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Protein

Eukaryotic translation initiation factor 4 gamma 2

Gene

EIF4G2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.4 Publications

Miscellaneous

This gene has been shown to be extensively edited in the liver of APOBEC1 transgenic animal model. Its aberrant editing could contribute to the potent oncogenesis induced by overexpression of APOBEC1. The aberrant edited sequence, called NAT1, is likely to be a fundamental translational repressor.

Caution

According to PubMed:9049310, this sequence initiates exclusively at a GTG codon.Curated

GO - Molecular functioni

  • cadherin binding Source: BHF-UCL
  • mRNA binding Source: GO_Central
  • RNA binding Source: UniProtKB
  • translation factor activity, RNA binding Source: UniProtKB
  • translation initiation factor activity Source: UniProtKB

GO - Biological processi

  • cell cycle arrest Source: ProtInc
  • cell death Source: ProtInc
  • cellular macromolecule biosynthetic process Source: ParkinsonsUK-UCL
  • negative regulation of autophagy Source: ParkinsonsUK-UCL
  • positive regulation of cell growth Source: ParkinsonsUK-UCL
  • regulation of translational initiation Source: UniProtKB

Keywordsi

Molecular functionInitiation factor, Repressor
Biological processProtein biosynthesis, Translation regulation

Enzyme and pathway databases

ReactomeiR-HSA-1169408 ISG15 antiviral mechanism

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 4 gamma 2
Short name:
eIF-4-gamma 2
Short name:
eIF-4G 2
Short name:
eIF4G 2
Alternative name(s):
Death-associated protein 5
Short name:
DAP-5
p97
Gene namesi
Name:EIF4G2Imported
Synonyms:DAP51 Publication
ORF Names:OK/SW-cl.75
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000110321.15
HGNCiHGNC:3297 EIF4G2
MIMi602325 gene
neXtProtiNX_P78344

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi1982
OpenTargetsiENSG00000110321
PharmGKBiPA27723

Polymorphism and mutation databases

BioMutaiEIF4G2
DMDMi30315906

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002133251 – 907Eukaryotic translation initiation factor 4 gamma 2Add BLAST907

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1 Publication1
Modified residuei11PhosphoserineCombined sources1
Modified residuei89PhosphothreonineCombined sources1
Modified residuei360Omega-N-methylarginineCombined sources1
Modified residuei395PhosphoserineCombined sources1
Modified residuei431N6-methyllysineCombined sources1
Modified residuei443PhosphoserineCombined sources1
Modified residuei505Omega-N-methylarginineCombined sources1
Modified residuei508PhosphothreonineCombined sources1
Modified residuei514PhosphothreonineCombined sources1
Cross-linki575Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei902PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation; hyperphosphorylated during mitosis.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP78344
MaxQBiP78344
PaxDbiP78344
PeptideAtlasiP78344
PRIDEiP78344
ProteomicsDBi57574
57575 [P78344-2]

PTM databases

iPTMnetiP78344
PhosphoSitePlusiP78344
SwissPalmiP78344

Miscellaneous databases

PMAP-CutDBiP78344

Expressioni

Tissue specificityi

Ubiquitously expressed in all adult tissues examined, with high levels in skeletal muscle and heart. Also expressed in fetal brain, lung, liver and kidney.3 Publications

Gene expression databases

BgeeiENSG00000110321 Expressed in 242 organ(s), highest expression level in visceral pleura
CleanExiHS_EIF4G2
ExpressionAtlasiP78344 baseline and differential
GenevisibleiP78344 HS

Organism-specific databases

HPAiCAB005163
HPA006773
HPA016965

Interactioni

Subunit structurei

Interacts with the serine/threonine protein kinases MKNK1 and MKNK2. Binds EIF4A and EIF3. Interacts with MIF4GD.4 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi108297, 76 interactors
DIPiDIP-31366N
IntActiP78344, 26 interactors
MINTiP78344
STRINGi9606.ENSP00000340281

Structurei

Secondary structure

1907
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP78344
SMRiP78344
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP78344

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini78 – 308MIF4GPROSITE-ProRule annotationAdd BLAST231
Domaini543 – 666MIPROSITE-ProRule annotationAdd BLAST124
Domaini720 – 904W2PROSITE-ProRule annotationAdd BLAST185

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IPIB Eukaryota
ENOG410ZIZB LUCA
GeneTreeiENSGT00530000063038
HOVERGENiHBG052084
InParanoidiP78344
KOiK03260
OrthoDBiEOG091G03ZW
PhylomeDBiP78344

Family and domain databases

Gene3Di1.25.40.180, 3 hits
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR003891 Initiation_fac_eIF4g_MI
IPR016021 MIF4-like_sf
IPR003890 MIF4G-like_typ-3
IPR003307 W2_domain
PfamiView protein in Pfam
PF02847 MA3, 1 hit
PF02854 MIF4G, 1 hit
PF02020 W2, 1 hit
SMARTiView protein in SMART
SM00515 eIF5C, 1 hit
SM00544 MA3, 1 hit
SM00543 MIF4G, 1 hit
SUPFAMiSSF48371 SSF48371, 3 hits
PROSITEiView protein in PROSITE
PS51366 MI, 1 hit
PS51363 W2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 15 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: P78344-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MESAIAEGGA SRFSASSGGG GSRGAPQHYP KTAGNSEFLG KTPGQNAQKW
60 70 80 90 100
IPARSTRRDD NSAANNSANE KERHDAIFRK VRGILNKLTP EKFDKLCLEL
110 120 130 140 150
LNVGVESKLI LKGVILLIVD KALEEPKYSS LYAQLCLRLA EDAPNFDGPA
160 170 180 190 200
AEGQPGQKQS TTFRRLLISK LQDEFENRTR NVDVYDKREN PLLPEEEEQR
210 220 230 240 250
AIAKIKMLGN IKFIGELGKL DLIHESILHK CIKTLLEKKK RVQLKDMGED
260 270 280 290 300
LECLCQIMRT VGPRLDHERA KSLMDQYFAR MCSLMLSKEL PARIRFLLQD
310 320 330 340 350
TVELREHHWV PRKAFLDNGP KTINQIRQDA VKDLGVFIPA PMAQGMRSDF
360 370 380 390 400
FLEGPFMPPR MKMDRDPLGG LADMFGQMPG SGIGTGPGVI QDRFSPTMGR
410 420 430 440 450
HRSNQLFNGH GGHIMPPTQS QFGEMGGKFM KSQGLSQLYH NQSQGLLSQL
460 470 480 490 500
QGQSKDMPPR FSKKGQLNAD EISLRPAQSF LMNKNQVPKL QPQITMIPPS
510 520 530 540 550
AQPPRTQTPP LGQTPQLGLK TNPPLIQEKP AKTSKKPPPS KEELLKLTET
560 570 580 590 600
VVTEYLNSGN ANEAVNGVRE MRAPKHFLPE MLSKVIILSL DRSDEDKEKA
610 620 630 640 650
SSLISLLKQE GIATSDNFMQ AFLNVLDQCP KLEVDIPLVK SYLAQFAARA
660 670 680 690 700
IISELVSISE LAQPLESGTH FPLFLLCLQQ LAKLQDREWL TELFQQSKVN
710 720 730 740 750
MQKMLPEIDQ NKDRMLEILE GKGLSFLFPL LKLEKELLKQ IKLDPSPQTI
760 770 780 790 800
YKWIKDNISP KLHVDKGFVN ILMTSFLQYI SSEVNPPSDE TDSSSAPSKE
810 820 830 840 850
QLEQEKQLLL SFKPVMQKFL HDHVDLQVSA LYALQVHCYN SNFPKGMLLR
860 870 880 890 900
FFVHFYDMEI IEEEAFLAWK EDITQEFPGK GKALFQVNQW LTWLETAEEE

ESEEEAD
Length:907
Mass (Da):102,362
Last modified:May 1, 1997 - v1
Checksum:i4EF050B5EEA4DF91
GO
Isoform 2 (identifier: P78344-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     434-471: Missing.

Note: No experimental confirmation available.
Show »
Length:869
Mass (Da):98,150
Checksum:iF400E1E28654A2E4
GO

Computationally mapped potential isoform sequencesi

There are 15 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3DQV9D3DQV9_HUMAN
Eukaryotic translation initiation f...
EIF4G2 hCG_1991788
907Annotation score:
H0Y3P2H0Y3P2_HUMAN
Eukaryotic translation initiation f...
EIF4G2
869Annotation score:
H0YCH5H0YCH5_HUMAN
Eukaryotic translation initiation f...
EIF4G2
444Annotation score:
E9PKF8E9PKF8_HUMAN
Eukaryotic translation initiation f...
EIF4G2
185Annotation score:
H0YEC5H0YEC5_HUMAN
Eukaryotic translation initiation f...
EIF4G2
244Annotation score:
H0YD77H0YD77_HUMAN
Eukaryotic translation initiation f...
EIF4G2
282Annotation score:
H0YE44H0YE44_HUMAN
Eukaryotic translation initiation f...
EIF4G2
88Annotation score:
H0YDC0H0YDC0_HUMAN
Eukaryotic translation initiation f...
EIF4G2
116Annotation score:
H0YCF8H0YCF8_HUMAN
Eukaryotic translation initiation f...
EIF4G2
129Annotation score:
H0YE22H0YE22_HUMAN
Eukaryotic translation initiation f...
EIF4G2
234Annotation score:
There are more potential isoformsShow all

Sequence cautioni

The sequence BAB93515 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAD97268 differs from that shown. Unusual initiator. The initiator methionine is coded by a non-canonical GTG valine codon.Curated
The sequence CAA61857 differs from that shown. Unusual initiator. The initiator methionine is coded by a non-canonical GTG valine codon.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti169S → P in AAC51166 (PubMed:9030685).Curated1
Sequence conflicti233K → Q in AAC51166 (PubMed:9030685).Curated1
Sequence conflicti245K → Q in AAC51166 (PubMed:9030685).Curated1
Sequence conflicti351F → L in AAC51166 (PubMed:9030685).Curated1
Sequence conflicti441N → S in AAH18746 (PubMed:15489334).Curated1
Sequence conflicti531A → G in AAC51166 (PubMed:9030685).Curated1
Sequence conflicti534S → N in AAH43149 (PubMed:15489334).Curated1
Sequence conflicti613A → G in AAC51166 (PubMed:9030685).Curated1
Sequence conflicti861I → S in AAC51166 (PubMed:9030685).Curated1
Sequence conflicti899E → G in AAC51166 (PubMed:9030685).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_048923236L → M. Corresponds to variant dbSNP:rs34885591Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_038726434 – 471Missing in isoform 2. 1 PublicationAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73824 mRNA Translation: AAB49973.1
U76111 mRNA Translation: AAC51166.1
X89713 mRNA Translation: CAA61857.1 Sequence problems.
AK223548 mRNA Translation: BAD97268.1 Sequence problems.
AC116535 Genomic DNA No translation available.
BC014930 mRNA Translation: AAH14930.2
BC018746 mRNA Translation: AAH18746.1
BC018975 mRNA Translation: AAH18975.1
BC039851 mRNA Translation: AAH39851.1
BC043149 mRNA Translation: AAH43149.2
BC111415 mRNA Translation: AAI11416.1
BC111548 mRNA Translation: AAI11549.2
AB063323 mRNA Translation: BAB93515.1 Different initiation.
CCDSiCCDS31428.1 [P78344-1]
CCDS41618.1 [P78344-2]
RefSeqiNP_001036024.3, NM_001042559.2 [P78344-2]
NP_001166176.1, NM_001172705.1 [P78344-1]
NP_001409.3, NM_001418.3 [P78344-1]
UniGeneiHs.183684

Genome annotation databases

EnsembliENST00000640650; ENSP00000492820; ENSG00000110321 [P78344-1]
GeneIDi1982
KEGGihsa:1982
UCSCiuc057zbi.1 human [P78344-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73824 mRNA Translation: AAB49973.1
U76111 mRNA Translation: AAC51166.1
X89713 mRNA Translation: CAA61857.1 Sequence problems.
AK223548 mRNA Translation: BAD97268.1 Sequence problems.
AC116535 Genomic DNA No translation available.
BC014930 mRNA Translation: AAH14930.2
BC018746 mRNA Translation: AAH18746.1
BC018975 mRNA Translation: AAH18975.1
BC039851 mRNA Translation: AAH39851.1
BC043149 mRNA Translation: AAH43149.2
BC111415 mRNA Translation: AAI11416.1
BC111548 mRNA Translation: AAI11549.2
AB063323 mRNA Translation: BAB93515.1 Different initiation.
CCDSiCCDS31428.1 [P78344-1]
CCDS41618.1 [P78344-2]
RefSeqiNP_001036024.3, NM_001042559.2 [P78344-2]
NP_001166176.1, NM_001172705.1 [P78344-1]
NP_001409.3, NM_001418.3 [P78344-1]
UniGeneiHs.183684

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3D3MX-ray1.90A/B730-897[»]
3L6AX-ray2.00A540-897[»]
4IULX-ray2.30A/B61-323[»]
ProteinModelPortaliP78344
SMRiP78344
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108297, 76 interactors
DIPiDIP-31366N
IntActiP78344, 26 interactors
MINTiP78344
STRINGi9606.ENSP00000340281

PTM databases

iPTMnetiP78344
PhosphoSitePlusiP78344
SwissPalmiP78344

Polymorphism and mutation databases

BioMutaiEIF4G2
DMDMi30315906

Proteomic databases

EPDiP78344
MaxQBiP78344
PaxDbiP78344
PeptideAtlasiP78344
PRIDEiP78344
ProteomicsDBi57574
57575 [P78344-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000640650; ENSP00000492820; ENSG00000110321 [P78344-1]
GeneIDi1982
KEGGihsa:1982
UCSCiuc057zbi.1 human [P78344-1]

Organism-specific databases

CTDi1982
DisGeNETi1982
EuPathDBiHostDB:ENSG00000110321.15
GeneCardsiEIF4G2
HGNCiHGNC:3297 EIF4G2
HPAiCAB005163
HPA006773
HPA016965
MIMi602325 gene
neXtProtiNX_P78344
OpenTargetsiENSG00000110321
PharmGKBiPA27723
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IPIB Eukaryota
ENOG410ZIZB LUCA
GeneTreeiENSGT00530000063038
HOVERGENiHBG052084
InParanoidiP78344
KOiK03260
OrthoDBiEOG091G03ZW
PhylomeDBiP78344

Enzyme and pathway databases

ReactomeiR-HSA-1169408 ISG15 antiviral mechanism

Miscellaneous databases

ChiTaRSiEIF4G2 human
EvolutionaryTraceiP78344
GeneWikiiEIF4G2
GenomeRNAii1982
PMAP-CutDBiP78344
PROiPR:P78344
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000110321 Expressed in 242 organ(s), highest expression level in visceral pleura
CleanExiHS_EIF4G2
ExpressionAtlasiP78344 baseline and differential
GenevisibleiP78344 HS

Family and domain databases

Gene3Di1.25.40.180, 3 hits
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR003891 Initiation_fac_eIF4g_MI
IPR016021 MIF4-like_sf
IPR003890 MIF4G-like_typ-3
IPR003307 W2_domain
PfamiView protein in Pfam
PF02847 MA3, 1 hit
PF02854 MIF4G, 1 hit
PF02020 W2, 1 hit
SMARTiView protein in SMART
SM00515 eIF5C, 1 hit
SM00544 MA3, 1 hit
SM00543 MIF4G, 1 hit
SUPFAMiSSF48371 SSF48371, 3 hits
PROSITEiView protein in PROSITE
PS51366 MI, 1 hit
PS51363 W2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIF4G2_HUMAN
AccessioniPrimary (citable) accession number: P78344
Secondary accession number(s): O60877
, P78404, Q0VH00, Q0VH01, Q2NKW9, Q49A79, Q53EU1, Q58EZ2, Q8NI71, Q96C16
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: May 1, 1997
Last modified: November 7, 2018
This is version 179 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
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