UniProtKB - P80067 (CATC_RAT)
Protein
Dipeptidyl peptidase 1
Gene
Ctsc
Organism
Rattus norvegicus (Rat)
Status
Functioni
Thiol protease. Has dipeptidylpeptidase activity. Can act as both an exopeptidase and endopeptidase (By similarity).By similarity
Catalytic activityi
- Release of an N-terminal dipeptide, Xaa-Yaa-|-Zaa-, except when Xaa is Arg or Lys, or Yaa or Zaa is Pro. EC:3.4.14.1
Cofactori
chlorideBy similarityNote: Binds 1 Cl- ion per heavy chain.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 257 | By similarity | 1 | |
Binding sitei | 301 | ChlorideBy similarity | 1 | |
Binding sitei | 303 | Chloride; via amide nitrogenBy similarity | 1 | |
Binding sitei | 346 | ChlorideBy similarity | 1 | |
Active sitei | 404 | By similarity | 1 | |
Active sitei | 426 | By similarity | 1 |
GO - Molecular functioni
- chaperone binding Source: ParkinsonsUK-UCL
- chloride ion binding Source: RGD
- cysteine-type endopeptidase activity Source: GO_Central
- cysteine-type peptidase activity Source: RGD
- identical protein binding Source: RGD
- peptidase activator activity involved in apoptotic process Source: RGD
- phosphatase binding Source: ParkinsonsUK-UCL
- protein self-association Source: RGD
- serine-type endopeptidase activity Source: RGD
GO - Biological processi
- aging Source: RGD
- negative regulation of myelination Source: RGD
- positive regulation of apoptotic signaling pathway Source: RGD
- positive regulation of microglial cell activation Source: RGD
- positive regulation of proteolysis involved in cellular protein catabolic process Source: ParkinsonsUK-UCL
- proteolysis Source: RGD
- proteolysis involved in cellular protein catabolic process Source: GO_Central
- response to organic substance Source: RGD
- T cell mediated cytotoxicity Source: RGD
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Ligand | Chloride |
Enzyme and pathway databases
Reactomei | R-RNO-204005, COPII-mediated vesicle transport R-RNO-2132295, MHC class II antigen presentation R-RNO-5694530, Cargo concentration in the ER R-RNO-6798695, Neutrophil degranulation |
SABIO-RKi | P80067 |
Protein family/group databases
MEROPSi | C01.070 |
Names & Taxonomyi
Protein namesi | Recommended name: Dipeptidyl peptidase 1 (EC:3.4.14.1)Alternative name(s): Cathepsin C Cathepsin J Dipeptidyl peptidase I Short name: DPP-I Short name: DPPI Dipeptidyl transferase Cleaved into the following 3 chains: Alternative name(s): Dipeptidyl peptidase I exclusion domain chain Alternative name(s): Dipeptidyl peptidase I heavy chain Alternative name(s): Dipeptidyl peptidase I light chain |
Gene namesi | Name:Ctsc |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2445, Ctsc |
Subcellular locationi
Lysosome
Cytoskeleton
- centrosome Source: Ensembl
Endoplasmic reticulum
- endoplasmic reticulum Source: RGD
Extracellular region or secreted
- extracellular space Source: GO_Central
Golgi apparatus
- Golgi apparatus Source: RGD
Lysosome
- lysosome Source: ParkinsonsUK-UCL
Nucleus
- nucleoplasm Source: Ensembl
Other locations
- cytoplasm Source: RGD
Keywords - Cellular componenti
LysosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | 1 PublicationAdd BLAST | 24 | |
ChainiPRO_0000026350 | 25 – 134 | Dipeptidyl peptidase 1 exclusion domain chainBy similarityAdd BLAST | 110 | |
PropeptideiPRO_0000026351 | 135 – 229 | By similarityAdd BLAST | 95 | |
ChainiPRO_0000026352 | 230 – 393 | Dipeptidyl peptidase 1 heavy chainAdd BLAST | 164 | |
ChainiPRO_0000026353 | 394 – 462 | Dipeptidyl peptidase 1 light chainAdd BLAST | 69 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 29 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 30 ↔ 118 | 1 Publication | ||
Glycosylationi | 53 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 54 ↔ 136 | 1 Publication | ||
Glycosylationi | 144 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 254 ↔ 297 | 1 Publication | ||
Glycosylationi | 275 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 290 ↔ 330 | 1 Publication | ||
Disulfide bondi | 320 ↔ 336 | 1 Publication |
Post-translational modificationi
N-glycosylated.1 Publication
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
PaxDbi | P80067 |
PRIDEi | P80067 |
PTM databases
GlyGeni | P80067, 4 sites |
iPTMneti | P80067 |
PhosphoSitePlusi | P80067 |
Expressioni
Tissue specificityi
Broadly distributed, but higher levels found in liver, spleen, intestine, lung and kidney.
Gene expression databases
Bgeei | ENSRNOG00000016496, Expressed in liver and 21 other tissues |
Genevisiblei | P80067, RN |
Interactioni
Subunit structurei
Tetramer of heterotrimers consisting of exclusion domain, heavy- and light chains.
1 PublicationGO - Molecular functioni
- chaperone binding Source: ParkinsonsUK-UCL
- identical protein binding Source: RGD
- phosphatase binding Source: ParkinsonsUK-UCL
- protein self-association Source: RGD
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000022342 |
Chemistry databases
BindingDBi | P80067 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P80067 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P80067 |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase C1 family.PROSITE-ProRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG1543, Eukaryota |
GeneTreei | ENSGT00940000155787 |
HOGENOMi | CLU_048219_0_0_1 |
InParanoidi | P80067 |
OMAi | FVTPVRN |
OrthoDBi | 1275401at2759 |
PhylomeDBi | P80067 |
TreeFami | TF313225 |
Family and domain databases
CDDi | cd02621, Peptidase_C1A_CathepsinC, 1 hit |
Gene3Di | 2.40.128.80, 1 hit |
InterProi | View protein in InterPro IPR039412, CatC IPR014882, CathepsinC_exc IPR036496, CathepsinC_exc_dom_sf IPR038765, Papain-like_cys_pep_sf IPR025661, Pept_asp_AS IPR000169, Pept_cys_AS IPR025660, Pept_his_AS IPR000668, Peptidase_C1A_C |
Pfami | View protein in Pfam PF08773, CathepsinC_exc, 1 hit PF00112, Peptidase_C1, 1 hit |
PRINTSi | PR00705, PAPAIN |
SMARTi | View protein in SMART SM00645, Pept_C1, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit SSF75001, SSF75001, 1 hit |
PROSITEi | View protein in PROSITE PS00640, THIOL_PROTEASE_ASN, 1 hit PS00139, THIOL_PROTEASE_CYS, 1 hit PS00639, THIOL_PROTEASE_HIS, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P80067-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGPWTHSLRA ALLLVLLGVC TVSSDTPANC TYPDLLGTWV FQVGPRHPRS
60 70 80 90 100
HINCSVMEPT EEKVVIHLKK LDTAYDEVGN SGYFTLIYNQ GFEIVLNDYK
110 120 130 140 150
WFAFFKYEVK GSRAISYCHE TMTGWVHDVL GRNWACFVGK KMANHSEKVY
160 170 180 190 200
VNVAHLGGLQ EKYSERLYSH NHNFVKAINS VQKSWTATTY EEYEKLSIRD
210 220 230 240 250
LIRRSGHSGR ILRPKPAPIT DEIQQQILSL PESWDWRNVR GINFVSPVRN
260 270 280 290 300
QESCGSCYSF ASLGMLEARI RILTNNSQTP ILSPQEVVSC SPYAQGCDGG
310 320 330 340 350
FPYLIAGKYA QDFGVVEENC FPYTATDAPC KPKENCLRYY SSEYYYVGGF
360 370 380 390 400
YGGCNEALMK LELVKHGPMA VAFEVHDDFL HYHSGIYHHT GLSDPFNPFE
410 420 430 440 450
LTNHAVLLVG YGKDPVTGLD YWIVKNSWGS QWGESGYFRI RRGTDECAIE
460
SIAMAAIPIP KL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 30 | C → E AA sequence (PubMed:1740150).Curated | 1 | |
Sequence conflicti | 129 | V → Y (PubMed:1515062).Curated | 1 | |
Sequence conflicti | 171 | N → H (PubMed:1515062).Curated | 1 | |
Sequence conflicti | 191 – 192 | EE → RR (PubMed:1515062).Curated | 2 | |
Sequence conflicti | 263 | L → I (PubMed:1515062).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D90404 mRNA Translation: BAA14400.1 |
PIRi | A41158 |
RefSeqi | NP_058793.1, NM_017097.1 |
Genome annotation databases
Ensembli | ENSRNOT00000022342; ENSRNOP00000022342; ENSRNOG00000016496 |
GeneIDi | 25423 |
KEGGi | rno:25423 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D90404 mRNA Translation: BAA14400.1 |
PIRi | A41158 |
RefSeqi | NP_058793.1, NM_017097.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1JQP | X-ray | 2.40 | A | 25-462 | [»] | |
SMRi | P80067 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000022342 |
Chemistry databases
BindingDBi | P80067 |
ChEMBLi | CHEMBL3308944 |
GuidetoPHARMACOLOGYi | 2344 |
Protein family/group databases
MEROPSi | C01.070 |
PTM databases
GlyGeni | P80067, 4 sites |
iPTMneti | P80067 |
PhosphoSitePlusi | P80067 |
Proteomic databases
PaxDbi | P80067 |
PRIDEi | P80067 |
Genome annotation databases
Ensembli | ENSRNOT00000022342; ENSRNOP00000022342; ENSRNOG00000016496 |
GeneIDi | 25423 |
KEGGi | rno:25423 |
Organism-specific databases
CTDi | 1075 |
RGDi | 2445, Ctsc |
Phylogenomic databases
eggNOGi | KOG1543, Eukaryota |
GeneTreei | ENSGT00940000155787 |
HOGENOMi | CLU_048219_0_0_1 |
InParanoidi | P80067 |
OMAi | FVTPVRN |
OrthoDBi | 1275401at2759 |
PhylomeDBi | P80067 |
TreeFami | TF313225 |
Enzyme and pathway databases
Reactomei | R-RNO-204005, COPII-mediated vesicle transport R-RNO-2132295, MHC class II antigen presentation R-RNO-5694530, Cargo concentration in the ER R-RNO-6798695, Neutrophil degranulation |
SABIO-RKi | P80067 |
Miscellaneous databases
EvolutionaryTracei | P80067 |
PROi | PR:P80067 |
Gene expression databases
Bgeei | ENSRNOG00000016496, Expressed in liver and 21 other tissues |
Genevisiblei | P80067, RN |
Family and domain databases
CDDi | cd02621, Peptidase_C1A_CathepsinC, 1 hit |
Gene3Di | 2.40.128.80, 1 hit |
InterProi | View protein in InterPro IPR039412, CatC IPR014882, CathepsinC_exc IPR036496, CathepsinC_exc_dom_sf IPR038765, Papain-like_cys_pep_sf IPR025661, Pept_asp_AS IPR000169, Pept_cys_AS IPR025660, Pept_his_AS IPR000668, Peptidase_C1A_C |
Pfami | View protein in Pfam PF08773, CathepsinC_exc, 1 hit PF00112, Peptidase_C1, 1 hit |
PRINTSi | PR00705, PAPAIN |
SMARTi | View protein in SMART SM00645, Pept_C1, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit SSF75001, SSF75001, 1 hit |
PROSITEi | View protein in PROSITE PS00640, THIOL_PROTEASE_ASN, 1 hit PS00139, THIOL_PROTEASE_CYS, 1 hit PS00639, THIOL_PROTEASE_HIS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CATC_RAT | |
Accessioni | P80067Primary (citable) accession number: P80067 Secondary accession number(s): P80068 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 1992 |
Last sequence update: | September 5, 2006 | |
Last modified: | December 2, 2020 | |
This is version 172 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries