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Protein

Phosphatidylinositol 5-phosphate 4-kinase type-2 beta

Gene

PIP4K2B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate.1 Publication

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol 5-phosphate = ADP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate.

Kineticsi

  1. KM=30 µM for phosphatidylinositol-5- phosphate1 Publication
  1. Vmax=0.2 pmol/min/µg enzyme1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.149 2681
ReactomeiR-HSA-1660499 Synthesis of PIPs at the plasma membrane
R-HSA-6811555 PI5P Regulates TP53 Acetylation
R-HSA-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling
R-HSA-8847453 Synthesis of PIPs in the nucleus
SABIO-RKiP78356
SIGNORiP78356

Chemistry databases

SwissLipidsiSLP:000001885

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 5-phosphate 4-kinase type-2 beta (EC:2.7.1.149)
Alternative name(s):
1-phosphatidylinositol 5-phosphate 4-kinase 2-beta
Diphosphoinositide kinase 2-beta
Phosphatidylinositol 5-phosphate 4-kinase type II beta
Short name:
PI(5)P 4-kinase type II beta
Short name:
PIP4KII-beta
PtdIns(5)P-4-kinase isoform 2-beta
Gene namesi
Name:PIP4K2B
Synonyms:PIP5K2B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000276293.4
HGNCiHGNC:8998 PIP4K2B
MIMi603261 gene
neXtProtiNX_P78356

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi8396
OpenTargetsiENSG00000276293
PharmGKBiPA162399640

Chemistry databases

ChEMBLiCHEMBL5667

Polymorphism and mutation databases

BioMutaiPIP4K2B
DMDMi47605991

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001854702 – 416Phosphatidylinositol 5-phosphate 4-kinase type-2 betaAdd BLAST415

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei8PhosphothreonineBy similarity1
Modified residuei19PhosphoserineBy similarity1
Modified residuei94N6-acetyllysineBy similarity1
Modified residuei150N6-acetyllysineCombined sources1
Modified residuei322PhosphothreonineBy similarity1
Modified residuei326PhosphoserineCombined sources1

Post-translational modificationi

Ubiquitinated by the SPOP/CUL3 complex. Ubiquitination is stimulated by PtdIns5P levels.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP78356
MaxQBiP78356
PaxDbiP78356
PeptideAtlasiP78356
PRIDEiP78356
ProteomicsDBi57586
57587 [P78356-2]

PTM databases

iPTMnetiP78356
PhosphoSitePlusiP78356

Expressioni

Tissue specificityi

Highly expressed in brain, heart, pancreas, skeletal muscle and kidney. Detected at lower levels in placenta, lung and liver.1 Publication

Gene expression databases

BgeeiENSG00000276293 Expressed in 233 organ(s), highest expression level in cerebral cortex
CleanExiHS_PIP4K2B
ExpressionAtlasiP78356 baseline and differential
GenevisibleiP78356 HS

Organism-specific databases

HPAiHPA047875
HPA062220

Interactioni

Subunit structurei

Homodimer. Binds TNFRSF1A. Interacts with PIP4K2A. Interaction with PIP4K2A suppresses ubiquitination by the SPOP/ CUL3 complex.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
HMBOX1Q6NT765EBI-947090,EBI-2549423

GO - Molecular functioni

Protein-protein interaction databases

BioGridi113985, 38 interactors
IntActiP78356, 18 interactors
STRINGi9606.ENSP00000269554

Chemistry databases

BindingDBiP78356

Structurei

Secondary structure

1416
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

DisProtiDP00054
ProteinModelPortaliP78356
SMRiP78356
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP78356

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 415PIPKPROSITE-ProRule annotationAdd BLAST378

Phylogenomic databases

eggNOGiKOG0229 Eukaryota
COG5253 LUCA
GeneTreeiENSGT00760000119184
HOGENOMiHOG000007832
HOVERGENiHBG000072
InParanoidiP78356
KOiK00920
OMAiECENDGL
OrthoDBiEOG091G0857
PhylomeDBiP78356
TreeFamiTF354315

Family and domain databases

Gene3Di3.30.800.10, 1 hit
3.30.810.10, 2 hits
InterProiView protein in InterPro
IPR023610 PInositol-4-P-5-kinase
IPR027483 PInositol-4-P-5-kinase_C
IPR002498 PInositol-4-P-5-kinase_core
IPR027484 PInositol-4-P-5-kinase_N
PANTHERiPTHR23086 PTHR23086, 1 hit
PfamiView protein in Pfam
PF01504 PIP5K, 1 hit
SMARTiView protein in SMART
SM00330 PIPKc, 1 hit
PROSITEiView protein in PROSITE
PS51455 PIPK, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: P78356-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSSNCTSTTA VAVAPLSASK TKTKKKHFVC QKVKLFRASE PILSVLMWGV
60 70 80 90 100
NHTINELSNV PVPVMLMPDD FKAYSKIKVD NHLFNKENLP SRFKFKEYCP
110 120 130 140 150
MVFRNLRERF GIDDQDYQNS VTRSAPINSD SQGRCGTRFL TTYDRRFVIK
160 170 180 190 200
TVSSEDVAEM HNILKKYHQF IVECHGNTLL PQFLGMYRLT VDGVETYMVV
210 220 230 240 250
TRNVFSHRLT VHRKYDLKGS TVAREASDKE KAKDLPTFKD NDFLNEGQKL
260 270 280 290 300
HVGEESKKNF LEKLKRDVEF LAQLKIMDYS LLVGIHDVDR AEQEEMEVEE
310 320 330 340 350
RAEDEECEND GVGGNLLCSY GTPPDSPGNL LSFPRFFGPG EFDPSVDVYA
360 370 380 390 400
MKSHESSPKK EVYFMAIIDI LTPYDTKKKA AHAAKTVKHG AGAEISTVNP
410
EQYSKRFNEF MSNILT
Length:416
Mass (Da):47,378
Last modified:May 1, 1997 - v1
Checksum:i2288CD2883EACEBE
GO
Isoform 2 (identifier: P78356-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     270-281: FLAQLKIMDYSL → EILVLSPGRRIA
     282-416: Missing.

Note: No experimental confirmation available.
Show »
Length:281
Mass (Da):32,317
Checksum:iD83D7A24BC4B8DA6
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WT35A0A087WT35_HUMAN
Phosphatidylinositol 5-phosphate 4-...
PIP4K2B
71Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti250L → P in AAV38420 (Ref. 2) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010384270 – 281FLAQL…MDYSL → EILVLSPGRRIA in isoform 2. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_010385282 – 416Missing in isoform 2. 1 PublicationAdd BLAST135

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U85245 mRNA Translation: AAB48596.1
BT019614 mRNA Translation: AAV38420.1
BC027459 mRNA Translation: AAH27459.1
CCDSiCCDS11329.1 [P78356-1]
RefSeqiNP_003550.1, NM_003559.4 [P78356-1]
UniGeneiHs.260603

Genome annotation databases

EnsembliENST00000613180; ENSP00000480776; ENSG00000277292 [P78356-1]
ENST00000619039; ENSP00000482548; ENSG00000276293 [P78356-1]
GeneIDi8396
KEGGihsa:8396
UCSCiuc002hqs.4 human [P78356-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U85245 mRNA Translation: AAB48596.1
BT019614 mRNA Translation: AAV38420.1
BC027459 mRNA Translation: AAH27459.1
CCDSiCCDS11329.1 [P78356-1]
RefSeqiNP_003550.1, NM_003559.4 [P78356-1]
UniGeneiHs.260603

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BO1X-ray3.00A/B1-416[»]
3WZZX-ray2.60A/B31-416[»]
3X01X-ray2.15A/B31-416[»]
3X02X-ray2.45A/B31-416[»]
3X03X-ray2.70A/B31-416[»]
3X04X-ray2.60A/B31-416[»]
3X05X-ray2.50A/B31-416[»]
3X06X-ray2.65A/B31-416[»]
3X07X-ray2.60A/B31-416[»]
3X08X-ray2.75A/B31-416[»]
3X09X-ray2.70A/B31-416[»]
3X0AX-ray2.60A/B31-416[»]
3X0BX-ray2.60A/B31-416[»]
3X0CX-ray2.55A/B31-416[»]
DisProtiDP00054
ProteinModelPortaliP78356
SMRiP78356
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113985, 38 interactors
IntActiP78356, 18 interactors
STRINGi9606.ENSP00000269554

Chemistry databases

BindingDBiP78356
ChEMBLiCHEMBL5667
SwissLipidsiSLP:000001885

PTM databases

iPTMnetiP78356
PhosphoSitePlusiP78356

Polymorphism and mutation databases

BioMutaiPIP4K2B
DMDMi47605991

Proteomic databases

EPDiP78356
MaxQBiP78356
PaxDbiP78356
PeptideAtlasiP78356
PRIDEiP78356
ProteomicsDBi57586
57587 [P78356-2]

Protocols and materials databases

DNASUi8396
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000613180; ENSP00000480776; ENSG00000277292 [P78356-1]
ENST00000619039; ENSP00000482548; ENSG00000276293 [P78356-1]
GeneIDi8396
KEGGihsa:8396
UCSCiuc002hqs.4 human [P78356-1]

Organism-specific databases

CTDi8396
DisGeNETi8396
EuPathDBiHostDB:ENSG00000276293.4
GeneCardsiPIP4K2B
HGNCiHGNC:8998 PIP4K2B
HPAiHPA047875
HPA062220
MIMi603261 gene
neXtProtiNX_P78356
OpenTargetsiENSG00000276293
PharmGKBiPA162399640
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0229 Eukaryota
COG5253 LUCA
GeneTreeiENSGT00760000119184
HOGENOMiHOG000007832
HOVERGENiHBG000072
InParanoidiP78356
KOiK00920
OMAiECENDGL
OrthoDBiEOG091G0857
PhylomeDBiP78356
TreeFamiTF354315

Enzyme and pathway databases

BRENDAi2.7.1.149 2681
ReactomeiR-HSA-1660499 Synthesis of PIPs at the plasma membrane
R-HSA-6811555 PI5P Regulates TP53 Acetylation
R-HSA-6811558 PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling
R-HSA-8847453 Synthesis of PIPs in the nucleus
SABIO-RKiP78356
SIGNORiP78356

Miscellaneous databases

ChiTaRSiPIP4K2B human
EvolutionaryTraceiP78356
GeneWikiiPIP4K2B
GenomeRNAii8396
PROiPR:P78356
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000276293 Expressed in 233 organ(s), highest expression level in cerebral cortex
CleanExiHS_PIP4K2B
ExpressionAtlasiP78356 baseline and differential
GenevisibleiP78356 HS

Family and domain databases

Gene3Di3.30.800.10, 1 hit
3.30.810.10, 2 hits
InterProiView protein in InterPro
IPR023610 PInositol-4-P-5-kinase
IPR027483 PInositol-4-P-5-kinase_C
IPR002498 PInositol-4-P-5-kinase_core
IPR027484 PInositol-4-P-5-kinase_N
PANTHERiPTHR23086 PTHR23086, 1 hit
PfamiView protein in Pfam
PF01504 PIP5K, 1 hit
SMARTiView protein in SMART
SM00330 PIPKc, 1 hit
PROSITEiView protein in PROSITE
PS51455 PIPK, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPI42B_HUMAN
AccessioniPrimary (citable) accession number: P78356
Secondary accession number(s): Q5U0E8, Q8TBP2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 1, 1997
Last modified: September 12, 2018
This is version 163 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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