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Protein

Phosphatidylinositol-glycan-specific phospholipase D

Gene

GPLD1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane.

Catalytic activityi

6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O = 6-(alpha-D-glucosaminyl)-1D-myo-inositol + phosphatidate.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BRENDAi3.1.4.50 2681
ReactomeiR-HSA-163125 Post-translational modification: synthesis of GPI-anchored proteins
SignaLinkiP80108

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol-glycan-specific phospholipase D (EC:3.1.4.50)
Short name:
PI-G PLD
Alternative name(s):
Glycoprotein phospholipase D
Glycosyl-phosphatidylinositol-specific phospholipase D
Short name:
GPI-PLD
Short name:
GPI-specific phospholipase D
Gene namesi
Name:GPLD1
Synonyms:PIGPLD1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000112293.14
HGNCiHGNC:4459 GPLD1
MIMi602515 gene
neXtProtiNX_P80108

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi2822
OpenTargetsiENSG00000112293
PharmGKBiPA28842

Polymorphism and mutation databases

BioMutaiGPLD1
DMDMi126302583

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Add BLAST23
ChainiPRO_000002204724 – 840Phosphatidylinositol-glycan-specific phospholipase DAdd BLAST817

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi94N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi271N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi292N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi307N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi321N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi501N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi568N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi591N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi604N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi659N-linked (GlcNAc...) asparagine1 Publication1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP80108
PeptideAtlasiP80108
PRIDEiP80108
ProteomicsDBi57666
57667 [P80108-2]

PTM databases

GlyConnecti1601
iPTMnetiP80108
PhosphoSitePlusiP80108

Expressioni

Gene expression databases

BgeeiENSG00000112293 Expressed in 165 organ(s), highest expression level in secondary oocyte
GenevisibleiP80108 HS

Organism-specific databases

HPAiCAB008625
HPA012500

Interactioni

Subunit structurei

Monomer.Curated

Protein-protein interaction databases

BioGridi109083, 6 interactors
STRINGi9606.ENSP00000230036

Structurei

3D structure databases

ProteinModelPortaliP80108
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati367 – 428FG-GAP 1PROSITE-ProRule annotationAdd BLAST62
Repeati436 – 497FG-GAP 2PROSITE-ProRule annotationAdd BLAST62
Repeati499 – 559FG-GAP 3PROSITE-ProRule annotationAdd BLAST61
Repeati563 – 623FG-GAP 4PROSITE-ProRule annotationAdd BLAST61
Repeati633 – 693FG-GAP 5PROSITE-ProRule annotationAdd BLAST61
Repeati704 – 770FG-GAP 6PROSITE-ProRule annotationAdd BLAST67
Repeati788 – 840FG-GAP 7PROSITE-ProRule annotationAdd BLAST53

Sequence similaritiesi

Belongs to the GPLD1 family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IPB3 Eukaryota
ENOG4111ZDA LUCA
GeneTreeiENSGT00390000013522
HOVERGENiHBG008185
InParanoidiP80108
KOiK01127
OMAiAQYVLIS
OrthoDBiEOG091G01DS
PhylomeDBiP80108
TreeFamiTF335726

Family and domain databases

Gene3Di2.130.10.130, 2 hits
InterProiView protein in InterPro
IPR013517 FG-GAP
IPR001028 Gprt_PLipase_D
IPR013519 Int_alpha_beta-p
IPR028994 Integrin_alpha_N
IPR029002 PLPC/GPLD1
PfamiView protein in Pfam
PF01839 FG-GAP, 3 hits
PF00882 Zn_dep_PLPC, 1 hit
PRINTSiPR00718 PHPHLIPASED
SMARTiView protein in SMART
SM00191 Int_alpha, 5 hits
PROSITEiView protein in PROSITE
PS51470 FG_GAP, 7 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: P80108-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSAFRLWPGL LIMLGSLCHR GSPCGLSTHV EIGHRALEFL QLHNGRVNYR
60 70 80 90 100
ELLLEHQDAY QAGIVFPDCF YPSICKGGKF HDVSESTHWT PFLNASVHYI
110 120 130 140 150
RENYPLPWEK DTEKLVAFLF GITSHMAADV SWHSLGLEQG FLRTMGAIDF
160 170 180 190 200
HGSYSEAHSA GDFGGDVLSQ FEFNFNYLAR RWYVPVKDLL GIYEKLYGRK
210 220 230 240 250
VITENVIVDC SHIQFLEMYG EMLAVSKLYP TYSTKSPFLV EQFQEYFLGG
260 270 280 290 300
LDDMAFWSTN IYHLTSFMLE NGTSDCNLPE NPLFIACGGQ QNHTQGSKMQ
310 320 330 340 350
KNDFHRNLTT SLTESVDRNI NYTERGVFFS VNSWTPDSMS FIYKALERNI
360 370 380 390 400
RTMFIGGSQL SQKHVSSPLA SYFLSFPYAR LGWAMTSADL NQDGHGDLVV
410 420 430 440 450
GAPGYSRPGH IHIGRVYLIY GNDLGLPPVD LDLDKEAHRI LEGFQPSGRF
460 470 480 490 500
GSALAVLDFN VDGVPDLAVG APSVGSEQLT YKGAVYVYFG SKQGGMSSSP
510 520 530 540 550
NITISCQDIY CNLGWTLLAA DVNGDSEPDL VIGSPFAPGG GKQKGIVAAF
560 570 580 590 600
YSGPSLSDKE KLNVEAANWT VRGEEDFSWF GYSLHGVTVD NRTLLLVGSP
610 620 630 640 650
TWKNASRLGH LLHIRDEKKS LGRVYGYFPP NGQSWFTISG DKAMGKLGTS
660 670 680 690 700
LSSGHVLMNG TLKQVLLVGA PTYDDVSKVA FLTVTLHQGG ATRMYALTSD
710 720 730 740 750
AQPLLLSTFS GDRRFSRFGG VLHLSDLDDD GLDEIIMAAP LRIADVTSGL
760 770 780 790 800
IGGEDGRVYV YNGKETTLGD MTGKCKSWIT PCPEEKAQYV LISPEASSRF
810 820 830 840
GSSLITVRSK AKNQVVIAAG RSSLGARLSG ALHVYSLGSD
Length:840
Mass (Da):92,336
Last modified:February 20, 2007 - v3
Checksum:i11369BAFEC3D6D38
GO
Isoform 2 (identifier: P80108-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     165-176: GDVLSQFEFNFN → TVYLHLLNFLVV
     177-840: Missing.

Show »
Length:176
Mass (Da):19,865
Checksum:iB71070D7DD58234B
GO

Sequence cautioni

The sequence AAA36444 differs from that shown. This sequence has numerous of conflicts with the human genome.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti531 – 534VIGS → MLGT AA sequence (PubMed:1606959).Curated4

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03074317L → V1 PublicationCorresponds to variant dbSNP:rs2235501Ensembl.1
Natural variantiVAR_03074430V → I3 PublicationsCorresponds to variant dbSNP:rs1126617Ensembl.1
Natural variantiVAR_030745275D → E. Corresponds to variant dbSNP:rs17300770Ensembl.1
Natural variantiVAR_051278350I → V1 PublicationCorresponds to variant dbSNP:rs1062496Ensembl.1
Natural variantiVAR_030746396G → S. Corresponds to variant dbSNP:rs6924628Ensembl.1
Natural variantiVAR_030747461V → M1 PublicationCorresponds to variant dbSNP:rs1062505Ensembl.1
Natural variantiVAR_030748694M → V1 PublicationCorresponds to variant dbSNP:rs1042303Ensembl.1
Natural variantiVAR_030749698T → I4 PublicationsCorresponds to variant dbSNP:rs1772256Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_023261165 – 176GDVLS…EFNFN → TVYLHLLNFLVV in isoform 2. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_023262177 – 840Missing in isoform 2. 1 PublicationAdd BLAST664

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11701 mRNA Translation: AAA36444.1 Sequence problems.
L11702 mRNA Translation: AAA36445.1
AJ308108 mRNA Translation: CAC87068.1
AJ400872
, AJ400873, AJ400874, AJ400875, AJ400876 Genomic DNA Translation: CAC14844.1
AY007546 mRNA Translation: AAG16627.2
AL359713, AL031230 Genomic DNA Translation: CAI17103.1
AL031230, AL359713 Genomic DNA Translation: CAI22602.1
AL031230 Genomic DNA Translation: CAD92520.1
BC007614 mRNA Translation: AAH07614.1
BC020748 mRNA Translation: AAH20748.1
BC093645 mRNA Translation: AAH93645.1
BC112001 mRNA Translation: AAI12002.1
CCDSiCCDS4553.1 [P80108-1]
RefSeqiNP_001494.2, NM_001503.3 [P80108-1]
UniGeneiHs.533291

Genome annotation databases

EnsembliENST00000230036; ENSP00000230036; ENSG00000112293 [P80108-1]
GeneIDi2822
KEGGihsa:2822
UCSCiuc003ned.3 human [P80108-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L11701 mRNA Translation: AAA36444.1 Sequence problems.
L11702 mRNA Translation: AAA36445.1
AJ308108 mRNA Translation: CAC87068.1
AJ400872
, AJ400873, AJ400874, AJ400875, AJ400876 Genomic DNA Translation: CAC14844.1
AY007546 mRNA Translation: AAG16627.2
AL359713, AL031230 Genomic DNA Translation: CAI17103.1
AL031230, AL359713 Genomic DNA Translation: CAI22602.1
AL031230 Genomic DNA Translation: CAD92520.1
BC007614 mRNA Translation: AAH07614.1
BC020748 mRNA Translation: AAH20748.1
BC093645 mRNA Translation: AAH93645.1
BC112001 mRNA Translation: AAI12002.1
CCDSiCCDS4553.1 [P80108-1]
RefSeqiNP_001494.2, NM_001503.3 [P80108-1]
UniGeneiHs.533291

3D structure databases

ProteinModelPortaliP80108
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109083, 6 interactors
STRINGi9606.ENSP00000230036

PTM databases

GlyConnecti1601
iPTMnetiP80108
PhosphoSitePlusiP80108

Polymorphism and mutation databases

BioMutaiGPLD1
DMDMi126302583

Proteomic databases

PaxDbiP80108
PeptideAtlasiP80108
PRIDEiP80108
ProteomicsDBi57666
57667 [P80108-2]

Protocols and materials databases

DNASUi2822
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000230036; ENSP00000230036; ENSG00000112293 [P80108-1]
GeneIDi2822
KEGGihsa:2822
UCSCiuc003ned.3 human [P80108-1]

Organism-specific databases

CTDi2822
DisGeNETi2822
EuPathDBiHostDB:ENSG00000112293.14
GeneCardsiGPLD1
H-InvDBiHIX0032817
HGNCiHGNC:4459 GPLD1
HPAiCAB008625
HPA012500
MIMi602515 gene
neXtProtiNX_P80108
OpenTargetsiENSG00000112293
PharmGKBiPA28842
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IPB3 Eukaryota
ENOG4111ZDA LUCA
GeneTreeiENSGT00390000013522
HOVERGENiHBG008185
InParanoidiP80108
KOiK01127
OMAiAQYVLIS
OrthoDBiEOG091G01DS
PhylomeDBiP80108
TreeFamiTF335726

Enzyme and pathway databases

BRENDAi3.1.4.50 2681
ReactomeiR-HSA-163125 Post-translational modification: synthesis of GPI-anchored proteins
SignaLinkiP80108

Miscellaneous databases

GeneWikiiGPLD1
GenomeRNAii2822
PROiPR:P80108
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000112293 Expressed in 165 organ(s), highest expression level in secondary oocyte
GenevisibleiP80108 HS

Family and domain databases

Gene3Di2.130.10.130, 2 hits
InterProiView protein in InterPro
IPR013517 FG-GAP
IPR001028 Gprt_PLipase_D
IPR013519 Int_alpha_beta-p
IPR028994 Integrin_alpha_N
IPR029002 PLPC/GPLD1
PfamiView protein in Pfam
PF01839 FG-GAP, 3 hits
PF00882 Zn_dep_PLPC, 1 hit
PRINTSiPR00718 PHPHLIPASED
SMARTiView protein in SMART
SM00191 Int_alpha, 5 hits
PROSITEiView protein in PROSITE
PS51470 FG_GAP, 7 hits
ProtoNetiSearch...

Entry informationi

Entry nameiPHLD_HUMAN
AccessioniPrimary (citable) accession number: P80108
Secondary accession number(s): Q15127
, Q15128, Q2M2F2, Q5T3Y0, Q7Z6T8, Q8TCV0, Q8WW82, Q96ID6, Q9H167, Q9H4M1, Q9UJC9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: February 20, 2007
Last modified: October 10, 2018
This is version 153 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. SIMILARITY comments
    Index of protein domains and families
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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