UniProtKB - P78426 (NKX61_HUMAN)
Protein
Homeobox protein Nkx-6.1
Gene
NKX6-1
Organism
Homo sapiens (Human)
Status
Functioni
Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Involved in the development of insulin-producing beta cells in the islets of Langerhans at the secondary transition (By similarity). Together with NKX2-2 and IRX3 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals (By similarity).By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 236 – 295 | HomeoboxPROSITE-ProRule annotationAdd BLAST | 60 |
GO - Molecular functioni
- chromatin binding Source: Ensembl
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: NTNU_SB
- DNA-binding transcription repressor activity, RNA polymerase II-specific Source: Ensembl
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
GO - Biological processi
- animal organ morphogenesis Source: ProtInc
- cell differentiation Source: GO_Central
- cellular response to cytokine stimulus Source: Ensembl
- cellular response to peptide hormone stimulus Source: Ensembl
- detection of glucose Source: BHF-UCL
- negative regulation of glial cell differentiation Source: Ensembl
- negative regulation of transcription by RNA polymerase II Source: BHF-UCL
- neuron migration Source: InterPro
- oligodendrocyte differentiation Source: Ensembl
- pancreas development Source: BHF-UCL
- positive regulation of DNA-binding transcription factor activity Source: BHF-UCL
- positive regulation of glial cell differentiation Source: Ensembl
- positive regulation of insulin secretion Source: Ensembl
- positive regulation of neuron differentiation Source: Ensembl
- positive regulation of transcription by RNA polymerase II Source: BHF-UCL
- positive regulation of type B pancreatic cell development Source: BHF-UCL
- regulation of axon extension Source: Ensembl
- regulation of neuron migration Source: Ensembl
- regulation of transcription by RNA polymerase II Source: GO_Central
- regulation of transcription from RNA polymerase II promoter involved in spinal cord motor neuron fate specification Source: Ensembl
- regulation of transcription from RNA polymerase II promoter involved in ventral spinal cord interneuron specification Source: Ensembl
- response to drug Source: Ensembl
- response to nicotine Source: Ensembl
- smoothened signaling pathway Source: Ensembl
- transcription by RNA polymerase II Source: Ensembl
- type B pancreatic cell maturation Source: BHF-UCL
- type B pancreatic cell proliferation Source: UniProtKB
Keywordsi
Molecular function | Activator, Developmental protein, DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | P78426 |
Reactomei | R-HSA-210745, Regulation of gene expression in beta cells R-HSA-210747, Regulation of gene expression in early pancreatic precursor cells |
SIGNORi | P78426 |
Names & Taxonomyi
Protein namesi | Recommended name: Homeobox protein Nkx-6.1Alternative name(s): Homeobox protein NK-6 homolog A |
Gene namesi | Name:NKX6-1 Synonyms:NKX6A |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000163623.9 |
HGNCi | HGNC:7839, NKX6-1 |
MIMi | 602563, gene |
neXtProti | NX_P78426 |
Subcellular locationi
Nucleus
- Nucleus Curated
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Nucleus
- nuclear chromatin Source: NTNU_SB
- nucleus Source: GO_Central
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
DisGeNETi | 4825 |
OpenTargetsi | ENSG00000163623 |
PharmGKBi | PA31646 |
Miscellaneous databases
Pharosi | P78426, Tbio |
Polymorphism and mutation databases
BioMutai | NKX6-1 |
DMDMi | 288558819 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000048951 | 1 – 367 | Homeobox protein Nkx-6.1Add BLAST | 367 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 189 | Asymmetric dimethylarginineCombined sources | 1 |
Keywords - PTMi
MethylationProteomic databases
EPDi | P78426 |
jPOSTi | P78426 |
MassIVEi | P78426 |
PaxDbi | P78426 |
PeptideAtlasi | P78426 |
PRIDEi | P78426 |
ProteomicsDBi | 57625 |
PTM databases
iPTMneti | P78426 |
PhosphoSitePlusi | P78426 |
Expressioni
Tissue specificityi
Pancreatic beta cells.
Gene expression databases
Bgeei | ENSG00000163623, Expressed in lower esophagus and 69 other tissues |
ExpressionAtlasi | P78426, baseline and differential |
Genevisiblei | P78426, HS |
Organism-specific databases
HPAi | ENSG00000163623, Tissue enhanced (breast, esophagus, pancreas) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 110889, 2 interactors |
IntActi | P78426, 1 interactor |
MINTi | P78426 |
STRINGi | 9606.ENSP00000295886 |
Miscellaneous databases
RNActi | P78426, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 102 – 268 | Repressor domainBy similarityAdd BLAST | 167 | |
Regioni | 306 – 367 | Involved in DNA-bindingBy similarityAdd BLAST | 62 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 49 – 61 | Poly-SerAdd BLAST | 13 | |
Compositional biasi | 119 – 135 | Poly-SerAdd BLAST | 17 | |
Compositional biasi | 136 – 150 | Poly-AlaAdd BLAST | 15 | |
Compositional biasi | 168 – 173 | Poly-Pro | 6 | |
Compositional biasi | 351 – 355 | Poly-Gly | 5 |
Domaini
The C-terminal domain contributes to sequence-specific DNA-binding.By similarity
Keywords - Domaini
HomeoboxPhylogenomic databases
eggNOGi | KOG0847, Eukaryota |
GeneTreei | ENSGT00940000160897 |
HOGENOMi | CLU_064820_0_0_1 |
InParanoidi | P78426 |
OMAi | VGAMEGP |
OrthoDBi | 1263401at2759 |
PhylomeDBi | P78426 |
TreeFami | TF327063 |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR020479, Homeobox_metazoa IPR000047, HTH_motif IPR033630, NKX-6.1 |
PANTHERi | PTHR24340:SF31, PTHR24340:SF31, 1 hit |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit |
PRINTSi | PR00024, HOMEOBOX PR00031, HTHREPRESSR |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
P78426-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLAVGAMEGT RQSAFLLSSP PLAALHSMAE MKTPLYPAAY PPLPAGPPSS
60 70 80 90 100
SSSSSSSSSP SPPLGTHNPG GLKPPATGGL SSLGSPPQQL SAATPHGIND
110 120 130 140 150
ILSRPSMPVA SGAALPSASP SGSSSSSSSS ASASSASAAA AAAAAAAAAA
160 170 180 190 200
SSPAGLLAGL PRFSSLSPPP PPPGLYFSPS AAAVAAVGRY PKPLAELPGR
210 220 230 240 250
TPIFWPGVMQ SPPWRDARLA CTPHQGSILL DKDGKRKHTR PTFSGQQIFA
260 270 280 290 300
LEKTFEQTKY LAGPERARLA YSLGMTESQV KVWFQNRRTK WRKKHAAEMA
310 320 330 340 350
TAKKKQDSET ERLKGASENE EEDDDYNKPL DPNSDDEKIT QLLKKHKSSS
360
GGGGGLLLHA SEPESSS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF8VWZ9 | F8VWZ9_HUMAN | Homeobox protein Nkx-6.1 | NKX6-1 | 93 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 100 | D → N in AAD11962 (PubMed:9119408).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U66799, U66797, U66798 Genomic DNA Translation: AAD11962.1 AC096766 Genomic DNA No translation available. |
CCDSi | CCDS3607.1 |
RefSeqi | NP_006159.2, NM_006168.2 |
Genome annotation databases
Ensembli | ENST00000295886; ENSP00000295886; ENSG00000163623 |
GeneIDi | 4825 |
KEGGi | hsa:4825 |
UCSCi | uc003hpa.2, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U66799, U66797, U66798 Genomic DNA Translation: AAD11962.1 AC096766 Genomic DNA No translation available. |
CCDSi | CCDS3607.1 |
RefSeqi | NP_006159.2, NM_006168.2 |
3D structure databases
SMRi | P78426 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 110889, 2 interactors |
IntActi | P78426, 1 interactor |
MINTi | P78426 |
STRINGi | 9606.ENSP00000295886 |
PTM databases
iPTMneti | P78426 |
PhosphoSitePlusi | P78426 |
Polymorphism and mutation databases
BioMutai | NKX6-1 |
DMDMi | 288558819 |
Proteomic databases
EPDi | P78426 |
jPOSTi | P78426 |
MassIVEi | P78426 |
PaxDbi | P78426 |
PeptideAtlasi | P78426 |
PRIDEi | P78426 |
ProteomicsDBi | 57625 |
Protocols and materials databases
Antibodypediai | 25238, 308 antibodies |
DNASUi | 4825 |
Genome annotation databases
Ensembli | ENST00000295886; ENSP00000295886; ENSG00000163623 |
GeneIDi | 4825 |
KEGGi | hsa:4825 |
UCSCi | uc003hpa.2, human |
Organism-specific databases
CTDi | 4825 |
DisGeNETi | 4825 |
EuPathDBi | HostDB:ENSG00000163623.9 |
GeneCardsi | NKX6-1 |
HGNCi | HGNC:7839, NKX6-1 |
HPAi | ENSG00000163623, Tissue enhanced (breast, esophagus, pancreas) |
MIMi | 602563, gene |
neXtProti | NX_P78426 |
OpenTargetsi | ENSG00000163623 |
PharmGKBi | PA31646 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0847, Eukaryota |
GeneTreei | ENSGT00940000160897 |
HOGENOMi | CLU_064820_0_0_1 |
InParanoidi | P78426 |
OMAi | VGAMEGP |
OrthoDBi | 1263401at2759 |
PhylomeDBi | P78426 |
TreeFami | TF327063 |
Enzyme and pathway databases
PathwayCommonsi | P78426 |
Reactomei | R-HSA-210745, Regulation of gene expression in beta cells R-HSA-210747, Regulation of gene expression in early pancreatic precursor cells |
SIGNORi | P78426 |
Miscellaneous databases
BioGRID-ORCSi | 4825, 11 hits in 866 CRISPR screens |
GeneWikii | NKX6-1 |
GenomeRNAii | 4825 |
Pharosi | P78426, Tbio |
PROi | PR:P78426 |
RNActi | P78426, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000163623, Expressed in lower esophagus and 69 other tissues |
ExpressionAtlasi | P78426, baseline and differential |
Genevisiblei | P78426, HS |
Family and domain databases
CDDi | cd00086, homeodomain, 1 hit |
InterProi | View protein in InterPro IPR009057, Homeobox-like_sf IPR017970, Homeobox_CS IPR001356, Homeobox_dom IPR020479, Homeobox_metazoa IPR000047, HTH_motif IPR033630, NKX-6.1 |
PANTHERi | PTHR24340:SF31, PTHR24340:SF31, 1 hit |
Pfami | View protein in Pfam PF00046, Homeodomain, 1 hit |
PRINTSi | PR00024, HOMEOBOX PR00031, HTHREPRESSR |
SMARTi | View protein in SMART SM00389, HOX, 1 hit |
SUPFAMi | SSF46689, SSF46689, 1 hit |
PROSITEi | View protein in PROSITE PS00027, HOMEOBOX_1, 1 hit PS50071, HOMEOBOX_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NKX61_HUMAN | |
Accessioni | P78426Primary (citable) accession number: P78426 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | February 9, 2010 | |
Last modified: | December 2, 2020 | |
This is version 171 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot