UniProtKB - P77774 (BAMB_ECOLI)
Protein
Outer membrane protein assembly factor BamB
Gene
bamB
Organism
Escherichia coli (strain K12)
Status
Functioni
Part of the outer membrane protein assembly complex (Bam), which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Nonessential member of the complex, which may orient the flexible periplasmic domain of BamA for interaction with other Bam components, chaperones and nascent outer membrane proteins. Efficient substrate folding and insertion into the outer membrane requires all 5 subunits (PubMed:20378773, PubMed:21823654, PubMed:27686148). A lateral gate may open between the first and last strands of the BamA beta-barrel that allows substrate to insert into the outer membrane; comparison of the structures of complete and nearly complete Bam complexes show there is considerable movement of all 5 proteins (PubMed:27686148, PubMed:26900875, PubMed:26901871).7 Publications
GO - Molecular functioni
- identical protein binding Source: IntAct
GO - Biological processi
- Gram-negative-bacterium-type cell outer membrane assembly Source: EcoCyc
- protein insertion into membrane Source: EcoCyc
Enzyme and pathway databases
BioCyci | EcoCyc:G7320-MONOMER |
Protein family/group databases
TCDBi | 1.B.33.1.3, the outer membrane protein insertion porin (bam complex) (ompip) family |
Names & Taxonomyi
Protein namesi | Recommended name: Outer membrane protein assembly factor BamBUniRule annotation |
Gene namesi | Name:bamBUniRule annotation Synonyms:yfgL Ordered Locus Names:b2512, JW2496 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell outer membrane UniRule annotation3 Publications; Lipid-anchor UniRule annotation2 Publications
GO - Cellular componenti
- Bam protein complex Source: EcoCyc
- cell outer membrane Source: EcoCyc
Keywords - Cellular componenti
Cell outer membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 19 | UniRule annotationAdd BLAST | 19 | |
ChainiPRO_0000042211 | 20 – 392 | Outer membrane protein assembly factor BamBAdd BLAST | 373 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 20 | N-palmitoyl cysteine1 Publication | 1 | |
Lipidationi | 20 | S-diacylglycerol cysteine1 Publication | 1 |
Keywords - PTMi
Lipoprotein, PalmitateProteomic databases
jPOSTi | P77774 |
PaxDbi | P77774 |
PRIDEi | P77774 |
Interactioni
Subunit structurei
Part of the Bam complex, which is composed of the outer membrane protein BamA, and four lipoproteins BamB, BamC, BamD and BamE. Monomer.
Interacts directly with BamA. The Bam complex has the shape of a hat, with the BamA beta-barrel crown in the outer membrane and the periplasmic brim formed by the BamA POTRA domains and the 4 lipoproteins (PubMed:27686148, PubMed:26900875, PubMed:26901871).
9 PublicationsBinary interactionsi
Hide detailsP77774
With | #Exp. | IntAct |
---|---|---|
bamA [P0A940] | 23 | EBI-907297,EBI-907371 |
itself | 2 | EBI-907297,EBI-907297 |
surA [P0ABZ6] | 2 | EBI-907297,EBI-558651 |
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 4260587, 321 interactors |
ComplexPortali | CPX-1923, BAM complex |
DIPi | DIP-12042N |
IntActi | P77774, 9 interactors |
STRINGi | 511145.b2512 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P77774 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P77774 |
Family & Domainsi
Sequence similaritiesi
Belongs to the BamB family.UniRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | COG1520, Bacteria |
HOGENOMi | CLU_027480_0_1_6 |
InParanoidi | P77774 |
PhylomeDBi | P77774 |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
HAMAPi | MF_00923, OM_assembly_BamB, 1 hit |
InterProi | View protein in InterPro IPR017687, BamB IPR018391, PQQ_beta_propeller_repeat IPR002372, PQQ_repeat IPR011047, Quinoprotein_ADH-like_supfam IPR015943, WD40/YVTN_repeat-like_dom_sf |
Pfami | View protein in Pfam PF13360, PQQ_2, 1 hit |
SMARTi | View protein in SMART SM00564, PQQ, 7 hits |
SUPFAMi | SSF50998, SSF50998, 1 hit |
TIGRFAMsi | TIGR03300, assembly_YfgL, 1 hit |
PROSITEi | View protein in PROSITE PS51257, PROKAR_LIPOPROTEIN, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P77774-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQLRKLLLPG LLSVTLLSGC SLFNSEEDVV KMSPLPTVEN QFTPTTAWST
60 70 80 90 100
SVGSGIGNFY SNLHPALADN VVYAADRAGL VKALNADDGK EIWSVSLAEK
110 120 130 140 150
DGWFSKEPAL LSGGVTVSGG HVYIGSEKAQ VYALNTSDGT VAWQTKVAGE
160 170 180 190 200
ALSRPVVSDG LVLIHTSNGQ LQALNEADGA VKWTVNLDMP SLSLRGESAP
210 220 230 240 250
TTAFGAAVVG GDNGRVSAVL MEQGQMIWQQ RISQATGSTE IDRLSDVDTT
260 270 280 290 300
PVVVNGVVFA LAYNGNLTAL DLRSGQIMWK RELGSVNDFI VDGNRIYLVD
310 320 330 340 350
QNDRVMALTI DGGVTLWTQS DLLHRLLTSP VLYNGNLVVG DSEGYLHWIN
360 370 380 390
VEDGRFVAQQ KVDSSGFQTE PVAADGKLLI QAKDGTVYSI TR
Mass spectrometryi
Molecular mass is 40643 Da. Determined by ESI. With 3 palmitic acid (C16) acyl chains.1 Publication
Molecular mass is 40663 Da. Determined by ESI. With 2 palmitic (C16) and 1 stearic (C18) acid acyl chains.1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75565.1 AP009048 Genomic DNA Translation: BAA16398.1 |
PIRi | G65027 |
RefSeqi | NP_417007.1, NC_000913.3 WP_001177052.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75565; AAC75565; b2512 BAA16398; BAA16398; BAA16398 |
GeneIDi | 946982 |
KEGGi | ecj:JW2496 eco:b2512 |
PATRICi | fig|1411691.4.peg.4224 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75565.1 AP009048 Genomic DNA Translation: BAA16398.1 |
PIRi | G65027 |
RefSeqi | NP_417007.1, NC_000913.3 WP_001177052.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2YH3 | X-ray | 2.60 | A | 22-392 | [»] | |
2YMS | X-ray | 2.10 | A | 62-191 | [»] | |
B | 113-186 | [»] | ||||
C | 248-322 | [»] | ||||
D | 247-320 | [»] | ||||
3P1L | X-ray | 2.60 | A | 21-392 | [»] | |
3PRW | X-ray | 1.80 | A | 21-392 | [»] | |
3Q7M | X-ray | 1.65 | A | 21-392 | [»] | |
3Q7N | X-ray | 1.77 | A | 21-392 | [»] | |
3Q7O | X-ray | 2.09 | A | 21-392 | [»] | |
4PK1 | X-ray | 3.10 | A | 21-392 | [»] | |
4XGA | X-ray | 2.15 | A | 20-392 | [»] | |
5AYW | X-ray | 3.56 | B | 20-392 | [»] | |
5D0O | X-ray | 2.90 | B | 1-392 | [»] | |
5LJO | electron microscopy | 4.90 | B | 22-392 | [»] | |
6SN9 | electron microscopy | 9.80 | B | 22-392 | [»] | |
6V05 | electron microscopy | 4.10 | B | 1-392 | [»] | |
SMRi | P77774 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260587, 321 interactors |
ComplexPortali | CPX-1923, BAM complex |
DIPi | DIP-12042N |
IntActi | P77774, 9 interactors |
STRINGi | 511145.b2512 |
Protein family/group databases
TCDBi | 1.B.33.1.3, the outer membrane protein insertion porin (bam complex) (ompip) family |
Proteomic databases
jPOSTi | P77774 |
PaxDbi | P77774 |
PRIDEi | P77774 |
Genome annotation databases
EnsemblBacteriai | AAC75565; AAC75565; b2512 BAA16398; BAA16398; BAA16398 |
GeneIDi | 946982 |
KEGGi | ecj:JW2496 eco:b2512 |
PATRICi | fig|1411691.4.peg.4224 |
Organism-specific databases
EchoBASEi | EB3960 |
Phylogenomic databases
eggNOGi | COG1520, Bacteria |
HOGENOMi | CLU_027480_0_1_6 |
InParanoidi | P77774 |
PhylomeDBi | P77774 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7320-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P77774 |
PROi | PR:P77774 |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
HAMAPi | MF_00923, OM_assembly_BamB, 1 hit |
InterProi | View protein in InterPro IPR017687, BamB IPR018391, PQQ_beta_propeller_repeat IPR002372, PQQ_repeat IPR011047, Quinoprotein_ADH-like_supfam IPR015943, WD40/YVTN_repeat-like_dom_sf |
Pfami | View protein in Pfam PF13360, PQQ_2, 1 hit |
SMARTi | View protein in SMART SM00564, PQQ, 7 hits |
SUPFAMi | SSF50998, SSF50998, 1 hit |
TIGRFAMsi | TIGR03300, assembly_YfgL, 1 hit |
PROSITEi | View protein in PROSITE PS51257, PROKAR_LIPOPROTEIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | BAMB_ECOLI | |
Accessioni | P77774Primary (citable) accession number: P77774 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | February 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 164 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families