UniProtKB - P77757 (ARNC_ECOLI)
Protein
Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase
Gene
arnC
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the transfer of 4-deoxy-4-formamido-L-arabinose from UDP to undecaprenyl phosphate. The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides.1 Publication
Catalytic activityi
- di-trans,octa-cis-undecaprenyl phosphate + UDP-4-deoxy-4-formamido-β-L-arabinose = 4-deoxy-4-formamido-α-L-arabinopyranosyl di-trans,octa-cis-undecaprenyl phosphate + UDP1 PublicationEC:2.4.2.531 Publication
: 4-amino-4-deoxy-alpha-L-arabinose undecaprenyl phosphate biosynthesis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 4-amino-4-deoxy-alpha-L-arabinose undecaprenyl phosphate from UDP-4-deoxy-4-formamido-beta-L-arabinose and undecaprenyl phosphate.1 PublicationProteins known to be involved in the 2 steps of the subpathway in this organism are:
- Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase (arnC), Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase (arnC)
- Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD (arnD), Probable 4-deoxy-4-formamido-L-arabinose-phosphoundecaprenol deformylase ArnD (arnD)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-amino-4-deoxy-alpha-L-arabinose undecaprenyl phosphate from UDP-4-deoxy-4-formamido-beta-L-arabinose and undecaprenyl phosphate, the pathway 4-amino-4-deoxy-alpha-L-arabinose undecaprenyl phosphate biosynthesis and in Glycolipid biosynthesis.
Pathwayi: lipopolysaccharide biosynthesis
This protein is involved in the pathway lipopolysaccharide biosynthesis, which is part of Bacterial outer membrane biogenesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway lipopolysaccharide biosynthesis and in Bacterial outer membrane biogenesis.
GO - Molecular functioni
- phosphotransferase activity, for other substituted phosphate groups Source: UniProtKB-UniRule
- undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase activity Source: EcoCyc
GO - Biological processi
- 4-amino-4-deoxy-alpha-L-arabinopyranosyl undecaprenyl phosphate biosynthetic process Source: UniProtKB-UniPathway
- lipid A biosynthetic process Source: UniProtKB-UniRule
- lipopolysaccharide biosynthetic process Source: UniProtKB-UniPathway
- response to antibiotic Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Antibiotic resistance, Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism, Lipopolysaccharide biosynthesis |
Enzyme and pathway databases
BioCyci | EcoCyc:G7167-MONOMER MetaCyc:G7167-MONOMER |
UniPathwayi | UPA00030 UPA00036;UER00495 |
Protein family/group databases
CAZyi | GT2, Glycosyltransferase Family 2 |
TCDBi | 4.D.1.1.13, the putative vectorial glycosyl polymerization (vgp) family |
Names & Taxonomyi
Protein namesi | Recommended name: Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferase (EC:2.4.2.53)Alternative name(s): Polymyxin resistance protein PmrF Undecaprenyl-phosphate Ara4FN transferase Short name: Ara4FN transferase |
Gene namesi | Name:arnC Synonyms:pmrF, yfbF Ordered Locus Names:b2254, JW2248 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Plasma membrane
Plasma Membrane
- plasma membrane Source: EcoCyc
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane Source: EcoCyc
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 235 | CytoplasmicSequence analysisAdd BLAST | 235 | |
Transmembranei | 236 – 256 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 257 – 269 | PeriplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 270 – 290 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 291 – 322 | CytoplasmicSequence analysisAdd BLAST | 32 |
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000059195 | 1 – 322 | Undecaprenyl-phosphate 4-deoxy-4-formamido-L-arabinose transferaseAdd BLAST | 322 |
Proteomic databases
jPOSTi | P77757 |
PaxDbi | P77757 |
PRIDEi | P77757 |
Expressioni
Inductioni
Induced by BasR.By similarity
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260496, 315 interactors |
IntActi | P77757, 3 interactors |
STRINGi | 511145.b2254 |
Family & Domainsi
Sequence similaritiesi
Belongs to the glycosyltransferase 2 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG0463, Bacteria |
HOGENOMi | CLU_033536_0_0_6 |
InParanoidi | P77757 |
PhylomeDBi | P77757 |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
HAMAPi | MF_01164, ArnC_transfer, 1 hit |
InterProi | View protein in InterPro IPR022857, ArnC_tfrase IPR001173, Glyco_trans_2-like IPR029044, Nucleotide-diphossugar_trans |
Pfami | View protein in Pfam PF00535, Glycos_transf_2, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
i Sequence
Sequence statusi: Complete.
P77757-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFEIHPVKKV SVVIPVYNEQ ESLPELIRRT TTACESLGKE YEILLIDDGS
60 70 80 90 100
SDNSAHMLVE ASQAENSHIV SILLNRNYGQ HSAIMAGFSH VTGDLIITLD
110 120 130 140 150
ADLQNPPEEI PRLVAKADEG YDVVGTVRQN RQDSWFRKTA SKMINRLIQR
160 170 180 190 200
TTGKAMGDYG CMLRAYRRHI VDAMLHCHER STFIPILANI FARRAIEIPV
210 220 230 240 250
HHAEREFGES KYSFMRLINL MYDLVTCLTT TPLRMLSLLG SIIAIGGFSI
260 270 280 290 300
AVLLVILRLT FGPQWAAEGV FMLFAVLFTF IGAQFIGMGL LGEYIGRIYT
310 320
DVRARPRYFV QQVIRPSSKE NE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ011863 Genomic DNA Translation: AAY34350.1 U00096 Genomic DNA Translation: AAC75314.1 AP009048 Genomic DNA Translation: BAA16077.1 |
PIRi | D64996 |
RefSeqi | NP_416757.1, NC_000913.3 WP_000461657.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75314; AAC75314; b2254 BAA16077; BAA16077; BAA16077 |
GeneIDi | 57727993 945275 |
KEGGi | ecj:JW2248 eco:b2254 |
PATRICi | fig|1411691.4.peg.4483 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ011863 Genomic DNA Translation: AAY34350.1 U00096 Genomic DNA Translation: AAC75314.1 AP009048 Genomic DNA Translation: BAA16077.1 |
PIRi | D64996 |
RefSeqi | NP_416757.1, NC_000913.3 WP_000461657.1, NZ_LN832404.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4260496, 315 interactors |
IntActi | P77757, 3 interactors |
STRINGi | 511145.b2254 |
Protein family/group databases
CAZyi | GT2, Glycosyltransferase Family 2 |
TCDBi | 4.D.1.1.13, the putative vectorial glycosyl polymerization (vgp) family |
Proteomic databases
jPOSTi | P77757 |
PaxDbi | P77757 |
PRIDEi | P77757 |
Genome annotation databases
EnsemblBacteriai | AAC75314; AAC75314; b2254 BAA16077; BAA16077; BAA16077 |
GeneIDi | 57727993 945275 |
KEGGi | ecj:JW2248 eco:b2254 |
PATRICi | fig|1411691.4.peg.4483 |
Organism-specific databases
EchoBASEi | EB3843 |
Phylogenomic databases
eggNOGi | COG0463, Bacteria |
HOGENOMi | CLU_033536_0_0_6 |
InParanoidi | P77757 |
PhylomeDBi | P77757 |
Enzyme and pathway databases
UniPathwayi | UPA00030 UPA00036;UER00495 |
BioCyci | EcoCyc:G7167-MONOMER MetaCyc:G7167-MONOMER |
Miscellaneous databases
PROi | PR:P77757 |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
HAMAPi | MF_01164, ArnC_transfer, 1 hit |
InterProi | View protein in InterPro IPR022857, ArnC_tfrase IPR001173, Glyco_trans_2-like IPR029044, Nucleotide-diphossugar_trans |
Pfami | View protein in Pfam PF00535, Glycos_transf_2, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ARNC_ECOLI | |
Accessioni | P77757Primary (citable) accession number: P77757 Secondary accession number(s): P78179, Q4U4N1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | February 1, 1997 | |
Last modified: | February 10, 2021 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families