UniProtKB - P78395 (PRAME_HUMAN)
Protein
Melanoma antigen preferentially expressed in tumors
Gene
PRAME
Organism
Homo sapiens (Human)
Status
Functioni
Functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. Prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis.1 Publication
Miscellaneous
Tumor antigen recognized by cytolytic T lymphocytes.
GO - Molecular functioni
- retinoic acid receptor binding Source: UniProtKB
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- cell differentiation Source: UniProtKB-KW
- negative regulation of apoptotic process Source: UniProtKB
- negative regulation of cell differentiation Source: UniProtKB
- negative regulation of retinoic acid receptor signaling pathway Source: UniProtKB
- negative regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of cell population proliferation Source: UniProtKB
- regulation of growth Source: UniProtKB-KW
Keywordsi
Molecular function | Repressor |
Biological process | Apoptosis, Differentiation, Growth regulation, Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | P78395 |
SIGNORi | P78395 |
Names & Taxonomyi
Protein namesi | Recommended name: Melanoma antigen preferentially expressed in tumorsCuratedAlternative name(s): Opa-interacting protein 4 Short name: OIP-4 Preferentially expressed antigen of melanoma |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:9336, PRAME |
MIMi | 606021, gene |
neXtProti | NX_P78395 |
VEuPathDBi | HostDB:ENSG00000185686.17 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication
Nucleus
- Nucleus 1 Publication
Nucleus
- nucleoplasm Source: HPA
Plasma Membrane
- plasma membrane Source: HPA
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
Cell membrane, Membrane, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 470 – 471 | LL → VV: Loss of interaction with RARA and defective in repressing RARA signaling. 1 Publication | 2 |
Organism-specific databases
DisGeNETi | 23532 |
OpenTargetsi | ENSG00000185686 |
PharmGKBi | PA33698 |
Miscellaneous databases
Pharosi | P78395, Tbio |
Genetic variation databases
BioMutai | PRAME |
DMDMi | 6685631 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000156973 | 1 – 509 | Melanoma antigen preferentially expressed in tumorsAdd BLAST | 509 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 9 | PhosphoserineCombined sources | 1 | |
Modified residuei | 16 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | P78395 |
jPOSTi | P78395 |
MassIVEi | P78395 |
MaxQBi | P78395 |
PaxDbi | P78395 |
PeptideAtlasi | P78395 |
PRIDEi | P78395 |
ProteomicsDBi | 57611 |
PTM databases
iPTMneti | P78395 |
PhosphoSitePlusi | P78395 |
Expressioni
Tissue specificityi
Expressed in testis. Detected in samples of kidney, brain and skin.1 Publication
Gene expression databases
Bgeei | ENSG00000185686, Expressed in right testis and 91 other tissues |
ExpressionAtlasi | P78395, baseline and differential |
Genevisiblei | P78395, HS |
Organism-specific databases
HPAi | ENSG00000185686, Tissue enriched (testis) |
Interactioni
Subunit structurei
Interacts with RARA (via the ligand-binding domain); the interaction is direct and ligand (retinoic acid)-dependent.
Interacts with EZH2; required to repress RAR signaling.
1 PublicationGO - Molecular functioni
- retinoic acid receptor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 117078, 59 interactors |
CORUMi | P78395 |
IntActi | P78395, 32 interactors |
MINTi | P78395 |
STRINGi | 9606.ENSP00000445675 |
Miscellaneous databases
RNActi | P78395, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 116 – 145 | LRR 1; degenerateBy similarityAdd BLAST | 30 | |
Repeati | 207 – 231 | LRR 2; degenerateBy similarityAdd BLAST | 25 | |
Repeati | 232 – 258 | LRR 3; degenerateBy similarityAdd BLAST | 27 | |
Repeati | 259 – 294 | LRR 4; degenerateBy similarityAdd BLAST | 36 | |
Repeati | 295 – 320 | LRR 5By similarityAdd BLAST | 26 | |
Repeati | 321 – 352 | LRR 6By similarityAdd BLAST | 32 | |
Repeati | 353 – 371 | LRR 7By similarityAdd BLAST | 19 | |
Repeati | 377 – 404 | LRR 8By similarityAdd BLAST | 28 | |
Repeati | 405 – 429 | LRR 9By similarityAdd BLAST | 25 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 416 – 509 | Mediates interaction with RARA1 PublicationAdd BLAST | 94 |
Sequence similaritiesi
Belongs to the PRAME family.Curated
Keywords - Domaini
Leucine-rich repeat, RepeatPhylogenomic databases
eggNOGi | ENOG502QWSJ, Eukaryota |
GeneTreei | ENSGT00990000203552 |
HOGENOMi | CLU_039635_2_1_1 |
InParanoidi | P78395 |
OMAi | TFTFCGN |
OrthoDBi | 631249at2759 |
PhylomeDBi | P78395 |
TreeFami | TF332708 |
Family and domain databases
Gene3Di | 3.80.10.10, 1 hit |
InterProi | View protein in InterPro IPR032675, LRR_dom_sf IPR026271, PRAME_family |
PIRSFi | PIRSF038286, PRAME, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 8 potential isoforms that are computationally mapped.Show allAlign All
P78395-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MERRRLWGSI QSRYISMSVW TSPRRLVELA GQSLLKDEAL AIAALELLPR
60 70 80 90 100
ELFPPLFMAA FDGRHSQTLK AMVQAWPFTC LPLGVLMKGQ HLHLETFKAV
110 120 130 140 150
LDGLDVLLAQ EVRPRRWKLQ VLDLRKNSHQ DFWTVWSGNR ASLYSFPEPE
160 170 180 190 200
AAQPMTKKRK VDGLSTEAEQ PFIPVEVLVD LFLKEGACDE LFSYLIEKVK
210 220 230 240 250
RKKNVLRLCC KKLKIFAMPM QDIKMILKMV QLDSIEDLEV TCTWKLPTLA
260 270 280 290 300
KFSPYLGQMI NLRRLLLSHI HASSYISPEK EEQYIAQFTS QFLSLQCLQA
310 320 330 340 350
LYVDSLFFLR GRLDQLLRHV MNPLETLSIT NCRLSEGDVM HLSQSPSVSQ
360 370 380 390 400
LSVLSLSGVM LTDVSPEPLQ ALLERASATL QDLVFDECGI TDDQLLALLP
410 420 430 440 450
SLSHCSQLTT LSFYGNSISI SALQSLLQHL IGLSNLTHVL YPVPLESYED
460 470 480 490 500
IHGTLHLERL AYLHARLREL LCELGRPSMV WLSANPCPHC GDRTFYDPEP
ILCPCFMPN
Computationally mapped potential isoform sequencesi
There are 8 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB5MD04 | B5MD04_HUMAN | Melanoma antigen preferentially-exp... | PRAME | 86 | Annotation score: | ||
E7EMH2 | E7EMH2_HUMAN | Melanoma antigen preferentially-exp... | PRAME | 152 | Annotation score: | ||
H7C2P3 | H7C2P3_HUMAN | Melanoma antigen preferentially-exp... | PRAME | 189 | Annotation score: | ||
B5MCY4 | B5MCY4_HUMAN | Melanoma antigen preferentially-exp... | PRAME | 115 | Annotation score: | ||
E7EW99 | E7EW99_HUMAN | Melanoma antigen preferentially-exp... | PRAME | 201 | Annotation score: | ||
F8WF80 | F8WF80_HUMAN | Melanoma antigen preferentially-exp... | PRAME | 64 | Annotation score: | ||
A0A0G2JRX7 | A0A0G2JRX7_HUMAN | Melanoma antigen preferentially-exp... | PRAME | 149 | Annotation score: | ||
A0A0G2JRX8 | A0A0G2JRX8_HUMAN | Melanoma antigen preferentially-exp... | PRAME | 114 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 452 | H → D in AAC39560 (PubMed:9466265).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_021258 | 7 | W → R2 PublicationsCorresponds to variant dbSNP:rs1129172Ensembl. | 1 | |
Natural variantiVAR_062137 | 218 | M → V. Corresponds to variant dbSNP:rs41277507Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U65011 mRNA Translation: AAC51160.1 CR456549 mRNA Translation: CAG30435.1 AK312769 mRNA Translation: BAG35634.1 CH471095 Genomic DNA Translation: EAW59518.1 BC014074 mRNA Translation: AAH14074.1 BC022008 mRNA Translation: AAH22008.1 BC039731 mRNA Translation: AAH39731.1 AF025440 mRNA Translation: AAC39560.1 |
CCDSi | CCDS13801.1 |
RefSeqi | NP_001278644.1, NM_001291715.1 NP_001278645.1, NM_001291716.1 NP_001278646.1, NM_001291717.1 NP_001278648.1, NM_001291719.1 NP_001305055.1, NM_001318126.1 NP_001305056.1, NM_001318127.1 NP_006106.1, NM_006115.4 NP_996836.1, NM_206953.2 NP_996837.1, NM_206954.2 NP_996838.1, NM_206955.2 NP_996839.1, NM_206956.2 |
Genome annotation databases
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U65011 mRNA Translation: AAC51160.1 CR456549 mRNA Translation: CAG30435.1 AK312769 mRNA Translation: BAG35634.1 CH471095 Genomic DNA Translation: EAW59518.1 BC014074 mRNA Translation: AAH14074.1 BC022008 mRNA Translation: AAH22008.1 BC039731 mRNA Translation: AAH39731.1 AF025440 mRNA Translation: AAC39560.1 |
CCDSi | CCDS13801.1 |
RefSeqi | NP_001278644.1, NM_001291715.1 NP_001278645.1, NM_001291716.1 NP_001278646.1, NM_001291717.1 NP_001278648.1, NM_001291719.1 NP_001305055.1, NM_001318126.1 NP_001305056.1, NM_001318127.1 NP_006106.1, NM_006115.4 NP_996836.1, NM_206953.2 NP_996837.1, NM_206954.2 NP_996838.1, NM_206955.2 NP_996839.1, NM_206956.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 117078, 59 interactors |
CORUMi | P78395 |
IntActi | P78395, 32 interactors |
MINTi | P78395 |
STRINGi | 9606.ENSP00000445675 |
PTM databases
iPTMneti | P78395 |
PhosphoSitePlusi | P78395 |
Genetic variation databases
BioMutai | PRAME |
DMDMi | 6685631 |
Proteomic databases
EPDi | P78395 |
jPOSTi | P78395 |
MassIVEi | P78395 |
MaxQBi | P78395 |
PaxDbi | P78395 |
PeptideAtlasi | P78395 |
PRIDEi | P78395 |
ProteomicsDBi | 57611 |
Protocols and materials databases
Antibodypediai | 23630, 442 antibodies |
DNASUi | 23532 |
Genome annotation databases
Organism-specific databases
CTDi | 23532 |
DisGeNETi | 23532 |
GeneCardsi | PRAME |
HGNCi | HGNC:9336, PRAME |
HPAi | ENSG00000185686, Tissue enriched (testis) |
MIMi | 606021, gene |
neXtProti | NX_P78395 |
OpenTargetsi | ENSG00000185686 |
PharmGKBi | PA33698 |
VEuPathDBi | HostDB:ENSG00000185686.17 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QWSJ, Eukaryota |
GeneTreei | ENSGT00990000203552 |
HOGENOMi | CLU_039635_2_1_1 |
InParanoidi | P78395 |
OMAi | TFTFCGN |
OrthoDBi | 631249at2759 |
PhylomeDBi | P78395 |
TreeFami | TF332708 |
Enzyme and pathway databases
PathwayCommonsi | P78395 |
SIGNORi | P78395 |
Miscellaneous databases
BioGRID-ORCSi | 23532, 22 hits in 999 CRISPR screens |
ChiTaRSi | PRAME, human |
GeneWikii | PRAME |
GenomeRNAii | 23532 |
Pharosi | P78395, Tbio |
PROi | PR:P78395 |
RNActi | P78395, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000185686, Expressed in right testis and 91 other tissues |
ExpressionAtlasi | P78395, baseline and differential |
Genevisiblei | P78395, HS |
Family and domain databases
Gene3Di | 3.80.10.10, 1 hit |
InterProi | View protein in InterPro IPR032675, LRR_dom_sf IPR026271, PRAME_family |
PIRSFi | PIRSF038286, PRAME, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRAME_HUMAN | |
Accessioni | P78395Primary (citable) accession number: P78395 Secondary accession number(s): B2R6Y7, O43481, Q8IXN8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | May 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 171 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 22
Human chromosome 22: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families