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Protein

Long-chain acyl-CoA thioesterase FadM

Gene

fadM

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Long-chain acyl-CoA thioesterase with a preference for 3,5-tetradecadienoyl-CoA. Could be involved in beta-oxidation of fatty acids.1 Publication

Kineticsi

  1. KM=3.0 µM for 3,5-cis-tetradecadienoyl-CoA1 Publication
  2. KM=10.8 µM for 3,5-cis-dodecadienoyl-CoA1 Publication
  3. KM=5.8 µM for 3-hydroxytetradecanoyl-CoA1 Publication
  4. KM=6.0 µM for palmitoyl-CoA1 Publication
  5. KM=6.3 µM for myristoyl-CoA1 Publication
  6. KM=13.0 µM for lauroyl-CoA1 Publication
  1. Vmax=101.3 µmol/min/mg enzyme with 3,5-cis-tetradecadienoyl-CoA as substrate1 Publication
  2. Vmax=92.3 µmol/min/mg enzyme with 3,5-cis-dodecadienoyl-CoA as substrate1 Publication
  3. Vmax=45.5 µmol/min/mg enzyme with 3-hydroxytetradecanoyl-CoA as substrate1 Publication
  4. Vmax=43.5 µmol/min/mg enzyme with palmitoyl-CoA as substrate1 Publication
  5. Vmax=26.4 µmol/min/mg enzyme with myristoyl-CoA as substrate1 Publication
  6. Vmax=6.9 µmol/min/mg enzyme with lauroyl-CoA as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei13Sequence analysis1

GO - Molecular functioni

  • acyl-CoA hydrolase activity Source: EcoCyc

GO - Biological processi

  • fatty acid beta-oxidation Source: EcoCyc

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyciEcoCyc:G6244-MONOMER
MetaCyc:G6244-MONOMER
BRENDAi3.1.2.20 2026

Chemistry databases

SwissLipidsiSLP:000001801

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain acyl-CoA thioesterase FadM (EC:3.1.2.-)
Alternative name(s):
Acyl-CoA thioester hydrolase
Thioesterase 3
Thioesterase III1 Publication
Gene namesi
Name:fadM
Synonyms:tesC, ybaW
Ordered Locus Names:b0443, JW0433
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13251 fadM

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001686301 – 132Long-chain acyl-CoA thioesterase FadMAdd BLAST132

Proteomic databases

PaxDbiP77712
PRIDEiP77712

Expressioni

Inductioni

Up-regulated by growth on oleic acid. Repressed by FadR and by the cyclic AMP receptor protein-cAMP (CRP-cAMP) complex.2 Publications

Interactioni

Subunit structurei

Homotetramer.Curated

Protein-protein interaction databases

BioGridi4259836, 162 interactors
IntActiP77712, 4 interactors
STRINGi316385.ECDH10B_0399

Structurei

Secondary structure

1132
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi1 – 6Combined sources6
Helixi9 – 11Combined sources3
Beta strandi16 – 18Combined sources3
Helixi20 – 36Combined sources17
Helixi39 – 46Combined sources8
Beta strandi49 – 60Combined sources12
Beta strandi69 – 80Combined sources12
Beta strandi83 – 92Combined sources10
Turni93 – 96Combined sources4
Beta strandi97 – 110Combined sources14
Turni111 – 113Combined sources3
Beta strandi115 – 117Combined sources3
Turni121 – 124Combined sources4
Helixi125 – 131Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NJKX-ray1.90A/B/C/D1-132[»]
ProteinModelPortaliP77712
SMRiP77712
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP77712

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108TGP Bacteria
COG0824 LUCA
HOGENOMiHOG000004132
InParanoidiP77712
KOiK12500
OMAiLFQHVNN

Family and domain databases

InterProiView protein in InterPro
IPR029069 HotDog_dom_sf
IPR006684 YbgC/YbaW
PIRSFiPIRSF003230 YbgC, 1 hit
SUPFAMiSSF54637 SSF54637, 1 hit
TIGRFAMsiTIGR00051 TIGR00051, 1 hit

Sequencei

Sequence statusi: Complete.

P77712-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQTQIKVRGY HLDVYQHVNN ARYLEFLEEA RWDGLENSDS FQWMTAHNIA
60 70 80 90 100
FVVVNININY RRPAVLSDLL TITSQLQQLN GKSGILSQVI TLEPEGQVVA
110 120 130
DALITFVCID LKTQKALALE GELREKLEQM VK
Length:132
Mass (Da):15,088
Last modified:February 1, 1997 - v1
Checksum:iEBB3539149A37383
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D82943 Genomic DNA Translation: BAA11647.1
U82664 Genomic DNA Translation: AAB40199.1
U00096 Genomic DNA Translation: AAC73546.1
AP009048 Genomic DNA Translation: BAE76223.1
PIRiC64774
RefSeqiNP_414977.1, NC_000913.3
WP_001194534.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73546; AAC73546; b0443
BAE76223; BAE76223; BAE76223
GeneIDi945812
KEGGiecj:JW0433
eco:b0443
PATRICifig|1411691.4.peg.1833

Similar proteinsi

Entry informationi

Entry nameiFADM_ECOLI
AccessioniPrimary (citable) accession number: P77712
Secondary accession number(s): Q2MBY3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: February 1, 1997
Last modified: March 28, 2018
This is version 127 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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