UniProtKB - P79143 (AMPN_CANLF)
Protein
Aminopeptidase N
Gene
ANPEP
Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Functioni
Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. May have a role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 and regulating its activity (By similarity).By similarity
(Microbial infection) Probable receptor for canine coronavirus (CCoV).1 Publication
Catalytic activityi
- Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.By similarity EC:3.4.11.2
Cofactori
Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 394 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Active sitei | 395 | Proton acceptorPROSITE-ProRule annotation | 1 | |
Metal bindingi | 398 | Zinc; catalyticPROSITE-ProRule annotation | 1 | |
Metal bindingi | 417 | Zinc; catalyticBy similarity | 1 | |
Sitei | 483 | Transition state stabilizerBy similarity | 1 |
GO - Molecular functioni
- metalloaminopeptidase activity Source: GO_Central
- peptide binding Source: GO_Central
- virus receptor activity Source: UniProtKB-KW
- zinc ion binding Source: GO_Central
GO - Biological processi
- angiogenesis Source: UniProtKB-KW
- cell differentiation Source: UniProtKB-KW
- peptide catabolic process Source: GO_Central
- proteolysis Source: GO_Central
- regulation of blood pressure Source: GO_Central
- signal transduction Source: GO_Central
Keywordsi
Molecular function | Aminopeptidase, Developmental protein, Host cell receptor for virus entry, Hydrolase, Metalloprotease, Protease, Receptor |
Biological process | Angiogenesis, Differentiation, Host-virus interaction |
Ligand | Metal-binding, Zinc |
Protein family/group databases
MEROPSi | M01.001 |
Names & Taxonomyi
Protein namesi | Recommended name: Aminopeptidase NCurated (EC:3.4.11.2By similarity)Short name: AP-N Short name: cAPN Alternative name(s): Alanyl aminopeptidase Aminopeptidase M Short name: AP-M Microsomal aminopeptidase CD_antigen: CD13 |
Gene namesi | Name:ANPEP |
Organismi | Canis lupus familiaris (Dog) (Canis familiaris) |
Taxonomic identifieri | 9615 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › |
Proteomesi |
|
Organism-specific databases
VGNCi | VGNC:53146, ANPEP |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Single-pass type II membrane protein By similarity
Note: Also found as a soluble form.By similarity
Extracellular region or secreted
- extracellular exosome Source: Ensembl
Plasma Membrane
- external side of plasma membrane Source: Ensembl
- plasma membrane Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
- endoplasmic reticulum-Golgi intermediate compartment Source: Ensembl
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 11 | CytoplasmicBy similarityAdd BLAST | 11 | |
Transmembranei | 12 – 32 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 33 – 975 | ExtracellularBy similarityAdd BLAST | 943 |
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000095079 | 1 – 975 | Aminopeptidase NAdd BLAST | 975 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 134 | N-linked (GlcNAc...) asparagineBy similarity | 1 | |
Modified residuei | 182 | SulfotyrosineSequence analysis | 1 | |
Glycosylationi | 240 | N-linked (GlcNAc...) asparagineBy similarity | 1 | |
Glycosylationi | 271 | N-linked (GlcNAc...) asparagineBy similarity | 1 | |
Modified residuei | 425 | SulfotyrosineSequence analysis | 1 | |
Modified residuei | 430 | SulfotyrosineSequence analysis | 1 | |
Glycosylationi | 533 | N-linked (GlcNAc...) asparagineBy similarity | 1 | |
Glycosylationi | 580 | N-linked (GlcNAc...) asparagineBy similarity | 1 | |
Glycosylationi | 633 | N-linked (GlcNAc...) asparagineBy similarity | 1 | |
Glycosylationi | 689 | N-linked (GlcNAc...) asparagineBy similarity | 1 | |
Glycosylationi | 747 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 769 ↔ 776 | By similarity | ||
Disulfide bondi | 806 ↔ 842 | By similarity | ||
Glycosylationi | 826 | N-linked (GlcNAc...) asparagineBy similarity | 1 |
Post-translational modificationi
Sulfated.By similarity
N- and O-glycosylated.By similarity
May undergo proteolysis and give rise to a soluble form.By similarity
Keywords - PTMi
Disulfide bond, Glycoprotein, SulfationProteomic databases
PaxDbi | P79143 |
Expressioni
Gene expression databases
Bgeei | ENSCAFG00000012013, Expressed in jejunum and 51 other tissues |
Interactioni
Subunit structurei
(Microbial infection) Interacts with CCoV spike glycoprotein.
1 PublicationHomodimer.
Interacts with SLC6A19 (By similarity).
By similarityProtein-protein interaction databases
STRINGi | 9612.ENSCAFP00000017681 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 33 – 74 | Cytosolic Ser/Thr-rich junctionBy similarityAdd BLAST | 42 | |
Regioni | 75 – 975 | MetalloproteaseAdd BLAST | 901 | |
Regioni | 358 – 362 | Substrate bindingBy similarity | 5 |
Domaini
Amino acids 1-191 are essential to mediate susceptibility to infection with CCV (in canine/human chimeric studies).1 Publication
Sequence similaritiesi
Belongs to the peptidase M1 family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1046, Eukaryota |
GeneTreei | ENSGT00940000154876 |
InParanoidi | P79143 |
OMAi | TAKWFIF |
OrthoDBi | 110058at2759 |
TreeFami | TF300395 |
Family and domain databases
CDDi | cd09601, M1_APN-Q_like, 1 hit |
Gene3Di | 2.60.40.1730, 1 hit |
InterProi | View protein in InterPro IPR042097, Aminopeptidase_N-like_N IPR024571, ERAP1-like_C_dom IPR034016, M1_APN-typ IPR001930, Peptidase_M1 IPR014782, Peptidase_M1_dom |
Pfami | View protein in Pfam PF11838, ERAP1_C, 1 hit PF01433, Peptidase_M1, 1 hit |
PRINTSi | PR00756, ALADIPTASE |
SUPFAMi | SSF63737, SSF63737, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
P79143-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAKGFYISKA LGILAIVLGI AAVSTIIALS VVYAQEKNKN AESSPVSSPV
60 70 80 90 100
SSPVSSPVSP TNPSTTAATT LAQSKPWNHY RLPKTLIPSS YNVTLRPYLT
110 120 130 140 150
PNSNGLYTFK GSSTVRFTCK ESTSMIIIHS KKLNYTNIQG QRVALRGVGG
160 170 180 190 200
SQAPAIDRTE LVEVTEYLVV HLREPLQVNS QYEMDSKFEG ELADDLAGFY
210 220 230 240 250
RSEYTENGVK KVLATTQMQA ADARKSFPCF DEPAMKATFN ITLIHPSNLV
260 270 280 290 300
ALSNMLPRGP SVPFTEEPNW NVTEFETTPI MSTYLLAYIV SEFKNVQENT
310 320 330 340 350
PSNVLIRIWA RPSAMDQGHG NYALRVTGPI LDFFSRHYDT PYPLNKSDQI
360 370 380 390 400
ALPDFNAGAM ENWGLVTYRE SALLYDPQSS SIGNKERVVT VIAHELAHQW
410 420 430 440 450
FGNLVTLEWW NDLWLNEGFA SYVEYLGADY AEPTWNLKDL IVLNEVYRVM
460 470 480 490 500
AVDALASSHP LSSPASEVNT PAQISEVFDS ISYSKGASVL RMLSSFLTED
510 520 530 540 550
LFKKGVASYL HTFAYQNTIY LDLWNHLQWA LGNQTAINLP YTVNAIMDRW
560 570 580 590 600
ILQMGFPVVT VDTTTGTLSQ KHFLLDPQSN VTRPSKFNYL WIIPISSVKS
610 620 630 640 650
GTQQAHYWMP DNAKVQNDLF KTTGDEWVLL NLNVTGYYLV NYDQNNWKKI
660 670 680 690 700
HTQLQTDLSV IPVINRAQVI HDTFDLASAQ IVPVTLALNS TLFLNQETEY
710 720 730 740 750
MPWEAALSSL SYFKLMFDRS EVYGPMKNYL RKQVTPLFNH FEKITQNWTD
760 770 780 790 800
HPQTLTEQYN EINAVSTACT YGVPKCKDLV STLFAEWRKN PQNNPIYPNL
810 820 830 840 850
RSTVYCNAIA QGGEEEWNFV WEQFRNTSLV NEADKLRSAL ACSTQVWILN
860 870 880 890 900
RYLSYTLNPE FIRKQDVIST LSSIASNVIG QSLAWDFIQS NWKKLFEDYG
910 920 930 940 950
TGSFSFSNLI QAVTRRFSTE FELQQLEQFK ANNMDTGFGS GTRALEQALE
960 970
KTKANIKWVK ENKEAVLQWF RENSQ
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A5F4CHX5 | A0A5F4CHX5_CANLF | Aminopeptidase | ANPEP | 938 | Annotation score: | ||
A0A5F4CIA0 | A0A5F4CIA0_CANLF | Aminopeptidase | ANPEP | 966 | Annotation score: | ||
A0A5F4CJ36 | A0A5F4CJ36_CANLF | Aminopeptidase | ANPEP | 919 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAEX03002325 Genomic DNA No translation available. X98239 mRNA Translation: CAA66895.1 |
RefSeqi | NP_001139506.1, NM_001146034.1 |
Genome annotation databases
Ensembli | ENSCAFT00000019074; ENSCAFP00000017681; ENSCAFG00000012013 |
GeneIDi | 403913 |
KEGGi | cfa:403913 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AAEX03002325 Genomic DNA No translation available. X98239 mRNA Translation: CAA66895.1 |
RefSeqi | NP_001139506.1, NM_001146034.1 |
3D structure databases
SMRi | P79143 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 9612.ENSCAFP00000017681 |
Protein family/group databases
MEROPSi | M01.001 |
Proteomic databases
PaxDbi | P79143 |
Genome annotation databases
Ensembli | ENSCAFT00000019074; ENSCAFP00000017681; ENSCAFG00000012013 |
GeneIDi | 403913 |
KEGGi | cfa:403913 |
Organism-specific databases
CTDi | 290 |
VGNCi | VGNC:53146, ANPEP |
Phylogenomic databases
eggNOGi | KOG1046, Eukaryota |
GeneTreei | ENSGT00940000154876 |
InParanoidi | P79143 |
OMAi | TAKWFIF |
OrthoDBi | 110058at2759 |
TreeFami | TF300395 |
Gene expression databases
Bgeei | ENSCAFG00000012013, Expressed in jejunum and 51 other tissues |
Family and domain databases
CDDi | cd09601, M1_APN-Q_like, 1 hit |
Gene3Di | 2.60.40.1730, 1 hit |
InterProi | View protein in InterPro IPR042097, Aminopeptidase_N-like_N IPR024571, ERAP1-like_C_dom IPR034016, M1_APN-typ IPR001930, Peptidase_M1 IPR014782, Peptidase_M1_dom |
Pfami | View protein in Pfam PF11838, ERAP1_C, 1 hit PF01433, Peptidase_M1, 1 hit |
PRINTSi | PR00756, ALADIPTASE |
SUPFAMi | SSF63737, SSF63737, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AMPN_CANLF | |
Accessioni | P79143Primary (citable) accession number: P79143 Secondary accession number(s): F1PCB5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 23, 2003 |
Last sequence update: | March 15, 2017 | |
Last modified: | December 2, 2020 | |
This is version 121 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries