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Protein

Carbamoyl-phosphate synthase pyrimidine-specific small chain

Gene

pyrAA

Organism
Lactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.

Enzyme regulationi

Inhibited by pyrimidine.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes (S)-dihydroorotate from bicarbonate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Carbamoyl-phosphate synthase pyrimidine-specific small chain (pyrAA), Carbamoyl-phosphate synthase pyrimidine-specific large chain (pyrAB), Carbamoyl-phosphate synthase (glutamine-hydrolyzing) (pyrAB2), Carbamoyl-phosphate synthase small chain (pyrAA2)
  2. Aspartate carbamoyltransferase (pyrB)
  3. Dihydroorotase (pyrC)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-dihydroorotate from bicarbonate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei244NucleophileBy similarity1
Active sitei329By similarity1
Active sitei331By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPyrimidine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLPLA220668:G1GW0-2313-MONOMER
UniPathwayiUPA00070; UER00115

Protein family/group databases

MEROPSiC26.963

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase pyrimidine-specific small chain (EC:6.3.5.5)
Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chain
Short name:
CPS-P
Gene namesi
Name:pyrAA
Ordered Locus Names:lp_2701
OrganismiLactobacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
Taxonomic identifieri220668 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
Proteomesi
  • UP000000432 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001122861 – 364Carbamoyl-phosphate synthase pyrimidine-specific small chainAdd BLAST364

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate.

Protein-protein interaction databases

STRINGi220668.lp_2701

Structurei

3D structure databases

ProteinModelPortaliP77885
SMRiP77885
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini169 – 356Glutamine amidotransferase type-1Add BLAST188

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 165CPSaseAdd BLAST165

Sequence similaritiesi

Belongs to the CarA family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105C1M Bacteria
COG0505 LUCA
HOGENOMiHOG000038087
KOiK01956
OMAiCFNTGMT

Family and domain databases

CDDicd01744 GATase1_CPSase, 1 hit
Gene3Di3.40.50.880, 1 hit
3.50.30.20, 1 hit
HAMAPiMF_01209 CPSase_S_chain, 1 hit
InterProiView protein in InterPro
IPR006274 CarbamoylP_synth_ssu
IPR002474 CarbamoylP_synth_ssu_N
IPR036480 CarbP_synth_ssu_N_sf
IPR029062 Class_I_gatase-like
IPR035686 CPSase_GATase1
IPR017926 GATASE
PfamiView protein in Pfam
PF00988 CPSase_sm_chain, 1 hit
PF00117 GATase, 1 hit
SMARTiView protein in SMART
SM01097 CPSase_sm_chain, 1 hit
SUPFAMiSSF52021 SSF52021, 1 hit
SSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR01368 CPSaseIIsmall, 1 hit
PROSITEiView protein in PROSITE
PS51273 GATASE_TYPE_1, 1 hit

Sequencei

Sequence statusi: Complete.

P77885-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRYLVLEDG TIYPGTGFGA TTATVGELVF NTGMSGYQES ITDQSYNGEI
60 70 80 90 100
LMFTYPLIGN YGINRDDHES IKPTCKGVVV HEVARRASNW RNAQSLDDYL
110 120 130 140 150
KQNAIPGIMD IDTRAVTKHI RTKGAMKATI VDNVLPDTVD RLKVTELNRA
160 170 180 190 200
VVAQSSTNNA YPNPATGPNV VVVDFGLKHS ILRELAKRQC NLTVLPYNTT
210 220 230 240 250
ASEIMALNPD GVMLTNGPGD PKDVPGALEM IREVEKHVPL FGICLGHQLF
260 270 280 290 300
ALANGADTFK MKFGHRGFNH PVREIATGRI DFTSQNHGYA VDRDSLAQTD
310 320 330 340 350
LLITHEEIND GTVEGLRHRD YAAFSVQYHP DAAPGPHDAD HIFDEFIDLM
360
AANQATQKGS QFNA
Length:364
Mass (Da):39,983
Last modified:March 25, 2003 - v2
Checksum:i5B916580EE4C3267
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti144V → I in CAA91004 (PubMed:8982065).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54240 Genomic DNA Translation: CAA91004.1
AL935263 Genomic DNA Translation: CCC79822.1
RefSeqiWP_011101887.1, NC_004567.2
YP_004890336.1, NC_004567.2

Genome annotation databases

EnsemblBacteriaiCCC79822; CCC79822; lp_2701
GeneIDi1062973
KEGGilpl:lp_2701
PATRICifig|220668.9.peg.2261

Similar proteinsi

Entry informationi

Entry nameiCARA_LACPL
AccessioniPrimary (citable) accession number: P77885
Secondary accession number(s): F9URI3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: March 25, 2003
Last modified: February 28, 2018
This is version 117 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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