UniProtKB - P78371 (TCPB_HUMAN)
Protein
T-complex protein 1 subunit beta
Gene
CCT2
Organism
Homo sapiens (Human)
Status
Functioni
Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis (PubMed:25467444). The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance (PubMed:25467444). As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia (PubMed:20080638). The TRiC complex plays a role in the folding of actin and tubulin (Probable).Curated2 Publications
GO - Molecular functioni
- ATPase activity Source: InterPro
- ATP binding Source: UniProtKB-KW
- protein folding chaperone Source: BHF-UCL
- ubiquitin protein ligase binding Source: ParkinsonsUK-UCL
- unfolded protein binding Source: GO_Central
GO - Biological processi
- binding of sperm to zona pellucida Source: Ensembl
- chaperone-mediated protein complex assembly Source: MGI
- chaperone mediated protein folding independent of cofactor Source: BHF-UCL
- neutrophil degranulation Source: Reactome
- positive regulation of establishment of protein localization to telomere Source: BHF-UCL
- positive regulation of protein localization to Cajal body Source: BHF-UCL
- positive regulation of telomerase activity Source: BHF-UCL
- positive regulation of telomerase RNA localization to Cajal body Source: BHF-UCL
- positive regulation of telomere maintenance via telomerase Source: BHF-UCL
- protein folding Source: FlyBase
- protein stabilization Source: BHF-UCL
- scaRNA localization to Cajal body Source: BHF-UCL
- toxin transport Source: Ensembl
Keywordsi
Molecular function | Chaperone |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | P78371 |
Reactomei | R-HSA-389957, Prefoldin mediated transfer of substrate to CCT/TriC R-HSA-389960, Formation of tubulin folding intermediates by CCT/TriC R-HSA-390450, Folding of actin by CCT/TriC R-HSA-390471, Association of TriC/CCT with target proteins during biosynthesis R-HSA-5620922, BBSome-mediated cargo-targeting to cilium R-HSA-6798695, Neutrophil degranulation R-HSA-6814122, Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding |
SIGNORi | P78371 |
Names & Taxonomyi
Protein namesi | Recommended name: T-complex protein 1 subunit betaShort name: TCP-1-beta Alternative name(s): CCT-beta |
Gene namesi | Name:CCT2 Synonyms:99D8.1, CCTB |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:1615, CCT2 |
MIMi | 605139, gene |
neXtProti | NX_P78371 |
VEuPathDBi | HostDB:ENSG00000166226.12 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytoskeleton
- microtubule Source: UniProtKB
Cytosol
- chaperonin-containing T-complex Source: UniProtKB
- cytosol Source: HPA
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
- extracellular region Source: Reactome
Lysosome
- azurophil granule lumen Source: Reactome
Other locations
- cell body Source: Ensembl
- zona pellucida receptor complex Source: Ensembl
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Organism-specific databases
DisGeNETi | 10576 |
OpenTargetsi | ENSG00000166226 |
PharmGKBi | PA26179 |
Miscellaneous databases
Pharosi | P78371, Tbio |
Genetic variation databases
BioMutai | CCT2 |
DMDMi | 6094436 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources2 Publications | |||
ChainiPRO_0000128316 | 2 – 535 | T-complex protein 1 subunit betaAdd BLAST | 534 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources1 Publication | 1 | ||
Modified residuei | 3 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 13 | N6-acetyllysineCombined sources | 1 | ||
Modified residuei | 60 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 154 | N6-acetyllysineCombined sources | 1 | ||
Modified residuei | 181 | N6-acetyllysineCombined sources | 1 | ||
Cross-linki | 248 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources | |||
Modified residuei | 260 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 261 | PhosphothreonineCombined sources | 1 | ||
Isoform 2 (identifier: P78371-2) | |||||
Modified residuei | 1 | N-acetylmethionineCombined sources | 1 |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
CPTACi | CPTAC-39 CPTAC-40 |
EPDi | P78371 |
jPOSTi | P78371 |
MassIVEi | P78371 |
PaxDbi | P78371 |
PeptideAtlasi | P78371 |
PRIDEi | P78371 |
ProteomicsDBi | 57599 [P78371-1] 57600 [P78371-2] |
TopDownProteomicsi | P78371-1 [P78371-1] |
2D gel databases
OGPi | P78371 |
REPRODUCTION-2DPAGEi | IPI00297779 |
SWISS-2DPAGEi | P78371 |
UCD-2DPAGEi | P78371 |
PTM databases
iPTMneti | P78371 |
MetOSitei | P78371 |
PhosphoSitePlusi | P78371 |
SwissPalmi | P78371 |
Expressioni
Gene expression databases
Bgeei | ENSG00000166226, Expressed in testis and 245 other tissues |
ExpressionAtlasi | P78371, baseline and differential |
Genevisiblei | P78371, HS |
Organism-specific databases
HPAi | ENSG00000166226, Low tissue specificity |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsP78371
GO - Molecular functioni
- ubiquitin protein ligase binding Source: ParkinsonsUK-UCL
- unfolded protein binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 115827, 452 interactors |
ComplexPortali | CPX-6030, Chaperonin-containing T-complex |
CORUMi | P78371 |
DIPi | DIP-38123N |
IntActi | P78371, 256 interactors |
MINTi | P78371 |
STRINGi | 9606.ENSP00000299300 |
Miscellaneous databases
RNActi | P78371, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the TCP-1 chaperonin family.Curated
Phylogenomic databases
eggNOGi | KOG0363, Eukaryota |
GeneTreei | ENSGT00550000074930 |
InParanoidi | P78371 |
OMAi | HQPQRIE |
PhylomeDBi | P78371 |
TreeFami | TF105645 |
Family and domain databases
CDDi | cd03336, TCP1_beta, 1 hit |
Gene3Di | 1.10.560.10, 1 hit 3.30.260.10, 1 hit 3.50.7.10, 1 hit |
InterProi | View protein in InterPro IPR012716, Chap_CCT_beta IPR017998, Chaperone_TCP-1 IPR002194, Chaperonin_TCP-1_CS IPR002423, Cpn60/TCP-1 IPR027409, GroEL-like_apical_dom_sf IPR027413, GROEL-like_equatorial_sf IPR027410, TCP-1-like_intermed_sf |
PANTHERi | PTHR11353:SF23, PTHR11353:SF23, 1 hit |
Pfami | View protein in Pfam PF00118, Cpn60_TCP1, 1 hit |
PRINTSi | PR00304, TCOMPLEXTCP1 |
SUPFAMi | SSF48592, SSF48592, 1 hit SSF52029, SSF52029, 1 hit SSF54849, SSF54849, 1 hit |
TIGRFAMsi | TIGR02341, chap_CCT_beta, 1 hit |
PROSITEi | View protein in PROSITE PS00750, TCP1_1, 1 hit PS00751, TCP1_2, 1 hit PS00995, TCP1_3, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P78371-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASLSLAPVN IFKAGADEER AETARLTSFI GAIAIGDLVK STLGPKGMDK
60 70 80 90 100
ILLSSGRDAS LMVTNDGATI LKNIGVDNPA AKVLVDMSRV QDDEVGDGTT
110 120 130 140 150
SVTVLAAELL REAESLIAKK IHPQTIIAGW REATKAAREA LLSSAVDHGS
160 170 180 190 200
DEVKFRQDLM NIAGTTLSSK LLTHHKDHFT KLAVEAVLRL KGSGNLEAIH
210 220 230 240 250
IIKKLGGSLA DSYLDEGFLL DKKIGVNQPK RIENAKILIA NTGMDTDKIK
260 270 280 290 300
IFGSRVRVDS TAKVAEIEHA EKEKMKEKVE RILKHGINCF INRQLIYNYP
310 320 330 340 350
EQLFGAAGVM AIEHADFAGV ERLALVTGGE IASTFDHPEL VKLGSCKLIE
360 370 380 390 400
EVMIGEDKLI HFSGVALGEA CTIVLRGATQ QILDEAERSL HDALCVLAQT
410 420 430 440 450
VKDSRTVYGG GCSEMLMAHA VTQLANRTPG KEAVAMESYA KALRMLPTII
460 470 480 490 500
ADNAGYDSAD LVAQLRAAHS EGNTTAGLDM REGTIGDMAI LGITESFQVK
510 520 530
RQVLLSAAEA AEVILRVDNI IKAAPRKRVP DHHPC
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF5GWF6 | F5GWF6_HUMAN | CCT-beta | CCT2 | 530 | Annotation score: | ||
F8VQ14 | F8VQ14_HUMAN | CCT-beta | CCT2 | 416 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 51 | I → T in BAH11729 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 126 | I → T in CAG33352 (Ref. 5) Curated | 1 | |
Sequence conflicti | 354 | I → T in BAF83456 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 438 | S → P in BAH13640 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 444 | R → K in BAG70037 (PubMed:19054851).Curated | 1 | |
Sequence conflicti | 444 | R → K in BAG70160 (PubMed:19054851).Curated | 1 | |
Sequence conflicti | 504 | L → P in CAG33352 (Ref. 5) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_042648 | 1 – 47 | Missing in isoform 2. 1 PublicationAdd BLAST | 47 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF026293 mRNA Translation: AAC96012.1 AF026166 mRNA Translation: AAC98906.1 BT019966 mRNA Translation: AAV38769.1 AK290767 mRNA Translation: BAF83456.1 AK294307 mRNA Translation: BAH11729.1 AK302157 mRNA Translation: BAH13640.1 AK316397 mRNA Translation: BAH14768.1 AK316408 mRNA Translation: BAH14779.1 CR457071 mRNA Translation: CAG33352.1 AB451223 mRNA Translation: BAG70037.1 AB451346 mRNA Translation: BAG70160.1 AC018921 Genomic DNA No translation available. CH471054 Genomic DNA Translation: EAW97230.1 BC113514 mRNA Translation: AAI13515.1 BC113516 mRNA Translation: AAI13517.1 U91327 Genomic DNA Translation: AAB67249.1 |
CCDSi | CCDS55843.1 [P78371-2] CCDS8991.1 [P78371-1] |
RefSeqi | NP_001185771.1, NM_001198842.1 [P78371-2] NP_006422.1, NM_006431.2 [P78371-1] |
Genome annotation databases
Ensembli | ENST00000299300; ENSP00000299300; ENSG00000166226 [P78371-1] ENST00000543146; ENSP00000445471; ENSG00000166226 [P78371-2] |
GeneIDi | 10576 |
KEGGi | hsa:10576 |
UCSCi | uc010stl.2, human [P78371-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF026293 mRNA Translation: AAC96012.1 AF026166 mRNA Translation: AAC98906.1 BT019966 mRNA Translation: AAV38769.1 AK290767 mRNA Translation: BAF83456.1 AK294307 mRNA Translation: BAH11729.1 AK302157 mRNA Translation: BAH13640.1 AK316397 mRNA Translation: BAH14768.1 AK316408 mRNA Translation: BAH14779.1 CR457071 mRNA Translation: CAG33352.1 AB451223 mRNA Translation: BAG70037.1 AB451346 mRNA Translation: BAG70160.1 AC018921 Genomic DNA No translation available. CH471054 Genomic DNA Translation: EAW97230.1 BC113514 mRNA Translation: AAI13515.1 BC113516 mRNA Translation: AAI13517.1 U91327 Genomic DNA Translation: AAB67249.1 |
CCDSi | CCDS55843.1 [P78371-2] CCDS8991.1 [P78371-1] |
RefSeqi | NP_001185771.1, NM_001198842.1 [P78371-2] NP_006422.1, NM_006431.2 [P78371-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6NR8 | electron microscopy | 7.80 | B/J | 16-524 | [»] | |
6NR9 | electron microscopy | 8.50 | B/J | 16-524 | [»] | |
6NRA | electron microscopy | 7.70 | B/J | 16-524 | [»] | |
6NRB | electron microscopy | 8.70 | B/J | 16-524 | [»] | |
6NRC | electron microscopy | 8.30 | B/J | 16-524 | [»] | |
6NRD | electron microscopy | 8.20 | B/J | 16-524 | [»] | |
6QB8 | electron microscopy | 3.97 | B/b | 1-535 | [»] | |
SMRi | P78371 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115827, 452 interactors |
ComplexPortali | CPX-6030, Chaperonin-containing T-complex |
CORUMi | P78371 |
DIPi | DIP-38123N |
IntActi | P78371, 256 interactors |
MINTi | P78371 |
STRINGi | 9606.ENSP00000299300 |
PTM databases
iPTMneti | P78371 |
MetOSitei | P78371 |
PhosphoSitePlusi | P78371 |
SwissPalmi | P78371 |
Genetic variation databases
BioMutai | CCT2 |
DMDMi | 6094436 |
2D gel databases
OGPi | P78371 |
REPRODUCTION-2DPAGEi | IPI00297779 |
SWISS-2DPAGEi | P78371 |
UCD-2DPAGEi | P78371 |
Proteomic databases
CPTACi | CPTAC-39 CPTAC-40 |
EPDi | P78371 |
jPOSTi | P78371 |
MassIVEi | P78371 |
PaxDbi | P78371 |
PeptideAtlasi | P78371 |
PRIDEi | P78371 |
ProteomicsDBi | 57599 [P78371-1] 57600 [P78371-2] |
TopDownProteomicsi | P78371-1 [P78371-1] |
Protocols and materials databases
Antibodypediai | 762, 352 antibodies |
DNASUi | 10576 |
Genome annotation databases
Ensembli | ENST00000299300; ENSP00000299300; ENSG00000166226 [P78371-1] ENST00000543146; ENSP00000445471; ENSG00000166226 [P78371-2] |
GeneIDi | 10576 |
KEGGi | hsa:10576 |
UCSCi | uc010stl.2, human [P78371-1] |
Organism-specific databases
CTDi | 10576 |
DisGeNETi | 10576 |
GeneCardsi | CCT2 |
HGNCi | HGNC:1615, CCT2 |
HPAi | ENSG00000166226, Low tissue specificity |
MIMi | 605139, gene |
neXtProti | NX_P78371 |
OpenTargetsi | ENSG00000166226 |
PharmGKBi | PA26179 |
VEuPathDBi | HostDB:ENSG00000166226.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0363, Eukaryota |
GeneTreei | ENSGT00550000074930 |
InParanoidi | P78371 |
OMAi | HQPQRIE |
PhylomeDBi | P78371 |
TreeFami | TF105645 |
Enzyme and pathway databases
PathwayCommonsi | P78371 |
Reactomei | R-HSA-389957, Prefoldin mediated transfer of substrate to CCT/TriC R-HSA-389960, Formation of tubulin folding intermediates by CCT/TriC R-HSA-390450, Folding of actin by CCT/TriC R-HSA-390471, Association of TriC/CCT with target proteins during biosynthesis R-HSA-5620922, BBSome-mediated cargo-targeting to cilium R-HSA-6798695, Neutrophil degranulation R-HSA-6814122, Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding |
SIGNORi | P78371 |
Miscellaneous databases
BioGRID-ORCSi | 10576, 764 hits in 983 CRISPR screens |
ChiTaRSi | CCT2, human |
GeneWikii | CCT2_(gene) |
GenomeRNAii | 10576 |
Pharosi | P78371, Tbio |
PROi | PR:P78371 |
RNActi | P78371, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000166226, Expressed in testis and 245 other tissues |
ExpressionAtlasi | P78371, baseline and differential |
Genevisiblei | P78371, HS |
Family and domain databases
CDDi | cd03336, TCP1_beta, 1 hit |
Gene3Di | 1.10.560.10, 1 hit 3.30.260.10, 1 hit 3.50.7.10, 1 hit |
InterProi | View protein in InterPro IPR012716, Chap_CCT_beta IPR017998, Chaperone_TCP-1 IPR002194, Chaperonin_TCP-1_CS IPR002423, Cpn60/TCP-1 IPR027409, GroEL-like_apical_dom_sf IPR027413, GROEL-like_equatorial_sf IPR027410, TCP-1-like_intermed_sf |
PANTHERi | PTHR11353:SF23, PTHR11353:SF23, 1 hit |
Pfami | View protein in Pfam PF00118, Cpn60_TCP1, 1 hit |
PRINTSi | PR00304, TCOMPLEXTCP1 |
SUPFAMi | SSF48592, SSF48592, 1 hit SSF52029, SSF52029, 1 hit SSF54849, SSF54849, 1 hit |
TIGRFAMsi | TIGR02341, chap_CCT_beta, 1 hit |
PROSITEi | View protein in PROSITE PS00750, TCP1_1, 1 hit PS00751, TCP1_2, 1 hit PS00995, TCP1_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TCPB_HUMAN | |
Accessioni | P78371Primary (citable) accession number: P78371 Secondary accession number(s): A8K402 Q6IAT3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 202 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 12
Human chromosome 12: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families