UniProtKB - P78346 (RPP30_HUMAN)
Ribonuclease P protein subunit p30
RPP30
Functioni
Miscellaneous
Catalytic activityi
- Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.3 Publications EC:3.1.26.5
GO - Molecular functioni
- ribonuclease P activity Source: ProtInc
- ribonuclease P RNA binding Source: UniProtKB
- RNA binding Source: UniProtKB
GO - Biological processi
- RNA phosphodiester bond hydrolysis, endonucleolytic Source: GO_Central
- rRNA processing Source: UniProtKB-KW
- tRNA 5'-leader removal Source: UniProtKB
- tRNA processing Source: GO_Central
Keywordsi
Molecular function | Hydrolase, RNA-binding |
Biological process | rRNA processing, tRNA processing |
Enzyme and pathway databases
PathwayCommonsi | P78346 |
Reactomei | R-HSA-6784531, tRNA processing in the nucleus R-HSA-6791226, Major pathway of rRNA processing in the nucleolus and cytosol |
Names & Taxonomyi
Protein namesi | Recommended name: Ribonuclease P protein subunit p30 (EC:3.1.26.53 Publications)Short name: RNaseP protein p30 Alternative name(s): RNase P subunit 2 |
Gene namesi | Name:RPP30 Synonyms:RNASEP2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:17688, RPP30 |
MIMi | 606115, gene |
neXtProti | NX_P78346 |
VEuPathDBi | HostDB:ENSG00000148688.13 |
Subcellular locationi
Nucleus
- nucleolus Curated
Nucleus
- nucleolar ribonuclease P complex Source: GO_Central
- nucleoplasm Source: Reactome
- nucleus Source: ProtInc
Other locations
- multimeric ribonuclease P complex Source: UniProtKB
- ribonuclease MRP complex Source: FlyBase
Keywords - Cellular componenti
NucleusPathology & Biotechi
Organism-specific databases
OpenTargetsi | ENSG00000148688 |
PharmGKBi | PA134876345 |
Miscellaneous databases
Pharosi | P78346, Tbio |
Genetic variation databases
BioMutai | RPP30 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources2 Publications | |||
ChainiPRO_0000140031 | 2 – 268 | Ribonuclease P protein subunit p30Add BLAST | 267 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources2 Publications | 1 | |
Modified residuei | 251 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | P78346 |
jPOSTi | P78346 |
MassIVEi | P78346 |
MaxQBi | P78346 |
PaxDbi | P78346 |
PeptideAtlasi | P78346 |
PRIDEi | P78346 |
ProteomicsDBi | 19113 57577 [P78346-1] |
TopDownProteomicsi | P78346-1 [P78346-1] |
PTM databases
iPTMneti | P78346 |
PhosphoSitePlusi | P78346 |
SwissPalmi | P78346 |
Expressioni
Gene expression databases
Bgeei | ENSG00000148688, Expressed in testis and 239 other tissues |
ExpressionAtlasi | P78346, baseline and differential |
Genevisiblei | P78346, HS |
Organism-specific databases
HPAi | ENSG00000148688, Low tissue specificity |
Interactioni
Subunit structurei
Component of nuclear RNase P and RNase MRP ribonucleoproteins (PubMed:9037013, PubMed:9630247, PubMed:16723659, PubMed:30454648). RNase P consists of a catalytic RNA moiety and about 10 protein subunits; POP1, POP4, POP5, POP7, RPP14, RPP21, RPP25, RPP30, RPP38 and RPP40 (PubMed:9037013, PubMed:9630247, PubMed:16723659, PubMed:30454648). Within the RNase P complex, POP1, POP7 and RPP25 form the 'finger' subcomplex, POP5, RPP14, RPP40 and homodimeric RPP30 form the 'palm' subcomplex, and RPP21, POP4 and RPP38 form the 'wrist' subcomplex. All subunits of the RNase P complex interact with the catalytic RNA (PubMed:30454648). Several subunits of RNase P are also part of the RNase MRP complex. RNase MRP consists of a catalytic RNA moiety and about 8 protein subunits; POP1, POP7, RPP25, RPP30, RPP38, RPP40 and possibly also POP4 and POP5 (PubMed:16723659, PubMed:28115465).
5 PublicationsBinary interactionsi
Hide detailsP78346
With | #Exp. | IntAct |
---|---|---|
HMG20A [Q9NP66] | 11 | EBI-366553,EBI-740641 |
POP5 [Q969H6] | 7 | EBI-366553,EBI-366525 |
RPP14 [O95059] | 5 | EBI-366553,EBI-366542 |
Protein-protein interaction databases
BioGRIDi | 115807, 57 interactors |
CORUMi | P78346 |
IntActi | P78346, 33 interactors |
MINTi | P78346 |
STRINGi | 9606.ENSP00000389182 |
Miscellaneous databases
RNActi | P78346, protein |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2363, Eukaryota |
GeneTreei | ENSGT00390000000883 |
InParanoidi | P78346 |
OMAi | PWDVINL |
OrthoDBi | 1197518at2759 |
PhylomeDBi | P78346 |
TreeFami | TF106113 |
Family and domain databases
InterProi | View protein in InterPro IPR016195, Pol/histidinol_Pase-like IPR002738, RNase_P_p30 |
PANTHERi | PTHR13031, PTHR13031, 1 hit |
Pfami | View protein in Pfam PF01876, RNase_P_p30, 1 hit |
SUPFAMi | SSF89550, SSF89550, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAVFADLDLR AGSDLKALRG LVETAAHLGY SVVAINHIVD FKEKKQEIEK
60 70 80 90 100
PVAVSELFTT LPIVQGKSRP IKILTRLTII VSDPSHCNVL RATSSRARLY
110 120 130 140 150
DVVAVFPKTE KLFHIACTHL DVDLVCITVT EKLPFYFKRP PINVAIDRGL
160 170 180 190 200
AFELVYSPAI KDSTMRRYTI SSALNLMQIC KGKNVIISSA AERPLEIRGP
210 220 230 240 250
YDVANLGLLF GLSESDAKAA VSTNCRAALL HGETRKTAFG IISTVKKPRP
260
SEGDEDCLPA SKKAKCEG
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ5VU10 | Q5VU10_HUMAN | Ribonuclease P protein subunit p30 | RPP30 | 212 | Annotation score: | ||
Q5VU11 | Q5VU11_HUMAN | Ribonuclease P protein subunit p30 | RPP30 | 254 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Sequence conflicti | 65 | Q → R in AK225532 (Ref. 3) Curated | 1 | ||
Sequence conflicti | 189 | S → I AA sequence (PubMed:9037013).Curated | 1 | ||
Isoform 2 (identifier: P78346-2) | |||||
Sequence conflicti | 291 | K → R in AK225532 (Ref. 3) Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_051870 | 12 | G → D. Corresponds to variant dbSNP:rs11544145Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_045673 | 266 – 268 | CEG → WSHSVTQAGVQWHNLGSLQP LPLGLKPSSHLSLPRTRIQQ RQLLISHQRDHTPKNRL in isoform 2. 1 Publication | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U77665 mRNA Translation: AAC51143.1 AK312900 mRNA Translation: BAG35746.1 AK225532 mRNA No translation available. AL590622 Genomic DNA No translation available. CH471066 Genomic DNA Translation: EAW50117.1 BC006991 mRNA Translation: AAH06991.1 |
CCDSi | CCDS44458.1 [P78346-2] CCDS7411.1 [P78346-1] |
RefSeqi | NP_001098016.1, NM_001104546.1 [P78346-2] NP_006404.1, NM_006413.4 [P78346-1] XP_011537422.1, XM_011539120.2 XP_011537423.1, XM_011539121.2 XP_016870964.1, XM_017015475.1 XP_016870966.1, XM_017015477.1 |
Genome annotation databases
Ensembli | ENST00000371703; ENSP00000360768; ENSG00000148688 [P78346-1] ENST00000413330; ENSP00000389182; ENSG00000148688 [P78346-2] |
GeneIDi | 10556 |
KEGGi | hsa:10556 |
UCSCi | uc009xtx.4, human [P78346-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U77665 mRNA Translation: AAC51143.1 AK312900 mRNA Translation: BAG35746.1 AK225532 mRNA No translation available. AL590622 Genomic DNA No translation available. CH471066 Genomic DNA Translation: EAW50117.1 BC006991 mRNA Translation: AAH06991.1 |
CCDSi | CCDS44458.1 [P78346-2] CCDS7411.1 [P78346-1] |
RefSeqi | NP_001098016.1, NM_001104546.1 [P78346-2] NP_006404.1, NM_006413.4 [P78346-1] XP_011537422.1, XM_011539120.2 XP_011537423.1, XM_011539121.2 XP_016870964.1, XM_017015475.1 XP_016870966.1, XM_017015477.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6AHR | electron microscopy | 3.92 | I/J | 1-268 | [»] | |
6AHU | electron microscopy | 3.66 | I/J | 1-268 | [»] | |
SMRi | P78346 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 115807, 57 interactors |
CORUMi | P78346 |
IntActi | P78346, 33 interactors |
MINTi | P78346 |
STRINGi | 9606.ENSP00000389182 |
PTM databases
iPTMneti | P78346 |
PhosphoSitePlusi | P78346 |
SwissPalmi | P78346 |
Genetic variation databases
BioMutai | RPP30 |
Proteomic databases
EPDi | P78346 |
jPOSTi | P78346 |
MassIVEi | P78346 |
MaxQBi | P78346 |
PaxDbi | P78346 |
PeptideAtlasi | P78346 |
PRIDEi | P78346 |
ProteomicsDBi | 19113 57577 [P78346-1] |
TopDownProteomicsi | P78346-1 [P78346-1] |
Protocols and materials databases
Antibodypediai | 16338, 81 antibodies |
DNASUi | 10556 |
Genome annotation databases
Ensembli | ENST00000371703; ENSP00000360768; ENSG00000148688 [P78346-1] ENST00000413330; ENSP00000389182; ENSG00000148688 [P78346-2] |
GeneIDi | 10556 |
KEGGi | hsa:10556 |
UCSCi | uc009xtx.4, human [P78346-1] |
Organism-specific databases
CTDi | 10556 |
GeneCardsi | RPP30 |
HGNCi | HGNC:17688, RPP30 |
HPAi | ENSG00000148688, Low tissue specificity |
MIMi | 606115, gene |
neXtProti | NX_P78346 |
OpenTargetsi | ENSG00000148688 |
PharmGKBi | PA134876345 |
VEuPathDBi | HostDB:ENSG00000148688.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2363, Eukaryota |
GeneTreei | ENSGT00390000000883 |
InParanoidi | P78346 |
OMAi | PWDVINL |
OrthoDBi | 1197518at2759 |
PhylomeDBi | P78346 |
TreeFami | TF106113 |
Enzyme and pathway databases
PathwayCommonsi | P78346 |
Reactomei | R-HSA-6784531, tRNA processing in the nucleus R-HSA-6791226, Major pathway of rRNA processing in the nucleolus and cytosol |
Miscellaneous databases
BioGRID-ORCSi | 10556, 612 hits in 995 CRISPR screens |
ChiTaRSi | RPP30, human |
GeneWikii | RPP30 |
GenomeRNAii | 10556 |
Pharosi | P78346, Tbio |
PROi | PR:P78346 |
RNActi | P78346, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000148688, Expressed in testis and 239 other tissues |
ExpressionAtlasi | P78346, baseline and differential |
Genevisiblei | P78346, HS |
Family and domain databases
InterProi | View protein in InterPro IPR016195, Pol/histidinol_Pase-like IPR002738, RNase_P_p30 |
PANTHERi | PTHR13031, PTHR13031, 1 hit |
Pfami | View protein in Pfam PF01876, RNase_P_p30, 1 hit |
SUPFAMi | SSF89550, SSF89550, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RPP30_HUMAN | |
Accessioni | P78346Primary (citable) accession number: P78346 Secondary accession number(s): B2R799, E9PB02 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | May 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 187 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families