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UniProtKB - P77791 (MAA_ECOLI)
Protein
Maltose O-acetyltransferase
Gene
maa
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the CoA-dependent transfer of an acetyl group to maltose and other sugars (PubMed:1856235).
Acetylates glucose exclusively at the C6 position and maltose at the C6 position of the non-reducing end glucosyl moiety. Is able to acetylate maltooligosaccharides (PubMed:12731863).
2 PublicationsCatalytic activityi
- EC:2.3.1.792 Publications
Kineticsi
Acetylates glucose, maltose, mannose, galactose, and fructose with a decreasing relative rate of 1, 0.55, 0.20, 0.07, 0.04.1 Publication
- KM=62 mM for glucose1 Publication
- KM=90 mM for maltose1 Publication
- KM=71 mM for glucose1 Publication
- KM=42.9 mM for maltose1 Publication
- KM=285.5 mM for maltotriose1 Publication
- Vmax=0.20 mmol/min/mg enzyme with glucose as substrate1 Publication
- Vmax=0.11 mmol/min/mg enzyme with maltose as substrate1 Publication
pH dependencei
Optimum pH is 7.8.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 83 | Acetyl-CoABy similarity | 1 | |
Sitei | 83 | Transition state stabilizerBy similarity | 1 | |
Active sitei | 113 | Proton donor/acceptorBy similarity | 1 | |
Binding sitei | 140 | Acetyl-CoA; via amide nitrogenBy similarity | 1 | |
Binding sitei | 158 | Acetyl-CoA; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Binding sitei | 178 | Acetyl-CoA; shared with dimeric partnerBy similarity | 1 | |
Binding sitei | 181 | Acetyl-CoABy similarity | 1 |
GO - Molecular functioni
- identical protein binding Source: EcoCyc
- maltose O-acetyltransferase activity Source: EcoCyc
- O-acyltransferase activity Source: GO_Central
Keywordsi
Molecular function | Acyltransferase, Transferase |
Enzyme and pathway databases
BioCyci | EcoCyc:MALTACETYLTRAN-MONOMER |
BRENDAi | 2.3.1.79, 2026 |
Names & Taxonomyi
Protein namesi | Recommended name: Maltose O-acetyltransferase1 Publication (EC:2.3.1.792 Publications)Short name: MAT1 Publication Alternative name(s): Maltose transacetylase1 Publication |
Gene namesi | Name:maa Synonyms:ylaD Ordered Locus Names:b0459, JW0448 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytosol
- cytosol Source: GO_Central
Other locations
- protein-containing complex Source: EcoCyc
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000068729 | 2 – 183 | Maltose O-acetyltransferaseAdd BLAST | 182 |
Proteomic databases
jPOSTi | P77791 |
PaxDbi | P77791 |
PRIDEi | P77791 |
2D gel databases
SWISS-2DPAGEi | P77791 |
Interactioni
Subunit structurei
Homodimer.
1 PublicationGO - Molecular functioni
- identical protein binding Source: EcoCyc
Protein-protein interaction databases
BioGRIDi | 4261202, 4 interactors |
DIPi | DIP-10140N |
IntActi | P77791, 16 interactors |
STRINGi | 511145.b0459 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P77791 |
SMRi | P77791 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P77791 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 163 – 164 | Acetyl-CoA binding; shared with dimeric partnerBy similarity | 2 |
Sequence similaritiesi
Belongs to the transferase hexapeptide repeat family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | COG0110, Bacteria |
HOGENOMi | CLU_051638_3_3_6 |
InParanoidi | P77791 |
OMAi | DCQIGPN |
PhylomeDBi | P77791 |
Family and domain databases
InterProi | View protein in InterPro IPR001451, Hexapep IPR018357, Hexapep_transf_CS IPR024688, Mac_dom IPR011004, Trimer_LpxA-like_sf |
Pfami | View protein in Pfam PF14602, Hexapep_2, 2 hits PF12464, Mac, 1 hit |
SMARTi | View protein in SMART SM01266, Mac, 1 hit |
SUPFAMi | SSF51161, SSF51161, 1 hit |
PROSITEi | View protein in PROSITE PS00101, HEXAPEP_TRANSFERASES, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P77791-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTEKEKMIA GELYRSADET LSRDRLRARQ LIHRYNHSLA EEHTLRQQIL
60 70 80 90 100
ADLFGQVTEA YIEPTFRCDY GYNIFLGNNF FANFDCVMLD VCPIRIGDNC
110 120 130 140 150
MLAPGVHIYT ATHPIDPVAR NSGAELGKPV TIGNNVWIGG RAVINPGVTI
160 170 180
GDNVVVASGA VVTKDVPDNV VVGGNPARII KKL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ223173 Genomic DNA Translation: CAA11147.1 U82664 Genomic DNA Translation: AAB40214.1 U00096 Genomic DNA Translation: AAC73561.1 AP009048 Genomic DNA Translation: BAE76238.1 |
PIRi | B64776 |
RefSeqi | NP_414992.1, NC_000913.3 WP_000102564.1, NZ_SSUW01000008.1 |
Genome annotation databases
EnsemblBacteriai | AAC73561; AAC73561; b0459 BAE76238; BAE76238; BAE76238 |
GeneIDi | 58460091 945096 |
KEGGi | ecj:JW0448 eco:b0459 |
PATRICi | fig|1411691.4.peg.1817 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ223173 Genomic DNA Translation: CAA11147.1 U82664 Genomic DNA Translation: AAB40214.1 U00096 Genomic DNA Translation: AAC73561.1 AP009048 Genomic DNA Translation: BAE76238.1 |
PIRi | B64776 |
RefSeqi | NP_414992.1, NC_000913.3 WP_000102564.1, NZ_SSUW01000008.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1OCX | X-ray | 2.15 | A/B/C | 2-183 | [»] | |
6AG8 | X-ray | 1.34 | A/C | 1-183 | [»] | |
AlphaFoldDBi | P77791 | |||||
SMRi | P77791 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4261202, 4 interactors |
DIPi | DIP-10140N |
IntActi | P77791, 16 interactors |
STRINGi | 511145.b0459 |
2D gel databases
SWISS-2DPAGEi | P77791 |
Proteomic databases
jPOSTi | P77791 |
PaxDbi | P77791 |
PRIDEi | P77791 |
Genome annotation databases
EnsemblBacteriai | AAC73561; AAC73561; b0459 BAE76238; BAE76238; BAE76238 |
GeneIDi | 58460091 945096 |
KEGGi | ecj:JW0448 eco:b0459 |
PATRICi | fig|1411691.4.peg.1817 |
Organism-specific databases
EchoBASEi | EB3990 |
Phylogenomic databases
eggNOGi | COG0110, Bacteria |
HOGENOMi | CLU_051638_3_3_6 |
InParanoidi | P77791 |
OMAi | DCQIGPN |
PhylomeDBi | P77791 |
Enzyme and pathway databases
BioCyci | EcoCyc:MALTACETYLTRAN-MONOMER |
BRENDAi | 2.3.1.79, 2026 |
Miscellaneous databases
EvolutionaryTracei | P77791 |
PROi | PR:P77791 |
Family and domain databases
InterProi | View protein in InterPro IPR001451, Hexapep IPR018357, Hexapep_transf_CS IPR024688, Mac_dom IPR011004, Trimer_LpxA-like_sf |
Pfami | View protein in Pfam PF14602, Hexapep_2, 2 hits PF12464, Mac, 1 hit |
SMARTi | View protein in SMART SM01266, Mac, 1 hit |
SUPFAMi | SSF51161, SSF51161, 1 hit |
PROSITEi | View protein in PROSITE PS00101, HEXAPEP_TRANSFERASES, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MAA_ECOLI | |
Accessioni | P77791Primary (citable) accession number: P77791 Secondary accession number(s): Q2MBW8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 154 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families