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Entry version 150 (02 Jun 2021)
Sequence version 1 (01 Feb 1997)
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Protein

L-cystine transporter TcyP

Gene

tcyP

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Involved in L-cystine import (PubMed:25139244, PubMed:25837721, PubMed:26350134, PubMed:27481704).

Low affinity L-cystine transporter that is mainly involved in L-cystine uptake from outside as a nutrient (PubMed:25837721).

Is the primary transporter at high concentrations of cystine (PubMed:26350134).

Probably functions as a proton symporter (Probable). Can also transport D-cystine, cysteine and homocystine as a methionine precursor (PubMed:25139244, PubMed:26350134).

Mediates accumulation of the toxic compounds L-selenaproline (SCA) and L-selenocystine (SeCys) (PubMed:25139244).

Can also transport S-sulfocysteine and is required for growth on S-sulfocysteine as a sulfur source (PubMed:27481704).

Plays the principal role in the 3-mercaptopyruvate sulfurtransferase (3MST)-mediated generation of endogenous hydrogen sulfide (H2S) (PubMed:28533366).

1 Publication5 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Inhibited by L-cystine and L-cysteine, and to a lesser extent by D-cystine.1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=1.1 µM for cystine1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processAmino-acid transport, Symport, Transport

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
EcoCyc:G6934-MONOMER
MetaCyc:G6934-MONOMER

Protein family/group databases

Transport Classification Database

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TCDBi
2.A.23.1.8, the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
L-cystine transporter TcyPCurated
Alternative name(s):
S-sulfocysteine transporter1 Publication
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:tcyP1 Publication
Synonyms:ydjN
Ordered Locus Names:b1729, JW1718
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/subcellular_location_section">'Subcellular location'</a> section describes the extent of a membrane-spanning region of the protein. It denotes the presence of both alpha-helical transmembrane regions and the membrane spanning regions of beta-barrel transmembrane proteins.<p><a href='/help/transmem' target='_top'>More...</a></p>Transmembranei1 – 21HelicalSequence analysisAdd BLAST21
Transmembranei33 – 53HelicalSequence analysisAdd BLAST21
Transmembranei73 – 93HelicalSequence analysisAdd BLAST21
Transmembranei106 – 126HelicalSequence analysisAdd BLAST21
Transmembranei185 – 205HelicalSequence analysisAdd BLAST21
Transmembranei264 – 284HelicalSequence analysisAdd BLAST21
Transmembranei347 – 367HelicalSequence analysisAdd BLAST21
Transmembranei370 – 390HelicalSequence analysisAdd BLAST21
Transmembranei395 – 415HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Disruption of the gene decreases uptake of cystine (PubMed:25837721). The mutant cannot grow on S-sulfocysteine (PubMed:27481704). The tcyP-tcyJ double mutant is unable to import cystine (PubMed:26350134, PubMed:27481704). The tcyP-tcyL double mutant cannot grow in the minimal medium containing L-cystine as a sole sulfur source (PubMed:25837721). The tcyP-tcyJ and tcyP-tcyL double mutants are completely resistant to both L-selenaproline and L-selenocystine (PubMed:25139244).4 Publications

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002021211 – 463L-cystine transporter TcyPAdd BLAST463

Proteomic databases

jPOST - Japan Proteome Standard Repository/Database

More...
jPOSTi
P77529

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P77529

PRoteomics IDEntifications database

More...
PRIDEi
P77529

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section reports the experimentally proven effects of inducers and repressors (usually chemical compounds or environmental factors) on the level of protein (or mRNA) expression (up-regulation, down-regulation, constitutive expression).<p><a href='/help/induction' target='_top'>More...</a></p>Inductioni

Transcription is controlled by CysB (PubMed:26350134, PubMed:27481704, PubMed:28533366). Highly expressed in sulfate medium and repressed in cystine medium (PubMed:26350134).3 Publications

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

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BioGRIDi
4259522, 189 interactors

Database of interacting proteins

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DIPi
DIP-11776N

STRING: functional protein association networks

More...
STRINGi
511145.b1729

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
COG1823, Bacteria

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_019375_0_1_6

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
P77529

Database for complete collections of gene phylogenies

More...
PhylomeDBi
P77529

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.10.3860.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR001991, Na-dicarboxylate_symporter
IPR036458, Na:dicarbo_symporter_sf

The PANTHER Classification System

More...
PANTHERi
PTHR42865, PTHR42865, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00375, SDF, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF118215, SSF118215, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P77529-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNFPLIANIV VFVVLLFALA QTRHKQWSLA KKVLVGLVMG VVFGLALHTI
60 70 80 90 100
YGSDSQVLKD SVQWFNIVGN GYVQLLQMIV MPLVFASILS AVARLHNASQ
110 120 130 140 150
LGKISFLTIG TLLFTTLIAA LVGVLVTNLF GLTAEGLVQG GAETARLNAI
160 170 180 190 200
ESNYVGKVSD LSVPQLVLSF IPKNPFADLT GANPTSIISV VIFAAFLGVA
210 220 230 240 250
ALKLLKDDAP KGERVLAAID TLQSWVMKLV RLVMQLTPYG VLALMTKVVA
260 270 280 290 300
GSNLQDIIKL GSFVVASYLG LLIMFAVHGI LLGINGVSPL KYFRKVWPVL
310 320 330 340 350
TFAFTSRSSA ASIPLNVEAQ TRRLGVPESI ASFAASFGAT IGQNGCAGLY
360 370 380 390 400
PAMLAVMVAP TVGINPLDPM WIATLVGIVT VSSAGVAGVG GGATFAALIV
410 420 430 440 450
LPAMGLPVTL VALLISVEPL IDMGRTALNV SGSMTAGTLT SQWLKQTDKA
460
ILDSEDDAEL AHH
Length:463
Mass (Da):48,662
Last modified:February 1, 1997 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i7D45D8A354D2885C
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

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EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

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DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74799.1
AP009048 Genomic DNA Translation: BAA15509.1

Protein sequence database of the Protein Information Resource

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PIRi
A64932

NCBI Reference Sequences

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RefSeqi
NP_416243.1, NC_000913.3
WP_001010696.1, NZ_SSZK01000001.1

Genome annotation databases

Ensembl bacterial and archaeal genome annotation project

More...
EnsemblBacteriai
AAC74799; AAC74799; b1729
BAA15509; BAA15509; BAA15509

Database of genes from NCBI RefSeq genomes

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GeneIDi
946238

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
ecj:JW1718
eco:b1729

Pathosystems Resource Integration Center (PATRIC)

More...
PATRICi
fig|1411691.4.peg.527

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74799.1
AP009048 Genomic DNA Translation: BAA15509.1
PIRiA64932
RefSeqiNP_416243.1, NC_000913.3
WP_001010696.1, NZ_SSZK01000001.1

3D structure databases

Database of comparative protein structure models

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ModBasei
Search...

SWISS-MODEL Interactive Workspace

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SWISS-MODEL-Workspacei
Submit a new modelling project...

Protein-protein interaction databases

BioGRIDi4259522, 189 interactors
DIPiDIP-11776N
STRINGi511145.b1729

Protein family/group databases

TCDBi2.A.23.1.8, the dicarboxylate/amino acid:cation (na(+) or h(+)) symporter (daacs) family

Proteomic databases

jPOSTiP77529
PaxDbiP77529
PRIDEiP77529

Genome annotation databases

EnsemblBacteriaiAAC74799; AAC74799; b1729
BAA15509; BAA15509; BAA15509
GeneIDi946238
KEGGiecj:JW1718
eco:b1729
PATRICifig|1411691.4.peg.527

Organism-specific databases

EchoBASE - an integrated post-genomic database for E. coli

More...
EchoBASEi
EB3746

Phylogenomic databases

eggNOGiCOG1823, Bacteria
HOGENOMiCLU_019375_0_1_6
InParanoidiP77529
PhylomeDBiP77529

Enzyme and pathway databases

BioCyciEcoCyc:G6934-MONOMER
MetaCyc:G6934-MONOMER

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P77529

Family and domain databases

Gene3Di1.10.3860.10, 1 hit
InterProiView protein in InterPro
IPR001991, Na-dicarboxylate_symporter
IPR036458, Na:dicarbo_symporter_sf
PANTHERiPTHR42865, PTHR42865, 1 hit
PfamiView protein in Pfam
PF00375, SDF, 1 hit
SUPFAMiSSF118215, SSF118215, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiTCYP_ECOLI
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P77529
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: February 1, 1997
Last modified: June 2, 2021
This is version 150 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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