UniProtKB - P77570 (ANMK_ECOLI)
Protein
Anhydro-N-acetylmuramic acid kinase
Gene
anmK
Organism
Escherichia coli (strain K12)
Status
Functioni
Catalyzes the specific phosphorylation of 1,6-anhydro-N-acetylmuramic acid (anhMurNAc) with the simultaneous cleavage of the 1,6-anhydro ring, generating MurNAc-6-P. Is required for the utilization of anhMurNAc either imported from the medium or derived from its own cell wall murein, and thus plays a role in cell wall recycling.2 Publications
Catalytic activityi
- 1,6-anhydro-N-acetyl-β-muramate + ATP + H2O = ADP + H+ + N-acetyl-D-muramate 6-phosphate1 PublicationEC:2.7.1.1701 Publication
Activity regulationi
Strongly inhibited by ADP.1 Publication
Kineticsi
- KM=1 mM for anhMurNAc1 Publication
- KM=1 mM for ATP1 Publication
- Vmax=180 µmol/min/mg enzyme1 Publication
pH dependencei
Optimum pH is 10-11. Inactive at pH 4.5.1 Publication
: 1,6-anhydro-N-acetylmuramate degradation Pathwayi
This protein is involved in the pathway 1,6-anhydro-N-acetylmuramate degradation, which is part of Amino-sugar metabolism.View all proteins of this organism that are known to be involved in the pathway 1,6-anhydro-N-acetylmuramate degradation and in Amino-sugar metabolism.
Pathwayi: peptidoglycan recycling
This protein is involved in the pathway peptidoglycan recycling, which is part of Cell wall biogenesis.View all proteins of this organism that are known to be involved in the pathway peptidoglycan recycling and in Cell wall biogenesis.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 12 – 19 | ATPSequence analysis | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- kinase activity Source: EcoCyc
- phosphotransferase activity, alcohol group as acceptor Source: UniProtKB-UniRule
GO - Biological processi
- 1,6-anhydro-N-acetyl-beta-muramic acid catabolic process Source: UniProtKB-UniPathway
- amino sugar metabolic process Source: UniProtKB-UniRule
- carbohydrate metabolic process Source: UniProtKB-KW
- peptidoglycan turnover Source: UniProtKB-UniRule
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Carbohydrate metabolism |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:G6880-MONOMER MetaCyc:G6880-MONOMER |
SABIO-RKi | P77570 |
UniPathwayi | UPA00343 UPA00544 |
Names & Taxonomyi
Protein namesi | Recommended name: Anhydro-N-acetylmuramic acid kinase (EC:2.7.1.170)Alternative name(s): AnhMurNAc kinase |
Gene namesi | Name:anmK Synonyms:ydhH Ordered Locus Names:b1640, JW1632 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000214821 | 1 – 369 | Anhydro-N-acetylmuramic acid kinaseAdd BLAST | 369 |
Proteomic databases
jPOSTi | P77570 |
PaxDbi | P77570 |
PRIDEi | P77570 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4263491, 14 interactors |
IntActi | P77570, 7 interactors |
STRINGi | 511145.b1640 |
Family & Domainsi
Sequence similaritiesi
Belongs to the anhydro-N-acetylmuramic acid kinase family.Curated
Phylogenomic databases
eggNOGi | COG2377, Bacteria |
HOGENOMi | CLU_038782_0_0_6 |
InParanoidi | P77570 |
PhylomeDBi | P77570 |
Family and domain databases
HAMAPi | MF_01270, AnhMurNAc_kinase, 1 hit |
InterProi | View protein in InterPro IPR005338, Anhydro_N_Ac-Mur_kinase IPR043129, ATPase_NBD |
PANTHERi | PTHR30605, PTHR30605, 1 hit |
Pfami | View protein in Pfam PF03702, AnmK, 1 hit |
SUPFAMi | SSF53067, SSF53067, 1 hit |
i Sequence
Sequence statusi: Complete.
P77570-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKSGRFIGVM SGTSLDGVDV VLATIDEHRV AQLASLSWPI PVSLKQAVLD
60 70 80 90 100
ICQGQQLTLS QFGQLDTQLG QLFADAVNAL LKEQNLQARD IVAIGCHGQT
110 120 130 140 150
VWHEPTGVAP HTLQIGDNNQ IVARTGITVV GDFRRRDIAL GGQGAPLVPA
160 170 180 190 200
FHHALLAHPT ERRMVLNIGG IANLSLLIPG QPVGGYDTGP GNMLMDAWIW
210 220 230 240 250
RQAGKPYDKD AEWARAGKVI LPLLQNMLSD PYFSQPAPKS TGREYFNYGW
260 270 280 290 300
LERHLRHFPG VDPRDVQATL AELTAVTISE QVLLSGGCER LMVCGGGSRN
310 320 330 340 350
PLLMARLAAL LPGTEVTTTD AVGISGDDME ALAFAWLAWR TLAGLPGNLP
360
SVTGASQETV LGAIFPANP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74712.1 AP009048 Genomic DNA Translation: BAA15401.1 |
PIRi | B64921 |
RefSeqi | NP_416157.1, NC_000913.3 WP_000835039.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC74712; AAC74712; b1640 BAA15401; BAA15401; BAA15401 |
GeneIDi | 946810 |
KEGGi | ecj:JW1632 eco:b1640 |
PATRICi | fig|1411691.4.peg.620 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC74712.1 AP009048 Genomic DNA Translation: BAA15401.1 |
PIRi | B64921 |
RefSeqi | NP_416157.1, NC_000913.3 WP_000835039.1, NZ_LN832404.1 |
3D structure databases
SMRi | P77570 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 4263491, 14 interactors |
IntActi | P77570, 7 interactors |
STRINGi | 511145.b1640 |
Proteomic databases
jPOSTi | P77570 |
PaxDbi | P77570 |
PRIDEi | P77570 |
Genome annotation databases
EnsemblBacteriai | AAC74712; AAC74712; b1640 BAA15401; BAA15401; BAA15401 |
GeneIDi | 946810 |
KEGGi | ecj:JW1632 eco:b1640 |
PATRICi | fig|1411691.4.peg.620 |
Organism-specific databases
EchoBASEi | EB3700 |
Phylogenomic databases
eggNOGi | COG2377, Bacteria |
HOGENOMi | CLU_038782_0_0_6 |
InParanoidi | P77570 |
PhylomeDBi | P77570 |
Enzyme and pathway databases
UniPathwayi | UPA00343 UPA00544 |
BioCyci | EcoCyc:G6880-MONOMER MetaCyc:G6880-MONOMER |
SABIO-RKi | P77570 |
Miscellaneous databases
PROi | PR:P77570 |
Family and domain databases
HAMAPi | MF_01270, AnhMurNAc_kinase, 1 hit |
InterProi | View protein in InterPro IPR005338, Anhydro_N_Ac-Mur_kinase IPR043129, ATPase_NBD |
PANTHERi | PTHR30605, PTHR30605, 1 hit |
Pfami | View protein in Pfam PF03702, AnmK, 1 hit |
SUPFAMi | SSF53067, SSF53067, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ANMK_ECOLI | |
Accessioni | P77570Primary (citable) accession number: P77570 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | February 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 130 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families