anmK

Functionⁱ

Catalyzes the specific phosphorylation of 1,6-anhydro-N-acetylmuramic acid (anhMurNAc) with the simultaneous cleavage of the 1,6-anhydro ring, generating MurNAc-6-P. Is required for the utilization of anhMurNAc either imported from the medium or derived from its own cell wall murein, and thus plays a role in cell wall recycling.2 Publications

Catalytic activityⁱ

ATP + 1,6-anhydro-N-acetyl-beta-muramate + H₂O = ADP + N-acetylmuramate 6-phosphate.1 Publication

Enzyme regulationⁱ

Strongly inhibited by ADP.1 Publication

Kineticsⁱ

V_max=
180 µmol/min/mg enzyme
1 Publication
Manual assertion based on experiment inⁱ
- Ref.4
  "Recycling of the anhydro-N-acetylmuramic acid derived from cell wall murein involves a two-step conversion to N-acetylglucosamine-phosphate."
  Uehara T., Suefuji K., Valbuena N., Meehan B., Donegan M., Park J.T.
  J. Bacteriol. 187:3643-3649(2005) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, CATALYTIC ACTIVITY, IDENTIFICATION BY MASS SPECTROMETRY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.

pH dependenceⁱ

Optimum pH is 10-11. Inactive at pH 4.5.1 Publication

Pathwayⁱ: 1,6-anhydro-N-acetylmuramate degradation

This protein is involved in the pathway 1,6-anhydro-N-acetylmuramate degradation, which is part of Amino-sugar metabolism.
View all proteins of this organism that are known to be involved in the pathway 1,6-anhydro-N-acetylmuramate degradation and in Amino-sugar metabolism.

Pathwayⁱ: peptidoglycan recycling

This protein is involved in the pathway peptidoglycan recycling, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan recycling and in Cell wall biogenesis.

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	12 – 19	ATPSequence analysis		8

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
kinase activity
Source: EcoCyc
Inferred from direct assayⁱ
- 15901686
phosphotransferase activity, alcohol group as acceptor Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

1,6-anhydro-N-acetyl-beta-muramic acid catabolic process Source: UniProtKB-UniPathway
amino sugar metabolic process Source: InterPro
carbohydrate metabolic process Source: UniProtKB-KW
peptidoglycan turnover Source: UniProtKB-UniPathway

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Kinase, Transferase
Biological process	Carbohydrate metabolism
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	EcoCyc:G6880-MONOMER MetaCyc:G6880-MONOMER
SABIO-RKⁱ	P77570
UniPathwayⁱ	UPA00343 UPA00544

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Anhydro-N-acetylmuramic acid kinase (EC:2.7.1.170) Alternative name(s): AnhMurNAc kinase
Gene namesⁱ	Name:anmK Synonyms:ydhH Ordered Locus Names:b1640, JW1632
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG13942 anmK

EcoGeneⁱ

EG13942 anmK

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000214821	1 – 369	Anhydro-N-acetylmuramic acid kinaseAdd BLAST		369

Proteomic databases

PaxDbⁱ	P77570
PRIDEⁱ	P77570

Interactionⁱ

Protein-protein interaction databases

BioGridⁱ	4263491, 14 interactors
IntActⁱ	P77570, 7 interactors
STRINGⁱ	316385.ECDH10B_1774

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P77570
SMRⁱ	P77570
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the anhydro-N-acetylmuramic acid kinase family.Curated

Phylogenomic databases

eggNOGⁱ	ENOG4105ED7 Bacteria COG2377 LUCA
HOGENOMⁱ	HOG000256309
InParanoidⁱ	P77570
KEGG Orthology (KO) More...KOⁱ	K09001
OMAⁱ	HGQTVYH
PhylomeDBⁱ	P77570

Family and domain databases

HAMAPⁱ	MF_01270 AnhMurNAc_kinase, 1 hit
InterProⁱ	View protein in InterPro IPR005338 Anhydro_N_Ac-Mur_kinase
PANTHERⁱ	PTHR30605 PTHR30605, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF03702 AnmK, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P77570-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKSGRFIGVM SGTSLDGVDV VLATIDEHRV AQLASLSWPI PVSLKQAVLD 
        60         70         80         90        100
ICQGQQLTLS QFGQLDTQLG QLFADAVNAL LKEQNLQARD IVAIGCHGQT 
       110        120        130        140        150
VWHEPTGVAP HTLQIGDNNQ IVARTGITVV GDFRRRDIAL GGQGAPLVPA 
       160        170        180        190        200
FHHALLAHPT ERRMVLNIGG IANLSLLIPG QPVGGYDTGP GNMLMDAWIW 
       210        220        230        240        250
RQAGKPYDKD AEWARAGKVI LPLLQNMLSD PYFSQPAPKS TGREYFNYGW 
       260        270        280        290        300
LERHLRHFPG VDPRDVQATL AELTAVTISE QVLLSGGCER LMVCGGGSRN 
       310        320        330        340        350
PLLMARLAAL LPGTEVTTTD AVGISGDDME ALAFAWLAWR TLAGLPGNLP 
       360 
SVTGASQETV LGAIFPANP

Length:369

Mass (Da):39,496

Last modified:February 1, 1997 - v1

Checksum:ⁱE0B439314E60F51B

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U00096 Genomic DNA Translation: AAC74712.1 AP009048 Genomic DNA Translation: BAA15401.1
PIRⁱ	B64921
NCBI Reference Sequences More...RefSeqⁱ	NP_416157.1, NC_000913.3 WP_000835039.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaⁱ	AAC74712; AAC74712; b1640 BAA15401; BAA15401; BAA15401
GeneIDⁱ	946810
KEGGⁱ	ecj:JW1632 eco:b1640
PATRICⁱ	fig\|1411691.4.peg.620

Protein	Similar proteins		Species	Score	Length
P77570	Anhydro-N-acetylmuramic acid kinase		ECO57		369
Anhydro-N-acetylmuramic acid kinase		ECO5E		369
Anhydro-N-acetylmuramic acid kinase		ECOLX		369
Anhydro-N-acetylmuramic acid kinase		ECO5C		369
Anhydro-N-acetylmuramic acid kinase		Escherichia coli O157:H7 str. SS52		369
+591

Protein	Similar proteins		Species	Score	Length
P77570	Anhydro-N-acetylmuramic acid kinase		VIBCH		370
Anhydro-N-acetylmuramic acid kinase		VIBCL		370
Anhydro-N-acetylmuramic acid kinase		VIBCO		370
Anhydro-N-acetylmuramic acid kinase		Vibrio cholerae B33		370
Anhydro-N-acetylmuramic acid kinase		Vibrio cholerae 2740-80		370
+2138

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U00096 Genomic DNA Translation: AAC74712.1 AP009048 Genomic DNA Translation: BAA15401.1
PIRⁱ	B64921
NCBI Reference Sequences More...RefSeqⁱ	NP_416157.1, NC_000913.3 WP_000835039.1, NZ_LN832404.1

3D structure databases

ProteinModelPortalⁱ	P77570
SMRⁱ	P77570
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4263491, 14 interactors
IntActⁱ	P77570, 7 interactors
STRINGⁱ	316385.ECDH10B_1774

Proteomic databases

PaxDbⁱ	P77570
PRIDEⁱ	P77570

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC74712; AAC74712; b1640 BAA15401; BAA15401; BAA15401
GeneIDⁱ	946810
KEGGⁱ	ecj:JW1632 eco:b1640
PATRICⁱ	fig\|1411691.4.peg.620

Organism-specific databases

EchoBASEⁱ	EB3700
EcoGeneⁱ	EG13942 anmK

Phylogenomic databases

eggNOGⁱ	ENOG4105ED7 Bacteria COG2377 LUCA
HOGENOMⁱ	HOG000256309
InParanoidⁱ	P77570
KEGG Orthology (KO) More...KOⁱ	K09001
OMAⁱ	HGQTVYH
PhylomeDBⁱ	P77570

Enzyme and pathway databases

UniPathwayⁱ	UPA00343 UPA00544
BioCycⁱ	EcoCyc:G6880-MONOMER MetaCyc:G6880-MONOMER
SABIO-RKⁱ	P77570

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P77570

PROⁱ

PR:P77570

Family and domain databases

HAMAPⁱ	MF_01270 AnhMurNAc_kinase, 1 hit
InterProⁱ	View protein in InterPro IPR005338 Anhydro_N_Ac-Mur_kinase
PANTHERⁱ	PTHR30605 PTHR30605, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF03702 AnmK, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ANMK_ECOLI
Accessionⁱ	P77570Primary (citable) accession number: P77570
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
	Last sequence update:	February 1, 1997
	Last modified:	March 28, 2018
	This is version 114 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene
PATHWAY comments
Index of metabolic and biosynthesis pathways
SIMILARITY comments
Index of protein domains and families
Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P77570 (ANMK_ECOLI)

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Anhydro-N-acetylmuramic acid kinase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes an enzyme regulatory mechanism and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/enzyme_regulation' target='_top'>More...</a></p>Enzyme regulationi

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>pH dependencei

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: 1,6-anhydro-N-acetylmuramate degradation

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: peptidoglycan recycling

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

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Functionⁱ

Enzyme regulationⁱ

Kineticsⁱ

pH dependenceⁱ

Pathwayⁱ: 1,6-anhydro-N-acetylmuramate degradation

Pathwayⁱ: peptidoglycan recycling

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ