UniProtKB - P77619 (YFEW_ECOLI)
Protein
Putative D-alanyl-D-alanine carboxypeptidase
Gene
yfeW
Organism
Escherichia coli (strain K12)
Status
Functioni
Penicillin-binding protein. Has low DD-carboxypeptidase activity.1 Publication
Catalytic activityi
- Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.UniRule annotation1 Publication EC:3.4.16.4
GO - Molecular functioni
- penicillin binding Source: EcoCyc
- serine-type D-Ala-D-Ala carboxypeptidase activity Source: EcoCyc
Keywordsi
Molecular function | Carboxypeptidase, Hydrolase, Protease |
Enzyme and pathway databases
BioCyci | EcoCyc:G7265-MONOMER MetaCyc:G7265-MONOMER |
Protein family/group databases
MEROPSi | S12.A03 |
Names & Taxonomyi
Protein namesi | Recommended name: Putative D-alanyl-D-alanine carboxypeptidaseUniRule annotationCurated (EC:3.4.16.4UniRule annotation1 Publication)Alternative name(s): DD-carboxypeptidase1 PublicationUniRule annotation Short name: DD-CPase1 PublicationUniRule annotation Penicillin binding protein 4BCurated |
Gene namesi | Name:yfeWUniRule annotation Synonyms:pbp4B1 Publication Ordered Locus Names:b2430, JW5395 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane UniRule annotation1 Publication; Single-pass membrane protein UniRule annotation1 Publication
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 7 – 25 | Helical; Signal-anchorUniRule annotation1 PublicationAdd BLAST | 19 |
GO - Cellular componenti
- integral component of membrane Source: UniProtKB-KW
- intrinsic component of plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Not essential for cell growth.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000036256 | 1 – 434 | Putative D-alanyl-D-alanine carboxypeptidaseAdd BLAST | 434 |
Proteomic databases
jPOSTi | P77619 |
PaxDbi | P77619 |
PRIDEi | P77619 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260747, 340 interactors |
DIPi | DIP-12021N |
STRINGi | 511145.b2430 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG1680, Bacteria |
HOGENOMi | CLU_020027_1_2_6 |
InParanoidi | P77619 |
PhylomeDBi | P77619 |
Family and domain databases
Gene3Di | 3.40.710.10, 1 hit |
HAMAPi | MF_01034, S12_YfeW, 1 hit |
InterProi | View protein in InterPro IPR001466, Beta-lactam-related IPR012338, Beta-lactam/transpept-like IPR022849, Pept_S12_YfeW/YbbE-like |
Pfami | View protein in Pfam PF00144, Beta-lactamase, 1 hit |
SUPFAMi | SSF56601, SSF56601, 1 hit |
PROSITEi | View protein in PROSITE PS51257, PROKAR_LIPOPROTEIN, 1 hit |
i Sequence
Sequence statusi: Complete.
P77619-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKRTMLYLSL LAVSCSVSAA KYPVLTESSP EKAGFNVERL NQMDRWISQQ
60 70 80 90 100
VDVGYPSVNL LIIKDNQIVY RKAWGAAKKY DGSVLMEQPV KATTGTLYDL
110 120 130 140 150
ASNTKMYATN FALQKLMSEG KLHPDDRIAK YIPGFADSPN DTIKGKNTLR
160 170 180 190 200
ISDLLHHSGG FPADPQYPNK AVAGALYSQD KGQTLEMIKR TPLEYQPGSK
210 220 230 240 250
HIYSDVDYML LGFIVESVTG QPLDRYVEES IYRPLGLTHT VFNPLLKGFK
260 270 280 290 300
PQQIAATELN GNTRDGVIHF PNIRTSTLWG QVHDEKAFYS MGGVSGHAGL
310 320 330 340 350
FSNTGDIAVL MQTMLNGGGY GDVQLFNAET VKMFTTSSKE DATFGLGWRV
360 370 380 390 400
NGNATMTPTF GTLASPQTYG HTGWTGTVTV IDPVNHMTIV MLSNKPHSPV
410 420 430
ADPQKNPNMF ESGQLPIATY GWVVDQVYAA LKQK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75483.2 AP009048 Genomic DNA Translation: BAA16314.2 |
PIRi | E65017 |
RefSeqi | NP_416925.2, NC_000913.3 WP_001327042.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75483; AAC75483; b2430 BAA16314; BAA16314; BAA16314 |
GeneIDi | 946907 |
KEGGi | ecj:JW5395 eco:b2430 |
PATRICi | fig|1411691.4.peg.4301 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75483.2 AP009048 Genomic DNA Translation: BAA16314.2 |
PIRi | E65017 |
RefSeqi | NP_416925.2, NC_000913.3 WP_001327042.1, NZ_LN832404.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4260747, 340 interactors |
DIPi | DIP-12021N |
STRINGi | 511145.b2430 |
Protein family/group databases
MEROPSi | S12.A03 |
Proteomic databases
jPOSTi | P77619 |
PaxDbi | P77619 |
PRIDEi | P77619 |
Genome annotation databases
EnsemblBacteriai | AAC75483; AAC75483; b2430 BAA16314; BAA16314; BAA16314 |
GeneIDi | 946907 |
KEGGi | ecj:JW5395 eco:b2430 |
PATRICi | fig|1411691.4.peg.4301 |
Organism-specific databases
EchoBASEi | EB3916 |
Phylogenomic databases
eggNOGi | COG1680, Bacteria |
HOGENOMi | CLU_020027_1_2_6 |
InParanoidi | P77619 |
PhylomeDBi | P77619 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7265-MONOMER MetaCyc:G7265-MONOMER |
Miscellaneous databases
PROi | PR:P77619 |
Family and domain databases
Gene3Di | 3.40.710.10, 1 hit |
HAMAPi | MF_01034, S12_YfeW, 1 hit |
InterProi | View protein in InterPro IPR001466, Beta-lactam-related IPR012338, Beta-lactam/transpept-like IPR022849, Pept_S12_YfeW/YbbE-like |
Pfami | View protein in Pfam PF00144, Beta-lactamase, 1 hit |
SUPFAMi | SSF56601, SSF56601, 1 hit |
PROSITEi | View protein in PROSITE PS51257, PROKAR_LIPOPROTEIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | YFEW_ECOLI | |
Accessioni | P77619Primary (citable) accession number: P77619 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | December 15, 1998 | |
Last modified: | December 2, 2020 | |
This is version 136 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries