80316 |
SWISS-2DPAGEi | P80316 |
Proteomic databases
EPDi | P80316 |
jPOSTi | P80316 |
MaxQBi | P80316 |
PaxDbi | P80316 |
PeptideAtlasi | P80316 |
PRIDEi | P80316 |
Protocols and materials databases
Antibodypediai | 1210, 317 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000022842; ENSMUSP00000022842; ENSMUSG00000022234 |
GeneIDi | 12465 |
KEGGi | mmu:12465 |
UCSCi | uc007vkj.1, mouse |
Organism-specific databases
CTDi | 22948 |
MGIi | MGI:107185, Cct5 |
Rougei | Search... |
Phylogenomic databases
eggNOGi | KOG0357, Eukaryota |
GeneTreei | ENSGT00550000074988 |
HOGENOMi | CLU_008891_7_2_1 |
InParanoidi | P80316 |
OMAi | AHDQFAN |
OrthoDBi | 511484at2759 |
PhylomeDBi | P80316 |
TreeFami | TF105638 |
Enzyme and pathway databases
Reactomei | R-MMU-390471, Association of TriC/CCT with target proteins during biosynthesis R-MMU-5620922, BBSome-mediated cargo-targeting to cilium R-MMU-6814122, Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding |
Miscellaneous databases
BioGRID-ORCSi | 12465, 8 hits in 16 CRISPR screens |
ChiTaRSi | Cct5, mouse |
PROi | PR:P80316 |
RNActi | P80316, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000022234, Expressed in mandibular prominence and 309 other tissues |
ExpressionAtlasi | P80316, baseline and differential |
Genevisiblei | P80316, MM |
Family and domain databases
Gene3Di | 1.10.560.10, 1 hit 3.30.260.10, 1 hit 3.50.7.10, 1 hit |
InterProi | View protein in InterPro IPR017998, Chaperone_TCP-1 IPR002194, Chaperonin_TCP-1_CS IPR002423, Cpn60/TCP-1 IPR027409, GroEL-like_apical_dom_sf IPR027413, GROEL-like_equatorial_sf IPR027410, TCP-1-like_intermed_sf |
Pfami | View protein in Pfam PF00118, Cpn60_TCP1, 1 hit |
PRINTSi | PR00304, TCOMPLEXTCP1 |
SUPFAMi | SSF48592, SSF48592, 1 hit SSF52029, SSF52029, 1 hit SSF54849, SSF54849, 1 hit |
PROSITEi | View protein in PROSITE PS00750, TCP1_1, 1 hit PS00751, TCP1_2, 1 hit PS00995, TCP1_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TCPE_MOUSE | |
Accessioni | P80316Primary (citable) accession number: P80316 Secondary accession number(s): Q3TIE0 Q6ZQJ1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | June 1, 1994 | |
Last modified: | December 2, 2020 | |
This is version 175 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families