UniProtKB - P78061 (PUUA_ECOLI)
Gamma-glutamylputrescine synthetase PuuA
puuA
Functioni
Involved in the breakdown of putrescine. Catalyzes the ATP-dependent gamma-glutamylation of putrescine, producing gamma-L-glutamylputrescine. Absolutely essential to utilize putrescine as both nitrogen and carbon sources and to decrease the toxicity of putrescine, which can lead to inhibition of cell growth and protein synthesis. In vitro is also able to use several diamines, and spermidine and spermine, instead of putrescine, but with a much lower activity, and cannot catalyze the gamma-glutamylation of ornithine or GABA (PubMed:18495664).
2 PublicationsCatalytic activityi
Cofactori
Kineticsi
- KM=2.07 mM for glutamate (at pH 8 and at 37 degrees Celsius)1 Publication
- KM=2.25 mM for ATP (at pH 8 and at 37 degrees Celsius)1 Publication
- KM=44.6 mM for putrescine (at pH 8 and at 37 degrees Celsius)1 Publication
- Vmax=6.71 µmol/min/mg enzyme with putrescine as substrate (at pH 8 and at 37 degrees Celsius)1 Publication
pH dependencei
: putrescine degradation Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 4-aminobutanoate from putrescine.1 Publication This subpathway is part of the pathway putrescine degradation, which is itself part of Amine and polyamine degradation.View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-aminobutanoate from putrescine, the pathway putrescine degradation and in Amine and polyamine degradation.
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- glutamate-ammonia ligase activity Source: InterPro
- glutamate-putrescine ligase activity Source: EcoCyc
GO - Biological processi
- glutamine biosynthetic process Source: GO_Central
- polyamine catabolic process Source: GO_Central
- putrescine catabolic process Source: EcoCyc
Keywordsi
Molecular function | Ligase |
Ligand | ATP-binding, Magnesium, Manganese, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:G6644-MONOMER |
UniPathwayi | UPA00188;UER00880 |
Names & Taxonomyi
Protein namesi | Recommended name: Gamma-glutamylputrescine synthetase PuuA1 Publication (EC:6.3.1.111 Publication)Short name: Gamma-Glu-Put synthetase Alternative name(s): Glutamate--putrescine ligase1 Publication |
Gene namesi | Name:puuA1 Publication Synonyms:ycjK Ordered Locus Names:b1297, JW5201 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
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Pathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 282 | H → N: Activity is impaired to 9% of wild-type. 1 Publication | 1 | |
Mutagenesisi | 357 | R → Q: Activity is impaired to 3% of wild-type. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000153280 | 1 – 472 | Gamma-glutamylputrescine synthetase PuuAAdd BLAST | 472 |
Proteomic databases
jPOSTi | P78061 |
PaxDbi | P78061 |
PRIDEi | P78061 |
Expressioni
Inductioni
Interactioni
Subunit structurei
Dodecamer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 4260944, 13 interactors |
STRINGi | 511145.b1297 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 35 – 129 | GS beta-graspPROSITE-ProRule annotationAdd BLAST | 95 | |
Domaini | 136 – 472 | GS catalyticPROSITE-ProRule annotationAdd BLAST | 337 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0174, Bacteria |
HOGENOMi | CLU_017290_0_0_6 |
InParanoidi | P78061 |
OMAi | NPGQHEI |
PhylomeDBi | P78061 |
Family and domain databases
Gene3Di | 3.10.20.70, 1 hit |
InterProi | View protein in InterPro IPR036651, Gln_synt_N IPR014746, Gln_synth/guanido_kin_cat_dom IPR008146, Gln_synth_cat_dom IPR027303, Gln_synth_gly_rich_site |
Pfami | View protein in Pfam PF00120, Gln-synt_C, 1 hit |
SMARTi | View protein in SMART SM01230, Gln-synt_C, 1 hit |
SUPFAMi | SSF54368, SSF54368, 1 hit SSF55931, SSF55931, 1 hit |
PROSITEi | View protein in PROSITE PS00181, GLNA_ATP, 1 hit PS51986, GS_BETA_GRASP, 1 hit PS51987, GS_CATALYTIC, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
METNIVEVEN FVQQSEERRG SAFTQEVKRY LERYPNTQYV DVLLTDLNGC
60 70 80 90 100
FRGKRIPVSS LKKLEKGCYF PASVFAMDIL GNVVEEAGLG QEMGEPDRTC
110 120 130 140 150
VPVLGSLTPS AADPEFIGQM LLTMVDEDGA PFDVEPRNVL NRLWQQLRQR
160 170 180 190 200
GLFPVVAVEL EFYLLDRQRD AEGYLQPPCA PGTDDRNTQS QVYSVDNLNH
210 220 230 240 250
FADVLNDIDE LAQLQLIPAD GAVAEASPGQ FEINLYHTDN VLEACDDALA
260 270 280 290 300
LKRLVRLMAE KHKMHATFMA KPYEEHAGSG MHIHISMQNN RGENVLSDAE
310 320 330 340 350
GEDSPLLKKM LAGMIDLMPS SMALLAPNVN SYRRFQPGMY VPTQASWGHN
360 370 380 390 400
NRTVALRIPC GDRHNHRVEY RVAGADANPY LVMAAIFAGI LHGLDNELPL
410 420 430 440 450
QEEVEGNGLE QEGLPFPIRQ SDALGEFIEN DHLRRYLGER FCHVYHACKN
460 470
DELLQFERLI TETEIEWMLK NA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74379.2 AP009048 Genomic DNA Translation: BAA14857.2 |
PIRi | D64878 |
RefSeqi | NP_415813.4, NC_000913.3 WP_001296746.1, NZ_STEB01000005.1 |
Genome annotation databases
EnsemblBacteriai | AAC74379; AAC74379; b1297 BAA14857; BAA14857; BAA14857 |
GeneIDi | 66674875 946202 |
KEGGi | ecj:JW5201 eco:b1297 |
PATRICi | fig|511145.12.peg.1352 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74379.2 AP009048 Genomic DNA Translation: BAA14857.2 |
PIRi | D64878 |
RefSeqi | NP_415813.4, NC_000913.3 WP_001296746.1, NZ_STEB01000005.1 |
3D structure databases
AlphaFoldDBi | P78061 |
SMRi | P78061 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4260944, 13 interactors |
STRINGi | 511145.b1297 |
Proteomic databases
jPOSTi | P78061 |
PaxDbi | P78061 |
PRIDEi | P78061 |
Genome annotation databases
EnsemblBacteriai | AAC74379; AAC74379; b1297 BAA14857; BAA14857; BAA14857 |
GeneIDi | 66674875 946202 |
KEGGi | ecj:JW5201 eco:b1297 |
PATRICi | fig|511145.12.peg.1352 |
Organism-specific databases
EchoBASEi | EB3667 |
Phylogenomic databases
eggNOGi | COG0174, Bacteria |
HOGENOMi | CLU_017290_0_0_6 |
InParanoidi | P78061 |
OMAi | NPGQHEI |
PhylomeDBi | P78061 |
Enzyme and pathway databases
UniPathwayi | UPA00188;UER00880 |
BioCyci | EcoCyc:G6644-MONOMER |
Miscellaneous databases
PROi | PR:P78061 |
Family and domain databases
Gene3Di | 3.10.20.70, 1 hit |
InterProi | View protein in InterPro IPR036651, Gln_synt_N IPR014746, Gln_synth/guanido_kin_cat_dom IPR008146, Gln_synth_cat_dom IPR027303, Gln_synth_gly_rich_site |
Pfami | View protein in Pfam PF00120, Gln-synt_C, 1 hit |
SMARTi | View protein in SMART SM01230, Gln-synt_C, 1 hit |
SUPFAMi | SSF54368, SSF54368, 1 hit SSF55931, SSF55931, 1 hit |
PROSITEi | View protein in PROSITE PS00181, GLNA_ATP, 1 hit PS51986, GS_BETA_GRASP, 1 hit PS51987, GS_CATALYTIC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PUUA_ECOLI | |
Accessioni | P78061Primary (citable) accession number: P78061 Secondary accession number(s): P78287 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | July 11, 2001 | |
Last modified: | May 25, 2022 | |
This is version 149 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families