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Protein

Pyruvate kinase

Gene

pyk

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Activity regulationi

Regulated by phosphoenolpyruvate substrate and is allosterically activated by ribose-5-phosphate, AMP and other nucleoside monophosphates but not by fructose-1,6-bisphosphate.By similarity

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gapA)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)
  4. Enolase (eno)
  5. Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei56SubstrateBy similarity1
Metal bindingi58PotassiumBy similarity1
Metal bindingi60PotassiumBy similarity1
Metal bindingi90PotassiumBy similarity1
Metal bindingi91Potassium; via carbonyl oxygenBy similarity1
Sitei249Transition state stabilizerBy similarity1
Metal bindingi251MagnesiumBy similarity1
Binding sitei274Substrate; via amide nitrogenBy similarity1
Metal bindingi275MagnesiumBy similarity1
Binding sitei275Substrate; via amide nitrogenBy similarity1
Binding sitei307SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • plasminogen activation Source: AgBase

Keywordsi

Molecular functionAllosteric enzyme, Kinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

BioCyciMPNE272634:G1GJ3-473-MONOMER
UniPathwayi
UPA00109;UER00188

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pyk
Ordered Locus Names:MPN_303
ORF Names:MP533
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000808 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001120811 – 508Pyruvate kinaseAdd BLAST508

Proteomic databases

PRIDEiP78031

Interactioni

Subunit structurei

Homotetramer.By similarity

Binary interactionsi

Protein-protein interaction databases

IntActiP78031, 5 interactors

Structurei

3D structure databases

ProteinModelPortaliP78031
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

KOiK00873
OMAiKHEAIEQ

Family and domain databases

Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 1 hit
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

P78031-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIHHLKRTKI IATCGPALTK KLWTLAMLDD PAYAAMKAEA YANIENIIKN
60 70 80 90 100
GVTVIRLNFS HGNHEEQAVR IKIVRDVAKK LNLPVSIMLD TNGPEIRVFE
110 120 130 140 150
TAPEGLKILK DSEVVINTTT KEVAKNNQFS VSDASGTYNM VNDVKVGQKI
160 170 180 190 200
LVDDGKLSLV VKRIDTKNNQ VICVAQNDHT IFTKKRLNLP NADYSIPFLS
210 220 230 240 250
AKDLRDIDFG LTHQIDYIAA SFVNTTENIK QLRDYLASKN AKHVKLIAKI
260 270 280 290 300
ESNHALNNID GIIKASDGIM VARGDLGLEI PYYKVPYWQR YMIKACRFFN
310 320 330 340 350
KRVITATQML DSLEKNIQPT RAEVTDVYFA VDRGNDATML SGETANGAFP
360 370 380 390 400
LNAVYVMKMI DKQSETFFDY QYNLNYYMAN SKARHSEFWK QVVLPLAQKT
410 420 430 440 450
APKRKLINSD FKYDFVVHAT NNLNEIYALS NARLAAAVII LTNDPQVYTG
460 470 480 490 500
HGVDYGIFPY LIDQKPQSLS KAEFKSLANV AIKHYQQHGE ISQLKQCLGV

FHNKIISL
Length:508
Mass (Da):57,268
Last modified:February 1, 1997 - v1
Checksum:i011D97377F8BB527
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA Translation: AAB96181.1
PIRiS73859
RefSeqiNP_109991.1, NC_000912.1
WP_010874660.1, NC_000912.1

Genome annotation databases

EnsemblBacteriaiAAB96181; AAB96181; MPN_303
GeneIDi877217
KEGGimpn:MPN303
PATRICifig|272634.6.peg.327

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA Translation: AAB96181.1
PIRiS73859
RefSeqiNP_109991.1, NC_000912.1
WP_010874660.1, NC_000912.1

3D structure databases

ProteinModelPortaliP78031
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP78031, 5 interactors

Proteomic databases

PRIDEiP78031

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB96181; AAB96181; MPN_303
GeneIDi877217
KEGGimpn:MPN303
PATRICifig|272634.6.peg.327

Phylogenomic databases

KOiK00873
OMAiKHEAIEQ

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00188

BioCyciMPNE272634:G1GJ3-473-MONOMER

Family and domain databases

Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 1 hit
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKPYK_MYCPN
AccessioniPrimary (citable) accession number: P78031
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: September 12, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
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Main funding by: National Institutes of Health

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