UniProtKB - P77541 (PRPB_ECOLI)
Protein
2-methylisocitrate lyase
Gene
prpB
Organism
Escherichia coli (strain K12)
Status
Functioni
Involved in the catabolism of short chain fatty acids (SCFA) via the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the thermodynamically favored C-C bond cleavage of (2R,3S)-2-methylisocitrate to yield pyruvate and succinate via an alpha-carboxy-carbanion intermediate.UniRule annotation2 Publications
Catalytic activityi
- EC:4.1.3.30UniRule annotation2 Publications
Cofactori
Mg2+UniRule annotation3 Publications
Kineticsi
Kcat is 12 sec(-1) for methylisocitrate lyase with threo-2-methylisocitrate as substrate (mixture of (2R,3S)- and (2S,3R)-2-methylisocitrate at pH 7 and 30 degrees Celsius).1 Publication
- KM=35 µM for magnesium2 Publications
- KM=19 µM for threo-2-methylisocitrate (a mixture of (2R,3S)- and (2S,3R)-2-methylisocitrate in presence of 2 mM magnesium at pH 7 and 30 degrees Celsius)2 Publications
: propanoate degradation Pathwayi
This protein is involved in the pathway propanoate degradation, which is part of Organic acid metabolism.UniRule annotation1 PublicationView all proteins of this organism that are known to be involved in the pathway propanoate degradation and in Organic acid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 85 | MagnesiumUniRule annotation2 Publications | 1 | |
Metal bindingi | 87 | MagnesiumUniRule annotation2 Publications | 1 | |
Binding sitei | 158 | SubstrateUniRule annotation1 Publication | 1 | |
Binding sitei | 188 | SubstrateUniRule annotation1 Publication | 1 | |
Binding sitei | 241 | SubstrateUniRule annotation1 Publication | 1 | |
Binding sitei | 270 | SubstrateUniRule annotation1 Publication | 1 |
GO - Molecular functioni
- magnesium ion binding Source: UniProtKB
- methylisocitrate lyase activity Source: UniProtKB
GO - Biological processi
- propionate catabolic process, 2-methylcitrate cycle Source: EcoCyc
Keywordsi
Molecular function | Lyase |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:G6196-MONOMER MetaCyc:G6196-MONOMER |
BRENDAi | 4.1.3.30, 2026 |
SABIO-RKi | P77541 |
UniPathwayi | UPA00946 |
Names & Taxonomyi
Protein namesi | Recommended name: 2-methylisocitrate lyase1 Publication (EC:4.1.3.302 Publications)Short name: 2-MIC1 Publication Short name: MICL1 Publication Alternative name(s): (2R,3S)-2-methylisocitrate lyase1 Publication |
Gene namesi | Name:prpBUniRule annotation Synonyms:yahQ Ordered Locus Names:b0331, JW0323 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 123 | C → S: Inactive. 1 Publication | 1 |
Chemistry databases
DrugBanki | DB01727, Isocitric Acid |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed2 Publications | |||
ChainiPRO_0000068815 | 2 – 296 | 2-methylisocitrate lyaseAdd BLAST | 295 |
Proteomic databases
jPOSTi | P77541 |
PaxDbi | P77541 |
PRIDEi | P77541 |
Interactioni
Subunit structurei
Homotetramer; dimer of dimers.
UniRule annotation2 PublicationsProtein-protein interaction databases
BioGRIDi | 4259808, 9 interactors |
IntActi | P77541, 12 interactors |
STRINGi | 511145.b0331 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P77541 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P77541 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 45 – 47 | Substrate bindingUniRule annotation1 Publication | 3 | |
Regioni | 123 – 124 | Substrate bindingUniRule annotation1 Publication | 2 | |
Regioni | 210 – 212 | Substrate bindingUniRule annotation1 Publication | 3 |
Sequence similaritiesi
Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family.UniRule annotationCurated
Phylogenomic databases
eggNOGi | COG2513, Bacteria |
HOGENOMi | CLU_027389_3_2_6 |
InParanoidi | P77541 |
PhylomeDBi | P77541 |
Family and domain databases
CDDi | cd00377, ICL_PEPM, 1 hit |
Gene3Di | 3.20.20.60, 1 hit |
HAMAPi | MF_01939, PrpB, 1 hit |
InterProi | View protein in InterPro IPR039556, ICL/PEPM IPR018523, Isocitrate_lyase_ph_CS IPR012695, PrpB IPR015813, Pyrv/PenolPyrv_Kinase-like_dom IPR040442, Pyrv_Kinase-like_dom_sf |
SUPFAMi | SSF51621, SSF51621, 1 hit |
TIGRFAMsi | TIGR02317, prpB, 1 hit |
PROSITEi | View protein in PROSITE PS00161, ISOCITRATE_LYASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P77541-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSLHSPGKAF RAALTKENPL QIVGTINANH ALLAQRAGYQ AIYLSGGGVA
60 70 80 90 100
AGSLGLPDLG ISTLDDVLTD IRRITDVCSL PLLVDADIGF GSSAFNVART
110 120 130 140 150
VKSMIKAGAA GLHIEDQVGA KRCGHRPNKA IVSKEEMVDR IRAAVDAKTD
160 170 180 190 200
PDFVIMARTD ALAVEGLDAA IERAQAYVEA GAEMLFPEAI TELAMYRQFA
210 220 230 240 250
DAVQVPILAN ITEFGATPLF TTDELRSAHV AMALYPLSAF RAMNRAAEHV
260 270 280 290
YNVLRQEGTQ KSVIDTMQTR NELYESINYY QYEEKLDNLF ARSQVK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U73857 Genomic DNA Translation: AAB18055.1 U00096 Genomic DNA Translation: AAC73434.1 AP009048 Genomic DNA Translation: BAE76114.1 |
PIRi | C64760 |
RefSeqi | NP_414865.1, NC_000913.3 WP_000052206.1, NZ_LN832404.1 |
Genome annotation databases
EnsemblBacteriai | AAC73434; AAC73434; b0331 BAE76114; BAE76114; BAE76114 |
GeneIDi | 944990 |
KEGGi | ecj:JW0323 eco:b0331 |
PATRICi | fig|1411691.4.peg.1945 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U73857 Genomic DNA Translation: AAB18055.1 U00096 Genomic DNA Translation: AAC73434.1 AP009048 Genomic DNA Translation: BAE76114.1 |
PIRi | C64760 |
RefSeqi | NP_414865.1, NC_000913.3 WP_000052206.1, NZ_LN832404.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1MUM | X-ray | 1.90 | A/B | 2-296 | [»] | |
1MZX | model | - | A | 3-290 | [»] | |
1OQF | X-ray | 1.93 | A/B | 2-296 | [»] | |
1XG3 | X-ray | 1.90 | A/B/C/D | 2-296 | [»] | |
1XG4 | X-ray | 1.60 | A/B/C/D | 2-296 | [»] | |
SMRi | P77541 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4259808, 9 interactors |
IntActi | P77541, 12 interactors |
STRINGi | 511145.b0331 |
Chemistry databases
DrugBanki | DB01727, Isocitric Acid |
Proteomic databases
jPOSTi | P77541 |
PaxDbi | P77541 |
PRIDEi | P77541 |
Genome annotation databases
EnsemblBacteriai | AAC73434; AAC73434; b0331 BAE76114; BAE76114; BAE76114 |
GeneIDi | 944990 |
KEGGi | ecj:JW0323 eco:b0331 |
PATRICi | fig|1411691.4.peg.1945 |
Organism-specific databases
EchoBASEi | EB3370 |
Phylogenomic databases
eggNOGi | COG2513, Bacteria |
HOGENOMi | CLU_027389_3_2_6 |
InParanoidi | P77541 |
PhylomeDBi | P77541 |
Enzyme and pathway databases
UniPathwayi | UPA00946 |
BioCyci | EcoCyc:G6196-MONOMER MetaCyc:G6196-MONOMER |
BRENDAi | 4.1.3.30, 2026 |
SABIO-RKi | P77541 |
Miscellaneous databases
EvolutionaryTracei | P77541 |
PROi | PR:P77541 |
Family and domain databases
CDDi | cd00377, ICL_PEPM, 1 hit |
Gene3Di | 3.20.20.60, 1 hit |
HAMAPi | MF_01939, PrpB, 1 hit |
InterProi | View protein in InterPro IPR039556, ICL/PEPM IPR018523, Isocitrate_lyase_ph_CS IPR012695, PrpB IPR015813, Pyrv/PenolPyrv_Kinase-like_dom IPR040442, Pyrv_Kinase-like_dom_sf |
SUPFAMi | SSF51621, SSF51621, 1 hit |
TIGRFAMsi | TIGR02317, prpB, 1 hit |
PROSITEi | View protein in PROSITE PS00161, ISOCITRATE_LYASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PRPB_ECOLI | |
Accessioni | P77541Primary (citable) accession number: P77541 Secondary accession number(s): Q2MC92 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 1, 1997 |
Last sequence update: | January 23, 2007 | |
Last modified: | April 7, 2021 | |
This is version 154 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families