UniProtKB - P79171 (AMPN_FELCA)
Aminopeptidase N
ANPEP
Functioni
Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. May have a role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity (By similarity).
By similarity(Microbial infection) In case of feline coronavirus (FCoV) infection, serves as a receptor for FCoV spike glycoprotein. It is as well a receptor for other serogroup I coronaviruses, like canine coronavirus (CCoV), porcine transmissible gastroenteritis virus (TGEV), and human coronavirus 229E (HCoV-229E). Also serves as a receptor for infectious bronchitis virus (IBV, Arkansas 99 serotype) in serogroup III.
3 PublicationsCatalytic activityi
- Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.By similarity EC:3.4.11.2
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 387 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Active sitei | 388 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Metal bindingi | 391 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Metal bindingi | 410 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Sitei | 476 | Transition state stabilizerBy similarity | 1 |
GO - Molecular functioni
- metalloaminopeptidase activity Source: GO_Central
- peptide binding Source: GO_Central
- virus receptor activity Source: UniProtKB-KW
- zinc ion binding Source: GO_Central
GO - Biological processi
- angiogenesis Source: UniProtKB-KW
- cell differentiation Source: UniProtKB-KW
- peptide catabolic process Source: GO_Central
- proteolysis Source: GO_Central
- regulation of blood pressure Source: GO_Central
- signal transduction Source: GO_Central
Keywordsi
Molecular function | Aminopeptidase, Developmental protein, Host cell receptor for virus entry, Hydrolase, Metalloprotease, Protease, Receptor |
Biological process | Angiogenesis, Differentiation, Host-virus interaction |
Ligand | Metal-binding, Zinc |
Protein family/group databases
MEROPSi | M01.001 |
Names & Taxonomyi
Protein namesi | Recommended name: Aminopeptidase NCurated (EC:3.4.11.2By similarity)Short name: AP-N Short name: fAPN Alternative name(s): Alanyl aminopeptidase Aminopeptidase M Short name: AP-M Microsomal aminopeptidase CD_antigen: CD13 |
Gene namesi | Name:ANPEP Synonyms:APN |
Organismi | Felis catus (Cat) (Felis silvestris catus) |
Taxonomic identifieri | 9685 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Feliformia › Felidae › Felinae › Felis |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane 2 Publications; Single-pass type II membrane protein 2 Publications
Note: Also found as a soluble form.By similarity
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 8 | Cytoplasmic1 Publication | 8 | |
Transmembranei | 9 – 32 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 24 | |
Topological domaini | 33 – 967 | Extracellular2 PublicationsAdd BLAST | 935 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000095080 | 1 – 967 | Aminopeptidase NAdd BLAST | 967 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 38 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 84 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 126 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 175 | SulfotyrosineSequence analysis | 1 | |
Glycosylationi | 233 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 338 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 418 | SulfotyrosineSequence analysis | 1 | |
Glycosylationi | 626 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 682 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 740 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 762 ↔ 769 | By similarity | ||
Disulfide bondi | 799 ↔ 835 | By similarity |
Post-translational modificationi
Keywords - PTMi
Disulfide bond, Glycoprotein, SulfationInteractioni
Subunit structurei
Homodimer.
Interacts with SLC6A19 (By similarity).
By similarity(Microbial infection) Interacts with FCoV, CCoV, TGEV and HCoV-229E spike glycoprotein.
2 PublicationsProtein-protein interaction databases
STRINGi | 9685.ENSFCAP00000008319 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 33 – 66 | Cytosolic Ser/Thr-rich junctionAdd BLAST | 34 | |
Regioni | 41 – 61 | DisorderedSequence analysisAdd BLAST | 21 | |
Regioni | 67 – 967 | MetalloproteaseAdd BLAST | 901 | |
Regioni | 351 – 355 | Substrate bindingBy similarity | 5 | |
Regioni | 670 – 840 | Interaction with FCoV and TGEV spike glycoprotein2 PublicationsAdd BLAST | 171 |
Sequence similaritiesi
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1046, Eukaryota |
InParanoidi | P79171 |
OrthoDBi | 110058at2759 |
Family and domain databases
CDDi | cd09601, M1_APN-Q_like, 1 hit |
Gene3Di | 1.10.390.10, 1 hit 2.60.40.1730, 1 hit |
InterProi | View protein in InterPro IPR045357, Aminopeptidase_N-like_N IPR042097, Aminopeptidase_N-like_N_sf IPR024571, ERAP1-like_C_dom IPR034016, M1_APN-typ IPR001930, Peptidase_M1 IPR014782, Peptidase_M1_dom IPR027268, Peptidase_M4/M1_CTD_sf |
Pfami | View protein in Pfam PF11838, ERAP1_C, 1 hit PF01433, Peptidase_M1, 1 hit PF17900, Peptidase_M1_N, 1 hit |
PRINTSi | PR00756, ALADIPTASE |
SUPFAMi | SSF63737, SSF63737, 1 hit |
PROSITEi | View protein in PROSITE PS00142, ZINC_PROTEASE, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MAKGFYISKP VGILAILLGV AAVCTIIALS VVYSQEKNRS TESSTAASTA
60 70 80 90 100
APTGPTTTVA TTLDQSKPWN VYRLPKTLIP DSYNVTLRPY LTPNNKGLYV
110 120 130 140 150
FTGTNIVRFT CKESTNIVII HSKRLNYTSH QGHMVALSGV GGFHPQPVIV
160 170 180 190 200
RTELVELTEY LVVHLQEPLV AGRQYEMNSE FQGELADDLA GFYRSEYMEN
210 220 230 240 250
GVKKVLATTH MQATEARKSF PCFDEPAMKA TFNITIIHPN NLVALSNMLP
260 270 280 290 300
RGPSVPFGED PTWKVTEFET TPIMSTYLLA YIVSEFSYVE TRAPSGVLIR
310 320 330 340 350
IWARPSAINQ GHGDYALKVT GPILDFFSQH YDTPYPLNKS DQIALPDFNA
360 370 380 390 400
GAMENWGLVT YRESALLYDR QSSSSGNQER VVTVIAHELA HQWFGNLVTL
410 420 430 440 450
EWWNDLWLNE GFASYVEYLG ADFAEPTWNL KDLMVLNDVY RVMAVDALAS
460 470 480 490 500
SHPLSTPASE INTPAQISEV FDSISYSKGA SVLRMLSNFL TEDLFKMGIA
510 520 530 540 550
SYLHTYKYGN TIYLNLWEHL QQVVDKQPTI KLPDTVSAIM DRWILQMGFP
560 570 580 590 600
VITVDTQTGT ISQQHFLLDP QSVVTRPSQF NYLWIVPISS VRSGSPQAHY
610 620 630 640 650
WLPGVEKAQN DLFKTTANDW VLLNLNVTGY YLVNYDNENW KKIQTQLQTD
660 670 680 690 700
LSVIPVINRA QVIHDAFNLA SAQKVPVTLA LNNTLFLIQE TEYMPWQAAL
710 720 730 740 750
SSLSYFKLMF DRSEVYGPMK RYLKKQVTPL FNHFERVTKN WTDHPQTLMD
760 770 780 790 800
QYSEINAVST ACSYGVPECE KLAATLFAQW KKNPQNNPIH PNLRSTVYCN
810 820 830 840 850
AIAQGGEEEW NFVWEQFLKA ELVNEADKLR GALACSNQVW ILNRFLSYTL
860 870 880 890 900
DPNLIRKQDV TSTLSSISSN VVGQTLVWDF VQSNWKKLFQ DYGTGSFSFS
910 920 930 940 950
NLIQAVTRRF STEFELQQLE QFKKNNMDTG FGSATRALEQ ALEKTKANLK
960
WVKENKDVVL RWFTENS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 105 | N → S in AAD09272 (PubMed:9782265).Curated | 1 | |
Sequence conflicti | 144 | H → Q in AAD09272 (PubMed:9782265).Curated | 1 | |
Sequence conflicti | 178 | N → K in AAD09272 (PubMed:9782265).Curated | 1 | |
Sequence conflicti | 215 | E → D in AAD09272 (PubMed:9782265).Curated | 1 | |
Sequence conflicti | 236 | I → T in AAD09272 (PubMed:9782265).Curated | 1 | |
Sequence conflicti | 258 | Missing in AAD09272 (PubMed:9782265).Curated | 1 | |
Sequence conflicti | 581 | N → K in AAD09272 (PubMed:9782265).Curated | 1 | |
Sequence conflicti | 593 | S → N in AAD09272 (PubMed:9782265).Curated | 1 | |
Sequence conflicti | 623 | L → V in AAD09272 (PubMed:9782265).Curated | 1 | |
Sequence conflicti | 730 | L → S in AAD09272 (PubMed:9782265).Curated | 1 | |
Sequence conflicti | 735 – 737 | ERV → RKS in AAD09272 (PubMed:9782265).Curated | 3 | |
Sequence conflicti | 747 | T → A in AAD09272 (PubMed:9782265).Curated | 1 | |
Sequence conflicti | 829 | L → F in AAD09272 (PubMed:9782265).Curated | 1 | |
Sequence conflicti | 949 | L → I in AAD09272 (PubMed:9782265).Curated | 1 | |
Sequence conflicti | 967 | S → SK in AAD09272 (PubMed:9782265).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U58920 mRNA Translation: AAC48686.1 U96104 mRNA Translation: AAD09272.1 |
RefSeqi | NP_001009252.2, NM_001009252.2 |
Genome annotation databases
GeneIDi | 493785 |
KEGGi | fca:493785 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U58920 mRNA Translation: AAC48686.1 U96104 mRNA Translation: AAD09272.1 |
RefSeqi | NP_001009252.2, NM_001009252.2 |
3D structure databases
AlphaFoldDBi | P79171 |
SMRi | P79171 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9685.ENSFCAP00000008319 |
Protein family/group databases
MEROPSi | M01.001 |
Genome annotation databases
GeneIDi | 493785 |
KEGGi | fca:493785 |
Organism-specific databases
CTDi | 290 |
Phylogenomic databases
eggNOGi | KOG1046, Eukaryota |
InParanoidi | P79171 |
OrthoDBi | 110058at2759 |
Family and domain databases
CDDi | cd09601, M1_APN-Q_like, 1 hit |
Gene3Di | 1.10.390.10, 1 hit 2.60.40.1730, 1 hit |
InterProi | View protein in InterPro IPR045357, Aminopeptidase_N-like_N IPR042097, Aminopeptidase_N-like_N_sf IPR024571, ERAP1-like_C_dom IPR034016, M1_APN-typ IPR001930, Peptidase_M1 IPR014782, Peptidase_M1_dom IPR027268, Peptidase_M4/M1_CTD_sf |
Pfami | View protein in Pfam PF11838, ERAP1_C, 1 hit PF01433, Peptidase_M1, 1 hit PF17900, Peptidase_M1_N, 1 hit |
PRINTSi | PR00756, ALADIPTASE |
SUPFAMi | SSF63737, SSF63737, 1 hit |
PROSITEi | View protein in PROSITE PS00142, ZINC_PROTEASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AMPN_FELCA | |
Accessioni | P79171Primary (citable) accession number: P79171 Secondary accession number(s): O97783 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 136 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families