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Protein

Actin-fragmin kinase

Gene
N/A
Organism
Physarum polycephalum (Slime mold)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has a role in the regulation of microfilament formation. Phosphorylates the actin-fragmin complex on threonine residues, in vitro.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Activity regulationi

Inhibited by staurosporine.1 Publication

Kineticsi

  1. KM=190 nM for actin-fragmin complex1 Publication
  2. KM=25 µM for ATP1 Publication
  1. Vmax=83 µmol/min/mg enzyme1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei82ATP; via amide nitrogen1
Binding sitei98ATP1
Binding sitei163ATP; via carbonyl oxygen1
Binding sitei165ATP; via amide nitrogen1
Binding sitei232ATP1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi178 – 185ATPSequence analysis8

GO - Molecular functioni

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKiP80197

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-fragmin kinase (EC:2.7.11.1)
Short name:
AFK
OrganismiPhysarum polycephalum (Slime mold)
Taxonomic identifieri5791 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaMyxogastriaMyxogastromycetidaePhysariidaPhysaraceaePhysarum

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000860441 – 737Actin-fragmin kinaseAdd BLAST737

Interactioni

Subunit structurei

Monomer.3 Publications

Structurei

Secondary structure

1737
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP80197
SMRiP80197
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP80197

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati431 – 480Kelch 1Add BLAST50
Repeati482 – 533Kelch 2Add BLAST52
Repeati534 – 584Kelch 3Add BLAST51
Repeati586 – 635Kelch 4Add BLAST50
Repeati646 – 693Kelch 5Add BLAST48

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi350 – 365Poly-SerAdd BLAST16

Sequence similaritiesi

Keywords - Domaini

Kelch repeat, Repeat

Family and domain databases

CDDicd05124 AFK, 1 hit
Gene3Di1.10.1070.11, 1 hit
2.120.10.80, 1 hit
InterProiView protein in InterPro
IPR015275 Actin-fragmin_kin_cat_dom
IPR015915 Kelch-typ_b-propeller
IPR011009 Kinase-like_dom_sf
IPR036940 PI3/4_kinase_cat_sf
PfamiView protein in Pfam
PF09192 Act-Frag_cataly, 1 hit
SUPFAMiSSF117281 SSF117281, 1 hit
SSF56112 SSF56112, 1 hit

Sequencei

Sequence statusi: Complete.

P80197-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAGALWEIEK ELFTKLPAPS SAINSHLQPA KPKVPQKKPS KWDPPAEFKV
60 70 80 90 100
DLSTAVSYND IGDINWKNLQ QFKGIERSEK GTEGLFFVET ESGVFIVKRS
110 120 130 140 150
TNIESETFCS LLCMRLGLHA PKVRVVSSNS EEGTNMLECL AAIDKSFRVI
160 170 180 190 200
TTLANQANIL LMELVRGITL NKLTTTSAPE VLTKSTMQQL GSLMALDVIV
210 220 230 240 250
NNSDRLPIAW TNEGNLDNIM LSERGATVVP IDSKIIPLDA SHPHGERVRE
260 270 280 290 300
LLRTLIAHPG HESSQFHSIR DIITLYTGYD VGTEGSISMQ EGFLATVREC
310 320 330 340 350
ASFDLDAFER ELLSWQESLQ KCHNLSISPQ AIPFILRMLR IFHDAIHNPS
360 370 380 390 400
PSSPSPSPSS SSSTSHPTPA SSSTSSTLPS SIPSSSNTSP PPASSSESLV
410 420 430 440 450
GVEECAWLKV VVPNEKPAPR RYHSGVLYEG KLYVFGGVCI KTASNDFYVF
460 470 480 490 500
DFAKKKWSIV VAQGEAPSPR CGHSATVYGG KMWIFGGHNN NKQPYSDLYT
510 520 530 540 550
FDFAKSTWEK IEPTKDGPWP SPRYHHSATL VGASLYIFGG AEHKSKYHND
560 570 580 590 600
VYVYKFDANQ WELLNATGET PEPRAGQMTV EWNNSLFTFG GHGGEGGYTS
610 620 630 640 650
FVDAHVFEIA TNTFHEVDCS GTFPRTARPL SYVPYYYGSG DKREGAVFSF
660 670 680 690 700
GGSDGKSPLG SLYQWNLKTH KWKIIKAWMA VEDNTIGSMA AIASGKLDPI
710 720 730
PRYGHCTVLD DTGVISIFGG SGSLFLDDIV QFDMTES
Length:737
Mass (Da):80,955
Last modified:May 5, 2009 - v2
Checksum:iD950BD5BAE6764B0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti506S → G AA sequence (PubMed:8389700).Curated1
Sequence conflicti507T → P AA sequence (PubMed:8389700).Curated1
Sequence conflicti560Q → K AA sequence (PubMed:8896448).Curated1
Sequence conflicti560Q → K AA sequence (PubMed:8389700).Curated1
Sequence conflicti639S → W AA sequence (PubMed:8389700).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U64722 mRNA Translation: AAB08728.1
PIRiS36952
S72442

Entry informationi

Entry nameiAFK_PHYPO
AccessioniPrimary (citable) accession number: P80197
Secondary accession number(s): Q94706
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: May 5, 2009
Last modified: September 12, 2018
This is version 87 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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