UniProtKB - P80210 (PURA_YEAST)
Protein
Adenylosuccinate synthetase
Gene
ADE12
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.2 Publications
Miscellaneous
Present with 56800 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- EC:6.3.4.4UniRule annotation
Cofactori
Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation
Activity regulationi
Inhibited by GMP. Inhibited by chloride. Inhibited in a highly specific manner by the binding of a 44-base DNA oligonucleotide carrying the ARS core consensus sequence.1 Publication
Kineticsi
- KM=1650 µM for L-aspartate2 Publications
- KM=200 µM for IMP2 Publications
- KM=1650 µM for GTP2 Publications
pH dependencei
Optimum pH is 8.0.2 Publications
Temperature dependencei
Optimum temperature is 35 degrees Celsius.2 Publications
: AMP biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes AMP from IMP.UniRule annotationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- Adenylosuccinate synthetase (ADE12)
- Adenylosuccinate lyase (ADE13)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from IMP, the pathway AMP biosynthesis via de novo pathway and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 12 | Proton acceptorUniRule annotation | 1 | |
Metal bindingi | 12 | MagnesiumUniRule annotation | 1 | |
Metal bindingi | 39 | Magnesium; via carbonyl oxygenUniRule annotation | 1 | |
Active sitei | 40 | Proton donorUniRule annotation | 1 | |
Binding sitei | 134 | IMPUniRule annotation | 1 | |
Binding sitei | 148 | IMP; shared with dimeric partnerUniRule annotation | 1 | |
Binding sitei | 230 | IMPUniRule annotation | 1 | |
Binding sitei | 245 | IMPUniRule annotation | 1 | |
Binding sitei | 309 | IMPUniRule annotation | 1 | |
Binding sitei | 311 | GTPUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 11 – 17 | GTPUniRule annotation | 7 | |
Nucleotide bindingi | 39 – 41 | GTPUniRule annotation | 3 | |
Nucleotide bindingi | 337 – 339 | GTPUniRule annotation | 3 | |
Nucleotide bindingi | 419 – 421 | GTPUniRule annotation | 3 |
GO - Molecular functioni
- adenylosuccinate synthase activity Source: SGD
- DNA replication origin binding Source: SGD
- GTP binding Source: UniProtKB-UniRule
- magnesium ion binding Source: UniProtKB-UniRule
GO - Biological processi
- 'de novo' AMP biosynthetic process Source: GO_Central
- IMP metabolic process Source: GO_Central
- purine nucleotide biosynthetic process Source: SGD
Keywordsi
Molecular function | Ligase |
Biological process | Purine biosynthesis |
Ligand | GTP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:YNL220W-MONOMER |
Reactomei | R-SCE-73817, Purine ribonucleoside monophosphate biosynthesis |
UniPathwayi | UPA00075;UER00335 |
Names & Taxonomyi
Protein namesi | Recommended name: Adenylosuccinate synthetaseUniRule annotation (EC:6.3.4.4UniRule annotation)Short name: AMPSaseUniRule annotation Short name: AS-synthetase Short name: AdSSUniRule annotation Alternative name(s): IMP--aspartate ligaseUniRule annotation |
Gene namesi | Name:ADE12UniRule annotation Ordered Locus Names:YNL220W ORF Names:N1290 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YNL220W |
SGDi | S000005164, ADE12 |
Subcellular locationi
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed2 Publications | |||
ChainiPRO_0000095138 | 2 – 433 | Adenylosuccinate synthetaseAdd BLAST | 432 |
Proteomic databases
MaxQBi | P80210 |
PaxDbi | P80210 |
PRIDEi | P80210 |
PTM databases
iPTMneti | P80210 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotation3 PublicationsBinary interactionsi
Hide detailsProtein-protein interaction databases
BioGRIDi | 35616, 155 interactors |
DIPi | DIP-4286N |
IntActi | P80210, 8 interactors |
MINTi | P80210 |
STRINGi | 4932.YNL220W |
Miscellaneous databases
RNActi | P80210, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 12 – 15 | IMP bindingUniRule annotation | 4 | |
Regioni | 37 – 40 | IMP bindingUniRule annotation | 4 | |
Regioni | 305 – 311 | Substrate bindingUniRule annotation | 7 |
Sequence similaritiesi
Belongs to the adenylosuccinate synthetase family.UniRule annotation
Phylogenomic databases
eggNOGi | KOG1355, Eukaryota |
GeneTreei | ENSGT00390000015553 |
HOGENOMi | CLU_029848_3_2_1 |
InParanoidi | P80210 |
OMAi | FHHAKPI |
Family and domain databases
CDDi | cd03108, AdSS, 1 hit |
Gene3Di | 1.10.300.10, 1 hit 3.40.440.10, 1 hit 3.90.170.10, 1 hit |
HAMAPi | MF_00011, Adenylosucc_synth, 1 hit |
InterProi | View protein in InterPro IPR018220, Adenylosuccin_syn_GTP-bd IPR033128, Adenylosuccin_syn_Lys_AS IPR042109, Adenylosuccinate_synth_dom1 IPR042110, Adenylosuccinate_synth_dom2 IPR042111, Adenylosuccinate_synth_dom3 IPR001114, Adenylosuccinate_synthetase IPR027417, P-loop_NTPase |
PANTHERi | PTHR11846, PTHR11846, 1 hit |
Pfami | View protein in Pfam PF00709, Adenylsucc_synt, 1 hit |
SMARTi | View protein in SMART SM00788, Adenylsucc_synt, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00184, purA, 1 hit |
PROSITEi | View protein in PROSITE PS01266, ADENYLOSUCCIN_SYN_1, 1 hit PS00513, ADENYLOSUCCIN_SYN_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P80210-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVNVVLGSQW GDEGKGKLVD LLVGKYDIVA RCAGGNNAGH TIVVDGVKYD
60 70 80 90 100
FHMLPSGLVN PNCQNLLGNG VVIHVPSFFK ELETLEAKGL KNARSRLFVS
110 120 130 140 150
SRAHLVFDFH QVTDKLRELE LSGRSKDGKN IGTTGKGIGP TYSTKASRSG
160 170 180 190 200
LRVHHLVNDQ PGAWEEFVAR YKRLLETRRQ RYGDFEYDFE AKLAEYKKLR
210 220 230 240 250
EQLKPFVVDS VVFMHNAIEA KKKILVEGAN ALMLDIDFGT YPYVTSSNTG
260 270 280 290 300
IGGVLTGLGI PPRTIDEIYG VVKAYTTRVG EGPFPTEQLN ENGEKLQTIG
310 320 330 340 350
AEFGVTTGRK RRCGWLDLVV LKYSTLINGY TSLNITKLDV LDTFKEIPVG
360 370 380 390 400
ISYSIQGKKL DLFPEDLNIL GKVEVEYKVL PGWDQDITKI TKYEDLPENA
410 420 430
KKYLKYIEDF VGVPVEWVGT GPARESMLHK EIK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 237 | D → G AA sequence (PubMed:8376380).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L22185 Genomic DNA Translation: AAA91338.1 Z48671 Genomic DNA Translation: CAA88590.1 Z71496 Genomic DNA Translation: CAA96123.1 BK006947 Genomic DNA Translation: DAA10336.1 |
PIRi | S48515 |
RefSeqi | NP_014179.1, NM_001183058.1 |
Genome annotation databases
EnsemblFungii | YNL220W_mRNA; YNL220W; YNL220W |
GeneIDi | 855501 |
KEGGi | sce:YNL220W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | L22185 Genomic DNA Translation: AAA91338.1 Z48671 Genomic DNA Translation: CAA88590.1 Z71496 Genomic DNA Translation: CAA96123.1 BK006947 Genomic DNA Translation: DAA10336.1 |
PIRi | S48515 |
RefSeqi | NP_014179.1, NM_001183058.1 |
3D structure databases
SMRi | P80210 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 35616, 155 interactors |
DIPi | DIP-4286N |
IntActi | P80210, 8 interactors |
MINTi | P80210 |
STRINGi | 4932.YNL220W |
PTM databases
iPTMneti | P80210 |
Proteomic databases
MaxQBi | P80210 |
PaxDbi | P80210 |
PRIDEi | P80210 |
Genome annotation databases
EnsemblFungii | YNL220W_mRNA; YNL220W; YNL220W |
GeneIDi | 855501 |
KEGGi | sce:YNL220W |
Organism-specific databases
EuPathDBi | FungiDB:YNL220W |
SGDi | S000005164, ADE12 |
Phylogenomic databases
eggNOGi | KOG1355, Eukaryota |
GeneTreei | ENSGT00390000015553 |
HOGENOMi | CLU_029848_3_2_1 |
InParanoidi | P80210 |
OMAi | FHHAKPI |
Enzyme and pathway databases
UniPathwayi | UPA00075;UER00335 |
BioCyci | MetaCyc:YNL220W-MONOMER |
Reactomei | R-SCE-73817, Purine ribonucleoside monophosphate biosynthesis |
Miscellaneous databases
PROi | PR:P80210 |
RNActi | P80210, protein |
Family and domain databases
CDDi | cd03108, AdSS, 1 hit |
Gene3Di | 1.10.300.10, 1 hit 3.40.440.10, 1 hit 3.90.170.10, 1 hit |
HAMAPi | MF_00011, Adenylosucc_synth, 1 hit |
InterProi | View protein in InterPro IPR018220, Adenylosuccin_syn_GTP-bd IPR033128, Adenylosuccin_syn_Lys_AS IPR042109, Adenylosuccinate_synth_dom1 IPR042110, Adenylosuccinate_synth_dom2 IPR042111, Adenylosuccinate_synth_dom3 IPR001114, Adenylosuccinate_synthetase IPR027417, P-loop_NTPase |
PANTHERi | PTHR11846, PTHR11846, 1 hit |
Pfami | View protein in Pfam PF00709, Adenylsucc_synt, 1 hit |
SMARTi | View protein in SMART SM00788, Adenylsucc_synt, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00184, purA, 1 hit |
PROSITEi | View protein in PROSITE PS01266, ADENYLOSUCCIN_SYN_1, 1 hit PS00513, ADENYLOSUCCIN_SYN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PURA_YEAST | |
Accessioni | P80210Primary (citable) accession number: P80210 Secondary accession number(s): D6W0X0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 1, 1993 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 185 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Yeast chromosome XIV
Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD