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UniProtKB - P80147 (GABT_PIG)
Protein
4-aminobutyrate aminotransferase, mitochondrial
Gene
ABAT
Organism
Sus scrofa (Pig)
Status
Functioni
Catalyzes the conversion of gamma-aminobutyrate and L-beta-aminoisobutyrate to succinate semialdehyde and methylmalonate semialdehyde, respectively. Can also convert delta-aminovalerate and beta-alanine.
By similarityCatalytic activityi
- EC:2.6.1.19By similarityThis reaction proceeds in the forwardBy similarity direction.
- (S)-3-amino-2-methylpropanoate + 2-oxoglutarate = 2-methyl-3-oxopropanoate + L-glutamateBy similarityEC:2.6.1.22By similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
pyridoxal 5'-phosphate2 Publications, [2Fe-2S] cluster1 PublicationNote: Binds 1 [2Fe-2S] cluster per homodimer.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 163 | Iron-sulfur (2Fe-2S); shared with dimeric partner1 Publication | 1 | |
Metal bindingi | 166 | Iron-sulfur (2Fe-2S); shared with dimeric partner1 Publication | 1 | |
Binding sitei | 220 | Substrate1 Publication1 Publication | 1 | |
Binding sitei | 381 | Pyridoxal phosphate; shared with dimeric partner2 Publications | 1 |
GO - Molecular functioni
- (S)-3-amino-2-methylpropionate transaminase activity Source: UniProtKB-EC
- 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
- 4-aminobutyrate:2-oxoglutarate transaminase activity Source: UniProtKB-EC
- identical protein binding Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- protein homodimerization activity Source: UniProtKB
- pyridoxal phosphate binding Source: UniProtKB
- succinate-semialdehyde dehydrogenase binding Source: UniProtKB
GO - Biological processi
- behavioral response to cocaine Source: UniProtKB
- gamma-aminobutyric acid catabolic process Source: UniProtKB
- neurotransmitter catabolic process Source: UniProtKB
Keywordsi
Molecular function | Aminotransferase, Transferase |
Biological process | Neurotransmitter degradation |
Ligand | 2Fe-2S, Iron, Iron-sulfur, Metal-binding, Pyridoxal phosphate |
Enzyme and pathway databases
BRENDAi | 2.6.1.19, 6170 |
SABIO-RKi | P80147 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ABAT Synonyms:GABAT |
Organismi | Sus scrofa (Pig) |
Taxonomic identifieri | 9823 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Suina › Suidae › Sus |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:96910, ABAT |
Subcellular locationi
Mitochondrion
Cytosol
- cytosol Source: UniProtKB
Mitochondrion
- mitochondrial matrix Source: UniProtKB
- mitochondrion Source: UniProtKB
Other locations
- 4-aminobutyrate transaminase complex Source: UniProtKB
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 28 | Mitochondrion1 PublicationAdd BLAST | 28 | |
ChainiPRO_0000001251 | 29 – 500 | 4-aminobutyrate aminotransferase, mitochondrialAdd BLAST | 472 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 231 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 252 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 252 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 279 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 318 | N6-acetyllysineBy similarity | 1 | |
Disulfide bondi | 321 | InterchainBy similarity | ||
Modified residuei | 357 | N6-(pyridoxal phosphate)lysine1 Publication | 1 | |
Modified residuei | 413 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 413 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 452 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 470 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, Disulfide bondProteomic databases
PaxDbi | P80147 |
PeptideAtlasi | P80147 |
PRIDEi | P80147 |
Expressioni
Gene expression databases
ExpressionAtlasi | P80147, baseline and differential |
Genevisiblei | P80147, SS |
Interactioni
Subunit structurei
Homodimer; disulfide-linked.
GO - Molecular functioni
- identical protein binding Source: UniProtKB
- protein homodimerization activity Source: UniProtKB
- succinate-semialdehyde dehydrogenase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 1149627, 1 interactor |
STRINGi | 9823.ENSSSCP00000008445 |
Chemistry databases
BindingDBi | P80147 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P80147 |
SMRi | P80147 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P80147 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 164 – 165 | Pyridoxal phosphate binding1 Publication | 2 |
Sequence similaritiesi
Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1405, Eukaryota |
GeneTreei | ENSGT00550000074885 |
HOGENOMi | CLU_016922_12_1_1 |
InParanoidi | P80147 |
OMAi | DPPDMVT |
OrthoDBi | 566705at2759 |
Family and domain databases
CDDi | cd00610, OAT_like, 1 hit |
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004631, 4NH2But_aminotransferase_euk IPR005814, Aminotrans_3 IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR015422, PyrdxlP-dep_Trfase_small |
Pfami | View protein in Pfam PF00202, Aminotran_3, 1 hit |
PIRSFi | PIRSF000521, Transaminase_4ab_Lys_Orn, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR00699, GABAtrns_euk, 1 hit |
PROSITEi | View protein in PROSITE PS00600, AA_TRANSFER_CLASS_3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
P80147-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASVLLTRRL ACSFRHNHRL LVPGWRHISQ AAAKVDVEFD YDGPLMKTEV
60 70 80 90 100
PGPRSRELMK QLNIIQNAEA VHFFCNYEES RGNYLVDVDG NRMLDLYSQI
110 120 130 140 150
SSIPIGYSHP ALVKLVQQPQ NVSTFINRPA LGILPPENFV EKLRESLLSV
160 170 180 190 200
APKGMSQLIT MACGSCSNEN AFKTIFMWYR SKERGQSAFS KEELETCMIN
210 220 230 240 250
QAPGCPDYSI LSFMGAFHGR TMGCLATTHS KAIHKIDIPS FDWPIAPFPR
260 270 280 290 300
LKYPLEEFVK ENQQEEARCL EEVEDLIVKY RKKKKTVAGI IVEPIQSEGG
310 320 330 340 350
DNHASDDFFR KLRDISRKHG CAFLVDEVQT GGGSTGKFWA HEHWGLDDPA
360 370 380 390 400
DVMTFSKKMM TGGFFHKEEF RPNAPYRIFN TWLGDPSKNL LLAEVINIIK
410 420 430 440 450
REDLLSNAAH AGKVLLTGLL DLQARYPQFI SRVRGRGTFC SFDTPDESIR
460 470 480 490 500
NKLISIARNK GVMLGGCGDK SIRFRPTLVF RDHHAHLFLN IFSDILADFK
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A5S6H7P5 | A0A5S6H7P5_PIG | 4-aminobutyrate aminotransferase, m... | ABAT | 520 | Annotation score: | ||
A0A286ZMM3 | A0A286ZMM3_PIG | 4-aminobutyrate aminotransferase, m... | ABAT | 490 | Annotation score: | ||
A0A5S6H1Z0 | A0A5S6H1Z0_PIG | 4-aminobutyrate aminotransferase, m... | ABAT | 451 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 454 | I → N in AAA96981 (PubMed:1559966).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M84802 mRNA Translation: AAA96981.1 |
RefSeqi | NP_999428.1, NM_214263.1 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M84802 mRNA Translation: AAA96981.1 |
RefSeqi | NP_999428.1, NM_214263.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1OHV | X-ray | 2.30 | A/B/C/D | 29-500 | [»] | |
1OHW | X-ray | 2.30 | A/B/C/D | 29-500 | [»] | |
1OHY | X-ray | 2.80 | A/B/C/D | 29-500 | [»] | |
4Y0D | X-ray | 2.19 | A/B/C/D | 39-500 | [»] | |
4Y0H | X-ray | 1.63 | A/B/C/D | 39-500 | [»] | |
4Y0I | X-ray | 1.66 | A/B/C/D | 39-499 | [»] | |
4ZSW | X-ray | 1.70 | A/B/C/D | 39-499 | [»] | |
4ZSY | X-ray | 1.70 | A/B/C/D | 39-499 | [»] | |
6B6G | X-ray | 1.95 | A/B/C/D | 39-500 | [»] | |
AlphaFoldDBi | P80147 | |||||
SMRi | P80147 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 1149627, 1 interactor |
STRINGi | 9823.ENSSSCP00000008445 |
Chemistry databases
BindingDBi | P80147 |
ChEMBLi | CHEMBL2266 |
Proteomic databases
PaxDbi | P80147 |
PeptideAtlasi | P80147 |
PRIDEi | P80147 |
Genome annotation databases
Organism-specific databases
CTDi | 18 |
VGNCi | VGNC:96910, ABAT |
Phylogenomic databases
eggNOGi | KOG1405, Eukaryota |
GeneTreei | ENSGT00550000074885 |
HOGENOMi | CLU_016922_12_1_1 |
InParanoidi | P80147 |
OMAi | DPPDMVT |
OrthoDBi | 566705at2759 |
Enzyme and pathway databases
BRENDAi | 2.6.1.19, 6170 |
SABIO-RKi | P80147 |
Miscellaneous databases
ChiTaRSi | ABAT, pig |
EvolutionaryTracei | P80147 |
PROi | PR:P80147 |
Gene expression databases
ExpressionAtlasi | P80147, baseline and differential |
Genevisiblei | P80147, SS |
Family and domain databases
CDDi | cd00610, OAT_like, 1 hit |
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004631, 4NH2But_aminotransferase_euk IPR005814, Aminotrans_3 IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR015422, PyrdxlP-dep_Trfase_small |
Pfami | View protein in Pfam PF00202, Aminotran_3, 1 hit |
PIRSFi | PIRSF000521, Transaminase_4ab_Lys_Orn, 1 hit |
SUPFAMi | SSF53383, SSF53383, 1 hit |
TIGRFAMsi | TIGR00699, GABAtrns_euk, 1 hit |
PROSITEi | View protein in PROSITE PS00600, AA_TRANSFER_CLASS_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | GABT_PIG | |
Accessioni | P80147Primary (citable) accession number: P80147 Secondary accession number(s): P27820 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | December 1, 1992 | |
Last modified: | May 25, 2022 | |
This is version 161 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families