UniProtKB - P77589 (MHPT_ECOLI)
Protein
3-(3-hydroxy-phenyl)propionate transporter
Gene
mhpT
Organism
Escherichia coli (strain K12)
Status
Functioni
Uptake of 3-(3-hydroxyphenyl)propionate (3HPP) across the cytoplasmic membrane. Transport is driven by the proton motive force. Does not transport benzoate, 3-hydroxybenzoate or gentisate.1 Publication
GO - Molecular functioni
- symporter activity Source: UniProtKB-KW
Keywordsi
Biological process | Symport, Transport |
Enzyme and pathway databases
BioCyci | EcoCyc:MHPT-MONOMER MetaCyc:MHPT-MONOMER |
Protein family/group databases
TCDBi | 2.A.1.15.2, the major facilitator superfamily (mfs) |
Names & Taxonomyi
Protein namesi | Recommended name: 3-(3-hydroxy-phenyl)propionate transporterShort name: 3HPP transporter Alternative name(s): 3-(3-hydroxy-phenyl)propionate:H(+) symporter Short name: 3HPP:H(+) symporter |
Gene namesi | Name:mhpT Synonyms:yaiK Ordered Locus Names:b0353, JW5046 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 2 Publications; Multi-pass membrane protein 2 Publications
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 16 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 17 – 37 | Helical; Name=1Sequence analysisAdd BLAST | 21 | |
Topological domaini | 38 – 53 | PeriplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 54 – 74 | Helical; Name=2Sequence analysisAdd BLAST | 21 | |
Topological domaini | 75 – 81 | CytoplasmicSequence analysis | 7 | |
Transmembranei | 82 – 102 | Helical; Name=3Sequence analysisAdd BLAST | 21 | |
Topological domaini | 103 – 105 | PeriplasmicSequence analysis | 3 | |
Transmembranei | 106 – 126 | Helical; Name=4Sequence analysisAdd BLAST | 21 | |
Topological domaini | 127 – 142 | CytoplasmicSequence analysisAdd BLAST | 16 | |
Transmembranei | 143 – 163 | Helical; Name=5Sequence analysisAdd BLAST | 21 | |
Topological domaini | 164 | PeriplasmicSequence analysis | 1 | |
Transmembranei | 165 – 185 | Helical; Name=6Sequence analysisAdd BLAST | 21 | |
Topological domaini | 186 – 217 | CytoplasmicSequence analysisAdd BLAST | 32 | |
Transmembranei | 218 – 238 | Helical; Name=7Sequence analysisAdd BLAST | 21 | |
Topological domaini | 239 – 253 | PeriplasmicSequence analysisAdd BLAST | 15 | |
Transmembranei | 254 – 274 | Helical; Name=8Sequence analysisAdd BLAST | 21 | |
Topological domaini | 275 – 279 | CytoplasmicSequence analysis | 5 | |
Transmembranei | 280 – 300 | Helical; Name=9Sequence analysisAdd BLAST | 21 | |
Topological domaini | 301 – 306 | PeriplasmicSequence analysis | 6 | |
Transmembranei | 307 – 327 | Helical; Name=10Sequence analysisAdd BLAST | 21 | |
Topological domaini | 328 – 339 | CytoplasmicSequence analysisAdd BLAST | 12 | |
Transmembranei | 340 – 360 | Helical; Name=11Sequence analysisAdd BLAST | 21 | |
Topological domaini | 361 – 369 | PeriplasmicSequence analysis | 9 | |
Transmembranei | 370 – 390 | Helical; Name=12Sequence analysisAdd BLAST | 21 | |
Topological domaini | 391 – 403 | CytoplasmicSequence analysisAdd BLAST | 13 |
GO - Cellular componenti
- integral component of membrane Source: GO_Central
- plasma membrane Source: EcoCyc
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Deletion mutant loses the ability to utilize 3HPP at pH 8.2.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 27 | E → A: Lack of 3HPP transport activity. 1 Publication | 1 | |
Mutagenesisi | 27 | E → D: Slight decrease in 3HPP transport activity. 1 Publication | 1 | |
Mutagenesisi | 75 | D → A or E: Lack of 3HPP transport activity. 1 Publication | 1 | |
Mutagenesisi | 272 | A → H: 30% increase in 3HPP transport activity. 1 Publication | 1 | |
Mutagenesisi | 276 | K → D: Lack of 3HPP transport activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000050308 | 1 – 403 | 3-(3-hydroxy-phenyl)propionate transporterAdd BLAST | 403 |
Proteomic databases
PaxDbi | P77589 |
PRIDEi | P77589 |
Expressioni
Inductioni
Induced by 3HPP.1 Publication
Interactioni
Protein-protein interaction databases
BioGRIDi | 4261625, 23 interactors |
STRINGi | 511145.b0353 |
Family & Domainsi
Sequence similaritiesi
Belongs to the major facilitator superfamily. Aromatic acid:H(+) symporter (AAHS) (TC 2.A.1.15) family. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | COG2814, Bacteria |
HOGENOMi | CLU_001265_46_4_6 |
InParanoidi | P77589 |
PhylomeDBi | P77589 |
Family and domain databases
InterProi | View protein in InterPro IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf IPR005829, Sugar_transporter_CS |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
i Sequence
Sequence statusi: Complete.
P77589-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSTRTPSSSS SRLMLTIGLC FLVALMEGLD LQAAGIAAGG IAQAFALDKM
60 70 80 90 100
QMGWIFSAGI LGLLPGALVG GMLADRYGRK RILIGSVALF GLFSLATAIA
110 120 130 140 150
WDFPSLVFAR LMTGVGLGAA LPNLIALTSE AAGPRFRGTA VSLMYCGVPI
160 170 180 190 200
GAALAATLGF AGANLAWQTV FWVGGVVPLI LVPLLMRWLP ESAVFAGEKQ
210 220 230 240 250
SAPPLRALFA PETATATLLL WLCYFFTLLV VYMLINWLPL LLVEQGFQPS
260 270 280 290 300
QAAGVMFALQ MGAASGTLML GALMDKLRPV TMSLLIYSGM LASLLALGTV
310 320 330 340 350
SSFNGMLLAG FVAGLFATGG QSVLYALAPL FYSSQIRATG VGTAVAVGRL
360 370 380 390 400
GAMSGPLLAG KMLALGTGTV GVMAASAPGI LVAGLAVFIL MSRRSRIQPC
ADA
Sequence cautioni
The sequence AAB18077 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated
The sequence D85613 differs from that shown. Reason: Frameshift.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 295 | L → V in D85613 (PubMed:9098055).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D85613 Genomic DNA No translation available. U73857 Genomic DNA Translation: AAB18077.1 Different initiation. U00096 Genomic DNA Translation: AAC73456.2 AP009048 Genomic DNA Translation: BAE76135.1 X97543 Genomic DNA Translation: CAA66145.1 |
PIRi | A64763 |
RefSeqi | NP_414887.2, NC_000913.3 WP_000107627.1, NZ_SSZK01000009.1 |
Genome annotation databases
EnsemblBacteriai | AAC73456; AAC73456; b0353 BAE76135; BAE76135; BAE76135 |
GeneIDi | 944997 |
KEGGi | ecj:JW5046 eco:b0353 |
PATRICi | fig|1411691.4.peg.1925 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D85613 Genomic DNA No translation available. U73857 Genomic DNA Translation: AAB18077.1 Different initiation. U00096 Genomic DNA Translation: AAC73456.2 AP009048 Genomic DNA Translation: BAE76135.1 X97543 Genomic DNA Translation: CAA66145.1 |
PIRi | A64763 |
RefSeqi | NP_414887.2, NC_000913.3 WP_000107627.1, NZ_SSZK01000009.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 4261625, 23 interactors |
STRINGi | 511145.b0353 |
Protein family/group databases
TCDBi | 2.A.1.15.2, the major facilitator superfamily (mfs) |
Proteomic databases
PaxDbi | P77589 |
PRIDEi | P77589 |
Genome annotation databases
EnsemblBacteriai | AAC73456; AAC73456; b0353 BAE76135; BAE76135; BAE76135 |
GeneIDi | 944997 |
KEGGi | ecj:JW5046 eco:b0353 |
PATRICi | fig|1411691.4.peg.1925 |
Organism-specific databases
EchoBASEi | EB3078 |
Phylogenomic databases
eggNOGi | COG2814, Bacteria |
HOGENOMi | CLU_001265_46_4_6 |
InParanoidi | P77589 |
PhylomeDBi | P77589 |
Enzyme and pathway databases
BioCyci | EcoCyc:MHPT-MONOMER MetaCyc:MHPT-MONOMER |
Miscellaneous databases
PROi | PR:P77589 |
Family and domain databases
InterProi | View protein in InterPro IPR011701, MFS IPR020846, MFS_dom IPR036259, MFS_trans_sf IPR005829, Sugar_transporter_CS |
Pfami | View protein in Pfam PF07690, MFS_1, 1 hit |
SUPFAMi | SSF103473, SSF103473, 1 hit |
PROSITEi | View protein in PROSITE PS50850, MFS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MHPT_ECOLI | |
Accessioni | P77589Primary (citable) accession number: P77589 Secondary accession number(s): P77037, Q2MC71 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | May 30, 2000 | |
Last modified: | December 2, 2020 | |
This is version 151 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families