UniProtKB - P77544 (YFCF_ECOLI)
Protein
Glutathione S-transferase YfcF
Gene
yfcF
Organism
Escherichia coli (strain K12)
Status
Functioni
Exhibits glutathione (GSH) S-transferase activity toward 1-chloro-2,4-dinitrobenzene (CDNB); however this activity is as low as 1% of that of GstA. Also displays a GSH-dependent peroxidase activity toward cumene hydroperoxide. Is involved in defense against oxidative stress, probably via its peroxidase activity.1 Publication
Catalytic activityi
- EC:2.5.1.181 Publication
Activity regulationi
GSH transferase activity is inhibited by S-hexyl glutathione.1 Publication
Kineticsi
kcat is 0.11 sec(-1) for the GSH transferase reaction with CDNB as substrate.
- KM=0.24 mM for glutathione1 Publication
- KM=1.1 mM for 1-chloro-2,4-dinitrobenzene1 Publication
pH dependencei
Activity decreases as pH is lowered from neutral to acidic.1 Publication
GO - Molecular functioni
- glutathione transferase activity Source: EcoCyc
- maleylacetoacetate isomerase activity Source: GO_Central
- peroxidase activity Source: UniProtKB-KW
GO - Biological processi
- glutathione metabolic process Source: GO_Central
- L-phenylalanine catabolic process Source: GO_Central
- response to hydrogen peroxide Source: EcoCyc
Keywordsi
Molecular function | Oxidoreductase, Peroxidase, Transferase |
Enzyme and pathway databases
BioCyci | EcoCyc:G7193-MONOMER MetaCyc:G7193-MONOMER |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:yfcF Ordered Locus Names:b2301, JW2298 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Disruption phenotypei
Deletion of yfcF decreases the resistance of the bacteria to hydrogen peroxide.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 10 | S → A: Nearly no effect on GSH transferase activity. 1 Publication | 1 | |
Mutagenesisi | 16 | S → G: 2% of wild-type GSH transferase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186015 | 1 – 214 | Glutathione S-transferase YfcFAdd BLAST | 214 |
Proteomic databases
jPOSTi | P77544 |
PaxDbi | P77544 |
PRIDEi | P77544 |
Interactioni
Protein-protein interaction databases
BioGRIDi | 4260516, 12 interactors |
IntActi | P77544, 3 interactors |
STRINGi | 511145.b2301 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P77544 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P77544 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 6 – 87 | GST N-terminalAdd BLAST | 82 | |
Domaini | 95 – 214 | GST C-terminalAdd BLAST | 120 |
Sequence similaritiesi
Belongs to the GST superfamily.Curated
Phylogenomic databases
eggNOGi | COG0625, Bacteria |
HOGENOMi | CLU_011226_17_0_6 |
InParanoidi | P77544 |
PhylomeDBi | P77544 |
Family and domain databases
CDDi | cd03195, GST_C_4, 1 hit |
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR010987, Glutathione-S-Trfase_C-like IPR036282, Glutathione-S-Trfase_C_sf IPR040079, Glutathione_S-Trfase IPR004045, Glutathione_S-Trfase_N IPR034338, GST_4_C IPR036249, Thioredoxin-like_sf |
Pfami | View protein in Pfam PF14834, GST_C_4, 1 hit PF02798, GST_N, 1 hit |
SFLDi | SFLDS00019, Glutathione_Transferase_(cytos, 1 hit |
SUPFAMi | SSF47616, SSF47616, 1 hit SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS50405, GST_CTER, 1 hit PS50404, GST_NTER, 1 hit |
i Sequence
Sequence statusi: Complete.
P77544-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKPAITLWS DAHFFSPYVL SAWVALQEKG LSFHIKTIDL DSGEHLQPTW
60 70 80 90 100
QGYGQTRRVP LLQIDDFELS ESSAIAEYLE DRFAPPTWER IYPLDLENRA
110 120 130 140 150
RARQIQAWLR SDLMPIREER PTDVVFAGAK KAPLTAEGKA SAEKLFAMAE
160 170 180 190 200
HLLVLGQPNL FGEWCIADTD LALMINRLVL HGDEVPERLV DYATFQWQRA
210
SVQRFIALSA KQSG
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75361.1 AP009048 Genomic DNA Translation: BAA16138.1 |
PIRi | C65002 |
RefSeqi | NP_416804.1, NC_000913.3 WP_000042442.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC75361; AAC75361; b2301 BAA16138; BAA16138; BAA16138 |
GeneIDi | 946749 |
KEGGi | ecj:JW2298 eco:b2301 |
PATRICi | fig|1411691.4.peg.4433 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00096 Genomic DNA Translation: AAC75361.1 AP009048 Genomic DNA Translation: BAA16138.1 |
PIRi | C65002 |
RefSeqi | NP_416804.1, NC_000913.3 WP_000042442.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3BBY | X-ray | 1.85 | A | 1-214 | [»] | |
SMRi | P77544 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4260516, 12 interactors |
IntActi | P77544, 3 interactors |
STRINGi | 511145.b2301 |
Proteomic databases
jPOSTi | P77544 |
PaxDbi | P77544 |
PRIDEi | P77544 |
Genome annotation databases
EnsemblBacteriai | AAC75361; AAC75361; b2301 BAA16138; BAA16138; BAA16138 |
GeneIDi | 946749 |
KEGGi | ecj:JW2298 eco:b2301 |
PATRICi | fig|1411691.4.peg.4433 |
Organism-specific databases
EchoBASEi | EB3862 |
Phylogenomic databases
eggNOGi | COG0625, Bacteria |
HOGENOMi | CLU_011226_17_0_6 |
InParanoidi | P77544 |
PhylomeDBi | P77544 |
Enzyme and pathway databases
BioCyci | EcoCyc:G7193-MONOMER MetaCyc:G7193-MONOMER |
Miscellaneous databases
EvolutionaryTracei | P77544 |
PROi | PR:P77544 |
Family and domain databases
CDDi | cd03195, GST_C_4, 1 hit |
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR010987, Glutathione-S-Trfase_C-like IPR036282, Glutathione-S-Trfase_C_sf IPR040079, Glutathione_S-Trfase IPR004045, Glutathione_S-Trfase_N IPR034338, GST_4_C IPR036249, Thioredoxin-like_sf |
Pfami | View protein in Pfam PF14834, GST_C_4, 1 hit PF02798, GST_N, 1 hit |
SFLDi | SFLDS00019, Glutathione_Transferase_(cytos, 1 hit |
SUPFAMi | SSF47616, SSF47616, 1 hit SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS50405, GST_CTER, 1 hit PS50404, GST_NTER, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | YFCF_ECOLI | |
Accessioni | P77544Primary (citable) accession number: P77544 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | February 1, 1997 | |
Last modified: | December 2, 2020 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families