UniProtKB - P80315 (TCPD_MOUSE)
Protein
T-complex protein 1 subunit delta
Gene
Cct4
Organism
Mus musculus (Mouse)
Status
Functioni
Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. The TRiC complex plays a role in the folding of actin and tubulin.By similarity
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- unfolded protein binding Source: GO_Central
GO - Biological processi
- binding of sperm to zona pellucida Source: MGI
- positive regulation of establishment of protein localization to telomere Source: MGI
- positive regulation of telomerase activity Source: MGI
- positive regulation of telomerase RNA localization to Cajal body Source: MGI
- positive regulation of telomere maintenance via telomerase Source: MGI
- protein folding Source: GO_Central
- protein stabilization Source: MGI
- scaRNA localization to Cajal body Source: MGI
- toxin transport Source: MGI
Keywordsi
Molecular function | Chaperone |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-390471, Association of TriC/CCT with target proteins during biosynthesis R-MMU-5620922, BBSome-mediated cargo-targeting to cilium R-MMU-6814122, Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding |
Names & Taxonomyi
Protein namesi | Recommended name: T-complex protein 1 subunit deltaShort name: TCP-1-delta Alternative name(s): A45 CCT-delta |
Gene namesi | Name:Cct4 Synonyms:Cctd |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:104689, Cct4 |
Subcellular locationi
Cytoskeleton
- centrosome 1 Publication
- cilium basal body 1 Publication
Other locations
- Cytoplasm 1 Publication
- Melanosome By similarity
Cytoskeleton
- centrosome Source: MGI
- microtubule Source: MGI
Cytosol
- chaperonin-containing T-complex Source: MGI
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
Other locations
- cell body Source: MGI
- cilium Source: UniProtKB-KW
- melanosome Source: UniProtKB-SubCell
- zona pellucida receptor complex Source: MGI
Keywords - Cellular componenti
Cell projection, Cilium, Cytoplasm, CytoskeletonPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000128333 | 1 – 539 | T-complex protein 1 subunit deltaAdd BLAST | 539 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 19 | Omega-N-methylarginineBy similarity | 1 | |
Modified residuei | 21 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 36 | PhosphoserineBy similarity | 1 | |
Modified residuei | 184 | PhosphoserineBy similarity | 1 | |
Modified residuei | 202 | PhosphoserineBy similarity | 1 | |
Modified residuei | 288 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 302 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 319 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 326 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 444 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
The N-terminus is blocked.
Keywords - PTMi
Acetylation, Methylation, PhosphoproteinProteomic databases
CPTACi | non-CPTAC-3951 |
EPDi | P80315 |
jPOSTi | P80315 |
PaxDbi | P80315 |
PeptideAtlasi | P80315 |
PRIDEi | P80315 |
2D gel databases
REPRODUCTION-2DPAGEi | P80315 |
PTM databases
iPTMneti | P80315 |
PhosphoSitePlusi | P80315 |
SwissPalmi | P80315 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000007739, Expressed in indifferent gonad and 303 other tissues |
ExpressionAtlasi | P80315, baseline and differential |
Genevisiblei | P80315, MM |
Interactioni
Subunit structurei
Component of the chaperonin-containing T-complex (TRiC), a heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter.
Interacts with PACRG (By similarity).
Interacts with DNAAF4 (PubMed:23872636).
By similarity1 PublicationGO - Molecular functioni
- unfolded protein binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 198567, 79 interactors |
CORUMi | P80315 |
IntActi | P80315, 56 interactors |
MINTi | P80315 |
STRINGi | 10090.ENSMUSP00000133523 |
Miscellaneous databases
RNActi | P80315, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the TCP-1 chaperonin family.Curated
Phylogenomic databases
eggNOGi | KOG0358, Eukaryota |
GeneTreei | ENSGT00550000074956 |
HOGENOMi | CLU_008891_9_1_1 |
InParanoidi | P80315 |
OMAi | TGIQNMG |
OrthoDBi | 511484at2759 |
PhylomeDBi | P80315 |
TreeFami | TF106332 |
Family and domain databases
CDDi | cd03338, TCP1_delta, 1 hit |
Gene3Di | 1.10.560.10, 2 hits 3.50.7.10, 1 hit |
InterProi | View protein in InterPro IPR012717, Chap_CCT_delta IPR017998, Chaperone_TCP-1 IPR002194, Chaperonin_TCP-1_CS IPR002423, Cpn60/TCP-1 IPR027409, GroEL-like_apical_dom_sf IPR027413, GROEL-like_equatorial_sf IPR027410, TCP-1-like_intermed_sf |
Pfami | View protein in Pfam PF00118, Cpn60_TCP1, 1 hit |
PRINTSi | PR00304, TCOMPLEXTCP1 |
SUPFAMi | SSF48592, SSF48592, 1 hit SSF52029, SSF52029, 1 hit SSF54849, SSF54849, 1 hit |
TIGRFAMsi | TIGR02342, chap_CCT_delta, 1 hit |
PROSITEi | View protein in PROSITE PS00750, TCP1_1, 1 hit PS00751, TCP1_2, 1 hit PS00995, TCP1_3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
P80315-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MPENVASRSG APTAGPGSRG KSAYQDRDKP AQIRFSNISA AKAVADAIRT
60 70 80 90 100
SLGPKGMDKM IQDGKGDVTI TNDGATILKQ MQVLHPAARM LVELSKAQDI
110 120 130 140 150
EAGDGTTSVV IIAGSLLDSC TKLLQKGIHP TIISESFQKA LEKGLEILTD
160 170 180 190 200
MSRPVQLSDR ETLLNSATTS LNSKVVSQYS SLLSPMSVNA VMKVIDPATA
210 220 230 240 250
TSVDLRDIKI VKKLGGTIDD CELVEGLVLT QKVANSGITR VEKAKIGLIQ
260 270 280 290 300
FCLSAPKTDM DNQIVVSDYA QMDRVLREER AYILNLVKQI KKTGCNVLLI
310 320 330 340 350
QKSILRDALS DLALHFLNKM KIMVVKDVER EDIEFICKTI GTKPVAHIDQ
360 370 380 390 400
FTADMLGSAE LAEEVSLNGS GKLFKITGCT SPGKTVTIVV RGSNKLVIEE
410 420 430 440 450
AERSIHDALC VIRCLVKKRA LIAGGGAPEI ELALRLTEYS RTLSGMESYC
460 470 480 490 500
VRAFADAMEV IPSTLAENAG LNPISTVTEL RNRHAQGEKT TGINVRKGGI
510 520 530
SNILEEMVVQ PLLVSVSALT LATETVRSIL KIDDVVNTR
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG5E839 | G5E839_MOUSE | T-complex protein 1 subunit delta | Cct4 | 509 | Annotation score: | ||
G3UYW5 | G3UYW5_MOUSE | T-complex protein 1 subunit delta | Cct4 | 120 | Annotation score: | ||
G3UXF3 | G3UXF3_MOUSE | T-complex protein 1 subunit delta | Cct4 | 47 | Annotation score: | ||
G3UXG2 | G3UXG2_MOUSE | T-complex protein 1 subunit delta | Cct4 | 59 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 405 | I → V in AAA37418 (PubMed:7919332).Curated | 1 | |
Sequence conflicti | 443 | L → P in AAA37418 (PubMed:7919332).Curated | 1 | |
Sequence conflicti | 453 | A → R in AAA37418 (PubMed:7919332).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L25913 mRNA Translation: AAA37418.1 Z31554 mRNA Translation: CAA83429.1 AB022157 Genomic DNA Translation: BAA81875.1 BC054773 mRNA Translation: AAH54773.1 |
CCDSi | CCDS24474.1 |
PIRi | S43060 |
RefSeqi | NP_033967.1, NM_009837.1 |
Genome annotation databases
Ensembli | ENSMUST00000173867; ENSMUSP00000133523; ENSMUSG00000007739 |
GeneIDi | 12464 |
KEGGi | mmu:12464 |
UCSCi | uc007ien.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L25913 mRNA Translation: AAA37418.1 Z31554 mRNA Translation: CAA83429.1 AB022157 Genomic DNA Translation: BAA81875.1 BC054773 mRNA Translation: AAH54773.1 |
CCDSi | CCDS24474.1 |
PIRi | S43060 |
RefSeqi | NP_033967.1, NM_009837.1 |
3D structure databases
SMRi | P80315 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 198567, 79 interactors |
CORUMi | P80315 |
IntActi | P80315, 56 interactors |
MINTi | P80315 |
STRINGi | 10090.ENSMUSP00000133523 |
PTM databases
iPTMneti | P80315 |
PhosphoSitePlusi | P80315 |
SwissPalmi | P80315 |
2D gel databases
REPRODUCTION-2DPAGEi | P80315 |
Proteomic databases
CPTACi | non-CPTAC-3951 |
EPDi | P80315 |
jPOSTi | P80315 |
PaxDbi | P80315 |
PeptideAtlasi | P80315 |
PRIDEi | P80315 |
Protocols and materials databases
Antibodypediai | 15920, 241 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000173867; ENSMUSP00000133523; ENSMUSG00000007739 |
GeneIDi | 12464 |
KEGGi | mmu:12464 |
UCSCi | uc007ien.1, mouse |
Organism-specific databases
CTDi | 10575 |
MGIi | MGI:104689, Cct4 |
Phylogenomic databases
eggNOGi | KOG0358, Eukaryota |
GeneTreei | ENSGT00550000074956 |
HOGENOMi | CLU_008891_9_1_1 |
InParanoidi | P80315 |
OMAi | TGIQNMG |
OrthoDBi | 511484at2759 |
PhylomeDBi | P80315 |
TreeFami | TF106332 |
Enzyme and pathway databases
Reactomei | R-MMU-390471, Association of TriC/CCT with target proteins during biosynthesis R-MMU-5620922, BBSome-mediated cargo-targeting to cilium R-MMU-6814122, Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding |
Miscellaneous databases
BioGRID-ORCSi | 12464, 11 hits in 18 CRISPR screens |
ChiTaRSi | Cct4, mouse |
PROi | PR:P80315 |
RNActi | P80315, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000007739, Expressed in indifferent gonad and 303 other tissues |
ExpressionAtlasi | P80315, baseline and differential |
Genevisiblei | P80315, MM |
Family and domain databases
CDDi | cd03338, TCP1_delta, 1 hit |
Gene3Di | 1.10.560.10, 2 hits 3.50.7.10, 1 hit |
InterProi | View protein in InterPro IPR012717, Chap_CCT_delta IPR017998, Chaperone_TCP-1 IPR002194, Chaperonin_TCP-1_CS IPR002423, Cpn60/TCP-1 IPR027409, GroEL-like_apical_dom_sf IPR027413, GROEL-like_equatorial_sf IPR027410, TCP-1-like_intermed_sf |
Pfami | View protein in Pfam PF00118, Cpn60_TCP1, 1 hit |
PRINTSi | PR00304, TCOMPLEXTCP1 |
SUPFAMi | SSF48592, SSF48592, 1 hit SSF52029, SSF52029, 1 hit SSF54849, SSF54849, 1 hit |
TIGRFAMsi | TIGR02342, chap_CCT_delta, 1 hit |
PROSITEi | View protein in PROSITE PS00750, TCP1_1, 1 hit PS00751, TCP1_2, 1 hit PS00995, TCP1_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TCPD_MOUSE | |
Accessioni | P80315Primary (citable) accession number: P80315 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 1994 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 177 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families