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Protein

1-deoxyxylulose-5-phosphate synthase YajO

Gene

yajO

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of ribulose 5-phosphate (Ru5P) to 1-deoxy-D-xylulose 5-phosphate (DXP), providing a direct route from pentoses to terpenes. May play a role in biosynthesis of DXP under conditions of thiamine starvation.1 Publication

Miscellaneous

Overexpression supports cell viability in the absence of the essential dxs-encoded 1-deoxy-D-xylulose-5-phosphate synthase (PubMed:25326299). Overexpression confers resistance to the 4-amino-2-methyl 5-hydroxymethylpyrimidine (HMP) analogs bacimethrin and 4-amino-2-trifluoromethyl 5-hydroxymethylpyrimidine (CF3-HMP) (PubMed:15292217).2 Publications

Catalytic activityi

AH2 + D-ribulose 5-phosphate = 1-deoxy-D-xylulose 5-phosphate + A + H2O.1 Publication

Activity regulationi

NADH, NADPH or ATP do not increase activity.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei61Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase

Enzyme and pathway databases

BioCyciEcoCyc:G6236-MONOMER
MetaCyc:G6236-MONOMER

Protein family/group databases

TCDBi8.A.5.1.6 the voltage-gated k(+) channel -subunit (kv) family

Names & Taxonomyi

Protein namesi
Recommended name:
1-deoxyxylulose-5-phosphate synthase YajOCurated (EC:1.1.-.-1 Publication)
Gene namesi
Name:yajO
Ordered Locus Names:b0419, JW0409
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13611 yajO

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000703921 – 3241-deoxyxylulose-5-phosphate synthase YajOAdd BLAST324

Proteomic databases

PaxDbiP77735
PRIDEiP77735

Interactioni

Protein-protein interaction databases

BioGridi4259835, 111 interactors
DIPiDIP-11291N
IntActiP77735, 6 interactors
STRINGi316385.ECDH10B_0375

Structurei

3D structure databases

ProteinModelPortaliP77735
SMRiP77735
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CPC Bacteria
COG0667 LUCA
HOGENOMiHOG000250270
InParanoidiP77735
PhylomeDBiP77735

Family and domain databases

CDDicd06660 Aldo_ket_red, 1 hit
Gene3Di3.20.20.100, 1 hit
InterProiView protein in InterPro
IPR020471 Aldo/keto_reductase
IPR023210 NADP_OxRdtase_dom
IPR036812 NADP_OxRdtase_dom_sf
PfamiView protein in Pfam
PF00248 Aldo_ket_red, 1 hit
PRINTSiPR00069 ALDKETRDTASE
SUPFAMiSSF51430 SSF51430, 1 hit

Sequencei

Sequence statusi: Complete.

P77735-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQYNPLGKTD LRVSRLCLGC MTFGEPDRGN HAWTLPEESS RPIIKRALEG
60 70 80 90 100
GINFFDTANS YSDGSSEEIV GRALRDFARR EDVVVATKVF HRVGDLPEGL
110 120 130 140 150
SRAQILRSID DSLRRLGMDY VDILQIHRWD YNTPIEETLE ALNDVVKAGK
160 170 180 190 200
ARYIGASSMH ASQFAQALEL QKQHGWAQFV SMQDHYNLIY REEEREMLPL
210 220 230 240 250
CYQEGVAVIP WSPLARGRLT RPWGETTARL VSDEVGKNLY KESDENDAQI
260 270 280 290 300
AERLTGVSEE LGATRAQVAL AWLLSKPGIA APIIGTSREE QLDELLNAVD
310 320
ITLKPEQIAE LETPYKPHPV VGFK
Length:324
Mass (Da):36,420
Last modified:December 15, 1998 - v2
Checksum:i0B69E09F2BEDF9B1
GO

Sequence cautioni

The sequence AAB40175 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82664 Genomic DNA Translation: AAB40175.1 Different initiation.
U00096 Genomic DNA Translation: AAC73522.2
AP009048 Genomic DNA Translation: BAE76199.1
PIRiC64771
RefSeqiNP_414953.2, NC_000913.3
WP_001199800.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73522; AAC73522; b0419
BAE76199; BAE76199; BAE76199
GeneIDi946903
KEGGiecj:JW0409
eco:b0419
PATRICifig|1411691.4.peg.1858

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82664 Genomic DNA Translation: AAB40175.1 Different initiation.
U00096 Genomic DNA Translation: AAC73522.2
AP009048 Genomic DNA Translation: BAE76199.1
PIRiC64771
RefSeqiNP_414953.2, NC_000913.3
WP_001199800.1, NZ_LN832404.1

3D structure databases

ProteinModelPortaliP77735
SMRiP77735
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259835, 111 interactors
DIPiDIP-11291N
IntActiP77735, 6 interactors
STRINGi316385.ECDH10B_0375

Protein family/group databases

TCDBi8.A.5.1.6 the voltage-gated k(+) channel -subunit (kv) family

Proteomic databases

PaxDbiP77735
PRIDEiP77735

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73522; AAC73522; b0419
BAE76199; BAE76199; BAE76199
GeneIDi946903
KEGGiecj:JW0409
eco:b0419
PATRICifig|1411691.4.peg.1858

Organism-specific databases

EchoBASEiEB3377
EcoGeneiEG13611 yajO

Phylogenomic databases

eggNOGiENOG4105CPC Bacteria
COG0667 LUCA
HOGENOMiHOG000250270
InParanoidiP77735
PhylomeDBiP77735

Enzyme and pathway databases

BioCyciEcoCyc:G6236-MONOMER
MetaCyc:G6236-MONOMER

Miscellaneous databases

PROiPR:P77735

Family and domain databases

CDDicd06660 Aldo_ket_red, 1 hit
Gene3Di3.20.20.100, 1 hit
InterProiView protein in InterPro
IPR020471 Aldo/keto_reductase
IPR023210 NADP_OxRdtase_dom
IPR036812 NADP_OxRdtase_dom_sf
PfamiView protein in Pfam
PF00248 Aldo_ket_red, 1 hit
PRINTSiPR00069 ALDKETRDTASE
SUPFAMiSSF51430 SSF51430, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiYAJO_ECOLI
AccessioniPrimary (citable) accession number: P77735
Secondary accession number(s): Q2MC07
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 15, 1998
Last modified: November 7, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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