UniProtKB - P77718 (THII_ECOLI)
Protein
tRNA sulfurtransferase
Gene
thiI
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.3 Publications
Catalytic activityi
- [ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + a uridine in tRNA + ATP + H+ + 2 reduced [2Fe-2S]-[ferredoxin] = [ThiI sulfur-carrier protein]-L-cysteine + a 4-thiouridine in tRNA + AMP + diphosphate + 2 oxidized [2Fe-2S]-[ferredoxin]1 PublicationEC:2.8.1.41 Publication
Kineticsi
- KM=0.24 mM for ATP1 Publication
- KM=0.84 mM for GTP1 Publication
- Vmax=1.9 nmol/min/mg enzyme with ATP as cosubstrate1 Publication
- Vmax=1.29 nmol/min/mg enzyme with GTP as cosubstrate1 Publication
: thiamine diphosphate biosynthesis Pathwayi
This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 265 | ATPBy similarity | 1 | |
Binding sitei | 287 | ATP; via amide nitrogenBy similarity | 1 | |
Binding sitei | 296 | ATPBy similarity | 1 | |
Active sitei | 456 | Cysteine persulfide intermediate | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 183 – 184 | ATPBy similarity | 2 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- sulfurtransferase activity Source: UniProtKB-UniRule
- tRNA adenylyltransferase activity Source: InterPro
- tRNA binding Source: EcoCyc
GO - Biological processi
- thiamine biosynthetic process Source: EcoCyc
- thiamine diphosphate biosynthetic process Source: UniProtKB-UniPathway
- thiazole biosynthetic process Source: GO_Central
- tRNA 4-thiouridine biosynthesis Source: EcoCyc
Keywordsi
Molecular function | RNA-binding, Transferase, tRNA-binding |
Biological process | Thiamine biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:THII-MONOMER MetaCyc:THII-MONOMER |
SABIO-RKi | P77718 |
UniPathwayi | UPA00060 |
Names & Taxonomyi
Protein namesi | Recommended name: tRNA sulfurtransferase (EC:2.8.1.4)Alternative name(s): Sulfur carrier protein ThiS sulfurtransferase Thiamine biosynthesis protein ThiI tRNA 4-thiouridine synthase |
Gene namesi | Name:thiI Synonyms:yajK Ordered Locus Names:b0423, JW0413 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
Cytosol
- cytosol Source: EcoCyc
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 108 | C → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 189 | D → A: No activity. 1 Publication | 1 | |
Mutagenesisi | 202 | C → A: No effect. 1 Publication | 1 | |
Mutagenesisi | 207 | C → A: Enables partial functional complementation in vivo. Four-fold reduction in activity. 1 Publication | 1 | |
Mutagenesisi | 321 | K → M: No activity. 1 Publication | 1 | |
Mutagenesisi | 321 | K → R: Approximately half of wild-type activity. 1 Publication | 1 | |
Mutagenesisi | 344 | C → A: Cannot functionally complement for wild-type enzyme in vivo. 2700-fold reduction in activity. 1 Publication | 1 | |
Mutagenesisi | 456 | C → A: No activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000154837 | 1 – 482 | tRNA sulfurtransferaseAdd BLAST | 482 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 344 ↔ 456 | Redox-active1 Publication |
Keywords - PTMi
Disulfide bondProteomic databases
jPOSTi | P77718 |
PaxDbi | P77718 |
PRIDEi | P77718 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4263344, 4 interactors |
DIPi | DIP-10985N |
IntActi | P77718, 13 interactors |
STRINGi | 511145.b0423 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 61 – 165 | THUMPAdd BLAST | 105 | |
Domaini | 404 – 482 | RhodaneseAdd BLAST | 79 |
Sequence similaritiesi
Belongs to the ThiI family.Curated
Keywords - Domaini
Redox-active centerPhylogenomic databases
eggNOGi | COG0301, Bacteria COG0607, Bacteria |
HOGENOMi | CLU_037952_4_1_6 |
InParanoidi | P77718 |
PhylomeDBi | P77718 |
Family and domain databases
CDDi | cd01712, ThiI, 1 hit |
Gene3Di | 3.40.250.10, 1 hit 3.40.50.620, 1 hit |
HAMAPi | MF_00021, ThiI, 1 hit |
InterProi | View protein in InterPro IPR001763, Rhodanese-like_dom IPR036873, Rhodanese-like_dom_sf IPR014729, Rossmann-like_a/b/a_fold IPR026340, Thiazole_biosynth_dom IPR020536, ThiI_AANH IPR004114, THUMP_dom IPR003720, tRNA_STrfase |
Pfami | View protein in Pfam PF02568, ThiI, 1 hit PF02926, THUMP, 1 hit |
SMARTi | View protein in SMART SM00981, THUMP, 1 hit |
SUPFAMi | SSF52821, SSF52821, 1 hit |
TIGRFAMsi | TIGR04271, ThiI_C_thiazole, 1 hit TIGR00342, TIGR00342, 1 hit |
PROSITEi | View protein in PROSITE PS50206, RHODANESE_3, 1 hit PS51165, THUMP, 1 hit |
i Sequence
Sequence statusi: Complete.
P77718-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKFIIKLFPE ITIKSQSVRL RFIKILTGNI RNVLKHYDET LAVVRHWDNI
60 70 80 90 100
EVRAKDENQR LAIRDALTRI PGIHHILEVE DVPFTDMHDI FEKALVQYRD
110 120 130 140 150
QLEGKTFCVR VKRRGKHDFS SIDVERYVGG GLNQHIESAR VKLTNPDVTV
160 170 180 190 200
HLEVEDDRLL LIKGRYEGIG GFPIGTQEDV LSLISGGFDS GVSSYMLMRR
210 220 230 240 250
GCRVHYCFFN LGGAAHEIGV RQVAHYLWNR FGSSHRVRFV AINFEPVVGE
260 270 280 290 300
ILEKIDDGQM GVILKRMMVR AASKVAERYG VQALVTGEAL GQVSSQTLTN
310 320 330 340 350
LRLIDNVSDT LILRPLISYD KEHIINLARQ IGTEDFARTM PEYCGVISKS
360 370 380 390 400
PTVKAVKSKI EAEEEKFDFS ILDKVVEEAN NVDIREIAQQ TEQEVVEVET
410 420 430 440 450
VNGFGPNDVI LDIRSIDEQE DKPLKVEGID VVSLPFYKLS TKFGDLDQNK
460 470 480
TWLLWCERGV MSRLQALYLR EQGFNNVKVY RP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U82664 Genomic DNA Translation: AAB40179.1 U00096 Genomic DNA Translation: AAC73526.1 AP009048 Genomic DNA Translation: BAE76203.1 U34923 Genomic DNA No translation available. |
PIRi | G64771 |
RefSeqi | NP_414957.1, NC_000913.3 WP_000668662.1, NZ_SSUW01000008.1 |
Genome annotation databases
EnsemblBacteriai | AAC73526; AAC73526; b0423 BAE76203; BAE76203; BAE76203 |
GeneIDi | 945067 |
KEGGi | ecj:JW0413 eco:b0423 |
PATRICi | fig|1411691.4.peg.1854 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U82664 Genomic DNA Translation: AAB40179.1 U00096 Genomic DNA Translation: AAC73526.1 AP009048 Genomic DNA Translation: BAE76203.1 U34923 Genomic DNA No translation available. |
PIRi | G64771 |
RefSeqi | NP_414957.1, NC_000913.3 WP_000668662.1, NZ_SSUW01000008.1 |
3D structure databases
SMRi | P77718 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263344, 4 interactors |
DIPi | DIP-10985N |
IntActi | P77718, 13 interactors |
STRINGi | 511145.b0423 |
Proteomic databases
jPOSTi | P77718 |
PaxDbi | P77718 |
PRIDEi | P77718 |
Genome annotation databases
EnsemblBacteriai | AAC73526; AAC73526; b0423 BAE76203; BAE76203; BAE76203 |
GeneIDi | 945067 |
KEGGi | ecj:JW0413 eco:b0423 |
PATRICi | fig|1411691.4.peg.1854 |
Organism-specific databases
EchoBASEi | EB3058 |
Phylogenomic databases
eggNOGi | COG0301, Bacteria COG0607, Bacteria |
HOGENOMi | CLU_037952_4_1_6 |
InParanoidi | P77718 |
PhylomeDBi | P77718 |
Enzyme and pathway databases
UniPathwayi | UPA00060 |
BioCyci | EcoCyc:THII-MONOMER MetaCyc:THII-MONOMER |
SABIO-RKi | P77718 |
Miscellaneous databases
PROi | PR:P77718 |
Family and domain databases
CDDi | cd01712, ThiI, 1 hit |
Gene3Di | 3.40.250.10, 1 hit 3.40.50.620, 1 hit |
HAMAPi | MF_00021, ThiI, 1 hit |
InterProi | View protein in InterPro IPR001763, Rhodanese-like_dom IPR036873, Rhodanese-like_dom_sf IPR014729, Rossmann-like_a/b/a_fold IPR026340, Thiazole_biosynth_dom IPR020536, ThiI_AANH IPR004114, THUMP_dom IPR003720, tRNA_STrfase |
Pfami | View protein in Pfam PF02568, ThiI, 1 hit PF02926, THUMP, 1 hit |
SMARTi | View protein in SMART SM00981, THUMP, 1 hit |
SUPFAMi | SSF52821, SSF52821, 1 hit |
TIGRFAMsi | TIGR04271, ThiI_C_thiazole, 1 hit TIGR00342, TIGR00342, 1 hit |
PROSITEi | View protein in PROSITE PS50206, RHODANESE_3, 1 hit PS51165, THUMP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | THII_ECOLI | |
Accessioni | P77718Primary (citable) accession number: P77718 Secondary accession number(s): Q2MC03 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | April 7, 2021 | |
This is version 159 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families