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UniProtKB - P77783 (YNFF_ECOLI)
Protein
Probable dimethyl sulfoxide reductase chain YnfF
Gene
ynfF
Organism
Escherichia coli (strain K12)
Status
Functioni
Terminal reductase during anaerobic growth on various sulfoxide and N-oxide compounds.
By similarityCofactori
Protein has several cofactor binding sites:- [4Fe-4S] clusterCuratedNote: Binds 1 [4Fe-4S] cluster.Curated
- Mo-bis(molybdopterin guanine dinucleotide)By similarityNote: Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 59 | Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 63 | Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 67 | Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 99 | Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation | 1 | |
Metal bindingi | 195 | Molybdenum (Mo-bis(molybdopterin guanine dinucleotide) metal ligand)By similarity | 1 |
GO - Molecular functioni
- 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
- dimethyl sulfoxide reductase activity Source: InterPro
- electron transfer activity Source: GO_Central
- molybdenum ion binding Source: GO_Central
- molybdopterin cofactor binding Source: InterPro
- selenate reductase activity Source: EcoCyc
GO - Biological processi
- anaerobic respiration Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase |
Ligand | 4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum |
Enzyme and pathway databases
BioCyci | EcoCyc:G6846-MONOMER |
Protein family/group databases
TCDBi | 5.A.3.3.1, the prokaryotic molybdopterin-containing oxidoreductase (pmo) family |
Names & Taxonomyi
Protein namesi | Recommended name: Probable dimethyl sulfoxide reductase chain YnfF (EC:1.8.99.-)Short name: DMSO reductase |
Gene namesi | Name:ynfF Ordered Locus Names:b1588, JW5260 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- outer membrane-bounded periplasmic space Source: GO_Central
- oxidoreductase complex Source: ComplexPortal
Keywords - Cellular componenti
Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 45 | Tat-type signalPROSITE-ProRule annotationAdd BLAST | 45 | |
ChainiPRO_0000019148 | 46 – 807 | Probable dimethyl sulfoxide reductase chain YnfFAdd BLAST | 762 |
Post-translational modificationi
Exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. Can also be exported by the Sec system.
Proteomic databases
jPOSTi | P77783 |
PaxDbi | P77783 |
PRIDEi | P77783 |
Interactioni
Subunit structurei
The complex consists of three subunits: YnfF, the reductase; YnfG, an electron transfer protein, and YnfH, a membrane anchor protein.
CuratedBinary interactionsi
P77783
With | #Exp. | IntAct |
---|---|---|
dmsD [P69853] | 3 | EBI-6406285,EBI-4406374 |
Protein-protein interaction databases
BioGRIDi | 4259130, 9 interactors |
ComplexPortali | CPX-6019, Putative dimethyl sulfoxide reductase |
DIPi | DIP-12766N |
IntActi | P77783, 2 interactors |
STRINGi | 511145.b1588 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 52 – 113 | 4Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST | 62 |
Sequence similaritiesi
Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG0243, Bacteria |
HOGENOMi | CLU_000422_13_3_6 |
InParanoidi | P77783 |
OMAi | NELMPYS |
PhylomeDBi | P77783 |
Family and domain databases
InterProi | View protein in InterPro IPR011888, Anaer_DMSO_reductase IPR009010, Asp_de-COase-like_dom_sf IPR006657, MoPterin_dinucl-bd_dom IPR006656, Mopterin_OxRdtase IPR006963, Mopterin_OxRdtase_4Fe-4S_dom IPR006655, Mopterin_OxRdtase_prok_CS IPR027467, MopterinOxRdtase_cofactor_BS IPR006311, TAT_signal |
Pfami | View protein in Pfam PF04879, Molybdop_Fe4S4, 1 hit PF00384, Molybdopterin, 1 hit PF01568, Molydop_binding, 1 hit |
SMARTi | View protein in SMART SM00926, Molybdop_Fe4S4, 1 hit |
SUPFAMi | SSF50692, SSF50692, 1 hit |
TIGRFAMsi | TIGR02166, dmsA_ynfE, 1 hit |
PROSITEi | View protein in PROSITE PS51669, 4FE4S_MOW_BIS_MGD, 1 hit PS00551, MOLYBDOPTERIN_PROK_1, 1 hit PS00932, MOLYBDOPTERIN_PROK_3, 1 hit PS51318, TAT, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P77783-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKIHTTEALM KAEISRRSLM KTSALGSLAL ASSAFTLPFS QMVRAAEAPV
60 70 80 90 100
EEKAVWSSCT VNCGSRCLLR LHVKDDTVYW VESDTTGDDV YGNHQVRACL
110 120 130 140 150
RGRSIRRRMN HPDRLKYPMK RVGKRGEGKF ERISWDEALD TISDNLRRIL
160 170 180 190 200
KDYGNEAVHV LYGTGVDGGN ITNSNVPYRL MNSCGGFLSR YGSYSTAQIS
210 220 230 240 250
AAMSYMFGAN DGNSPDDIAN TKLVVMFGNN PAETRMSGGG VTYYVEQARE
260 270 280 290 300
RSNARMIVID PRYNDTAAGR EDEWLPIRPG TDGALACAIA WVLITENMVD
310 320 330 340 350
QPFLDKYCVG YDEKTLPANA PRNAHYKAYI LGEGPDGIAK TPEWAAKITS
360 370 380 390 400
IPAEKIIQLA REIGSAKPAY ICQGWGPQRH SNGEQTSRAI AMLSVLTGNV
410 420 430 440 450
GINGGNSGVR EGSWDLGVEW FPMLENPVKT QISVFTWTDA IDHGTEMTAT
460 470 480 490 500
RDGVRGKEKL DVPIKFLWCY ASNTLINQHG DINHTHEVLQ DDSKCEMIVG
510 520 530 540 550
IDHFMTASAK YCDILLPDLM PTEQEDLISH ESAGNMGYVI LAQPATSAKF
560 570 580 590 600
ERKPIYWMLS EVAKRLGPDV YQTFTEGRSQ HEWIKYLHAK TKERNPEMPD
610 620 630 640 650
YEEMKTTGIF KKKCPEEHYV AFRAFREDPQ ANPLKTPSGK IEIYSERLAK
660 670 680 690 700
IADTWELKKD EIIHPLPAYT PGFDGWDDPL RKTYPLQLTG FHYKARTHSS
710 720 730 740 750
YGNIDVLQQA CPQEVWINPI DAQARGIRHG DTVRVFNNNG EMLIAAKVTP
760 770 780 790 800
RILPGVTAIG QGAWLKADMF GDRVDHGGSI NILTSHRPSP LAKGNPSHSN
LVQIEKV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74660.4 AP009048 Genomic DNA Translation: BAA15312.2 |
PIRi | F64914 |
RefSeqi | NP_416105.4, NC_000913.3 WP_001340362.1, NZ_SSZK01000001.1 |
Genome annotation databases
EnsemblBacteriai | AAC74660; AAC74660; b1588 BAA15312; BAA15312; BAA15312 |
GeneIDi | 66674522 945268 |
KEGGi | ecj:JW5260 eco:b1588 |
PATRICi | fig|511145.12.peg.1659 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74660.4 AP009048 Genomic DNA Translation: BAA15312.2 |
PIRi | F64914 |
RefSeqi | NP_416105.4, NC_000913.3 WP_001340362.1, NZ_SSZK01000001.1 |
3D structure databases
AlphaFoldDBi | P77783 |
SMRi | P77783 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259130, 9 interactors |
ComplexPortali | CPX-6019, Putative dimethyl sulfoxide reductase |
DIPi | DIP-12766N |
IntActi | P77783, 2 interactors |
STRINGi | 511145.b1588 |
Protein family/group databases
TCDBi | 5.A.3.3.1, the prokaryotic molybdopterin-containing oxidoreductase (pmo) family |
Proteomic databases
jPOSTi | P77783 |
PaxDbi | P77783 |
PRIDEi | P77783 |
Genome annotation databases
EnsemblBacteriai | AAC74660; AAC74660; b1588 BAA15312; BAA15312; BAA15312 |
GeneIDi | 66674522 945268 |
KEGGi | ecj:JW5260 eco:b1588 |
PATRICi | fig|511145.12.peg.1659 |
Organism-specific databases
EchoBASEi | EB3605 |
Phylogenomic databases
eggNOGi | COG0243, Bacteria |
HOGENOMi | CLU_000422_13_3_6 |
InParanoidi | P77783 |
OMAi | NELMPYS |
PhylomeDBi | P77783 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6846-MONOMER |
Miscellaneous databases
PROi | PR:P77783 |
Family and domain databases
InterProi | View protein in InterPro IPR011888, Anaer_DMSO_reductase IPR009010, Asp_de-COase-like_dom_sf IPR006657, MoPterin_dinucl-bd_dom IPR006656, Mopterin_OxRdtase IPR006963, Mopterin_OxRdtase_4Fe-4S_dom IPR006655, Mopterin_OxRdtase_prok_CS IPR027467, MopterinOxRdtase_cofactor_BS IPR006311, TAT_signal |
Pfami | View protein in Pfam PF04879, Molybdop_Fe4S4, 1 hit PF00384, Molybdopterin, 1 hit PF01568, Molydop_binding, 1 hit |
SMARTi | View protein in SMART SM00926, Molybdop_Fe4S4, 1 hit |
SUPFAMi | SSF50692, SSF50692, 1 hit |
TIGRFAMsi | TIGR02166, dmsA_ynfE, 1 hit |
PROSITEi | View protein in PROSITE PS51669, 4FE4S_MOW_BIS_MGD, 1 hit PS00551, MOLYBDOPTERIN_PROK_1, 1 hit PS00932, MOLYBDOPTERIN_PROK_3, 1 hit PS51318, TAT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | YNFF_ECOLI | |
Accessioni | P77783Primary (citable) accession number: P77783 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | July 22, 2008 | |
Last modified: | May 25, 2022 | |
This is version 179 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families