UniProtKB - P77735 (YAJO_ECOLI)
Protein
1-deoxyxylulose-5-phosphate synthase YajO
Gene
yajO
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the conversion of ribulose 5-phosphate (Ru5P) to 1-deoxy-D-xylulose 5-phosphate (DXP), providing a direct route from pentoses to terpenes. May play a role in biosynthesis of DXP under conditions of thiamine starvation.1 Publication
Miscellaneous
Overexpression supports cell viability in the absence of the essential dxs-encoded 1-deoxy-D-xylulose-5-phosphate synthase (PubMed:25326299). Overexpression confers resistance to the 4-amino-2-methyl 5-hydroxymethylpyrimidine (HMP) analogs bacimethrin and 4-amino-2-trifluoromethyl 5-hydroxymethylpyrimidine (CF3-HMP) (PubMed:15292217).2 Publications
Catalytic activityi
Activity regulationi
NADH, NADPH or ATP do not increase activity.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 61 | Proton donorBy similarity | 1 |
GO - Molecular functioni
- D-threo-aldose 1-dehydrogenase activity Source: InterPro
GO - Biological processi
- thiamine metabolic process Source: EcoliWiki
Keywordsi
Molecular function | Oxidoreductase |
Enzyme and pathway databases
BioCyci | EcoCyc:G6236-MONOMER MetaCyc:G6236-MONOMER |
Protein family/group databases
TCDBi | 8.A.5.1.6, the voltage-gated k(+) channel Beta-subunit (kvBeta) family |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:yajO Ordered Locus Names:b0419, JW0409 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000070392 | 1 – 324 | 1-deoxyxylulose-5-phosphate synthase YajOAdd BLAST | 324 |
Proteomic databases
jPOSTi | P77735 |
PaxDbi | P77735 |
PRIDEi | P77735 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4259835, 111 interactors |
DIPi | DIP-11291N |
IntActi | P77735, 6 interactors |
STRINGi | 511145.b0419 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0667, Bacteria |
HOGENOMi | CLU_023205_2_0_6 |
InParanoidi | P77735 |
PhylomeDBi | P77735 |
Family and domain databases
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR020471, AKR IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
PRINTSi | PR00069, ALDKETRDTASE |
SUPFAMi | SSF51430, SSF51430, 1 hit |
i Sequence
Sequence statusi: Complete.
P77735-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQYNPLGKTD LRVSRLCLGC MTFGEPDRGN HAWTLPEESS RPIIKRALEG
60 70 80 90 100
GINFFDTANS YSDGSSEEIV GRALRDFARR EDVVVATKVF HRVGDLPEGL
110 120 130 140 150
SRAQILRSID DSLRRLGMDY VDILQIHRWD YNTPIEETLE ALNDVVKAGK
160 170 180 190 200
ARYIGASSMH ASQFAQALEL QKQHGWAQFV SMQDHYNLIY REEEREMLPL
210 220 230 240 250
CYQEGVAVIP WSPLARGRLT RPWGETTARL VSDEVGKNLY KESDENDAQI
260 270 280 290 300
AERLTGVSEE LGATRAQVAL AWLLSKPGIA APIIGTSREE QLDELLNAVD
310 320
ITLKPEQIAE LETPYKPHPV VGFK
Sequence cautioni
The sequence AAB40175 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U82664 Genomic DNA Translation: AAB40175.1 Different initiation. U00096 Genomic DNA Translation: AAC73522.2 AP009048 Genomic DNA Translation: BAE76199.1 |
PIRi | C64771 |
RefSeqi | NP_414953.2, NC_000913.3 WP_001199800.1, NZ_SSZK01000009.1 |
Genome annotation databases
EnsemblBacteriai | AAC73522; AAC73522; b0419 BAE76199; BAE76199; BAE76199 |
GeneIDi | 946903 |
KEGGi | ecj:JW0409 eco:b0419 |
PATRICi | fig|1411691.4.peg.1858 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U82664 Genomic DNA Translation: AAB40175.1 Different initiation. U00096 Genomic DNA Translation: AAC73522.2 AP009048 Genomic DNA Translation: BAE76199.1 |
PIRi | C64771 |
RefSeqi | NP_414953.2, NC_000913.3 WP_001199800.1, NZ_SSZK01000009.1 |
3D structure databases
SMRi | P77735 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4259835, 111 interactors |
DIPi | DIP-11291N |
IntActi | P77735, 6 interactors |
STRINGi | 511145.b0419 |
Protein family/group databases
TCDBi | 8.A.5.1.6, the voltage-gated k(+) channel Beta-subunit (kvBeta) family |
Proteomic databases
jPOSTi | P77735 |
PaxDbi | P77735 |
PRIDEi | P77735 |
Genome annotation databases
EnsemblBacteriai | AAC73522; AAC73522; b0419 BAE76199; BAE76199; BAE76199 |
GeneIDi | 946903 |
KEGGi | ecj:JW0409 eco:b0419 |
PATRICi | fig|1411691.4.peg.1858 |
Organism-specific databases
EchoBASEi | EB3377 |
Phylogenomic databases
eggNOGi | COG0667, Bacteria |
HOGENOMi | CLU_023205_2_0_6 |
InParanoidi | P77735 |
PhylomeDBi | P77735 |
Enzyme and pathway databases
BioCyci | EcoCyc:G6236-MONOMER MetaCyc:G6236-MONOMER |
Miscellaneous databases
PROi | PR:P77735 |
Family and domain databases
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR020471, AKR IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
PRINTSi | PR00069, ALDKETRDTASE |
SUPFAMi | SSF51430, SSF51430, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | YAJO_ECOLI | |
Accessioni | P77735Primary (citable) accession number: P77735 Secondary accession number(s): Q2MC07 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | December 15, 1998 | |
Last modified: | April 7, 2021 | |
This is version 142 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families