UniProtKB - P80078 (GMSS_CLOCO)
Protein
Glutamate mutase sigma subunit
Gene
glmS
Organism
Clostridium cochlearium
Status
Functioni
Catalyzes the carbon skeleton rearrangement of L-glutamate to L-threo-3-methylaspartate ((2S,3S)-3-methylaspartate).UniRule annotation3 Publications
Catalytic activityi
- EC:5.4.99.1UniRule annotation2 Publications
Cofactori
adenosylcob(III)alaminUniRule annotation5 Publications
Activity regulationi
Competitively inhibited by (2S,4S)-4-fluoroglutamate, 2-methyleneglutarate, (2R,3RS)-3-fluoroglutamate and (S)-3-methylitaconate.2 Publications
: L-glutamate degradation via mesaconate pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes acetate and pyruvate from L-glutamate.UniRule annotationProteins known to be involved in the 4 steps of the subpathway in this organism are:
- Glutamate mutase epsilon subunit (mutE), Glutamate mutase epsilon subunit (glmE), Glutamate mutase sigma subunit (glmS), Glutamate mutase sigma subunit (mutS_3), Glutamate mutase epsilon subunit (glmE), Glutamate mutase sigma subunit (mutS_3), Glutamate mutase epsilon subunit (mutE)
- Methylaspartate ammonia-lyase (NCTC13028_02262)
- no protein annotated in this organism
- no protein annotated in this organism
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetate and pyruvate from L-glutamate, the pathway L-glutamate degradation via mesaconate pathway and in Amino-acid degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 16 | Cobalt (cobalamin axial ligand) | 1 |
GO - Molecular functioni
- cobalamin binding Source: UniProtKB-KW
- metal ion binding Source: UniProtKB-KW
- methylaspartate mutase activity Source: UniProtKB-UniRule
GO - Biological processi
- anaerobic glutamate catabolic process Source: UniProtKB-UniRule
- glutamate catabolic process via L-citramalate Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Isomerase |
Ligand | Cobalamin, Cobalt, Metal-binding |
Enzyme and pathway databases
BRENDAi | 5.4.99.1, 1471 |
SABIO-RKi | P80078 |
UniPathwayi | UPA00561;UER00617 |
Names & Taxonomyi
Protein namesi | Recommended name: Glutamate mutase sigma subunitUniRule annotation (EC:5.4.99.1UniRule annotation)Alternative name(s): Glutamate mutase S chainUniRule annotation Glutamate mutase small subunitUniRule annotation Methylaspartate mutaseUniRule annotation |
Gene namesi | Name:glmSUniRule annotation |
Organismi | Clostridium cochlearium |
Taxonomic identifieri | 1494 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000216443 | 1 – 137 | Glutamate mutase sigma subunitAdd BLAST | 137 |
Proteomic databases
PRIDEi | P80078 |
Interactioni
Subunit structurei
Heterotetramer composed of 2 epsilon subunits (GlmE) and 2 sigma subunits (GlmS). GlmE exists as a homodimer and GlmS as a monomer.
UniRule annotation5 PublicationsBinary interactionsi
P80078
With | #Exp. | IntAct |
---|---|---|
glmE [P80077] | 2 | EBI-1028147,EBI-1028142 |
Protein-protein interaction databases
IntActi | P80078, 1 interactor |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P80078 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P80078 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 3 – 137 | B12-bindingUniRule annotationAdd BLAST | 135 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 13 – 17 | Adenosylcobalamin binding | 5 | |
Regioni | 61 – 63 | Adenosylcobalamin binding | 3 | |
Regioni | 93 – 97 | Adenosylcobalamin binding | 5 |
Sequence similaritiesi
Belongs to the methylaspartate mutase GlmS subunit family.UniRule annotation
Family and domain databases
CDDi | cd02072, Glm_B12_BD, 1 hit |
HAMAPi | MF_00526, Me_Asp_mutase_S, 1 hit |
InterProi | View protein in InterPro IPR006158, Cobalamin-bd IPR036724, Cobalamin-bd_sf IPR006394, GlmS |
Pfami | View protein in Pfam PF02310, B12-binding, 1 hit |
SUPFAMi | SSF52242, SSF52242, 1 hit |
TIGRFAMsi | TIGR01501, MthylAspMutase, 1 hit |
PROSITEi | View protein in PROSITE PS51332, B12_BINDING, 1 hit |
i Sequence
Sequence statusi: Complete.
P80078-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEKKTIVLGV IGSDCHAVGN KILDHAFTNA GFNVVNIGVL SPQEVFIKAA
60 70 80 90 100
IETKADAILL SSLYGQGEID CKGLRQKCDE AGLEGILLYV GGNIVVGKQH
110 120 130
WPDVEKRFKD MGYDRVYAPG TPPEVGIADL KKDLNIE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X80997 Genomic DNA Translation: CAA56921.1 X75890 Genomic DNA Translation: CAA53484.1 |
PIRi | I40658 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X80997 Genomic DNA Translation: CAA56921.1 X75890 Genomic DNA Translation: CAA53484.1 |
PIRi | I40658 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1B1A | NMR | - | A | 1-137 | [»] | |
1CB7 | X-ray | 2.00 | A/C | 1-137 | [»] | |
1CCW | X-ray | 1.60 | A/C | 1-137 | [»] | |
1I9C | X-ray | 1.90 | A/C | 1-137 | [»] | |
6H9E | X-ray | 1.82 | A/C | 1-137 | [»] | |
6H9F | X-ray | 2.10 | A/C | 1-137 | [»] | |
SMRi | P80078 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P80078, 1 interactor |
Proteomic databases
PRIDEi | P80078 |
Enzyme and pathway databases
UniPathwayi | UPA00561;UER00617 |
BRENDAi | 5.4.99.1, 1471 |
SABIO-RKi | P80078 |
Miscellaneous databases
EvolutionaryTracei | P80078 |
Family and domain databases
CDDi | cd02072, Glm_B12_BD, 1 hit |
HAMAPi | MF_00526, Me_Asp_mutase_S, 1 hit |
InterProi | View protein in InterPro IPR006158, Cobalamin-bd IPR036724, Cobalamin-bd_sf IPR006394, GlmS |
Pfami | View protein in Pfam PF02310, B12-binding, 1 hit |
SUPFAMi | SSF52242, SSF52242, 1 hit |
TIGRFAMsi | TIGR01501, MthylAspMutase, 1 hit |
PROSITEi | View protein in PROSITE PS51332, B12_BINDING, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GMSS_CLOCO | |
Accessioni | P80078Primary (citable) accession number: P80078 Secondary accession number(s): Q60144 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 1, 1992 |
Last sequence update: | December 13, 2001 | |
Last modified: | October 7, 2020 | |
This is version 107 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families