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Protein

Dihydrofolate reductase

Gene

folA

Organism
Mycoplasma pneumoniae (strain ATCC 29342 / M129)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Dihydrofolate reductase (folA)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei27SubstrateBy similarity1
Binding sitei32SubstrateBy similarity1
Binding sitei57SubstrateBy similarity1
Binding sitei120SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi6 – 7NADPBy similarity2
Nucleotide bindingi14 – 19NADPBy similarity6
Nucleotide bindingi43 – 46NADPBy similarity4
Nucleotide bindingi62 – 65NADPBy similarity4
Nucleotide bindingi101 – 106NADPBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processOne-carbon metabolism
LigandNADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-579
MPNE272634:G1GJ3-512-MONOMER
UniPathwayi
UPA00077;UER00158

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase (EC:1.5.1.3)
Gene namesi
Name:folA
Synonyms:dhfR
Ordered Locus Names:MPN_321
ORF Names:MP515
OrganismiMycoplasma pneumoniae (strain ATCC 29342 / M129)
Taxonomic identifieri272634 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000808 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863961 – 160Dihydrofolate reductaseAdd BLAST160

Interactioni

Protein-protein interaction databases

IntActiP78028, 5 interactors

Structurei

3D structure databases

ProteinModelPortaliP78028
SMRiP78028
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 160DHFRPROSITE-ProRule annotationAdd BLAST160

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni5 – 7Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated

Phylogenomic databases

KOiK00287
OMAiEECFVIG

Family and domain databases

CDDicd00209 DHFR, 1 hit
Gene3Di3.40.430.10, 1 hit
InterProiView protein in InterPro
IPR012259 DHFR
IPR024072 DHFR-like_dom_sf
IPR017925 DHFR_CS
IPR001796 DHFR_dom
PANTHERiPTHR22778:SF16 PTHR22778:SF16, 1 hit
PfamiView protein in Pfam
PF00186 DHFR_1, 1 hit
SUPFAMiSSF53597 SSF53597, 1 hit
PROSITEiView protein in PROSITE
PS00075 DHFR_1, 1 hit
PS51330 DHFR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P78028-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVKAIWAMDQ NGLIGNGNSL PWRIKAELQH FRQTTLHQDV LMGSATYLSL
60 70 80 90 100
PPVFSERNVY ILTRNLNFNP PDKGCLTKVI HEYENFIQPY LHHPDKHLYI
110 120 130 140 150
CGGAQVYEQL IPRCDALIVS TIFGKYTGDK YLKVDFSPFE LTKEISFAEF
160
KVAYYHKIAR
Length:160
Mass (Da):18,511
Last modified:February 1, 1997 - v1
Checksum:i87C323433A3FABA4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA Translation: AAB96163.1
PIRiS73841
RefSeqiNP_110009.1, NC_000912.1
WP_010874677.1, NC_000912.1

Genome annotation databases

EnsemblBacteriaiAAB96163; AAB96163; MPN_321
GeneIDi876943
KEGGimpn:MPN321
PATRICifig|272634.6.peg.345

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00089 Genomic DNA Translation: AAB96163.1
PIRiS73841
RefSeqiNP_110009.1, NC_000912.1
WP_010874677.1, NC_000912.1

3D structure databases

ProteinModelPortaliP78028
SMRiP78028
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP78028, 5 interactors

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB96163; AAB96163; MPN_321
GeneIDi876943
KEGGimpn:MPN321
PATRICifig|272634.6.peg.345

Phylogenomic databases

KOiK00287
OMAiEECFVIG

Enzyme and pathway databases

UniPathwayi
UPA00077;UER00158

BioCyciMetaCyc:MONOMER-579
MPNE272634:G1GJ3-512-MONOMER

Family and domain databases

CDDicd00209 DHFR, 1 hit
Gene3Di3.40.430.10, 1 hit
InterProiView protein in InterPro
IPR012259 DHFR
IPR024072 DHFR-like_dom_sf
IPR017925 DHFR_CS
IPR001796 DHFR_dom
PANTHERiPTHR22778:SF16 PTHR22778:SF16, 1 hit
PfamiView protein in Pfam
PF00186 DHFR_1, 1 hit
SUPFAMiSSF53597 SSF53597, 1 hit
PROSITEiView protein in PROSITE
PS00075 DHFR_1, 1 hit
PS51330 DHFR_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDYR_MYCPN
AccessioniPrimary (citable) accession number: P78028
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Mycoplasma pneumoniae
    Mycoplasma pneumoniae (strain M129): entries and gene names
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Main funding by: National Institutes of Health

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