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Protein

Glutathione S-transferase omega-1

Gene

GSTO1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Exhibits glutathione-dependent thiol transferase and dehydroascorbate reductase activities. Has S-(phenacyl)glutathione reductase activity. Has also glutathione S-transferase activity. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) and dimethylarsonic acid.5 Publications

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.5 Publications
2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate.2 Publications
Methylarsonate + 2 glutathione = methylarsonite + glutathione disulfide + H2O.1 Publication

Activity regulationi

Monomethylarsonic acid reductase activity is competitively inhibited by 1-chloro 2,4-dinitrobenzene (CDNB) and by deoxycholate.

pH dependencei

Optimum pH is 8.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei32Nucleophile1 Publication1
Binding sitei59Glutathione2 Publications1
Binding sitei72Glutathione; via amide nitrogen and carbonyl oxygen2 Publications1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase, Transferase

Enzyme and pathway databases

BioCyciMetaCyc:HS07564-MONOMER
BRENDAi1.20.4.2 2681
1.8.5.1 2681
2.5.1.18 2681
ReactomeiR-HSA-156581 Methylation
R-HSA-156590 Glutathione conjugation
R-HSA-196836 Vitamin C (ascorbate) metabolism
R-HSA-8950505 Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase omega-1 (EC:2.5.1.185 Publications)
Short name:
GSTO-1
Alternative name(s):
Glutathione S-transferase omega 1-1
Short name:
GSTO 1-1
Glutathione-dependent dehydroascorbate reductase (EC:1.8.5.12 Publications)
Monomethylarsonic acid reductase (EC:1.20.4.21 Publication)
Short name:
MMA(V) reductase
S-(Phenacyl)glutathione reductase
Short name:
SPG-R
Gene namesi
Name:GSTO1
Synonyms:GSTTLP28
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiHostDB:ENSG00000148834.12
HGNCiHGNC:13312 GSTO1
MIMi605482 gene
neXtProtiNX_P78417

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi32C → A: Loss of activity. 1 Publication1

Organism-specific databases

DisGeNETi9446
OpenTargetsiENSG00000148834
PharmGKBiPA133787054

Chemistry databases

ChEMBLiCHEMBL3174
DrugBankiDB00143 Glutathione
DB00163 Vitamin E

Polymorphism and mutation databases

BioMutaiGSTO1
DMDMi6016173

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001858842 – 241Glutathione S-transferase omega-1Add BLAST240

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei57N6-acetyllysineCombined sources1
Modified residuei129PhosphoserineCombined sources1
Modified residuei143N6-acetyllysineCombined sources1
Modified residuei148N6-acetyllysineCombined sources1
Modified residuei152N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP78417
PaxDbiP78417
PeptideAtlasiP78417
PRIDEiP78417
ProteomicsDBi57621
TopDownProteomicsiP78417-1 [P78417-1]

2D gel databases

OGPiP78417
UCD-2DPAGEiP78417

PTM databases

iPTMnetiP78417
PhosphoSitePlusiP78417
SwissPalmiP78417

Expressioni

Tissue specificityi

Ubiquitous. Highest expression in liver, pancreas, skeletal muscle, spleen, thymus, colon, blood leukocyte and heart. Lowest expression in brain, placenta and lung.1 Publication

Gene expression databases

BgeeiENSG00000148834 Expressed in 226 organ(s), highest expression level in liver
CleanExiHS_GSTO1
ExpressionAtlasiP78417 baseline and differential
GenevisibleiP78417 HS

Organism-specific databases

HPAiHPA037603
HPA037604

Interactioni

Subunit structurei

Homodimer.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HSPB1P047922EBI-712083,EBI-352682

Protein-protein interaction databases

BioGridi114836, 38 interactors
IntActiP78417, 8 interactors
STRINGi9606.ENSP00000358727

Chemistry databases

BindingDBiP78417

Structurei

Secondary structure

1241
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP78417
SMRiP78417
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP78417

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 101GST N-terminalAdd BLAST80
Domaini106 – 230GST C-terminalAdd BLAST125

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni85 – 86Glutathione binding2 Publications2

Sequence similaritiesi

Belongs to the GST superfamily. Omega family.Curated

Phylogenomic databases

eggNOGiKOG0406 Eukaryota
ENOG410XSIX LUCA
GeneTreeiENSGT00390000005479
HOGENOMiHOG000006560
HOVERGENiHBG051853
InParanoidiP78417
KOiK00799
OMAiEHPLKLY
OrthoDBiEOG091G0IEA
PhylomeDBiP78417
TreeFamiTF105325

Family and domain databases

InterProiView protein in InterPro
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR040079 Glutathione_S-Trfase
IPR004045 Glutathione_S-Trfase_N
IPR004046 GST_C
IPR005442 GST_omega
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF14497 GST_C_3, 1 hit
PF13417 GST_N_3, 1 hit
PRINTSiPR01625 GSTRNSFRASEO
SFLDiSFLDS00019 Glutathione_Transferase_(cytos, 1 hit
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS50405 GST_CTER, 1 hit
PS50404 GST_NTER, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P78417-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSGESARSLG KGSAPPGPVP EGSIRIYSMR FCPFAERTRL VLKAKGIRHE
60 70 80 90 100
VININLKNKP EWFFKKNPFG LVPVLENSQG QLIYESAITC EYLDEAYPGK
110 120 130 140 150
KLLPDDPYEK ACQKMILELF SKVPSLVGSF IRSQNKEDYA GLKEEFRKEF
160 170 180 190 200
TKLEEVLTNK KTTFFGGNSI SMIDYLIWPW FERLEAMKLN ECVDHTPKLK
210 220 230 240
LWMAAMKEDP TVSALLTSEK DWQGFLELYL QNSPEACDYG L
Length:241
Mass (Da):27,566
Last modified:January 1, 1998 - v2
Checksum:i9134ABA265F5C87E
GO
Isoform 2 (identifier: P78417-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     123-155: Missing.

Show »
Length:208
Mass (Da):23,709
Checksum:iF00CDE200E2A1738
GO
Isoform 3 (identifier: P78417-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-28: Missing.

Show »
Length:213
Mass (Da):24,796
Checksum:iC3F8EBA348399628
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5TA02Q5TA02_HUMAN
Glutathione S-transferase omega-1
GSTO1
200Annotation score:
Q5TA01Q5TA01_HUMAN
Glutathione S-transferase omega-1
GSTO1
180Annotation score:

Sequence cautioni

The sequence CAD97673 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_06123132C → Y. Corresponds to variant dbSNP:rs45529437Ensembl.1
Natural variantiVAR_02926986S → C. Corresponds to variant dbSNP:rs11509436Ensembl.1
Natural variantiVAR_016811140A → D in allele GSTO1*C; no effect on protein stability. 3 PublicationsCorresponds to variant dbSNP:rs4925Ensembl.1
Natural variantiVAR_016813155Missing in allele GSTO1*B; decreased protein stability. 3 Publications1
Natural variantiVAR_024484208E → K1 PublicationCorresponds to variant dbSNP:rs11509438Ensembl.1
Natural variantiVAR_026583236A → V1 PublicationCorresponds to variant dbSNP:rs11509439Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0458191 – 28Missing in isoform 3. CuratedAdd BLAST28
Alternative sequenceiVSP_045820123 – 155Missing in isoform 2. CuratedAdd BLAST33

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90313 mRNA Translation: AAB70109.1
AF212303 mRNA Translation: AAF73376.1
AY817669 Genomic DNA Translation: AAV68046.1
BX537431 mRNA Translation: CAD97673.1 Different initiation.
AL139341 Genomic DNA No translation available.
CH471066 Genomic DNA Translation: EAW49601.1
CH471066 Genomic DNA Translation: EAW49602.1
CH471066 Genomic DNA Translation: EAW49603.1
BC000127 mRNA Translation: AAH00127.1
CCDSiCCDS53572.1 [P78417-2]
CCDS53573.1 [P78417-3]
CCDS7555.1 [P78417-1]
RefSeqiNP_001177931.1, NM_001191002.1 [P78417-2]
NP_001177932.1, NM_001191003.1 [P78417-3]
NP_004823.1, NM_004832.2 [P78417-1]
UniGeneiHs.190028

Genome annotation databases

EnsembliENST00000369710; ENSP00000358724; ENSG00000148834 [P78417-2]
ENST00000369713; ENSP00000358727; ENSG00000148834 [P78417-1]
ENST00000539281; ENSP00000441488; ENSG00000148834 [P78417-3]
GeneIDi9446
KEGGihsa:9446
UCSCiuc021pxr.2 human [P78417-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U90313 mRNA Translation: AAB70109.1
AF212303 mRNA Translation: AAF73376.1
AY817669 Genomic DNA Translation: AAV68046.1
BX537431 mRNA Translation: CAD97673.1 Different initiation.
AL139341 Genomic DNA No translation available.
CH471066 Genomic DNA Translation: EAW49601.1
CH471066 Genomic DNA Translation: EAW49602.1
CH471066 Genomic DNA Translation: EAW49603.1
BC000127 mRNA Translation: AAH00127.1
CCDSiCCDS53572.1 [P78417-2]
CCDS53573.1 [P78417-3]
CCDS7555.1 [P78417-1]
RefSeqiNP_001177931.1, NM_001191002.1 [P78417-2]
NP_001177932.1, NM_001191003.1 [P78417-3]
NP_004823.1, NM_004832.2 [P78417-1]
UniGeneiHs.190028

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EEMX-ray2.00A1-241[»]
3LFLX-ray2.10A/B/C1-241[»]
3VLNX-ray1.70A1-241[»]
4IS0X-ray1.72A1-241[»]
4YQMX-ray2.38A/B/C1-241[»]
4YQUX-ray1.94A/B1-241[»]
4YQVX-ray2.06A/B/C1-241[»]
5UEHX-ray2.00A1-241[»]
5V3QX-ray2.25A1-241[»]
ProteinModelPortaliP78417
SMRiP78417
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114836, 38 interactors
IntActiP78417, 8 interactors
STRINGi9606.ENSP00000358727

Chemistry databases

BindingDBiP78417
ChEMBLiCHEMBL3174
DrugBankiDB00143 Glutathione
DB00163 Vitamin E

PTM databases

iPTMnetiP78417
PhosphoSitePlusiP78417
SwissPalmiP78417

Polymorphism and mutation databases

BioMutaiGSTO1
DMDMi6016173

2D gel databases

OGPiP78417
UCD-2DPAGEiP78417

Proteomic databases

EPDiP78417
PaxDbiP78417
PeptideAtlasiP78417
PRIDEiP78417
ProteomicsDBi57621
TopDownProteomicsiP78417-1 [P78417-1]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000369710; ENSP00000358724; ENSG00000148834 [P78417-2]
ENST00000369713; ENSP00000358727; ENSG00000148834 [P78417-1]
ENST00000539281; ENSP00000441488; ENSG00000148834 [P78417-3]
GeneIDi9446
KEGGihsa:9446
UCSCiuc021pxr.2 human [P78417-1]

Organism-specific databases

CTDi9446
DisGeNETi9446
EuPathDBiHostDB:ENSG00000148834.12
GeneCardsiGSTO1
HGNCiHGNC:13312 GSTO1
HPAiHPA037603
HPA037604
MIMi605482 gene
neXtProtiNX_P78417
OpenTargetsiENSG00000148834
PharmGKBiPA133787054
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0406 Eukaryota
ENOG410XSIX LUCA
GeneTreeiENSGT00390000005479
HOGENOMiHOG000006560
HOVERGENiHBG051853
InParanoidiP78417
KOiK00799
OMAiEHPLKLY
OrthoDBiEOG091G0IEA
PhylomeDBiP78417
TreeFamiTF105325

Enzyme and pathway databases

BioCyciMetaCyc:HS07564-MONOMER
BRENDAi1.20.4.2 2681
1.8.5.1 2681
2.5.1.18 2681
ReactomeiR-HSA-156581 Methylation
R-HSA-156590 Glutathione conjugation
R-HSA-196836 Vitamin C (ascorbate) metabolism
R-HSA-8950505 Gene and protein expression by JAK-STAT signaling after Interleukin-12 stimulation

Miscellaneous databases

ChiTaRSiGSTO1 human
EvolutionaryTraceiP78417
GeneWikiiGSTO1
GenomeRNAii9446
PROiPR:P78417
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000148834 Expressed in 226 organ(s), highest expression level in liver
CleanExiHS_GSTO1
ExpressionAtlasiP78417 baseline and differential
GenevisibleiP78417 HS

Family and domain databases

InterProiView protein in InterPro
IPR010987 Glutathione-S-Trfase_C-like
IPR036282 Glutathione-S-Trfase_C_sf
IPR040079 Glutathione_S-Trfase
IPR004045 Glutathione_S-Trfase_N
IPR004046 GST_C
IPR005442 GST_omega
IPR036249 Thioredoxin-like_sf
PfamiView protein in Pfam
PF14497 GST_C_3, 1 hit
PF13417 GST_N_3, 1 hit
PRINTSiPR01625 GSTRNSFRASEO
SFLDiSFLDS00019 Glutathione_Transferase_(cytos, 1 hit
SUPFAMiSSF47616 SSF47616, 1 hit
SSF52833 SSF52833, 1 hit
PROSITEiView protein in PROSITE
PS50405 GST_CTER, 1 hit
PS50404 GST_NTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGSTO1_HUMAN
AccessioniPrimary (citable) accession number: P78417
Secondary accession number(s): D3DRA3
, F5H7H0, Q5TA03, Q7Z3T2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: November 7, 2018
This is version 194 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
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