NKX6-1

Functionⁱ

Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Involved in the development of insulin-producing beta cells in the islets of Langerhans at the secondary transition (By similarity). Together with NKX2-2 and IRX3 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals (By similarity).By similarity

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
DNA bindingⁱ	236 – 295	HomeoboxPROSITE-ProRule annotationAdd BLAST		60

GO - Molecular functionⁱ

chromatin binding Source: Ensembl
DNA-binding transcription factor activity, RNA polymerase II-specific Source: NTNU_SB
proximal promoter DNA-binding transcription repressor activity, RNA polymerase II-specific Source: Ensembl
RNA polymerase II proximal promoter sequence-specific DNA binding Source: Ensembl

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

animal organ morphogenesis
Source: ProtInc
Traceable author statementⁱ
- 10567713
cellular response to cytokine stimulus Source: Ensembl
cellular response to peptide hormone stimulus Source: Ensembl
detection of glucose Source: BHF-UCL
negative regulation of glial cell differentiation Source: Ensembl
negative regulation of transcription by RNA polymerase II Source: BHF-UCL
neuron migration Source: InterPro
oligodendrocyte differentiation Source: Ensembl
pancreas development Source: BHF-UCL
positive regulation of DNA-binding transcription factor activity Source: BHF-UCL
positive regulation of glial cell differentiation Source: Ensembl
positive regulation of insulin secretion Source: Ensembl
positive regulation of neuron differentiation Source: Ensembl
positive regulation of transcription by RNA polymerase II Source: BHF-UCL
positive regulation of type B pancreatic cell development Source: BHF-UCL
regulation of axon extension Source: Ensembl
regulation of neuron migration Source: Ensembl
regulation of transcription from RNA polymerase II promoter involved in spinal cord motor neuron fate specification Source: Ensembl
regulation of transcription from RNA polymerase II promoter involved in ventral spinal cord interneuron specification Source: Ensembl
response to nicotine Source: Ensembl
smoothened signaling pathway Source: Ensembl
transcription by RNA polymerase II Source: Ensembl
type B pancreatic cell maturation Source: BHF-UCL
type B pancreatic cell proliferation Source: UniProtKB

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Activator, Developmental protein, DNA-binding, Repressor
Biological process	Transcription, Transcription regulation

Enzyme and pathway databases

Reactomeⁱ	R-HSA-210745 Regulation of gene expression in beta cells R-HSA-210747 Regulation of gene expression in early pancreatic precursor cells
SIGNORⁱ	P78426

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Homeobox protein Nkx-6.1 Alternative name(s): Homeobox protein NK-6 homolog A
Gene namesⁱ	Name:NKX6-1 Synonyms:NKX6A
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 4

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000163623.9
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:7839 NKX6-1
MIMⁱ	602563 gene
neXtProtⁱ	NX_P78426

Keywords - Cellular componentⁱ

Nucleus

Pathology & Biotechⁱ

Organism-specific databases

DisGeNET More...DisGeNETⁱ	4825
Open Targets More...OpenTargetsⁱ	ENSG00000163623
PharmGKBⁱ	PA31646

Polymorphism and mutation databases

BioMutaⁱ	NKX6-1
DMDMⁱ	288558819

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000048951	1 – 367	Homeobox protein Nkx-6.1Add BLAST		367

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	189	Asymmetric dimethylarginineCombined sources		1

Keywords - PTMⁱ

Methylation

Proteomic databases

EPDⁱ	P78426
PaxDbⁱ	P78426
PeptideAtlas More...PeptideAtlasⁱ	P78426
PRIDEⁱ	P78426
ProteomicsDBⁱ	57625

PTM databases

iPTMnetⁱ	P78426
PhosphoSitePlusⁱ	P78426

Expressionⁱ

Tissue specificityⁱ

Pancreatic beta cells.

Gene expression databases

Bgeeⁱ	ENSG00000163623 Expressed in 49 organ(s), highest expression level in lower esophagus
CleanExⁱ	HS_NKX6-1
ExpressionAtlasⁱ	P78426 baseline and differential
Genevisibleⁱ	P78426 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	CAB070169 HPA036774

HPAⁱ

CAB070169
HPA036774

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	110889, 2 interactors
IntActⁱ	P78426, 1 interactor
Molecular INTeraction database More...MINTⁱ	P78426
STRINGⁱ	9606.ENSP00000295886

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P78426
SMRⁱ	P78426
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	102 – 268	Repressor domainBy similarityAdd BLAST		167
Regionⁱ	306 – 367	Involved in DNA-bindingBy similarityAdd BLAST		62

Compositional bias

Feature key	Position(s)	DescriptionActions	Length
Compositional biasⁱ	49 – 61	Poly-SerAdd BLAST	13
Compositional biasⁱ	119 – 135	Poly-SerAdd BLAST	17
Compositional biasⁱ	136 – 150	Poly-AlaAdd BLAST	15
Compositional biasⁱ	168 – 173	Poly-Pro	6
Compositional biasⁱ	351 – 355	Poly-Gly	5

Domainⁱ

The C-terminal domain contributes to sequence-specific DNA-binding.By similarity

Keywords - Domainⁱ

Homeobox

Phylogenomic databases

eggNOGⁱ	KOG0847 Eukaryota ENOG410XT1X LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00930000150864
HOGENOMⁱ	HOG000060127
HOVERGENⁱ	HBG070987
InParanoidⁱ	P78426
KEGG Orthology (KO) More...KOⁱ	K08030
OMAⁱ	VGAMEGP
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G142D
PhylomeDBⁱ	P78426
TreeFamⁱ	TF327063

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00086 homeodomain, 1 hit
InterProⁱ	View protein in InterPro IPR009057 Homeobox-like_sf IPR017970 Homeobox_CS IPR001356 Homeobox_dom IPR020479 Homeobox_metazoa IPR000047 HTH_motif IPR033630 NKX-6.1
PANTHERⁱ	PTHR24340:SF31 PTHR24340:SF31, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00046 Homeobox, 1 hit
PRINTSⁱ	PR00024 HOMEOBOX PR00031 HTHREPRESSR
SMARTⁱ	View protein in SMART SM00389 HOX, 1 hit
SUPFAMⁱ	SSF46689 SSF46689, 1 hit
PROSITEⁱ	View protein in PROSITE PS00027 HOMEOBOX_1, 1 hit PS50071 HOMEOBOX_2, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

P78426-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MLAVGAMEGT RQSAFLLSSP PLAALHSMAE MKTPLYPAAY PPLPAGPPSS 
        60         70         80         90        100
SSSSSSSSSP SPPLGTHNPG GLKPPATGGL SSLGSPPQQL SAATPHGIND 
       110        120        130        140        150
ILSRPSMPVA SGAALPSASP SGSSSSSSSS ASASSASAAA AAAAAAAAAA 
       160        170        180        190        200
SSPAGLLAGL PRFSSLSPPP PPPGLYFSPS AAAVAAVGRY PKPLAELPGR 
       210        220        230        240        250
TPIFWPGVMQ SPPWRDARLA CTPHQGSILL DKDGKRKHTR PTFSGQQIFA 
       260        270        280        290        300
LEKTFEQTKY LAGPERARLA YSLGMTESQV KVWFQNRRTK WRKKHAAEMA 
       310        320        330        340        350
TAKKKQDSET ERLKGASENE EEDDDYNKPL DPNSDDEKIT QLLKKHKSSS 
       360 
GGGGGLLLHA SEPESSS

Length:367

Mass (Da):37,849

Last modified:February 9, 2010 - v2

Checksum:ⁱ5C78F06C3CAE6486

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	F8VWZ9	F8VWZ9_HUMAN		Homeobox protein Nkx-6.1 Homeobox protein Nkx-6.1	NKX6-1	93	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	100	D → N in AAD11962 (PubMed:9119408).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U66799, U66797, U66798 Genomic DNA Translation: AAD11962.1 AC096766 Genomic DNA No translation available.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS3607.1
NCBI Reference Sequences More...RefSeqⁱ	NP_006159.2, NM_006168.2
UniGeneⁱ	Hs.546270

Genome annotation databases

Ensemblⁱ	ENST00000295886; ENSP00000295886; ENSG00000163623
GeneIDⁱ	4825
KEGGⁱ	hsa:4825
UCSC genome browser More...UCSCⁱ	uc003hpa.2 human

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P78426	NK6 homeobox 1		PANTR		367	UniRef100_P78426
Homeobox protein Nkx-6.1		HUMAN		93
Uncharacterized protein		PANPA		93
NKX6-1 isoform 1		PANTR		93

Protein	Similar proteins		Species	Score	Length	Source
P78426	Homeobox protein Nkx-6.1		MOUSE		365	UniRef90_Q99MA9
Homeobox protein Nkx-6.1		RAT		365
Homeobox protein Nkx-6.1		MESAU		364
Uncharacterized protein		PIG		370
Uncharacterized protein		RABIT		369
+76

Protein	Similar proteins		Species	Score	Length	Source
P78426	Homeobox protein Nkx-6.1		MOUSE		365	UniRef50_Q99MA9
Homeobox protein Nkx-6.1		RAT		365
Homeobox protein Nkx-6.1		MESAU		364
Uncharacterized protein		PIG		370
Uncharacterized protein		RABIT		369
+79

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U66799, U66797, U66798 Genomic DNA Translation: AAD11962.1 AC096766 Genomic DNA No translation available.
CCDSⁱ	CCDS3607.1
RefSeqⁱ	NP_006159.2, NM_006168.2
UniGeneⁱ	Hs.546270

3D structure databases

ProteinModelPortalⁱ	P78426
SMRⁱ	P78426
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	110889, 2 interactors
IntActⁱ	P78426, 1 interactor
MINTⁱ	P78426
STRINGⁱ	9606.ENSP00000295886

PTM databases

iPTMnetⁱ	P78426
PhosphoSitePlusⁱ	P78426

Polymorphism and mutation databases

BioMutaⁱ	NKX6-1
DMDMⁱ	288558819

Proteomic databases

EPDⁱ	P78426
PaxDbⁱ	P78426
PeptideAtlasⁱ	P78426
PRIDEⁱ	P78426
ProteomicsDBⁱ	57625

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	4825
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000295886; ENSP00000295886; ENSG00000163623
GeneIDⁱ	4825
KEGGⁱ	hsa:4825
UCSCⁱ	uc003hpa.2 human

Organism-specific databases

CTDⁱ	4825
DisGeNETⁱ	4825
EuPathDBⁱ	HostDB:ENSG00000163623.9
GeneCardsⁱ	NKX6-1
HGNCⁱ	HGNC:7839 NKX6-1
HPAⁱ	CAB070169 HPA036774
MIMⁱ	602563 gene
neXtProtⁱ	NX_P78426
OpenTargetsⁱ	ENSG00000163623
PharmGKBⁱ	PA31646
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0847 Eukaryota ENOG410XT1X LUCA
GeneTreeⁱ	ENSGT00930000150864
HOGENOMⁱ	HOG000060127
HOVERGENⁱ	HBG070987
InParanoidⁱ	P78426
KOⁱ	K08030
OMAⁱ	VGAMEGP
OrthoDBⁱ	EOG091G142D
PhylomeDBⁱ	P78426
TreeFamⁱ	TF327063

Enzyme and pathway databases

Reactomeⁱ	R-HSA-210745 Regulation of gene expression in beta cells R-HSA-210747 Regulation of gene expression in early pancreatic precursor cells
SIGNORⁱ	P78426

Miscellaneous databases

ChiTaRSⁱ	NKX6-1 human
GeneWikiⁱ	NKX6-1
GenomeRNAiⁱ	4825
Protein Ontology More...PROⁱ	PR:P78426
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000163623 Expressed in 49 organ(s), highest expression level in lower esophagus
CleanExⁱ	HS_NKX6-1
ExpressionAtlasⁱ	P78426 baseline and differential
Genevisibleⁱ	P78426 HS

Family and domain databases

CDDⁱ	cd00086 homeodomain, 1 hit
InterProⁱ	View protein in InterPro IPR009057 Homeobox-like_sf IPR017970 Homeobox_CS IPR001356 Homeobox_dom IPR020479 Homeobox_metazoa IPR000047 HTH_motif IPR033630 NKX-6.1
PANTHERⁱ	PTHR24340:SF31 PTHR24340:SF31, 1 hit
Pfamⁱ	View protein in Pfam PF00046 Homeobox, 1 hit
PRINTSⁱ	PR00024 HOMEOBOX PR00031 HTHREPRESSR
SMARTⁱ	View protein in SMART SM00389 HOX, 1 hit
SUPFAMⁱ	SSF46689 SSF46689, 1 hit
PROSITEⁱ	View protein in PROSITE PS00027 HOMEOBOX_1, 1 hit PS50071 HOMEOBOX_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	NKX61_HUMAN
Accessionⁱ	P78426Primary (citable) accession number: P78426
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
	Last sequence update:	February 9, 2010
	Last modified:	November 7, 2018
	This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

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UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P78426 (NKX61_HUMAN)

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Homeobox protein Nkx-6.1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Extracellular region or secreted

Nucleus

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Compositional bias

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ